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Dental Journal (Majalah Kedokteran Gigi)
Published by Universitas Airlangga
ISSN : 19783728     EISSN : 24429740     DOI : -
Core Subject : Health,
The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.
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Articles 11 Documents
Search results for , issue " Vol 47, No 3 (2014): (September 2014)" : 11 Documents clear
Pengaruh lama pemberian aspirin pada ekspresi protein KI-67 dan ketebalan epitel mukosa rongga mulut tikus Wistar jantan (The effect of aspirin administration period on KI-67 expression protein and oral epithelial mucosal thickness in male Wistar mice) Arinawati, Dian Yosi; Susilowati, Heni; Supriatno, Supriatno
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p135-140

Abstract

Background: Aspirin has been widely used as an anti-inflammatory, antipyretic, and analgesics drugs. It has seriously side effects like gastrointestinal ulceration, delayed healing ulcer, and oral mucosal ulceration when Aspirin is administered for long time. Purpose: The aim of study was to examine the effect of Aspirin administration period on Kiehl-67 (KI-67) protein expressions and oral mucosal epithelial thickness in male Wistar mice. Methods: Experimental laboratory study with post-test only control group design was performed and 40 male Wistar mice were used in this experiment. The samples were divided into 2 groups. Group I was treated with Aspirin, whereas group II was receive aquadest. Each group was divided into 5 subgroups for assessment of the length administration effect. All of mice were sacrificed on day 1, 3, 5, 7 and 10 after treatment. Aspirin was orally administrated with doses of 9 mg/kg body weight. The buccal right of mice oral mucosal tissue was sliced and delivered for immunohistochemistry staining using anti-KI-67. Hematoxylin-eosin (HE) staining was performed to measure the oral epithelial thickness. Examination of KI-67 expressions and oral epithelial thickness were performed by using ImageJ software. Two-way Anova and Kruskall-Wallis test were carried-out for data analysis with significant level of 95%. Results: The results revealed that the administration of Aspirin in mice on day 1, 3, 5, 7, and 10 was markedly decreased in the KI-67 protein expressions and oral epithelial thickness compared with that of control (p<0.05), otherwise the duration of Aspirin administration did not affect mucosal epithelial thickness. Conclusion: Aspirin administration period has the potential to suppress the KI-67 protein expression within 10 days; the effect in line with the length of duration. The epithelial thickness was not influenced by the length of Aspirin administration.Latar belakang: Aspirin digunakan sebagai anti inflamasi, anti demam, dan anti nyeri. Aspirin merupakan obat yang aman, namun dilaporkan menimbulkan efek samping berupa kerusakan gastrointestinal dan kerusakan mukosa rongga mulut apabila dikonsumsi dalam jangka panjang. Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh lama pemberian Aspirin terhadap ekspresi KI-67 dan ketebalan epitel rongga mulut tikus galur Wistar. Metode: Jenis penelitian eksperimental laboratories dan menggunakan 40 tikus jantan galur Wistar. Hewan coba dibagi menjadi 2 kelompok, yaitu kelompok perlakuan Aspirin dan kontrol akuades. Masing-masing kelompok dibagi menjadi 5 subkelompok berdasarkan lama pemberian Aspirin, yaitu 1, 3, 5, 7 dan 10 hari. Dosis yang diberikan 9 mg/kg berat badan sekali per hari. Mukosa bukal tikus kemudian dipotong untuk pengecatan KI-67 dan hematoxylin-eosin (HE). Ekspresi KI-67 dan ketebalan epitel diukur menggunakan software ImageJ. Data dianalisis menggunakan Two-way Anova and KruskallWallis dengan tingkat kepercayaan 95%. Hasil: Lama pemberian Aspirin hari ke 1, 3, 5, 7 dan 10 dapat menurunkan ekspresi KI-67 (p<0.05) dan ketebalan epitel dibandingkan kontrol, namun lama pemberian tidak berpengaruh terhadap mukosa epitel. Aspirin dapat menurunkan ketebalan epitel rongga mulut dibandingkan kontrol (p<0.05). Simpulan: Pemberian Aspirin dapat menurunkan ekspresi KI-67 pada sel epitel mukosa rongga mulut tikus galur Wistar; efek tersebut berbanding lurus dengan durasi pemberian sampai hari ke-10. Lama pemberian Aspirin tidak berpengaruh terhadap ketebalan mukosa rongga mulut tikus galur Wistar.
Pelepasan ion nikel dan kromium kawat Australia dan stainless steel dalam saliva buatan (The release of nickel and chromium ions from Australian wire and stainless steel in artificial saliva) Rasyid, Nolista Indah; Pudyani, Pinandi Sri; Heryumani, JCP
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p168-172

Abstract

Background: Fixed orthodontic treatment needs several types of wire to produce biomechanical force to move teeth. The use orthodontic wire within the mouth interacts with saliva, causing the release of nickel and chromium ions. Purpose: The study was aimed to examine the effect of immersion time in artificial saliva between special type of Australian wire and stainless steel on the release of nickel and chromium ions. Methods: Thirty special type Australian wires and 30 stainless steel wires were used in this study, each of which weighed 0.12 grams. The wires were immersed for 1, 7, 28, 35, 42, and 49 days in artificial saliva with a normal pH. The release of ions in saliva was examined using Atomic Absorption spectrophotometry. Results: The result indicated that the release of nickel ions on special type of Australian wire was larger than that on stainless steel wire (p<0.005), there were differences in the release of the amount of nickel ions on special type of Australia in different immersion time, and there was a correlation between the types of wire and immersion time. Nickel ions released from the special type of Australian wire detected on the 7th day of immersion and reached its peak on the 35th day, while from stainless steel wire were detected on the 49th day of immersion. The released of chromium ions from the special type of Australian wire and stainless steel wire were not detected until the 49th day of immersion. Conclusion: The release of nickel ions were highest on the 35th day of immersion in special type of Australian wire and they were detected on the 49th day in stainless steel wire. The release of chromium ions were not detected until 49th day of immersion in special type of Australian and stainless steel wire.Latar belakang: Perawatan ortodonti cekat memerlukan beberapa macam kawat untuk menghasilkan kekuatan biomekanika yang sesuai dalam menggerakkan gigi. Pemakaian kawat ortodonti di dalam mulut dapat bereaksi dengan saliva sehingga menyebabkan terjadinya pelepasan ion nikel dan kromium. Tujuan: Penelitian ini bertujuan untuk meneliti pengaruh waktu perendaman dalam saliva buatan terhadap pelepasan ion nikel dan kromium antara kawat Australia tipe spesial dengan kawat stainless steel. Metode: Penelitian ini menggunakan 30 buah kawat Australia tipe spesial dan 30 buah kawat stainless steel dengan berat tiap kawat 0,12 gram. Kawat direndam selama 1, 7, 28, 35, 42 dan 49 hari dalam saliva buatan pH normal. Pemeriksaan pelepasan ion pada saliva menggunakan spektrofotometri serapan Atom. Hasil: Pelepasan ion nikel pada kawat Australia tipe spesial lebih besar dibandingkan dengan kawat stainless steel, terdapat perbedaan pelepasan jumlah ion nikel pada kawat Australia tipe spesial antara waktu perendaman dan terdapat interaksi antara jenis kawat dengan waktu perendaman. Ion nikel yang terlepas pada kawat Australia tipe spesial mulai terdeteksi pada hari ke-7 perendaman dan mencapai jumlah tertinggi pada hari ke-35 sedangkan pada kawat stainless steel mulai terdeteksi pada perendaman hari ke-49. Pelepasan ion kromium pada kawat Australia tipe spesial dan kawat stainless steel tidak terdeteksi sampai perendaman hari ke-49. Simpulan: Pelepasan ion nikel pada kawat Australia tipe special terdeteksi paling tinggi pada lama perendaman hari ke tiga puluh lima, kawat stainless steel terdeteksi pada lama perendaman hari ke empat puluh sembilan. Pelepasan ion kromium pada kawat Australia tipe special dan stainless steel tidak terdeteksi sampai lama perendaman hari ke empat puluh sembilan.
Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient Wati, Sisca Meida; Istiati, Istiati; Soesilawati, Pratiwi
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p141-145

Abstract

Background: Human periodontal diseases are inflammatory disorders as the result of complex interactions between periodontopathogens and the host’s immune response. Periodontitis results in tooth loss and can even lead to systemic diseases if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamated sites. Polymorphonuclear leukocyte (PMNs) infiltration can be seen in each stage of periodontitis. Lactoferrin is one of the PMN specific granules and could be a useful marker of PMN activity. Purpose: The aim of this study was to determine the band intensity of lactoferrin used as periodontitis biomarker. Methods: Gingival crevicular fluid (GCF) samples were collected using paper point no.30 from 40 subjects, 30 periodontitis patients that devide according to the severity (10 mild periodontitis, 10 moderate periodontitis, and 10 severe periodontitis) and 10 healthy controls, ranging in ages from 20 to 35 years. GCF lactoferrin was analyzed by Western blot and measured the band intensity by quantity one software (bio-rad). Results: The periodontitis sites exhibited significantly greater band intensity of lactoferrin than healthy sites. The band intensity of lactoferrin was positively correlated with the severity of periodontitis (α = 0.05). Conclusion: The study showed that the intensity of the lactoferrin protein bands could be used as biomarkers of periodontitis.Latar belakang: Penyakit periodontal adalah gangguan inflamasi yang merupakan hasil dari interaksi yang kompleks antara periodontopathogens dan respon imun host. Periodontitis mengakibatkan hilangnya gigi dan bahkan dapat menyebabkan penyakit sistemik jika tidak diobati. Cairan sulkus gingiva (GCF) mencerminkan kondisi gingiva dan mengandung protein yang tertransudasi dari serum atau sel pada lokasi radang. Infiltrasi polymorphonuclear leukosit (PMN) dapat dilihat pada setiap tahap periodontitis. Laktoferin adalah salah satu granula spesifik PMN dan bisa menjadi indicator aktivitas PMN. Tujuan: Tujuan penelitian ini adalah untuk meneliti intensitas band laktoferin dapat sebagai biomarker periodontitis. Metode: Cairan sulkus gingiva (GCF) dari tiap sampel dikumpulkan menggunakan paper pint no. 30 dari 40 subjek, 30 pasien dengan periodontitis yang dibagi sesuai dengan tingkat keparahan (10 periodontitis ringan, 10 periodontitis moderat, dan 10 periodontitis parah) dan 10 kontrol, mulai usia 20-35 tahun. GCF lactoferrin dianalisis dengan Western blot dan diukur intensitas bandnya dengan quantity one software (bio-rad). Hasil: Pada jaringan yang mengalami periodontitis menunjukkan intensitas band yang secara signifikan lebih besar dari laktoferin daripada periodontal yang sehat. Intensitas band laktoferin berkorelasi positif dengan tingkat keparahan periodontitis (α = 0,05) Simpulan: Hasil penelitian ini menyimpulkan bahwa intensitas band protein laktoferin dapat digunakan sebagai biomarker periodontitis.
Perubahan suhu transisi kaca dan massa resin akrilik heat cured akibat kelembaban dan lama penyimpanan (Changes in glass transition temperature and heat cured acrylic resin mass due to moisture and storage time) Salim, Sherman
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p173-177

Abstract

Background: Acrylic resins, especially poly methyl methacrylate (PMMA) was introduced in 1937. Acrylic resin has favorable properties, among others, aesthetic, color and texture similar to that of the gingival aesthetic in the mouth, relatively low water absorption and dimensional changes. However, some studies suggest that the duration of storage of acrylic resin will affect the changes in the glass transition temperature and the mass of acrylic resin. Purpose: The objective of this research was to study the effect of humidity and storage time led to changes in the glass transition temperature and the mass of the acrylic resin. Methods: The research method is experimental laboratory. Acrylic resin specimens are kept in conditions of humidity of 90%, 70%, 40% and 30% for 24 hours, one week, one month and two months. In this study used three methods of curing, namely conventional JIs, 24-hour curing at 70 °C and using the microwave. Results: Low humidity causes changes in the glass transition temperature and the mass of acrylic resin. Longer storage of acrylic resins in low humidity, can affect change greater than the glass transition temperature and the mass of acrylic resin. Conclusion: It can be concluded that the humidity and longer storage of acrylic resins can affect the glass transition temperature and a change in mass.Latar belakang: Resin akrilik terutama poli metil metakrilat (PMMA) telah diperkenalkan pada tahun 1937. Resin akrilik memiliki sifat yang menguntungkan antara lain estetis, warna dan tekstur mirip dengan gingiva sehingga estetik di dalam mulut baik, daya serap air relatif rendah dan perubahan dimensi kecil. Akan tetapi, dari beberapa penelitian menyatakan bahwa lamanya waktu penyimpanan resin akrilik akan berpengaruh pada perubahan suhu transisi kaca dan massa resin akrilik. Tujuan: Tujuan dari penelitian ini adalah untuk mempelajari pengaruh kelembaban dan waktu penyimpanan yang menyebabkan perubahan suhu transisi kaca dan massa pada resin akrilik. Metode: Metode penelitian adalah eksperimen laboratoris. spesimen resin akrilik disimpan dalam kondisi kelembaban 90%, 70%, 40% dan 30% selama 24 jam, satu minggu, satu bulan dan dua bulan. Dalam penelitian ini digunakan tiga metode curing, yaitu konvensional JIs, 24 jam curing pada suhu 70 °C dan menggunakan microwave. Hasil: Kelembaban rendah menyebabkan perubahan suhu transisi kaca dan massa resin akrilik. Penyimpanan lebih lama dari resin akrilik dalam kelembaban rendah, dapat mempengaruhi perubahan yang lebih besar dari suhu transisi kaca dan massa dari resin akrilik. Simpulan: Dapat disimpulkan bahwa kelembaban dan penyimpanan yang lebih lama dari resin akrilik dapat mempengaruhi suhu transisi kaca dan perubahan massa.
Oral health knowledge among parents of autistic child in Bandung-Indonesia Nonong, Yetty Herdiyati; Setiawan, Arlette; Dewi, Fellani Danasra; Navaneetha, Cugati
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p146-152

Abstract

Background: Autistic children as well as other special needs individual demand special care given by their parents. But there exist limited awareness among parents in Indonesia society, especially with regard to their oral health. Purpose: The study was aimed to assess the oral health related knowledge, attitude and behavior of the parents; and oral health status of their autistic children in comparison with non-autistic children. Methods: Total of 56 children (23 autistic and 23 normal) between 7-12 years was included in this study. Data on parents’ knowledge, attitude, oral health practice and behavior of their children were gathered from the questionnaires. The oral health status of the children was recorded using deft and DMFT caries index. Results: All obtained data were analyzed using sPss version 13 to correlate the index of the sample. It showed that caries index of autistic child was lower and limited oral health knowledge among parents. Conclusion: There is need of greater awareness to be spread among the population of Indonesia about the existing professional help for the special children and educate the parents to maintain their child’s oral health for a better quality of life.Latar belakang: Anak autis seperti juga individu berkebutuhan khusus lainnya memerlukan perhatian khusus dari orang tuanya. Namun banyak keterbatasan kesadaran orang tua dalam masyarakat Indonesia, terutama berkaitan dengan kesehatan mulut anak autis mereka. Tujuan: Penelitian ini bertujuan untuk menguji pengetahuan kesehatan gigi, perilaku orang tua dan anak mereka yang autis. Metode: Data pengetahuan orang tua dikumpulkan dari kuesioner dan status kesehatan mulut anak dicatat menggunakan indeks karies DMFT dan deft. sejumlah 56 anak (23 autis dan 23 non-autis sebagai kelompok kontrol) usia 7-12 tahun ikut serta dalam penelitian ini. Hasil: Data yang didapatkan dianalisis menggunakan sPss versi 13 untuk mengkorelasikan indeks subjek. Hasil menunjukkan indeks karies anak autis lebih rendah dengan pengetahuan kesehatan mulut orang tua yang terbatas. Simpulan: Diperlukan penyebarluasan kesadaran yang lebih tinggi di antara populasi orang Indonesia mengenai mempertahankan status kesehatan mulut anak autis mereka untuk mencapai kualitas hidup yang lebih baik.
Pengaruh chitosan belangkas (Tachypleus gigas) nanopartikel terhadap celah antara berbagai jenis semen ionomer kaca dengan dentin Sutrisman, Henny; Abidin, Trimurni; Agusnar, Harry
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p121-125

Abstract

Background: The development of dental material restoration is regarded to be relevant to obtain a better bonding between dental structure and restorative materials. Glass ionomer cement (GIC) is a bioactive material. Resin-modified GIC (RMGIC) is an alternative to the conventional glass ionomer. Nowadays with nano technology, this material is available in nano particle glass ionomer form in order to enhance the bond strength between tooth structure and restoration. The use of the natural product in dentistry such as chitosan has increased widely. Chitosan is one of the natural materials that used to improve the bioactivity of the glass ionomer. studies showed that addition of chitosan high molecule to GIC can increase mechanical performance and capability and also as a catalyst to release fluoride ions. Purpose: This study was aimed to examine the effect of the addition of high molecular nanoparticle chitosan derived from horseshoe crab (Tachypleus gigas) on interface of RMGIC, nano RMGIC and the dentin surface. Methods: Nano particle chitosan was added to the restorative materials and then applied to the class I cavity of premolar and then the tooth was sectioned with diamond disc. specimens were prepared for sEM examination. Results: The result showed that the addition of chitosan increases adhesion between restoration and dentin structure. Conclusion: The addition of nanoparticle chitosan with a high molecular weight of 0.015% into RMGIC and nanoparticle RMGIC can improve the adhesion of restorative material to dentin structure.Latar belakang: Perkembangan restorasi bahan gigi untuk mendapatkan ikatan yang lebih baik antara struktur gigi dan bahan restoratif. semen ionomer kaca (sIK) adalah bahan bioaktif. semen ionomer kaca modifikasi resin (sIKMR) adalah sebuah alternatif untuk ionomer kaca konvensional. saat ini dengan teknologi nano, bahan ini tersedia dalam bentuk partikel nano ionomer kaca untuk meningkatkan kekuatan ikatan antara struktur gigi dan restorasi. Penggunaan produk alami dalam kedokteran gigi seperti kitosan telah meningkat banyak. Kitosan merupakan salah satu bahan alami yang digunakan untuk meningkatkan bioaktivitas dari ionomer kaca. studi menunjukkan bahwa penambahan molekul tinggi kitosan untuk GIC dapat meningkatkan kinerja mekanik dan kemampuan dan juga sebagai katalis untuk melepaskan ion fluoride. Tujuan: Penelitian ini bertujuan untuk menguji pengaruh penambahan kitosan nanopartikel molekul yang berasal dari kepiting tapal kuda (Tachypleus gigas) terhadap permukaan antara RMGIC, nano RMGIC dan permukaan dentin. Metode: Nano partikel kitosan ditambahkan pada bahan restoratif dan kemudian diterapkan pada kavitas kelas I premolar dan kemudian gigi tersebut dipotong dengan disc berlian. sampel disiapkan untuk pemeriksaan sEM. Hasil: Hasil penelitian menunjukkan bahwa penambahan kitosan meningkatkan adhesi antara restorasi dan struktur dentin. Simpulan: Penambahan nanopartikel kitosan dengan berat molekul tinggi 0,015% pada sIKMR dan sIKMR nano dapat meningkatkan adhesi bahan restorasi struktur dentin.
Efektifitas siwak (Salvadora persica) dan pasta gigi siwak terhadap akumulasi plak gigi pada anak-anak (Effectiveness of Siwak (Salvadora persica) and siwak toothpaste on dental plaque accumulation in children) Bramanti, Indra; RS, Iwa Sutardjo; Ula, Navilatul; Isa, Muhammad
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p153-157

Abstract

Background: siwak contain of salvadorine with an antiseptic effect. There were many reports about antibacterial effect of siwak on the cariogenic bacterial, pathogen periodontal, and dental plaque accumulation. Purpose: The study was aimed to determine the effect of siwak and siwak toothpaste on accumulated dental plaque in children. Methods: The subjects were 39 teenage children in range of age 12-15 years old, and were divided on 3 groups. Each subject group was asked to brush their teeth 3 times a day using siwak; siwak toothpaste; and toothpaste with no additional substance as control, respectively. After brushing for a week, plaque scoring was performing using Modified Personal Hygiene Performance Index (PHP-M). Data were analysed using one way Anova. Results: The plaque score on siwak group lower significantly than control group, but there was no significant difference between siwak group and siwak toothpaste group. Conclusion: The study suggested that siwak and siwak toothpaste had the same effect on decreasing plaque accumulation in children.Latar belakang: siwak mengandung salvadorine yang berefek sebagai antiseptik. siwak juga dilaporkan memiliki efek antibakteri terhadap bakteri kariogenik dan pathogen periodontal, dan menghambat pembentukan plak. Tujuan: Penelitian ini bertujuan untuk menguji efek siwak dan pasta gigi siwak terhadap akumulasi plak pada anak. Metode: subyek penelitian adalah 39 anak remaja berusia 12-15 tahun yang dibagi dalam 3 kelompok. setiap subyek dalam kelompok yang sama diminta untuk menyikat gigi sehari 3 kali dengan menggunakan siwak; pasta gigi siwak; dan pasta gigi murni tanpa tambahan bahan sebagai control. setelah selama seminggu menyikat gigi, dilakukan pengukuran skor plak menggunakan indeks PHP-M. Data yang diperoleh dianalisis menggunakan Anova satu jalur. Hasil: skor plak kelompok siwak lebih rendah secara signifikan dibanding kelompok pasta gigi murni, namun antara kelompok siwak dan pasta gigi siwak tidak terdapat perbedaan yang bermakna. Simpulan: studi ini menunjukkan bahwa siwak dan pasta gigi siwak memiliki efek yang sama dalam menurunkan akumulasi plak pada anak.
Perbandingan sitotoksisitas tiga jenis algyrogel terhadap sel fibroblas (Citotoxicity comparation of three types of algyrogel on fibroblast cells) Saktiyawardani, Stefany Elan; Lauson, Hardono Jaya; Astamurtiningrum, Anugerah Pekerti; Rahmah, Mahadna Aulia; Putra, Pramana Pananja; Handajani, Juni
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p130-134

Abstract

Background: The prevalence of osteoporosis and bone defects in the world and Indonesia increase each year. Algyrogel is a alginate-based hydrogel material recently very popular used as a bone substitute through injectable bone substitute (IBs) method. One of advantages algyrogel is biocompatibility to the body tissues. some natural-based polymer (alginate, chitosan, dan hyaluronat) have different biocompatibility. Purpose: The study was aimed to determine the cytotoxicity of three types algyrogel against fibroblast cells. Methods: This study used three types algyrogel, there were algyrogel containing alginate (Alg), a mixture of alginate with chitosan (Alg/Ch), and a mixture of alginate with hyaluronic acid (Alg/Ha). Each type algyrogel was exposed in the Vero cell line of fibroblast with volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; and 20 μL respectively. The cytotoxicity performed by MTT method to determine the percentage of cell death. The optical density was measured by an ELIsA plate reader then data was analyzed using ANOVA and probit. Results: The percentage of cell death in all groups of algyrogel have below 30%. LC50 value at Alg was 651.017 mg/mL (21.56%), Alg/Ch was 280.478 mg/mL (20.91%), and Alg/Ha was 1054.094 mg/ mL (17.2%). Conclusion: Alg/Ha has the lowest cytotoxicity on fibroblast cells and may have potential as an osteoconductor-synthetic bone product.Latar belakang: Prevalensi osteoporosis dan cacat tulang di Indonesia maupun dunia semakin meningkat tiap tahunnya. Algyrogel sebagai bahan hidrogel yang berbasis alginat akhir-akhir ini sangat popular digunakan sebagai bahan pengganti tulang melalui metode Injectable Bone substitute. Kelebihan algyrogel antara lain biokompatibel terhadap jaringan tubuh. Beberapa hidrogel berbasis polimer alami (alginat, chitosan, dan hyaluronat) memiliki perbedaan biokompatibilitas. Tujuan: studi ini bertujuan meneliti sitotoksisitas tiga jenis algyrogel terhadap sel fibroblas. Metode: Penelitian ini menggunakan 3 jenis algyrogel yaitu algyrogel dengan kandungan alginat (Alg), campuran alginat dengan chitosan (Alg/Ch), dan campuran alginat dengan asam hyaluronat (Alg/Ha). setiap jenis algyrogel dipaparkan pada sel fibroblas jenis Vero cell line dengan volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; dan 20 μL. Pengujian sitotoksisitas dilakukan dengan metode MTT untuk mengetahui persentase kematian sel. Penghitungan optical density menggunakan ELIsA plate reader lalu data diuji Anova dan probit. Hasil: Persentase kematian sel pada semua jenis algyrogel memiliki angka dibawah 30%. Nilai LC50 pada Alg sebesar 651,017 μg/μL (21,56%), Alg/Ch sebesar 280,478 μg/μL (20.91%), dan "> Alg/Ha sebesar 1054,094 μg/μL (17,2%). Simpulan: Alg/Ha memiliki sitotoksisitas paling rendah pada sel fibroblas sehingga memiliki potensi sebagai produk osteokonduktor sintesis tulang.
Penatalaksanaan impaksi caninus permanen rahang atas dengan surgical exposure (The management of impacted permanent canine with surgical exposure) Wijaya, Syeh Brata; Utomo, Rinaldi Budi
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p158-163

Abstract

Background: Impacted tooth is often unidentified because there is no symptom. It is found when patient is examined by dentist. The maxillary canine should be retained for strength masticatory function, esthetics and child development. Purpose: The article was aimed to report treatment options of impacted canine in the 13 years old child. Case: Thirteen years-old girl came to the Universitas Gadjah Mada Dental Hospital with complaints of the upper right permanent canine had not erupted, with no history of pain. Periapical radiograph showed the impacted position of tooth #13 mesioangular. The shift sketch technique radiograph showed the impacted canine located at the palatal site. Case management: surgical exposure the upper right maxillary canine was done, followed by orthodontic treatment to direct tooth position into occlusal line. Fixed orthodontic appliance used was Roth bracket with straight wire technique. After surgery and orthodontic treatment, #13 was in normal occlusion. Conclusion: The surgical exposure followed by orthodontic treatment could be done successfully with special consideration to the patient’s age, the dental space, location of dental crowns, dental inclination, the apical root form of impacted tooth and patient cooperation.Latar belakang: Terjadinya gigi impaksi biasanya diketahui setelah melakukan pemeriksaan ke dokter gigi karena jarang menimbulkan keluhan. Gigi caninus rahang atas sebaiknya dipertahankan untuk kekuatan fungsi pengunyahan, estetik dan tumbuh kembang anak. Tujuan: Artikel ini bertujuan untuk melaporkan perawatan impaksi gigi kaninus atas pada anak 13 tahun. Kasus: Anak perempuan usia 13 tahun datang ke Rumah sakit Gigi dan Mulut Fakultas Kedokteran Gigi Universitas Gadjah Mada dengan keluhan gigi kaninus permanen kanan atas yang belum erupsi, tanpa ada riwayat sakit di area tersebut. Hasil radiografi periapikal menunjukkan posisi gigi #13 impaksi mesioangular. Hasil radiografi dengan teknik shift sketch menunjukkan gigi kaninus yang impaksi terletak di palatal. Tatalaksana kasus: Dilakukan perawatan exposure surgical pada gigi #13, dilanjutkan dengan perawatan ortodontik untuk menempatkan posisi gigi ke arah oklusal. Alat ortodontik cekat yang digunakan adalah braket Roth dengan teknik straight wire. setelah dilakukan tindakan bedah dan penarikan ortodontik, gigi #13 berada pada ruang yang telah disediakan dan sudah masuk pada posisi oklusi. Simpulan: surgical exposure yang dilanjutkan perawatan ortodontik dapat dilakukan dengan sukses dengan perhatian khusus pada usia pasien, ruang gigi, letak mahkota gigi, inklinasi gigi dan bentuk apeks akar gigi yang impaksi.
Modulation of FGF2 after topical application of Stichopus hermanii gel on traumatic ulcer in Wistar rats Sari, Rima Parwati; Wahjuningsih, Endah; Soeweondo, isidora Karsini
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 3 (2014): (September 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i3.p126-129

Abstract

Background: stichopus hermanii (golden sea cucumber) is one of the many types of marine organisms containing glycosaminoglycans (GAGs), a polysaccharide that promote wound healing. The content of this GAGs, mainly dermatan sulfate, chondroitin sulfate and heparan sulfate has the ability to modulate FGF2. FGF2 many found in the oral mucosa to activate fibroblast proliferation. Purpose: The study was aimed to determine the modulation of FGF2after topical application of stichopus hemanii gel on traumatic ulcers in wistar rat. Methods: The sample was 36 male Wistar rats which were divided into 6 groups. C1 and C2 group was placebo gel, sC1 and sC2 group was stichopus hermanii gel, HA1 and HA2 was hyaluronic acid A gel. The gel was given shortly after the traumatic ulcer (TU) formed and 24 hour later. Then all rats were sacrificed lips mucosa were taken and ELIsA examination was done. Results: All data were analyzed by ANOVA test followed by Tukey HsD. Test results show significant differences between sC1-sC2 with C1- C2 group, while the HA1-HA2 with C1-C2 group showed no significant difference. Conclusion: The study showed that modulation of FGF2 increased after topical application of stichopus hermanii gel on traumatic ulcers in wistar rats.Latar belakang: stichopus hermanii (teripang emas) merupakan salah satu jenis biota laut yang banyak mengandung GAG, suatu polisakarida yang sangat bermanfaat dalam proses penyembuhan luka. Kandungan GAG ini, terutama dermatan sulfat, chondroitin sulfat dan heparan sulfat memiliki kemampuan untuk memodulasi FGF2. FGF2 banyak didapatkan pada mukosa rongga mulut untuk mengaktifkan proliferasi fibroblas. Tujuan: studi ini bertujuan meneliti modulasi FGF2setelah pemberian aplikasi topikal gel stichopus hermanii pada ulkus traumatikus pada tikus wistar. Metode: sampel penelitian ini adalah 36 ekor tikus wistar jantan yang dibagi dalam 6 kelompok. Kelompok C1 dan C2 merupakan kelompok kontrol negatif (gel plasebo), Kelompok sC1 dan sC2 merupakan kelompok pemberian gel stichopus hermanii (sC), serta kelompok asam hyaluronat HA1 dan HA2 merupakan kelompok pemberian gel asam hialuronat (AH). Pemberian gel dilakukan sesaat setelah pembuatan ulkus traumatikus dan 24 jam kemudian. Kemudian semua tikus dikorbankan untuk diambil mukosa bibirnya dan dilakukan pemeriksaan ELIsA. Hasil: semua data dilakukan analisa dengan uji Anova dan dilanjutkan dengan uji Tukey HsD yang hasilnya menunjukkan adanya perbedaan bermakna antara kelompok sC1-sC2 dengan kelompok C1-C2, sedangkan kelompok HA1-HA2 dengan kelompok C1-C2 tidak menunjukkan perbedaan yang bermakna. Simpulan: Modulasi FGF2 meningkat setelah pemberian aplikasi topikal gel stichopus hermanii pada ulkus traumatikus tikus wistar.

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