Welcome to Journal of Islamic Pharmacy (e-ISSN : 2527-6123) formerly Jurnal Farmasains (p-ISSN : 2460-5182) Department of Pharmacy, Universitas Islam Negeri Maulana Malik Ibrahim Malang, Indonesia. The journal was established in 2015 and online publication was begun in 2016. Since 2016, the journal has been published in English and only receives manuscripts in English.
Monosodium Glutamat activates an axis ablation in arcuate nucleus hypothalamus which happens to possibly initiate malfunctions in Hipothalamic Pituitary Adrenal axis. Yet, it is known that hypothalamus produces Gonadotropin Releasing Hormone which is able to stimulate the secretion of Gonadotropin Follicle Stimulating Hormon and Luteinizing Hormone from the anterior pituitary gland. These two hormones, however, are essentials for the development of gonad and during the cycles of oogenesis. Hence, any malfunctions resided in the humanâs hypothalamus can possibly put endocrine gland into troubles, such as reproduction hormones which affect the workings of gonad. Therefore, the extract of Temulawak is promoted to be the attempt to resolve the issue for its antioxidant properties. Temulawak extract contains antioxidant, properties for as much as 87.01 ppm, according to Rosidi researchâs result, analyzing the Temulawakâs potentials. The amount of antioxidant found Temulawak extract is categorized as active and applicable as natural antioxidant source. However, this research adopts true experimental post-test only control group design. It includes 5 (five) groups of female mice as which are K1 (CMC), K2 (CMC+MSG), P1 (MSG+0.4 mg Temulawak extract), P2 (MSG+0.8 MG Temulawak extract), P3 (MSG+1.2 mg Temulawak extract). In addition, the miceâs ovaries are removed to be subsequently tested using Hematoxillin-Eosin and the ovariesâ follicles quantity is observed and counted. The data is analyzed using One Way ANOVA. The research finally comes with a result showing that there are not any significant differences discovered from the ovariesâ follicles quantity from the five tested groups (p>0.05). Therefore, it is implied that Temulawak extract does not provide any influential results to the house miceâs ovariesâ follicles quantity which has been exposed to MSG.Â Keywords: Monosodium Glutamate (MSG), Temulawak (Curcuma xanthorriza Roxb.), Ovarian follicles quantityÂ
Calotropis gigantea radix is one of plant which has bioactive component as antimalarial.The purpose of this research are to know antimalarial activity from combination of Calotropis gigantea radix extract and artemisin. The research consist of extraction of Calotropis gigantea radix was done with extraction maseration method. Extraction was performed by maseration withÂ 80% ethanol solvent. Concentrated extract was in vivo antimalarial tested to animal model. Mice were peritoneal infected with 106Plasmodium berghei ANKA and divided into 5 treatment groups: negative control; positive control (artemisin of dose 4 mg/kgBW); combination of artemisin(1/4 dose of artemisin standar) and Calotropis giganteain 3 doses: 0,1 mg/KgBW; 1 mg/KgBWand 10 mg/KgBW. Data of inhibition was analyzed using SPSS program with Two Way ANOVA Test dan continued with Tukey Test.The result shows that combination of Calotropis gigantea radix extract and artemisin with dose 10 mg/KgBB has higher antimalarial activity than control positive (artemisin). The value of parasite inhibition is 55,2% for dose 0.1 mg/KgBW ; 72,8% for dose 1 mg/KgBW ; 87,3% for dose 10 mg/KgBW and 56,06 mg/KgBW for control positive (artemisin). The phytochemical compounds in 80%Â ethanol solvent extract are tannins and steroids.Â Keywords: Calotropis gigantea,antimalaria, combination, artemisin, Plasmodium berghei
The prevalence of toxoplasmosis in Indonesia at 2006 was 43-88%. Drugs that have been the most widely used for the treatment of patients with toxoplasmosis include pyrimethamine and spiramicin. However, these drugs have been reported to cause side effects such as bone marrow suppression, penetrate the blood brain barrier, crossed the placenta and fetal blood. Therefore necessary to find alternative drugs for toxoplasmosis effective and safe. This research studied the effect of alkaloid fraction of leaves of plants Alstonia scholaris to the number takizoit profiles in intraperitoneal fluid of mice infected with Toxoplasma gondii. This research used the experimental animals were grouped as the control group and the treatment group. In the treatment group, each group was given treatment with fraction extract alkaloids from leaves of plants Pulai. The results showed the number takizoit mice infected with Toxoplasma gondii parasites without therapy is 957.5 x 103 whereas the alkaloid fraction of the ethanol extract of leaves is 737.5 x 103.Â Keywords: Leaves of Pulai (Alstonia scholaris), Toxoplasma gondii, toxoplasmosis.
Thistle (Calotropisgigantea) is a wild plant that contains secondary metabolites as a potential antimalarial drugs.This study aims to determine the content of active compound contained, the potential of ethanol extract of the stem thistle as an antimalarial, and knowing the best eluent used when TLC test.This study covers the extraction of stem thistle using maceration for 24 hours using ethanol 80%.Stirring assisted with shaker for 3 hours. Ekstrak concentrated phytochemical test, antimalarial activity was evaluated by experimental animals mice infected by Plasmodium berghei.Data degree of parasitaemia in mice were analyzed using Minitab 16 with Test TwoWay ANOVA followed by Tukey test.The results showed that ethanol extract stem thistle contains the active compound class of triterpenoids, and potentially as an antimalarial with ED50 values 152.878 mg / kg bw were included in both categories.TLC test results showed that the best eluent for the separation of triterpenoids of stem thistle extract is benzene: chloroform (3: 7) which produced 12 spots with Rf values of 0.04;0.06;0.14;0.21;0.29;0.35;0.41;0.48;0.80;0.84;0.88;0.91.Â Keywords: Antimalarial, Calotropisgigantea, Ethanol 80%, Plasmodium berghei, Thin Layer Chromatography
The purposes of this research are to know the effect of C.pubescens fruit extract on the amount of gingival fibroblasts in wound healing of Rattus norvegicus mouth cavityâs mucosal and to know the effect of C.pubescens comes from two different areas (Dieng and Cangar) on the amount of gingival fibroblasts in wound healing of Rattus norvegicus oral mucosa. Twenty eight rats are divided to be four groups (K1, K2, K3, and K4), each rat is wounded 1 cm on the gingival mucosa of lower jaw (specifically on the apical region of incisive teeth). K1 is the control group with aquadest treatment on the wound. The wound in the K2 is treated with C.pubescens fruit extract from Dieng. The wound in the K3 is treated with C.pubescens fruit extract from Cangar. And the treatment for K4 is medicated by policresulen (common medicine for oral mucosal wound). On the fifth day of the treatment, all rats are sacrificed, and the gingival tissue is taken up for the next step. Gingival tissue is smeared by Haematoxylin Eosin (HE) to analyze the amount of gingival fibroblasts histologically. The result of this research shows that the highest average amount of gingival fibroblasts comes from K4 (policresulen treatment). And there is no significant difference on the number of Rattus norvegicus gingival fibroblasts from all of the groups (K1, K2, K3, and K4).Â Keywords: fibroblast, gingival, wound, mucosa, mouth, C.pubescens
Catechins Green tea clones GMB4 are potential natural subtances to inhibitÂ inflammation process in atherosclerosis. The purpose of this research to determine activity ofÂ Catechins Green tea clones GMB4 to decrease TNF-Î± levels, as well as to determine optimal dose of Catechins. This research was conducted at the Laboratory of Physiology of the University of Brawijaya. The method used in this research is completely randomized design with five treatments: (1) Mice with standard feed (2) mice with high-fat diet (3) mice with high-fat diet + catechins 3mg / day (4) mice with a diet high fat + catechin 6mg / day (5) mice with high-fat diet + catechin 24mg / day. The treatment was done for 60 days and measured levels of TNF-Î± at the end of the research. The results showed that Catechins green tea clones GMB4 can inhibit the inflammatory process in atherosclerosis through decreasing levels of TNF-Î± at doses of 3mg / day. The conclusion of this research is catechins at a dose of 3 mg / day can inhibit inflammation in atherosclerosis significantly.Â Keywords : GMB 4 green tea clones, Catechins, inflammation, TNF-Î±
Diarrhoeal diseases are still a major cause of the high morbidity and mortality of children globally, especially in children under five years. Diarrhea caused by Shigella flexneri usual dealt with antibiotics seen it is getting resistant with various antibiotic. Â Prevention shigellosis with a vaccine that was developed and used now are still limited. This study attempts to explore hemaglutinin protein pili S . flexneri allegedly role in adhesion bacteria on the mice enterocyte. This research using Â hemaglutination test then adhesion test Â by means of reacting protein sub unit pili 18 kDa S. flexneri with mice enterocyte cells and observed the amount of bacteria inherent in a mice cell enterocyte. The research found that proteins sub unit pili with molecules weight 18 kDa is a protein that capable to agglutinate mice red blood cells with highest dilution and capable to inhibit adhesion bacteria on the mice enterocyte significantly.Â Keywords: Shigella flexneri, protein sub unit pili , hemagglutinin protein, adhesin protein.
The aim of this research is to determine the influence of jamu Madura âEmpot Superâ (JMES) on the vaginal epithelium thickness of Rattus norvegicus in vivo. This research is kind of âtrue experimental-post test only control group designâ. The rats were given drinking JMES once daily PS (Per-Sonde) for a month, then the vagina was taken to be sample for HE colouring. The sample was observed by the binocular microscope (100 times magnification) to identify the changes in the thickness of their vaginal epithelium. Calculation of the vaginal epithelium thickness was counted on the 10 field of view chosen randomly by the blind method. The result show that the vaginal epithelium thickness increased with dose 0,17mg/BW, 0,34mg/BW, and 0,68mg/BW of JMES compared with negative control group. But, the vaginal epithelium thickness decrease at the dose 0,51mg/BW compared with negative control group.Â Keywords: Jamu Madura âEmpot Superâ (JMES), vaginal epithelium thickness, white mice (Rattus norvegicus), In Vivo study
Permen yang dijual di pasaran diduga mengandung sejumlah logam berat misalnya logam tembaga. Dalam penelitian ini, sampel permen didestruksi dengan HNO3, HNO3/H2SO4Â (3:1) dan HNO3/H2SO4/H2O2Â (6:2:1) untuk mengetahui zat pengoksidasi terbaik logam tembaga pada permen. Selanjutnya dilakukan pengukuran kadar logam tembaga dengan Spektrofotometer Serapan Atom (SSA). Hasil analisis uji one way anove dengan uji F menunjukkan bahwa zat pengoksidasi terbaik logam tembaga dalam sampel permen adalah HNO3/H2SO4. Sementara itu, perolehan kadar logam tembaga pada permen dengan label A, B, C, D, dan E berturut-turut sebesar 1,72 mg/Kg; 1,69 mg/Kg; 1,57 mg/Kg; 1,71mg/Kg; dan 1,69 mg/Kg. Dengan demikian, kadar logam tembaga pada permen yang dijual di pasaran masih berada dalam batas aman konsumsi yang ditetapkan oleh SNI.Â Kata Kunci:Â permen, destruksi basah, oksidator, waktu stabilitas, Spektrofotometri Serapan Atom (SSA)
Osteoarthritis is a chronic disorder of synovial joints, characterized by progressive softening and disintegration of cartilage in joints. This is the most common type of arthritis in Indonesia with prevalence about 23.6 to 31.3% and generally suffered by middle age patients. Drug therapy for treating osteoarthritis is NSAIDs, supplements and corticosteroids. The increasing number of available drugs, drug users and more complex drug regimens causedÂ more side effect and potential drug interaction andÂ lead to another problem, it is Drug Related Problems. The purpose of this study was to identify potential Drug Related Problem categories of improper dosage and Adverse Drug Reactions in osteoarthritis outpatient in RSUD Jombang during 2016. This research is a non-experimental descriptive study conducted retrospectively, carried out in March 2017 at RSUD Jombang.Â Research sample is 87 respondents of osteoarthritis outpatient which taken by systematic random sampling method. The data presented in percentage of improper dosage and Adverse Drug reactions potential case. The result found potential of improper dosage in respondent about 82,76% and Adverse Drug Reactions about 20,69%.