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International Journal of Applied Biology
Published by Universitas Hasanuddin
ISSN : 25802410     EISSN : 25802119     DOI : -
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
Articles 33 Documents
Biofiltration Efficiency of Algae Spirogyra hyalina to Reduce Salinity of Sea Water Putra, Ayub Wirabuana
International Journal of Applied Biology Vol 1, No 1 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (180.395 KB)

Abstract

Algae Spyrogyra hyalina is one species of fresh water algae that is very easy to find in Indonesia. This species of algae known to have a lot of potential, which until now unknown. One potential that tried to explore in this research is the ability of these algae for decreasing the level of salt water salinity into fresh water. This research is an experiment with a completely randomized design (CRD), three treatments and three replications. Testing is done by performing algae arbsorbtion using three forms (powder, granule, and paper) with different biomass (1 g, 2 g, and 4gr). Preparations then coupled into a transparent pipe. The results showed the real effect of the decreased levels of salinity brine through the circuit. Filter with the powder forms with 4gr biomass has the highest salinity level reduction of 15%o,  then the dosage form of granules with biomass 4gr with decreased 5.23%o and the third dosage form of paper with 1 gram of biomass with a decrease of 4.23%o.Forms with other biomass have a decrease in the salinity level of not more than 4%o. Preparations with powder form and 4gr biomass can be used to reduce the salinity level in the salt water.
Diversity of Invertebrate Epifaunas Associating with Mangrove In Balangdatu Village Tanakeke Islands of South Sulawesi Crisnawati, Crisnawati; Litaay, Magdalena; Prisambodo, Dody; Santoso, Slamet
International Journal of Applied Biology Vol 1, No 1 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Research on the diversity of invertebrate epifaunas associating with mangroves in the Balangdatu village of Tanakeke regency, Takalar district, South Sulawesi, has been conducted from September to December 2016. The objective of this research was to know the diversity of invertebrate epifaunas associating with mangrove in Balangdatu Village of Tanakeke Islands. Sample collection was done by using explorative quantitatively plot methods with size 2 x 2 m, applied at three different stations. Data analysis includes calculation of Absolute density, Uniformity Index (Shannon-wiener), Dominance Index, and Distribution Index. The results showed that there were 13 species of epi fauna of 9 tribes, namely Littoraria sp 1, Cerithidea cingulata, Littoraria sp 2, Littoraria pallescens, Episesarma sp, Saccostrea cucullata, Pagurus sp, Terebralia sulcata, Cerithidea sp, Nerita planospira, Nisto histrio, Littoraria scabra, and Cassidula vespertilionis. The highest density was found in Littoraria scabra with a density of 0.72 ind/m2. Diversity indices are low which indicate depressed environmental conditions. Distribution Index values across stations <1 indicating that the pattern of individual dispersion tends to be uniform.
The Production of Chitosan from Shrimp Shell Waste and Its Formulation in Patch DosageForm Combined with Aloe vera Extract as Antiinfection Agent Haeriah, Haeriah; Rahmatullah, Muhammad; Indardaya, Andi; Utomo, Emilia; Novianti, Novianti; Sartini, Sartini
International Journal of Applied Biology Vol 1, No 1 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Chitosan can be obtained from chitin isolated from shrimp waste through the process of deacetylation of chitin. Chitosan can be used as a patch base material because of its biocompatibility and biodegradability, and has antibacterial activity. The combination of Chitosan and Aloe vera extracts may be useful in patch dosage forms as wound dressings that have antiinfective activity. The research methods was laboratory experimental which consists of : chitosan was obtained from shell waste sequentially by deproteinisation, demineralisation, and deacetylation processes, and analyzed its characteristic, respectivel; Aloe vera gel was extraction by maceration methods using ethanol as solvent; patches were formulated using 2 % chitosan in 1.5 % glacial acetic acid,  1.6 %  Aloe vera extracts and 10 % glycerin and evaluated its physical propoperties,  skin iritation test, and antibacterial test against S.aureus. The results showed that percentage yield of chitosan was 32.67%, with characteristics; Its viscosity 93.33 cps, pH 4, 30 % deacetylation degree.  Percentage yield of Aloe vera  extract was 22.73%, containing acemannan, and had antibacterial activity against S.aureus.  The result of patch evaluation showed that the thickness of patch was 0.45 mm, 114 times folding endurance, moisture uptake of 45.03%, moisture content of 23.64%, safe to use, and  diameter of inhibition zone was 6.39 mm against S.aureus. So, combination of Aloe vera and Chitosan from shrimp shell as patch may use as antiinfection wound healing.  Keywords : Aloe vera L.; chitosan; wound dressing; patch; shrimp shell waste
A Study on Entrapment Efficiency of Earthworms (Lumbricus rubellus) Extract in the Ethosomal Drug Delivery System Pratiwi, Sabrina Resky; Ainiah, Nur; Hardyanti, Hardyanti; Putri, Dini Rusdayanti; Utomo, Emilia
International Journal of Applied Biology Vol 1, No 1 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

IntroductionEarthworms (Lumbricus rubellus) extract is known to contain  bioactive protein as antibacterial compounds. One of the disadvantages of polar compounds is slow penetration into the skin layers which can be solved by formulating it in the form of ethosomal drug delivery system. The aims of this research was to get information about ethanol concentration that can give the highest entrapment efficiency of the ethosome. MethodsEarthworms powder was macerated using 50% ethanol for 3 days. The extract was  formulated into ethosome with variation of ethanol concentration that are 20%, 30%, 40%, and 50%, respectively. The measurement of entrapment efficiency was conducted by measuring the amount of active protein of earthworms extract that was entrapped in ethosome vesicles using spectrophotometer UV-Visible.ResultsThe results showed that the highest entrapment efficiency was 72.58 % performed by formula which was made with 0.5 % extract, 2 % soy lecithin and 50 % ethanol.ConclusionsThe highest percentage of entrapment efficiency of earthworms extract ethosome was 72.58%.Keywords : ethosome, ethanol concentration , entrapment efficiency, Lumbricus rubellus.  
Application of Cigeratte Filter from Algae Spyrogira peipingensis to Reduce Nicotine, Tar, and Carbon Monoxide Bahri, Syamsul
International Journal of Applied Biology Vol 1, No 1 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Algae Spirogyra peipingensis is a group of green algae from the order Zygnematales. Spirogyra algae serves as a bioremediation agent. In addition Spirogyra algae has great potential in reducing toxin levels in cigarette smoke. Because the biomass of alga Spirogyra has an irregular shaped surface texture and consists of amino acid, amide, carboxylic, hydroxyl, and carbonyl group that can bind various heavy metals and other harmful compounds. Alga Spirogyra peipingensis samples used are algae from Bone, South Sulawesi. Prior to the trial, the first pretreatment and processed to become powder. Algae powder was then applied to cigarette filters and tested on several respondents. Results showed that there was a significant decrease  tar, nicotine and carbon monoxide levels in cigarette smoke by using Spirogyra peipingensis algae but the most significant decrease of carbon monoxide, while tar and nicotine decreased even though not as high as carbon monoxide. 
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Sumiasih, Inanpi Hidayati; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
Effect of Yeast Based Biofertilizer combined with bacteria on Mustard Plant Growth Alami, Nur Hidayatul
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Biofertilizers are commonly defined as microbial inoculants which are capable of mobilizing important nutrient in the soil from non-available to available form for the plants through their biological processes. Hence to increase the productivity of the soil, the use of biofertilizer is necessary. Candida yeast as a phosphate solubilizer and decomposer. Azotobacter bacteria as a nitrogen-fixing bacteria and Bacillus as a phosphate solubilizer. The combination of these microorganisms as biofertilizer needs to be tested its effect against mustard (Brassica juncea L.) plant growth.The results showed that the combination of Bacillus Candida obtained the highest yield among other biofertilizer treatments with a value of 13.6 cm at plant height, 4.96 grams in wet weight and 2.29 grams in dry weight. The combination of Azotobacter Candida showed the highest result on the parameter of width and number of leaves with the values of 20.3 cm and 5.3. Combination of Azotobacter Bacillus Candida showed the highest yield on the root length parameter with a value of 5.0 cm. The addition of biofertilizer is expected to reduce pollution on the soil due to residues produced by chemical fertilizers.Keyword : Azotobacter, Bacillus, Biofertilizer, Candida, Mustard Plant
Study of Lacid Acid Bacteria Activities from Human Oral Mucosa for Candida albicans Inhibition Lauw, Evi
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Lactic acid bacteria are known to have potential in producing antimicrobial compounds, including antifungal agents. This study aims to determine whether the lactic acid bacteria from human oral mucosa have the potential to produce the secondary metabolite to inhibit the candidiasis, which caused by pathogen fungi, Candida albicans. Lactic acid bacteria were isolated from three healthy respondents based on age difference that is an infant, child, and adult. Lactic acid bacteria isolation using the deMann Rogosa Sharpe Broth (MRSB)-liquid medium, followed by purification stage with the quadrant scratch method using the deMann Rogosa Sharpe Agar (MRSA)-solid medium. The purified isolates obtained were characterized and identified by Gram staining, biochemical tests, and inhibition zone diameter measurement toward pathogenic fungi Candida albicans. The results were obtained from each of the two lactic acid bacteria isolates from each respondent with characteristics including gram-positive bacteria with short stem cell shape, possibly as Lactobacillus sp. It is conclude that the lactic acid bacteria of oral mucosa from adult had greater anticandidal activity than infant and child. Keywords: lactic acid bacteria, human oral mucosa, antifungal activity, Candida albicans
Macrozoobenthos Community Structure in the Coastal Waters of Marsegu Island, Maluku, Indonesia Yunita, Fitry; Leiwakabessy, Fredy; Rumahlatu, Dominggus
International Journal of Applied Biology Vol 2, No 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (667.136 KB) | DOI: 10.20956/ijab.v2i1.3224

Abstract

The existence of macrozoobenthos in an ecosystem is closely related to the conditions of the surrounding environment. This research aimed to examine the physical chemical condition of aquatic environment and macrozoobenthos community structure in the coastal waters of Marsergu Island. This research was conducted in July and December 2016 in 3 stations. The data were collected by using sampling technique to measure the environmental factors (temperature, salinity, pH, and dissolved oxygen of seawater) and enumeration of gastropod species with in-situ. The results of this research showed that macrozoobenthos in the coastal waters of Marsegu Island consisted of 2 Phylum, namely Molusca and Echinoderms, that is, gastropod with 18 species, bivalvia with 5 species, and echinoderms with 17 species. Echinoderms was the dominant macrozoobentos with a percentage of 45%, followed by gastropods 42%, and bivalvia 13%. This suggests that echinoderms and gastropods are the main components that make up the macrozoobenthos community structure in the coastal waters of Marsegu Island. The results of the calculation of the diversity index of macrozoobenthos in the three research stations showed the highest score was station 1 as much as 3.56, station 2 as much as 3.52 and the lowest was station 3 as much as 2.07.
Biofiltration Efficiency of Algae Spirogyra hyalina to Reduce Salinity of Sea Water Putra, Ayub Wirabuana
International Journal of Applied Biology Vol 1, No 1 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Original Source | Check in Google Scholar | Full PDF (180.395 KB) | DOI: 10.30597/ijab.v1i1.2003

Abstract

Algae Spyrogyra hyalina is one species of fresh water algae that is very easy to find in Indonesia. This species of algae known to have a lot of potential, which until now unknown. One potential that tried to explore in this research is the ability of these algae for decreasing the level of salt water salinity into fresh water. This research is an experiment with a completely randomized design (CRD), three treatments and three replications. Testing is done by performing algae arbsorbtion using three forms (powder, granule, and paper) with different biomass (1 g, 2 g, and 4gr). Preparations then coupled into a transparent pipe. The results showed the real effect of the decreased levels of salinity brine through the circuit. Filter with the powder forms with 4gr biomass has the highest salinity level reduction of 15%o,  then the dosage form of granules with biomass 4gr with decreased 5.23%o and the third dosage form of paper with 1 gram of biomass with a decrease of 4.23%o.Forms with other biomass have a decrease in the salinity level of not more than 4%o. Preparations with powder form and 4gr biomass can be used to reduce the salinity level in the salt water.

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