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Dental Journal (Majalah Kedokteran Gigi)
Published by Universitas Airlangga
ISSN : 19783728     EISSN : 24429740     DOI : -
Core Subject : Health,
The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.
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Articles 11 Documents
Search results for , issue " Vol 48, No 1 (2015): (March 2015)" : 11 Documents clear
The effect of 1,25-dihydroxyvitamin D3 on MSX2 gene expression during tooth and alveolar bone development Ruspita, Intan
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p43-47

Abstract

Background: 1,25-dihydroxyvitamin D3 has been proven to be able to control the formation and biomineralization of tissue through a regulatory gene. A previous research even showed that a cell responsible for the formation of the enamel (ameloblasts), dentin (odontoblasts) and bone (osteoblasts, osteoclasts) was the target of  1,25-dihydroxivitamin D3. Purpose: This research was aimed to determine the role of 1,25- dihydroxyvitamin D3 in vivo in the development of teeth and alveolar bone tissue by analyzing MSX2 gene expression as a gene marker responsible for the growth and development of enamel, dentin, tooth root and alveolar bone. Methods: Samples used for RT-PCR analysis were total RNA of insisivus teeth and alveolar bone derived from mice. RT-PCR analysis was conducted by using primer-specific gene, MSX2. Primer gene, GAPDH, was also used as an internal control. Five hundred nanograms of total RNA were used as a template for PCR. Semi quantitative results of PCR were quantified by using ImageJ software. Results: RT-PCR analysis showed that the expression level of MSX2 was enhanced in the samples of teeth and alveolar bone treated with 1,25 dihydroxyvitamin D3. The increasing of MSX2 expression significantly occurred in alveolar bone samples. Conclusion: It can be concluded that 1,25 dihydroxyvitamin D3 could enhance MSX2 expression as a marker of the development of teeth and alveolar bone tissue. Therefore, 1,25-D3 dihydroxyvitamin is expected to be used as an agent to help the regeneration of teeth and bone tissue.
The effect of silanated and impregnated fiber on the tensile strength of E-glass fiber reinforced composite retainer Rosyida, Niswati Fathmah; Sunarintyas, Siti; Pudyani, Pinandi Sri
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p22-25

Abstract

Background: Fiber reinforced composite (FRC) is can be used in dentistry as an orthodontic retainer. FRC  still has a limitations because of to  a weak bonding between fibers and matrix. Purpose: This research was aimed to evaluate the effect of silane as coupling agent and fiber impregnation on the tensile strength of E-glass FRC. Methods: The samples of this research were classified into two groups each of which consisted of three subgroups, namely the impregnated fiber group (original, 1x addition of silane, 2x addition of silane) and the non-impregnated fiber group (original, 1x addition of silane, 2x addition of silane). The tensile strength was measured by a universal testing machine. The averages of the tensile strength in all groups then were compared by using Kruskal Wallis and Mann Whitney post hoc tests. Results: The averages of the tensile strength (MPa) in the impregnated fiber group can be known as follow; original impregnated fiber (26.60±0.51), 1x addition of silane (43.38±4.42), and 2x addition of silane (36.22±7.23). The averages of tensile strength (MPa) in the non-impregnated fiber group can also be known as follow; original non-impregnated fiber (29.38±1.08), 1x addition of silane (29.38±1.08), 2x addition of silane (12.48±2.37). Kruskal Wallis test showed that there was a significant difference between the impregnated fiber group and the non-impregnated fiber group (p<0.05). Based on the results of post hoc test, it is also known that the addition of silane in the impregnated fiber group had a significant effect on the increasing of the tensile strength of E-glass FRC (p<0.05), while the addition of silane in the non-impregnated fiber group had a significant effect on the decreasing of the tensile strength of E-glass FRC. Conclusion: It can be concluded that the addition of silane in the non-silanated fiber group can increase the tensile strength of E-glass FRC, but the addition of silane in the silanated fiber group can decrease the tensile strength of E-glass FRC. It is also known that the impregnation of fiber can increase the tensile strength of E-glass FRC.
The effects of ultrasonic scaling duration and replication on caspase-3 expression of Sprague Dawley rats pulp cells Nuryanti, Archadian; Soesatyo, Marsetyawan HNE; Agustina, Dewi; Sunarintyas, Siti
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p48-52

Abstract

Background: Ultrasonic scaling has been used commonly for stain and calculus removal in dental clinic for over 60years. Previous researches even had proved that ultrasonic scaling may give effects on the surface of tooth root. Ultrasonic wave exposure for 20 seconds or more can increase caspase-3 activity as an indicator of increased apoptotic cells associated with tissue damage. Purpose: This research was aimed to investigate the effects of ultrasonic scaling duration and replication on caspace-3 expression in dental pulp cells. Methods: The samples of this research were 54 male Sprague Dawley rats aged 2 months old divided into 2 groups, each of which consisted of 27 mice. The first group was induced with stain, while the second group was not. Each group was divided into 3 subgroups for ultrasonic scaling 1, 3, and 5 times. Each subgroup was divided into 3 sub-subgroups for duration procedure of 15, 30 and 60 seconds respectively. During scaling process, those rats were anesthetized using 0.1 ml of ketamine and 0.1 ml of xylol added to 2 ml of distilled water injected intramuscularly into their right thigh as much as 0.4 ml. Scaling was done on buccal surface of right first maxillary molar from cervical to occlusal. The teeth were decalcified and embedded in paraffin, then their sagittal plane was cut for thickness of 3µm and painted with immunohystochemistry for detecting caspace-3 expression of cell within dental pulp. Results: The results showed that the duration and replication of ultrasonic scaling procedures affected on the expression of caspace-3 cells as analyzed with Univariate Analisis of Variance test (p<0.05). Conclusion: It can be concluded that duration and replication of ultrasonic scaling procedure on teeth with and without stain enhauced the expression of  caspace-3 in dental pulp cells.
The effect of nanochitosan hydrogel membrane on absorbtion of nickel, inhibition of Streptococcus mutans and Candida albicans Triawan, Andi; Pudyani, Pinandi Sri; HNE, Soesatyo Marsetyawan; -, Sismindari
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p26-30

Abstract

Background: The use of fixed orthodontic appliance for a long time can potentially cause nickel ion release, increase in the growth of Streptococcus mutans (S. mutans) and Candida albicans (C. albicans). Chitosan has the ability to bind metal, antibacterial and antifungal. Physical modification of chitosan into the nanoparticles size will expand the surface of the chitosan so that the absorption of nickel ions and the inhibition of growth bacteria and fungi can be increased. Purpose: The purpose of the study was to determine the effect of nanochitosan hydrogel membrane to the absorption of nickel ions, and the inhibition of S. mutans and C. albicans growth. Methods: Nanochitosan hydrogel membrane with chitosan weight variation of 0.6; 0.8 and 1 g immersed in artificial saliva containing nickel 0.075 mg / L for 15, 30 and 45 minutes. The nanochitosan hydrogel membrane was tested for nickel ion absorption by Atomic Absorption Spectrophotometry, whereas the antibacterial and antifungal tests were done by exposing the nanochitosan hydrogel membrane - nickel on S. mutans and C. albicans in the wells of plate. Results: Demonstrated that absorption of nickel ions was related with the increase in weight of chitosan and soaking time. Inhibition of growth of S. mutans and C. albicans showed a positive correlation with the increase in weight of chitosan. Conclusion: Variation on chitosan weight on hydrogel membrane and variation on immersion time have effect on nickel ion absorption, inhibition of S. mutans and C. albicans growth.
Effect of casein phosphopeptide-amorphous calcium phosphate on the flexural strength of enamel-dentin complex following extracoronal bleaching Ratih, Diatri Nari; Pribadi, Hendargo Agung
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p7-11

Abstract

Background: Bleaching can affect the mechanical properties of enamel-dentin complex, such as flexural strength. Casein phosphopeptide-amorphus calcium phosphate (CPP-ACP) is often used following bleaching treatment to reduce hypersensitivity and to increase demineralization of tooth. Purpose: The purpose of the study was to investigate the effect of CPP-ACP on the flexural strength of enamel-dentin complex following extracoronal bleaching. Methods: Forty-eight enamel-dentin plates (size 8 x 2 x 3 mm) were randomly assigned into 6 groups, each consisted of 8 samples. Group 1, no bleaching and immersed in artificial saliva. Group 2, no bleaching, CPP-ACP application only. Group 3, bleaching using 15% carbamide peroxide. Group 4, similar to group 3, except application of CPP-ACP for the times between bleaching. Group 5, bleaching with 40% hydrogen peroxide. Group 6, similar to group 5, except application of CPP-ACP for the times between bleaching. Flexural strength of each enamel-dentin plate was tested by threepoint bending test using universal testing machine. Results: The results showed that 15% carbamide peroxide and 40% hydrogen peroxide significantly reduced flexural strength of enamel-dentin (216.25±26.44 MPa and 206.67±32.07 MPa respectively). Conversely, application of CPP-ACP following both bleachings increased flexural strength (266.75± 28.27MPa and 254.58±36.59 MPa respectively). A two-way Anova revealed that extracoronal bleaching agents significantly reduced flexural strength (p<0.05). Conclusion: Extracoronal bleaching agents reduce flexural strength, whereas application of CPP-ACP following bleaching either with 15% carbamide peroxide or 40% hydrogen peroxide can increase the flexural strength of enamel-dentin complex.
The increasing of beta-defensin-2 level in saliva after probiotic Lactobacillus reuteri administration Kusumaningsih, Tuti
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p31-34

Abstract

Background: Commesal bacteria is an excellent inducer for beta defensin-2 (BD-2). Probiotics bacteria Lactobacillus reuteri (L. reuteri) as commensal bacteria may play the same role as an excellent inducer for BD-2. Beta defensin is natural antimicrobial peptides widely expressed in oral cavity, including in epithelium salivary gland. Streptococcus mutans (S. mutans) as the main of bacteria causing caries are sensitive to BD-2. Purpose: This research was aimed to determine whether administration of probiotic L. reuteri can increase salivary BD-2 level in Wistar rats. Methods: This research can be considered as a laboratory experimental research with a randomized control group post test only design. Twenty-four male Rattus norvegicus Wistar strain rats aged 3 months were used. They were randomly divided into four groups, namely two control groups (negative control group that was not induced and positive control group induced with S. mutans), and two treatment groups (K1: induced with L. reuteri for 14 days and S. mutans for 7 days, and K2: induced with L. reuteri and S. mutans simultaneously for 14 days). L. reuteri culture at a concentration of 108 CFU/ml and S. mutans culture at a concentration of 1010CFU/ml were induced into the oral cavity of Wistar rats. An examination of BD-2 level was then conducted by using Elisa techniques. results: There was significant difference of salivary BD-2 level among those treatment groups (p=0.001). BD-2 level in saliva was increased after the administration of L. reuteri. Conclusion: L. reuteri probiotic can increase salivary BD-2 level in Wistar rats.
Trans-adapted, reliability, and validity of children fear survey schedule-dental subscale in Bahasa Indonesia Suzy, Arlette; Amriwijaya, Julian; Fitriana, Efi
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p1-6

Abstract

Background: The most frequently used measuring instrument for determination of dental fear in children nowadays is the children’s fear survey schedule-dental scale (CFSS-DS). Purpose: The purpose of this study was to explore the reliability and validity of the scale with Indonesian trans adapted version of the scale, thus the scale can be reliable to be used in other similar research in Indonesia. Methods: Total of 113 participants, who were parent’s 3 to 12 years old children. Children were divided into two age groups, group I 3-6 year old (83 children) and group II 7-12 year old (30 children). Eighty three children from the first group were divided into first dental visit group (30 children) and non first dental visit group (53 children). Test-retest approach was applied to 30 first dental visit children aged 3-6 year old. Original scale was translated to Indonesian language. Result: The result showed the high value of the Cronbach’s coefficient of internal consistency α=0.956. Three factors were extracted by screen test method with Eigen values higher than 1, which explained 93.05% variance of results. Conclusion: CFSS-DS scale is reliable and valid psychometric instrument for dental fear evaluation in children in Bahasa Indonesia. The differences between this study and those of others may appear due to many factors.
The decreasing of NFκB level in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with application of 1% curcumin on gingival sulcus Krismariono, Agung
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p35-38

Abstract

Background: Periodontal disease is a chronic, multi-factorial disease. Chronic periodontitis is one of the main causes of tooth loss. Chronic periodontitis is usually caused by Porphyromonas gingivalis (P. gingivalis). P. gingivalis can induce NFκB activation resulting in the increasing of periodontal extracellular matrix degradation. Curcumin can inhibit NFκB activation and reduce the severity of periodontal degradation. Purpose: This research was aimed to observe level of NFκB in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with local administration of curcumin. Methods: Sixteen Wistar rat were divided into two groups. Group 1 (treatment) consisted of eight rat given 2 x 106CFU/ml P. gingivalis and 1% curcumin. Meanwhile, group 2 (control) consisted of eight rat given 2 x 106 CFU/ml P. gingivalis only. GCF samples were collected from gingival sulcus. The samples were biochemically analyzed with ELISA method. Data were then analyzed statistically by using independent t-test (α=0.05). Results: The examination of NFκB level showed that there was significant difference between treatment group and control group (p<0.05). The level of NFκB in the treatment group was significantly lower than the control group. Conclusion: It can be concluded that 1% curcumin application can reduce NFκB level in gingival junctional epithelium of rat exposed to P. gingivalis.
The application of lesion sterilization and tissue repair 3Mix-MP for treating rats dental pulp tissue Nugroho, Raditya; Budi, Ananta Tantri; Kunarti, Sri
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p12-15

Abstract

Background: Lesion sterilization and tissue repair (LSTR) 3Mix-MP are three broad-spectrum antibiotics, including metronidazole, ciprofloxacin and minocycline are mixed with propylene glycol or macrogol. There is the possibility ofthe healing process that marked proliferation ofnew blood vessels and proliferation offibroblasts in the treatment ofirreversible pulpitis by pulp capping LSTR 3MixMP because of  the principle of the method LSTR 3Mix-MP is to kill bacteria. Purpose: The purpose of this study to prove the effect of LSTR 3Mix-MP on chronic inflammation and the healing process in rat dental pulp tissue in vivo. Methods: Rattus norvegicus anaesthetized by using ketamine and xylazine dissolved in sterile isotonic saline solution (0.2 ml/50gr mm) on the upper right thigh. Cavity preparation class I to perforation by using a low speed tapered diamond round bur. In the treatment group, rats were treated 3Mix-MP at a dose of10 mg and then covered with glass ionomer cement for 7 days on the pulp that has been opened for 3 days. The control group treated with saline irrigation on the pulp that has been opened for 3 days. Rats were killed after seven days, and then made preparations pulp tissue to count the number oflymphocytes, macrophages, plasma cells, blood vessels, and fibroblasts Results: There is an increase in the average number ofmacrophage cells, plasma, and fibroblasts; and decreased lymphocytes and blood vessels in the treated group exposure LSTR 3Mix-MP. Conclusion:LSTR 3Mix-MP can reduce chronic inflammation process and enhance the healing process in rat dental pulp tissue.
The influence of adhesin protein from Aggregatibacter actinomycetemcomitans on IL-8 and MMP-8 titre in aggressive periodontitis Ridwan, Rini Revijanti
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 1 (2015): (March 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v48.i1.p39-42

Abstract

Background: Adhesion can actually be considered as a part of both a powerful survival mechanism and a virulence mechanism for bacterial pathogens. Bacterial adhesin is an instrument for bacteria to do invasion to host. Bacterial adhesin depends on ligand interaction as a signaling mediator that will influence invasion and increase pro and anti-inflammatory because of the influence of the receptors of innate immune response. Aggregatibacter actimycetemcomitans has fimbriae included in type IV pili containing mostly with protein weighed 6.5 kDa and at least with protein weighed 54 kDa. Purpose: The purpose of this research is to analyze the influence of the induction of adhesin protein derived from A. actinomycetemcomitans on IL-8 and MMP-8 titre of Wistar rats. Methods: Adhesin protein derived from A. actinomycetemcomitans weighed 24 kDa was induced on the maxillary first molar sulcus of Wistar rats to prove that adhesin protein could affect IL-8 and MMP-8 titre. Next, to determine its influence, Elisa technique was conducted. Results: It is known that the levels of IL-8 and MMP-8 titre were increased in the group induced with adhesin protein derived from A. actinomycetemcomitans compared with the control group. Conclusion: It can be concluded that adhesin protein derived from A. actinomycetemcomitans can cause alveolar bone damage through the increasing levels of IL-8 and MMP-8 in aggressive periodontitis.

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