International Journal of Applied Biology
Published by Universitas Hasanuddin
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal’s Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
Articles
33
Articles
Diversity of Ordo Lepidoptera In Mangkok Resort, Sebangau National Park, Central Kalimantan

Faqih, Achmad

International Journal of Applied Biology Vol 2, No 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

The diversity of the butterfly in Sebangau National Park, particularly in the Mangkok Resort area has different morphological features in each species. The differences distinguish each species between families. Steps to know the diversity species or populations can be measured and identified based on similarities or differences in Shannon-Wieners diversity analysis. This research was conducted by random purposive sampling using insect net and food trap. Observation and sampling were conducted in the area of natural forest and forest restoration during the dry and rainy seasons. The results showed, the species obtained and identified as many as 25 species from 4 families namely Papilionidae, Nymphalidae, Pieridae and Lycaenidae. Butterflies obtained from food trap are 24 individuals from 5 species. The level of diversity, evenness and richness of the species of butterflies in each region during the rainy and dry seasons which are the location of the study are included in the "medium" category with the average value of the diversity index, the evenness and the richness of the butterfly species of 2.502. The relative dominance and relative abundance of the butterfly species in the Mangkok resort shows no species communities that are extreme dominant from species other.

Ligation, Transformation and Characterization of Rv 1926c Mycobacterium tuberculosis to Escherichia coli JM 109 For Latent Tuberculosis Immunodiagnostic

Agus, Rosana

International Journal of Applied Biology Vol 2, No 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Tuberculosis caused by Mycobacterium tuberculosis is the biggest infectious disease causing human death in the world. The main challenge in controlling tuberculosis is to quickly and accurately diagnose tuberculosis infection. Several kits have been produced to diagnose tuberculosis, but have different sensitivity and specificity. This shows that the kit is not yet ideal for diagnosing tuberculosis, so the search for candidates for specific antigens still needs to be done. One potential antigen is the Rv 1926c encoding MPT 63 protein. This protein is known to induce Th1 cells and produce IFN λ from PBMC cells of patients infected with tuberculosis. The purpose of this study was to clone the Rv 1926c from Mycobacterium tuberculosis as a tuberculosis immunodiagnostic kit. The method used is isolating Rv 1926c with PCR, ligation to pGEM-T vector and transformation to E.coli host cell JM 109. Clone characterization was carried out by PCR and migration analysis. The results obtained are the recombinant clones obtained have successfully inserted with the Rv 1926 c

Agronomic performance and genotypic diversity for morphological traits among early maize genotypes

Adhikari, Bhim Nath, Shrestha, Jiban, Dhakal, Bishal, Joshi, Bishnu Prasad, Bhatta, Naba Raj

International Journal of Applied Biology Vol 2, No 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Detailed information on the genetic diversity between maize germplasm (Zea mays L.) is useful for their systematic and efficient use in breeding programs. Fourteen early maize genotypes were studied to assess their performance and genotypic diversity at Doti, Nepal in 2015. Days to tasseling, days to silking, plant height, ear height, ear length, ear diameter and grain yield were significant among genotypes. Genotype SO3TEY-PO-BM, COMPOL-NIOBP and ACROSS-99402 were found higher yielder with earlier maturity. Days to tasseling (0.85), days to silking (0.82), plant height (0.79), ear length (0.71) and ear diameter (0.66) were found highly heritable traits. Grain yield (0.39) and ear height (0.47) medium and remaining traits showed low heritability. High PCV was observed for grain yield (35.10%), number of plants/plot (34.46%), tesseling silking interval (26.85%), harvested ears/plot (24.45%) and husk cover rating (22.85%) where other traits showed medium to low PCV. Grain yield showed high GCV (21.96%), ear height and husk cover had medium and remaining traits showed low GCV (<10%). Plant height (r₌0.498), harvested plants/plot (r₌0.412), harvested ear/plot (r₌0.762), ear length (r₌0.472) and ear diameter (r₌0.470) showed significant positive correlation with grain yield. The yield can be improved if selection applied in favor of those yield components.

Vegetation structure, species diversity, and mangrove zonation patterns in the Tanjung Panjang Nature Reserve Area, Gorontalo, Indonesia

Baderan, Dewi Wahyuni, Utina, Ramli, Lapolo, Nuraini

International Journal of Applied Biology Vol 2, No 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

This study aimed to analyze the structure of vegetation, diversity and patterns of mangrove zonation in the Tanjung Panjang Nature Reserve area. Data collection of mangrove vegetation structure was carried out by using the line transect method (plot measuring 20 m x 20 m), measurement of environmental parameters that supported mangrove life, and 12 soil samples carried out by laboratory tests. The sample distribution was 18 research sample points. The results showed that mangrove vegetation density both at the level of trees, saplings and seedlings was in the category of total damage with density values at tree level 0.04-0.22 ind/ha), sapling 0- 0.07ind/ha), and seedlings 0.02-0.08 ind/Ha). The percentage of closure types ranged from 0% - 38%, meaning that the criteria of damage was rare, which was <50%, and some were included in the criteria of total damage, which was 0%. The index of mangrove species diversity was sequential starting from the level of trees, saplings and seedlings, which were 0.37-1.53, 0.00-0.31, and 0.00, where the tree level diversity index was in the medium category, and the sapling and seedling levels were in the low category.

Productivity of maize (Zea mays L.) as affected by varieties and sowing dates

Gurung, Dil Bahadur, Bhandari, Balram, Shrestha, Jiban, Tripathi, Mahendra Prasad

International Journal of Applied Biology Vol 2, No 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Genotypic yield potential of maize varieties is greatly affected by sowing dates. In order to investigate the effects of sowing dates and varieties on the grain yield of maize, the field experiment was carried out at research field of National Maize Research Program (NMRP), Rampur, Chitwan, Nepal from April 2009 to March 2010.  Three varieties namely Rampur Composite, Arun-2 and Gaurav were sown at every week. The results of experiment showed that interaction effect of variety and sowing date on grain yield of maize was significant. Rampur Composite produced highest grain yield (6.1 t/ha) in August and lowest yield (2.6 t/ha) in May. Similarly Arun-2 produced highest yield (4.6 t/ha) in August and lowest yield (2.1 t/ha) in May. Gaurav produced highest grain yield (5.1 t/ha) in September followed by 4.9, 4.8 and 4.6 t/ha in February, July and August respectively and lowest yield (1.5 t/ha) in November. The sowing date was highly significant on grain production. The highest grain production was 5.1 t/ha in August followed by in February (4.9 t/ha), September (4.6 t/ha) and March (4.4 t/ha) respectively. The lowest grain yield was produced in May (2.4 t/ha). Therefore it was concluded that August planting was best for higher grain production of maize varieties (Rampur Composite, Arun-2 and Gaurav) in terai region of Nepal.

Bioaccumulation Monitoring of Heavy Metal Chromium (Cr) in Fish Oreochromis mossambicus in The Floating Net Cultivation in Sidoarjo Krembangan River

kristianto, sonny

International Journal of Applied Biology Vol 2, No 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

ABSTRACTFloating Net is a simple alternative cultivation methods. The river there is a garden in the Krembangan District Sidarjoi which is used as a place of cultivation of  Floating net fish oreochromis mossambicus (Oreochromis mossambicus), on the other hand is where waste disposal by the local community and some industries . This research aims to know the accumulation of heavy metals chromium (Cr) in the flesh of fish oreochromis mossambicus (Oreochromis mossambicus) and find out the feasibility of fish consumption oreochromis mossambicus at floating net on the river Krembangan Sidoaarjo. This research are observatif by using Purposive Random Sampling techniques. test the content of heavy metal chromium (Cr) in the flesh of fish oreochromis mossambicus (Oreochromis mossambicus) method using AAS (Atomic Absorbtion Spectrofotometry). The data generated in the analysis in quantitative descriptive.  In this research show that the heavy metal content of chromium (Cr) in the flesh of fish oreochromis mossambicus highs station III of 0.52 mg/Kg; I station of 0.51 mg/Kg II station of 0.48 mg/Kg on a sampling sedangkang the morning, at night the highest value on the station III of 0.50 mg/Kg; I station of 0.46 mg/Kg and the II of 0.39 mg/Kg. Average deposits of heavy metal Cr on fish oreochromis mossambicus (o. mossambicus) is still under the raw quality of the already established by the Director-General of POM 1989 i.e. amounted to 2.5 mg/kg but fall into the category bioakumulasi medium, so that fish oreochromis mossambicus is still worthy of consumption, but need to watch out for, considering the Cr are toxic, carcinogenic, bioakumulatif and biomagnifikasi would potentially interfere with health.  

Identification of Pseudomonas fluorescens using different biochemical tests

Nepali, Bikram, Bhattarai, Sabin, Shrestha, Jiban

International Journal of Applied Biology Vol 2, No 2 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P.  fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens  per seed which is necessary for better seedling growth and effective biological control of pathogens.

Macrozoobenthos Community Structure in the Coastal Waters of Marsegu Island, Maluku, Indonesia

Yunita, Fitry, Leiwakabessy, Fredy, Rumahlatu, Dominggus

International Journal of Applied Biology Vol 2, No 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

The existence of macrozoobenthos in an ecosystem is closely related to the conditions of the surrounding environment. This research aimed to examine the physical chemical condition of aquatic environment and macrozoobenthos community structure in the coastal waters of Marsergu Island. This research was conducted in July and December 2016 in 3 stations. The data were collected by using sampling technique to measure the environmental factors (temperature, salinity, pH, and dissolved oxygen of seawater) and enumeration of gastropod species with in-situ. The results of this research showed that macrozoobenthos in the coastal waters of Marsegu Island consisted of 2 Phylum, namely Molusca and Echinoderms, that is, gastropod with 18 species, bivalvia with 5 species, and echinoderms with 17 species. Echinoderms was the dominant macrozoobentos with a percentage of 45%, followed by gastropods 42%, and bivalvia 13%. This suggests that echinoderms and gastropods are the main components that make up the macrozoobenthos community structure in the coastal waters of Marsegu Island. The results of the calculation of the diversity index of macrozoobenthos in the three research stations showed the highest score was station 1 as much as 3.56, station 2 as much as 3.52 and the lowest was station 3 as much as 2.07.

Production of Bioethanol From Kappaphycus alvarezii Algae by Using Pichia kudriavzevii Yeast

Fadillah, Nur, Taufan, WInda Lestari, Aska, Muh Syahdan

International Journal of Applied Biology Vol 2, No 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

Kappaphycus alvarezii is a red algae that can be used as alternative raw material for bioethanol production. This is because K. alvarezii contains a high carbohydrate that reaches 60%. This study aims to determine the effect of fermentation nutrition and fermentation duration of hydrolysis results of K. alvarezii algae on pH changes, sugar levels, cell biomass and ethanol content. There are two methods was used in this research, namely hydrolysis method and fermentation method. The hydrolysis method was used α-amylases enzyme which is 150 KNU/L with 0.5%, 1.0%, 1.5% and 2.0% concentrations. The next method, K. alvarezii algae was fermented by Pichia kudriavzevii and used Gandasil-D® as an anorganic suplementation and yeast extract which is 0 g/L, 1 g/L, 2 g/L and 3 g/L. The results showed that         K. alvarezii can be fermented into bioethanol after enzymatic hydrolysis process. The optimal ethanol content was produced at 48 hours of incubation duration.

Water Feasibility Study of Bengawan Solo River for Irrigation: The Need for Technology to Solve Rice Field Pollution in Sragen, Indonesia

Prajoko, Setiyo

International Journal of Applied Biology Vol 2, No 1 (2018): International Journal of Applied Biology
Publisher : Hasanuddin University

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Abstract

The aims of this study are to determine the feasibility of Bengawan Solo River water for irrigation of rice field in Sragen, Central Java and to find solution to the problem. The data used in this study were obtained from various literatures related to the issues discussed. The collected data were then selected and analyzed with descriptive argumentative. The result indicates that the water quality of Bengawan Solo River has dropped below the water quality standard grade IV according to Government Regulation Number 82 Year 2001. The use of water from this river has caused the quality of rice to decline below the rice quality standard according to Indonesian National Standard (SNI) 01.6128-2008 due to the bioaccumulation of heavy metals on rice crops, i.e. copper (Cu), mercury (Hg), cadmium (Cd), and Chromium (Cr) exceeded the threshold. Solution proposed to overcome this problem is with counseling programs to farmers conducted by relevant agencies on the use of rice field pollution control technology by physics, chemistry, and biology.