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Dental Journal (Majalah Kedokteran Gigi)
Published by Universitas Airlangga
ISSN : 19783728     EISSN : 24429740     DOI : -
Core Subject : Health,
The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.
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Search results for , issue " Vol 47, No 1 (2014): (March 2014)" : 11 Documents clear
Uji sensitivitas dan spesifisitas perangkat lunak “Prediktor Karies Anak” (The sensitivity and specificity test of software for dental caries prediction in children) A’yun, Quroti; Hendrartini, Julita; Santoso, Al. Supartinah; Lugroho, Lukito Edi
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p45-51

Abstract

Background: The prevalence of dental caries in children is high, therefore preventive actions is needed. So far the computer preventive actions is needed. So far the computer software that have been used for caries predictor is cariogram, which determine the condition of teeth and oral mouth. Recently which determine the condition of teeth and oral mouth. Recently and oral mouth. Recently mouth. Recently“Prediktor Karies Anak” (pediatric caries predictor) software have been developed not only determine the condition of teeth and software have been developed not only determine the condition of teeth and been developed not only determine the condition of teeth and oral mouth but also child’s behavior, maternal behavior, and the environment. behavior, maternal behavior, and the environment. Purpose: The objective of this study was to examine objective of this study was to examinethe sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of "Prediktor Karies Anak" a software for dental caries prediction in children. Methods: This study study was an observational study with cross-sectional plan, carried out on 67 primary school children aged 10-12 years. The research instrument was software of “Prediktor Karies Anak” (pediatric caries of “Prediktor Karies Anak” (pediatric caries predictor) and cariogram. The data of this research was the percentage of new caries occurrence and caries risk categorized into the percentage of new caries occurrence and caries risk categorized into high and low, and analyzed with a 2 x 2 table. results: The data of 67 children was analyzed using “Prediktor Karies Anak”software and revealed 38 children had low caries risk and 29 children had high caries. The data then re-analyzed using cariogram software had low caries risk and 29 children had high caries. The data then re-analyzed using cariogram software then re-analyzed using cariogram software showed that 37 children had low caries risk, and 30 children had high caries risk. Sensitivity of “Prediktor Karies Anak” software was that 37 children had low caries risk, and 30 children had high caries risk. Sensitivity of “Prediktor Karies Anak” software was Karies Anak” software was 87%, specificity was 92%, the PPV was 90%, and NPV was 89%. Conclusion: “Prediktor Karies Anak”software had high sensitivity, Karies Anak”software had high sensitivity, high sensitivity, specificity, PPV, and NPV and could be used as an instrument to predict new caries on children.Latar belakang: Prevalensi karies pada anak tergolong tinggi, sehingga perlu dilakukan tindakan pencegahan. Selama ini karies pada anak tergolong tinggi, sehingga perlu dilakukan tindakan pencegahan. Selama ini perangkat lunak komputer yang digunakan untuk memprediksi karies gigi adalah Cariogram, yang mengukur faktor keadaan gigi yang mengukur faktor keadaan gigi dan mulut. Baru-baru ini telah dikembangkan perangkat lunak ini telah dikembangkan perangkat lunak "Prediktor Karies Anak" yang tidak hanya mengukur kondisi gigi danrongga mulut tetapi juga perilaku anak, perilaku ibu, dan lingkungan. Tujuan: Tujuan penelitian ini adalah untuk menguji sensitivitas, sensitivitas, spesifisitas, nilai duga positif (NDP) dan nilai duga negatif (NDN) perangkat lunak duga positif (NDP) dan nilai duga negatif (NDN) perangkat lunak duga negatif (NDN) perangkat lunak (NDN) perangkat lunak  "Prediktor Karies Anak" suatu perangkat lunak untuk memprediksi karies gigi pada anak. Metode: Jenis penelitian ini adalah observasional dengan rancangan cross sectional, yang dilakukan pada 67 anak sekolah dasar usia 10-12 tahun. Instrumen penelitian adalah perangkat lunak anak sekolah dasar usia 10-12 tahun. Instrumen penelitian adalah perangkat lunak dasar usia 10-12 tahun. Instrumen penelitian adalah perangkat lunak 10-12 tahun. Instrumen penelitian adalah perangkat lunak tahun. Instrumen penelitian adalah perangkat lunak "Prediktor Karies Anak" dan cariogram. Data penelitian ini berupa persentase terjadinya karies baru yang dikategorikan resiko karies tinggi dan rendah, dan terjadinya karies baru yang dikategorikan resiko karies tinggi dan rendah, dan yang dikategorikan resiko karies tinggi dan rendah, dan karies tinggi dan rendah, dan dianalisis dengan tabel 2 x 2. Hasil: Data dari 67 anak yang dianalisis dengan menggunakan perangkat lunak “Prediktor Karies Anak” menunjukkan bahwa 38 anak memiliki risiko karies rendah dan 29 anak-anak memiliki resiko karies tinggi. Data tersebut dianalisa kembali dengan perangkat lunak Cariogram, dan hasilnya menunjukkan bahwa 37 anak mempunyai resiko karies rendah, dan 30 anak anak mempunyai resiko karies rendah, dan 30 anak mempunyai resiko karies tinggi. Sensitivitas “Prediktor Karies Anak” 87%, spesifisitas 92%, NDP 90% dan NDN 89%. “Prediktor Karies Anak” 87%, spesifisitas 92%, NDP 90% dan NDN 89%. spesifisitas 92%, NDP 90% dan NDN 89%. NDP 90% dan NDN 89%. dan NDN 89%. Simpulan: “Prediktor Karies Anak” mempunyai sensitivitas, spesifitas, NDP dan NDN yang tinggi dan dapat dipakai sebagai instrumen untuk memprediksi terjadinya karies baru pada anak.
Spirulina chitosan gel induction on healing process of Cavia cobaya post extraction socket Rostiny, Rostiny; Kuntjoro, Mefina; Sitalaksmi, Ratri Maya; Salim, Sherman
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p19-24

Abstract

Background: Prominent residual ridge is necessary to gain retention and stabilility for succesful prosthodontic treatment such as removable, fixed or implant. Spirulina is a natural substance that can help tissue healing and chitosan also a natural substance that reported to have the ability to help bone remodelling. The combination gel of spirulina and chitosan could be considered as an alternative material to maintain residual ridge height after tooth extraction. Purpose: The aim of study was to examine the effect of combination gel of Spirulina and chitosan on healing process of Cavia cobaya post tooth extraction socket by counting the amount of osteoclast, osteoblast and colagen as an indicator. Methods: Twenty eight cavia cobaya were divided into 4 groups. Insisive mandible extraction was done and the sockets were filled with 3% CMCNa for control groups, 3% spirulina chitosan 200 mg for group 1, 6% spirulina chitosan 200 mg for group 2, 12% spirulina chitosan 200 mg for group 3. After 30 days, histopathology examination was done by using microscope to count the amount of osteoclast, osteoblast and collagen. Results: Data was analyzed by using Anova and Tukey HSD. For osteoclast, there was no significant different between every groups, while for osteoblast and collagen there was significant different between groups. The results showed that induction of combination gel spirulina chitosan was able to accumulate collagen fiber and resulting faster wound healing. Conclusion: Combination 12% gel spirulina chitosan 200 mg could be used as an alternative material for better bone remodeling after tooth extraction.Latar belakang: Residual ridge yang prominen sangat dibutuhkan untuk mendapatkan retensi dan stabilitas untuk menunjang keberhasilan perawatan di bidang prostodonsia seperti pada kasus removable, fixed atau implant. Tindakan pencabutan gigi dapat merusak jaringan periodontal, sementum dan tulang alveolar yang mengakibatkan resorbsi ridge yang besar. Spirulina telah terbukti mempunyai kemampuan untuk membantu penyembuhan tulang sedangkan kitosan mempunyai kemampuan untuk membantu proses pembentukan tulang. Kombinasi kedua bahan ini diharapkan dapat menjadi bahan alternatif untuk mempercepat proses penyembuhan luka dan pembentukan tulang. Tujuan: Penelitian ini bertujuan meneliti efek induksi kombinasi gel dari Spirulina dan kitosan terhadap proses penyembuhan soket pasca ekstraksi gigi Cavia cobaya dengan indikator jumlah osteoklas, osteoblas dan kolagen. Metode: Penelitian ini menggunakan 28 marmot yang dibagi menjadi 4 kelompok penelitian. Pencabutan dilakukan pada incisive rahang bawah kemudian soket pencabutan diisi dengan CMCNa 3% pada kelompok control; spirulina 3% chitosan 200 mg pada kelompok perlakuan 1; spirulina 6 % chitosan 200 mg pada kelompok perlakuan 2, dan spirulina 12% citosan 200 mg pada kelompok perlakuan 3. Pada hari ke 30 dilakukan pemeriksaan histopatologi menggunakan mikroskop untuk menghitung jumlah osteoblas, osteoklas dan kolagen. Hasil: Data dianalisis dengan Anova dan Tukey HSD. Jumlah osteoklas tidak berbeda secara signifikan antara setiap kelompok, sedangkan jumlah osteoblas dan kolagen terdapat perbedaan yang signifikan antara kelompok. Hasil penelitian menunjukkan bahwa induksi kombinasi gel spirulina chitosan mampu mengakumulasi serat kolagen dan menghasilkan penyembuhan luka lebih cepat. Simpulan: Kombinasi gel spirulina 12% chitosan 200 mg dapat digunakan sebagai bahan alternatif untuk remodeling tulang yang lebih baik setelah pencabutan gigi.
Pengaruh posisi dan fraksi volumetrik fiber polyethylene terhadap kekuatan fleksural fiber reinforced composite (The effect of position and volumetric fraction polyethylene fiber on the flexural strength of fiber reinforced composite) Septommy, Catur; Widjijono, Widjijono; Dharmastiti, Rini
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p52-56

Abstract

Background: Composite resin is a combination of filler and matrix. The additional of fiber in the composite resin has a function as load-bearing in mastication. Polyethylene fiber has been used as a reinforced to receive the forces on the fixed denture fiber reinforced composite (FRC). Purpose: The purpose of this study was to determine effect of position and olumetric fraction of fibers on the flexural strength and modulus polyethylene FRC. Methods: This study used 7 groups with variations in the position and the volume of fiber. Group I, position compression volume 1 sheet; group II, 2 volume compression sheet position; group III, volume 1 sheet neutral position; group IV, neutral position volume 2 sheets; group V, position tension volume 1 sheet; group VI position tension volume 2 sheets; and group VII without fiber. Each group consisted of 6 samples and FRC rod-shaped samples with size (25 x2 x 2) mm. Samples were tested by three-point bending test with a universal testing machine. The data were analyzed by two-way Anova and LSD test continued (α = 0,05). Results: Group VI had the highest mean flexural strength than others (360.74 MPa) and group IV had the highest flexural modulus than others (3.56 GPa). The flexural strength and modulus with the variation of position or volume showed a significant differences (p<0.05), while the interaction between position and volume showed no significant difference (p>0.05). Conclusions: The position fiber on tension and additional two strips on volumetric fiber affected the increasing flexural strength and modulus of FRC.Latar belakang: Komposit merupakan gabungan filler dan matriks. Penambahan fiber pada komposit berfungsi sebagai penahan beban pengunyahan. Polyethylene fiber telah digunakan sebagai penguat dalam menerima gaya-gaya pada gigi tiruan cekat fiber reinforced composite (FRC). Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh posisi dan fraksi volumetrik fiber pada kekuatan fleksural polyethylene FRC. Metode: Penelitian ini membuat 7 kelompok sampel polyethylene FRC dengan variasi posisi dan volume fiber. Kelompok I, posisi compression volume 1 lembar; kelompok II, posisi compression volume 2 lembar; kelompok III, posisi netral volume 1 lembar; kelompok IV, posisi netral volume 2 lembar; kelompok V, posisi tension volume 1 lembar; kelompok VI, posisi tension volume 2 lembar; dan kelompok VII, tanpa fiber. Setiap kelompok terdiri atas 6 sampel dan sampel berbentuk batang FRC dengan ukuran (25x2x2) mm. Sampel diuji dengan three-point bending test dengan universal testing machine. Data dianalisis dengan two-way anova dan dilanjutkan uji LSD (α=0.05). Hasil: Rerata kekuatan fleksural kelompok VI paling tinggi (360.74 MPa) dan kelompok IV memiliki modulus fleksural tertinggi (3.56 GPa). Kekuatan dan modulus fleksural dengan variasi posisi atau volume menunjukkan perbedaan bermakna (p<0.05) sedangkan interaksi antara posisi dan volume menunjukkan perbedaan tidak bermakna (p>0.05). Simpulan: Posisi fiber pada sisi tension FRC dan penambahan volume 2 lembar fiber akan meningkatkan kekuatan fleksural FRC.
Alkaline phosphatase expression during relapse after orthodontic tooth movement Pudyani, Pinandi Sri; Asmara, Widya; Ana, Ika Dewi; Utari, Tita Ratya
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p25-30

Abstract

Background: The increasing of osteoblast activities during bone formation will be accompanied with the increasing expression of alkaline phosphatase enzyme (ALP). ALP can be obtained from clear fluid excreted by gingival crevicular fluid (GCF). Bone turnover, especially bone formation process, can be monitored through the expression of ALP secreted by GCF during orthodontic treatment. Thus, retention period is an important period that can be monitored through the level of bone metabolism around teeth. Purpose: This research were aimed to determine the relation of distance change caused by tooth relapse and ALP activities in gingival crevicular fluid after orthodontic; and to determine ALP as a potential biomarker of bone formation during retention period. Methods: Lower incisors of 25 guinea pigs were moved 3 mm to the distally by using open coil spring. Those relapse distance were measured and the gingival crevicular fluid was taken by using paper points to evaluate ALP levels on days 0, 3, 7, 14 and 21 respectivelly by using a spectrophotometer (405 nm). t-test and ANOVA test were conducted to determine the difference of ALP activities among the time intervals. The correlation regression analysis was conducted to determine the relation of distance change caused by the relapse tooth movement and ALP activities. Results: The greatest relapse movement was occurred on day 3 after open coil spring was removed. There was significant difference of the average of distance decrease among groups A1-A5 (p<0.05). It was also known that ALP level was increased on day 3, but there was no significant difference of the average level of ALP among groups A1-A5 (p>0.05). Finally, based on the results of correlation analysis between the ALP level decreasing and the relapse distance on both right and left of mesial and distal sides, it is known that there was no relation between those two variables (p>0.05). Conclusion: It can be concluded that relapse after orthodontic tooth movement occurs rapidly as the teeth are free from orthodontic force. ALP level can be detected through gingival crevicular fluid during relapse by using a spectrophotometer.Latar belakang: Peningkatan aktivitas osteoblas selama pembentukan tulang akan disertai peningkatan ekspresi enzim alkalin fosfatase (Alkaline Phosphatase/ALP). Sumber ALP dapat diperoleh dari cairan bening yang diekskresi celah gingiva gigi yang dikenal sebagai cairan krevikuler gingiva (gingival crevicular fluid/GCF). Bone turnover terutama proses pembentukan tulang dapat dimonitor melalui ekspresi ALP cairan krevikuler gingiva selama perawatan ortodonti. Periode retensi merupakan periode yang penting. Kesulitan memecahkan masalah retensi akan dapat ditangani dengan memonitor tingkat metabolisme tulang disekitar gigi. Tujuan: Penelitian ini bertujuan untuk meneliti perubahan jarak relapse gigi dan aktivitas ALP pada cairan krevikuler gingiva setelah digerakkan secara ortodonti dan potensi ALP sebagai biomarker pembentukan tulang pada periode retensi. Metode: Gigi insisivus bawah 25 ekor marmot digerakkan ke distal menggunakan opencoil spring sampai mencapai jarak ± 3 mm, diukur gerakan relapse gigi dan pengambilan cairan krevikuler gingiva menggunakan paper point untuk dievaluasi kadar ALP pada hari ke 0, 3, 7, 14 dan 21 menggunakan spektrofotometer (405 nm). Analisis t-test dan anova untuk mengetahui perbedaan aktivitas ALP antar interval waktu dan analisis regresi korelasi untuk mengetahui hubungan besarnya jarak relapse dengan aktivitas ALP. Hasil: Pergerakan relapse yang paling besar terjadi pada hari ke 3 setelah opencoil spring dilepas. Terdapat perbedaan rata-rata penurunan jarak antar kelompok A1-A5 yang signifikan (p<0,05). Kadar ALP mengalami peningkatan pada hari ke 3, namun tidak terdapat perbedaan rata-rata yang signifikan kadar ALP antar kelompok A1-A5 (p>0,05). Hasil uji korelasi antara penurunan jarak dengan kadar ALP pada mesial distal gigi baik kanan maupun kiri tidak menunjukkan adanya hubungan kedua variabel (p>0,05). Simpulan: Relapse pada perawatan ortodonti terjadi secara cepat ketika gigi terbebas dari gaya ortodonti. Kadar ALP dapat terdeteksi dari cairan krevikuler gingiva pada pergerakan relapse gigi menggunakan spektrofotometer.
Oral hygiene and number of oral mucosal lesion correlate with oral health-related quality of life in elderly communities Agustina, Dewi
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p57-61

Abstract

Background: Quality of life assessment mostly is based on general health. Deterioration of physiologic condition, polypharmacy and the high occurrence of chronic disease in elderly may manifest in oral cavity that can affect oral function, in turn it will affect quality of life of elderly. Purpose: This study was aimed to determine the correlation of oral health status and oral health-related quality of life (OHRQoL) in elderly communities of Yogyakarta city. Method: Seventy three elders were subjects of this study. Data of OHRQoL and oral health status were obtained from modification of questionnaire of Dental Impact of Daily Living (DIDL) Index and from intraoral examination, respectively. Intraoral examination comprised oral mucosal lesion amount, oral hygiene, DMFT index and periodontal tissue status. The data then were analyzed statistically using Pearson Product Moment Correlation. Result: The results showed that mean of DMFT index was 16.9 and 63% of subjects were found with gingivitis, most subject had moderate oral hygiene and each subject at least had two oral mucosal lesions. Mean score of quality of life was 27.2 and classified as satisfying. Oral hygiene and number of oral mucosal lesion had correlation with OHRQoL with r were -0.236 (Sig. : 0.045) and -0.288 (Sig. : 0.013), respectively. Conclusion: The study suggested that oral hygiene and number of oral mucosal lesion correlate with oral health related-quality of life in elderly communities of Yogyakarta city.Latar belakang: Penilaian kualitas hidup terutama didasarkan pada kesehatan umum. Memburuknya kondisi fisiologis, polifarmasi dan tingginya kejadian penyakit kronis pada lansia dapat termanifestasi di dalam rongga mulut sehingga dapat mempengaruhi fungsi mulut yang pada gilirannya akan mempengaruhi kualitas hidup lansia. Tujuan: Penelitian ini bertujuan untuk meneliti hubungan antara status kesehatan mulut dan kualitas hidup berdasarkan kesehatan mulut pada masyarakat lanjut usia di kota Yogyakarta. Metode: Tujuhpuluh tiga lansia sebagai subjek dalam penelitian ini. Data kualitas hidup berdasarkan kesehatan mulut dan status kesehatan mulut diperoleh dari modifikasi kuesioner Indeks Dampak Kesehatan Gigi terhadap Kehidupan Sehari-hari dan dari pemeriksaan intraoral. Pemeriksaan intra oral terdiri atas jumlah lesi mukosa rongga mulut, kebersihan mulut, indeks DMFT dan status jaringan periodontal. Data kemudian dianalisis secara statistik menggunakan Pearson product moment correlation. Hasil: Hasil penelitian menunjukkan bahwa rata-rata indeks DMFT adalah 16,9 dan 63% subjek ditemukan dengan gingivitis, subjek rata-rata memiliki kebersihan mulut yang cukup dan setiap subjek rata-rata memiliki dua lesi mukosa mulut. Rerata kualitas hidup berdasarkan kesehatan mulut adalah 27,2 dan tergolong memuaskan. Kebersihan mulut dan jumlah lesi mukosa mulut memiliki korelasi dengan kualitas hidup berbasis kesehatan rongga mulut dengan masing-masing r adalah -0,236 (Sig. : 0,045) dan -0,288 (Sig. : 0,013). Simpulan: Dapat disimpulkan bahwa kebersihan mulut dan jumlah lesi mukosa mulut berkorelasi dengan kualitas hidup berbasis kesehatan rongga mulut pada masyarakat lanjut usia di kota Yogyakarta.
Antimicrobial proteins of Snail mucus (Achatina fulica) against Streptococcus mutans and Aggregatibacter actinomycetemcomitans DN, Herluinus Mafranenda; Kriswandini, Indah Listiana; R, Ester Arijani
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p31-36

Abstract

Background: Achasin and mytimacin-AF are proteins of snail mucus (Achatina fulica) which have antimicrobial activity. Snail mucus is suspected to have other proteins which have antimicrobial activity against Streptococcus mutans and Aggregatibacter actinomycetemcomitans the oral pathologic bacteria. Purpose: The study were aimed to characterize the proteins of snail mucus (Achatina fulica) that have antimicrobial activities to Streptococcus mutans and Actinobacillus actinomycetemcomitans, and to compared the antimicrobial effect of achasin and mytimacin-AF. Methods: The sample of study was the mucus of snails which were taken from Yogyakarta Province. The isolation and characterization of protein were conducted by using SDS-PAGE method, electroelution, and dialysis. Nano drop test was conducted to determine protein concentration. The sensitivity test was conducted by using dilution test, and followed by spectrophotometry and paper disc diffusion tests. Results: The study showed that proteins successfully characterized from snail mucus (Achatina fulica) were proteins with molecular weights of 83.67 kDa (achasin), 50.81 kDa, 15 kDa, 11.45 kDa (full amino acid sequence of mytimacin-AF) and 9.7 kDa (mytimacin-AF). Based on the dilution test, Achasin had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans. But the paper disc diffusion test result showed that Achasin had antimicrobial activities against Streptococcus mutans and Aggregatibacter actinomycetemcomitans, while mytimacin-AF had no antimicrobial activities. Conclusion: The proteins with molecular weights of 50.81 kDa, 15 kDa, 11.45 kDa were considered as new antimicrobial proteins isolated from snail mucus. Achasin, had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans.Latar belakang: Achasin dan mytimacin-AF adalah protein lendir bekicot (Achatina fulica) yang memiliki aktivitas antimikroba. Lendir bekicot diduga memiliki protein lain yang memiliki aktivitas antimikroba terhadap Streptococcus mutans dan Actinobacillus actinomycetemcomitans bakteri patologis oral. Tujuan: Penelitian ini bertujuan untuk mengkarakterisasi protein lendir bekicot (Achatina fulica) yang memiliki aktivitas antimikroba terhadap Streptococcus mutans dan Aggretibacter actinomycetemcomitans, dan membandingkan efek antimikroba protein achasin dan mytimacin-AF. Metode: Sampel penelitian adalah lendir bekicot yang diambil dari Provinsi Yogyakarta. Isolasi dan karakterisasi protein dilakukan dengan metode SDS-PAGE, elektro-elusi, dan dialisis. Nano drop test dilakukan untuk menentukan konsentrasi protein. Uji sensitivitas dilakukan dengan menggunakan uji dilusi, dan diikuti oleh spektrofotometri dan tes difusi kertas cakram. Hasil: Protein dari lendir bekicot (Achatina fulica) yang ditemukan adalah protein dengan berat molekul 83,67 kDa (achasin), 50,81 kDa, 15 kDa, 11,45 kDa (urutan asam amino penuh mytimacin-AF) dan 9,7 kDa (mytimacin- AF). Berdasarkan uji dilusi, Achasin memiliki aktivitas antimikroba yang lebih baik terhadap Streptococcus mutans, sedangkan mytimacin-AF memiliki aktivitas antimikroba yang lebih baik terhadap Aggregatibacter actinomycetemcomitans. Namun hasil uji difusi cakram kertas menunjukkan bahwa Achasin memiliki aktivitas antimikroba terhadap Streptococcus mutans dan Aggegatibacter actinomycetemcomitans, sementara mytimacin-AF tidak memiliki kegiatan antimikroba. Simpulan: Protein dengan berat molekul 50,81 kDa, 15 kDa, 11,45 kDa merupakan protein antimikroba baru diisolasi dari lendir bekicot. Achasin, memiliki aktivitas antimikroba yang lebih baik terhadap Streptococcus mutans, sedangkan mytimacin-AF memiliki aktivitas antimikroba yang lebih baik terhadap Actinobacillus actinomycetemcomitans.
Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya Kresnoadi, Utari; Rahayu, Retno Pudji
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p1-6

Abstract

Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb). As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan pembuatan gigitiruan. Diperlukan preservasi soket pencabutan gigi asli pada penderita untuk mencegah terjadinya resorpsi tulang alveolar. Aloe vera mempunyai komponen anthraquinon yaitu aloin, Aloe emodin, barbaloin yang merupakan anti inflamasi yang dapat secara cepat menyembuhkan luka, sehingga berpotensi untuk digunakan pada preservasi soket. Didalam mengatasi keradangan peran NF-kb sangat berarti, sebab penurunan NF-kb akan mengurangi terjadinya inflamasi. tujuan: Penelitian ini bertujuan untuk menguji apakah induksi kombinasi lidah buaya dan XCB ke soket pencabutan gigi dapat mengurangi peradangan dengan mengurangi ekspresi NF - kb , osteoklas dan osteoblast. Metode: Empat puluh delapan ekor Cavia cabaya yang terdiri dari 8 kelompok, tiap kelompok 6 ekor, kelompok pengisian PEG (kontrol), kelompok pengisian XCB, kelompok pengisian aloe vera dan kelompok pengisian kombinasi aloe vera dan XCB, kelompok ini terdiri dari kelompok 7 dan 30 hari, kemudian diperiksa dengan imunohistokimia ekspresi NF-kb dan pemeriksaan histologi untuk osteoblas dan osteoklas. Hasil: Kelompok yang diisi kombinasi Alo vera dan xenograft concelous bovine pada soket pencabutan gigi, menunjukan nilai tertinggi dalam pertumbuhan osteoblas dan penurunan pada ekspresi NF-kb dan osteoklas. Simpulan: Induksi kombinasi Aloe vera dan xenograft concelous bovine pada preservasi soket pencabutan gigi dapat menurunkan ekspresi NF-kB dan osteoklas, menurunkan resiko resorpsi tulang alveolar dan meningkatkan osteoblas.
The effect of soda immersion on nano hybrid composite resin discoloration Effendi, M. Chair; Nugraeni, Yuli; Pratiwi, Rizki Widya
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p37-40

Abstract

Background: Composite resin is the tooth-colored restorative material which most of the people are fond of due to their aesthetic value. The composite resin discoloration may happen because of the intrinsic and extrinsic factors. Soda water is one of the beverages which can cause the composite resin discoloration. Purpose: The study was aimed to determine the effect of soda immersion on nano hybrid composite resin discoloration. Methods: The study was an experimental laboratory study using 100 shade A3 nano hybrid composite resin specimens with the diameter of 5 mm and density of 2mm. The samples were divided into 5 groups, each group was immersed in different beverages. The beverages were mineral water; lemon-flavored soda; strawberry-flavored soda; fruit punch-flavored soda; and orange-flavored soda for 3, 7, 14 and 21 days respectively, in the temperature of 37o C. The discoloration measurement utilizes Spectrophotometer, Vita Easy Shade, and uses CIEL*a*b* method. Results: The result showed that the duration of immersion in soda had an effect on the Nano hybrid composite resin discoloration. Strawberry and fruit punch- flavored soda were the most influential components toward the discoloration. Nevertheless, the generally-occurred discoloration was clinically acceptable (∆E ≤ 3,3). Conclusion: The study suggested that the soda immersion duration has effect on Nano hybrid composite resin discoloration.Latar belakang: Resin komposit adalah material sewarna gigi yang diminati masyarakat karena memiliki nilai estetik yang baik. Perubahan warna resin komposit dapat terjadi karena faktor intrinsik dan ekstrinsik. Minuman soda merupakan salah satu minuman yang dapat menyebabkan perubahan warna pada resin komposit. Tujuan: Tujuan dari penelitian ini untuk meneliti perubahan warna resin komposit nanohibrida akibat perendaman dalam minuman soda. Metode: Metode yang digunakan pada penelitian ini adalah eksperimental laboratorik dengan menggunakan 100 spesimen resin komposit nanohibrida shade A3 berdiameter 5 mm dan tebal 2 mm. Sampel dibagi dalam 5 kelompok, masing-masing kelompok direndam dalam minuman yang berbeda, yaitu direndam dalam air mineral, soda lemon, soda strawberi, soda fruitpunch, dan soda jeruk selama 3, 7, 14 dan 21 hari, dalam suhu 37o C. Pengukuran perubahan warna menggunakan Spectrophotometer, Vita Easy Shade dengan metode CIEL*a*b*. Hasil: Hasil penelitian menunjukkan bahwa lama perendaman pada minuman soda berpengaruh terhadap perubahan warna resin komposit nanohibrida. Soda yang paling berpengaruh terhadap perubahan warna adalah soda strawberi dan soda fruitpunch. Namun perubahan warna yang terjadi secara umum masih dapat diterima secara klinis (∆E ≤ 3,3). Simpulan: Hasil penelitian ini menunjukkan bahwa lamanya waktu perendaman mempengaruhi perubahan warna pada resin komposit nanohibrida.
The role of Hsp0, CD-8 and IFN-γ in immunopathobiogenesis of periapical granuloma in dental caries Cilmiaty, Risya; Rukmo, Mandojo
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p7-12

Abstract

Background: The incidence of dental caries with periapical granulomas in Indonesia is quite high. However, the mechanism of the formation of periapical granulomas in dental caries caused by bacterial infection in immunopathobiogenesis cannot be explained completely. Thus, this explanation is necessary in order to be used as a basis for diagnostic, preventive and therapeutic measures. Purpose: This research was aimed to determine the role of Hsp60, CD-8 and IFN-γ in immunopatobiogenesis of periapical granuloma in dental caries. Methods: This research was an analytic observational study with cross sectional approach. Samples of this research were 36 teeth of patients with dental caries, consisting of 18 caries teeth with periapical granulomas and 18 caries teeth without periapical granulomas. The variables observed in this research were Hsp60, CD-8 and IFN-γ. Measurements were conducted by using immunohistochemical methods on periapical tissue. Results: The mean of Hsp60, CD-8 and IFN-γ in granuloma group was significantly higher than those in non granuloma group (p<0.05). The positive role of IFN-γ on the incidence of granulomas appeared to be more prominent. Conclusion: The study suggested that in immunopathobiogenesis of periapical granuloma in dental caries, Hsp60, CD-8 and IFN-γ played important roles, but the role of IFN-γ was found to be more prominent.Latar belakang: Angka kejadian gigi karies dengan granuloma periapikal di Indonesia cukup tinggi, Namun mekanisme terbentuknya granuloma periapikal pada gigi karies yang disebabkan oleh infeksi bakteri secara imunopatobiogenesis belum dapat dijelaskan secara tuntas. Adanya penjelasan ini diperlukan agar dapat digunakan sebagai dasar pengembangan diagnosis, langkah preventif dan terapinya. Tujuan: Penelitian ini bertujuan untuk mengetahui peran Hsp60, CD-8 dan IFN-γ dalam immunopatobiogenesis dari granuloma periapikal karies gigi. Metode: Penelitian ini merupakan penelitian observasional analitik dengan pendekatan cross sectional. Sampel dari penelitian ini adalah 36 gigi pasien dengan karies, yang terdiri dari 18 karies gigi dengan granuloma periapikal dan 18 karies gigi tanpa granuloma periapikal. Variabel yang diamati adalah Hsp60, CD-8 dan IFN-γ. Pengukuran dilakukan dengan menggunakan metode imunohistokimia pada jaringan periapikal. Hasil: Rerata Hsp60, CD-8 dan IFN-γ pada kelompok granuloma secara signifikan lebih tinggi dibanding kelompok non granuloma (p <0,05). Peran positif dari IFN-γ terhadap kejadian granuloma tampaknya lebih menonjol. Simpulan: Studi ini menunjukkan bahwa dalam imunopatobiogenesis dari granuloma periapikal karies gigi, Hsp60, CD-8 dan IFN-γ memainkan peran penting, tetapi peran IFN-γ ditemukan lebih menonjol.
The role of heat shock protein (HSP ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture Mulyani, Sri Wigati Mardi; Setiawati, Ernie Maduratna; Safitri, Erma; Astuti, Eha Renwi
Dental Journal (Majalah Kedokteran Gigi) Vol 47, No 1 (2014): (March 2014)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v47.i1.p41-44

Abstract

Background: The concept of stem cell therapy is one of the new hope as a medical therapy on salivary gland defect. However, the lack of viability of the transplanted stem cells survival rate led to the decrease of effectiveness of stem cell therapy. The underlying assumption in the decrease of viability and function of stem cells is an increase of apoptosis incidence. It suggests that the microenvironment in the area of damaged tissues is not conducive to support stem cell viability. One of the microenvironment is the hypoxia condition. Several scientific journals revealed that the administration of hypoxic cell culture can result in stress cells but on the other hand the stress condition of the cells also stimulates heat shock protein 27 (HSP 27) as antiapoptosis through inhibition of caspase 9. Purpose: The purpose of this study was to examine the role of heat shock protein 27 as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture. Methods: Stem cell culture was performed in hypoxic conditions (O2 1%) and measured the resistance to apoptosis through HSP 27 and caspase 9 expression of bone marrow mesenchymal stem cells by using immunoflorecence and real time PCR. Results: The result of study showed that preconditioning hypoxia could inhibit apoptosis through increasing HSP 27 and decreasing level of caspase 9. Conclusion: The study suggested that hypoxic precondition could reduce apoptosis by increasing amount of heat shock protein 27 and decreasing caspase 9.Latar belakang: Konsep terapi stem cell merupakan salah satu harapan baru sebagai terapi medis kelainan kelenjar ludah. Namun, rendahnya viabilitas stem cell yang ditransplantasikan menyebabkan penurunan efektivitas terapi. Asumsi yang mendasari rendahnya viabilitas dan fungsi stem cell adalah tingginya kejadian apoptosis. Hal ini menunjukkan bahwa lingkungan mikro di daerah jaringan yang rusak tidak kondusif untuk mendukung viabilitas stem cell. Salah satu lingkungan mikro adalah kondisi hipoksia. Beberapa jurnal ilmiah mengungkapkan bahwa kondisi hipoksia pada kultur sel dapat menyebabkan sel-sel stres, namun di sisi lain kondisi stres sel juga merangsang heat shock protein 27 (HSP 27) sebagai antiapoptosis dengan menghambat ekspresi caspase 9. Tujuan: Tujuan penelitian ini adalah untuk meneliti peran protein heat shock 27 sebagai inhibitor apoptosis dalam kondisi hipoksia kultur stem cell sumsum tulang. Metode: Kultur stem sel dilakukan dalam kondisi hipoksia (O2 1%) dan mengukur resistensi terhadap apoptosis melalui ekspresi HSP 27 dan caspase 9 stem cell mesenchymal sumsum tulang dengan menggunakan immunoflorecence dan PCR real time. Hasil: Hasil penelitian menunjukkan bahwa prakondisi hipoksia dapat menghambat apoptosis melalui peningkatan HSP 27 dan penurunan tingkat Caspase 9. Simpulan: Studi ini menunjukkan bahwa prakondisi hipoksia dapat mengurangi apoptosis dengan meningkatkan jumlah protein heat shock 27 dan penurunan caspase 9.

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