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Jurnal Ilmu dan Kesehatan Hewan
Published by Universitas Udayana
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Core Subject : Health,
Jurnal Ilmu dan Kesehatan Hewan menerima naskah ilmiah original (belum pernah dipublikasikan) dalam bidang veteriner atau yang terkait dengannya. Jurnal Ilmu dan Kesehatan Hewan dikelola oleh Program Studi Magister Kedokteran Hewan Universitas Udayana dan terbit dua kali setahun.
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Articles 39 Documents
Profil Darah Monyet Ekor Panjang (Macaca fascicularis) Liar di Habitat Alami Soma, I Gede; Wandia, I Nengah; Putra, I GA Artha; Silta, Rostiani
Jurnal Ilmu dan Kesehatan Hewan Vol. 1 No. 1 2013
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Study of blood profile of Macaca fascicularis in wild habitat was conducted using 29 blood samples of  adult Macaca fascicularis from Alas Purwo National Park and Baluran National Park, East Java. Blood samples of Ketamine-anaesthetized Macaca fascicularis were taken via femoral vein. Data were divided according to the sex. The result showed that there was sexual dimorphisms of blood profiles of adult Macaca fascicularis. Blood profiles of the adult male were the total erythrocytes count: 4.9 ± 0.4 (x106/µl), hemoglobin titer: 10.9 ± 0.9 g/dl, packed cell volume: 35.5 ± 3.5%, blood sedimentation rate: 1.5 ± 0.2 mm/h, MCH: 20.9 ± 2.1 pg, MCV: 63.8 ± 5.7 fL, MCHC: 28.7 ± 2.5 pg, total leukocytes count: 5777 ± 551.1/µl, limphocytes 23.6 ± 1.6%, neutrophils 57.2 ± 1.7%, eosinophils 9.6 ± 0.5%, and monocytes 9.6 ± 0.6%. Blood profiles of adult female were the total erythrocyte count: 3.9 ± 0.5 (x106/µl), hemoglobin titer: 8.8 ± 1.7 g/dl, packed cell volume: 32.1 ± 4.1%, blood sedimentation rate: 1.3 ± 0.3 mm/h, MCH: 20.5 ± 4.2 pg, MCV: 74.8 ± 9.9 fl, MCHC: 24.1 ± 4.7 pg, total leukocytes count: 5244.4 ± 1017,9/µl, limphocytes: 22.9 ± 1.8%, neutrophils 56.3 ± 1.7%, eosinophils 11.2 ± 0.7%, and monocytes 9.6 ± 0.6%.
Polimorfisme Lokus Mikrosatelit D10S1432 pada Populasi Monyet Ekor Panjang Di Sangeh Rell, F.; Widyastuti, S.K.; Wandia, I N.
Jurnal Ilmu dan Kesehatan Hewan Vol. 1 No. 1 2013
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Genetic polymorphisms are variations in the genetic structure of populations that represents the base level of biodiversities.  This research aimed to assess the polymorphism of microsatellite locus of D10S1432 in the population of long-tailed macaques in Sangeh, Bali.  A total of 18 blood samples collected as a source of DNA. DNA was extracted using QIAmp® DNA Blood Mini Kit. Microsatellite locus was amplified by polymerase chain reaction technique (PCR) with a total of 30 cycles, and a 57oC of annealing temperature. Furthermore, the alleles were separated by 7% polyacrylamide gel electrophoresis, and emerged with silver staining. The results showed that there were three types of alleles found at D10S1432 locus in the long-tailed macaques population in Sangeh. The alleles size varied from 170 bp to 186 bp. The allele frequencies were  0.28, 0.67, and 0.05 for allele 170, allele 174, and allele 186 consecutively. The heterozygosity of the locus was 0.48. It can be concluded that microsatellites locus D10S1432 is polymorphic in the long-tailed macaques population in Sangeh.
Penampilan Reproduksi Sapi Bali pada Peternakan Intensif di Instalasi Pembibitan Pulukan Siswanto, Mahmud; Patmawati, Ni Wayan; Trinayani, Ni Nyoman; Wandia, I Nengah; Puja, I Ketut
Jurnal Ilmu dan Kesehatan Hewan Vol. 1 No. 1 2013
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This studi was conducted to investigate the reproductive performances  of Bali cattle under intensive management in breeding instalation Pulukan Bali from  2008  to  2010.  Totaling 298 2 - 6.5 year old heifers were use to represents the benchmark reproductive performances of Bali cattle. Data collected were age at first heat (AFH), age at first calving (AFC), calving interval (CI), and services per conception (SPC). The overall means for  AFH, AFC,and  CI  were  718.57 ± 12.65; 1104.51 ±  23.82, and 350.46 ± 27.98 days  respectively,  and SPC was 1.65 ± 0.87.
Hubungan Frekuensi dan Waktu Kawin dengan Jumlah Anak Sekelahiran pada Anjing Golden Retriever yang Dipelihara Di Kota Denpasar Oktaviami, Tessa Yuli; Indira Laksmi, Desak Nyoman Dewi; Puja, I Ketut
Jurnal Ilmu dan Kesehatan Hewan Vol. 1 No. 1 2013
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This retrospective study was conducted to know the relation between time and frequency of mating on litter size of Golden Retriver  in Denpasar Region. Mating incident records of 19 Golden Retrievers from 8 kennels were compiled to be analyzed. These data can be use as a reference in effort to maximize the amount of  Golden Retriever clans. The result showed that the three times of mating on the 10th , 12th and 14th day after onset of proestrus produced the most litter size 10 ± 2.82. However, mating twice on wice on 9th and 11th after onset of proestrus produced a litter size of 8.50 ± 0.70. The statistic analysis depicted that there was no significant relationship between mating frequencies and mating time on litter size.
Deteksi Antibodi Mycobacterium tuberculosa bovis pada Sapi di Wilayah Kabupaten Buleleng, Bangli, dan Karangasem Provinsi Bali Putra, Putu Gede Widiarsa; Besung, Nengah Kerta; Mahatmi, Hapsari
Jurnal Ilmu dan Kesehatan Hewan Vol. 1 No. 1 2013
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Bovine tuberculosis (BTB) is one the most widespread zoonotic diseases and has recently reemerged as a major health concern. The disease is caused by the Mycobacterium tuberculosis complex (MTBC) that primarily affects humans as its main host, and that is able to attack more hosts such as domestic animals, pet animals, and wild animals. This Cross-Sectional Study was carried out to determine the seroprevalence of BTB in Districts of Buleleng, Bangli, and Karangasem Province of Bali. One hundred and eighty cow blood samples were collected, and the serological status of BTB was detected using Elisa method. The results showed that the seroprevalence of BTB in District of Bangli was 2.22%, while, those was negative in Districts of Buleleng and Karangasem. The positive seroprevalence of BTB in Bangli indicates that the same research must be conducted throughout the districts in the Province of Bali.
Seleksi Awal Pejantan Sapi Bali Berbasis Uji Performans Patmawati, Ni Wayan; Trinayani, Ni Nyoman; Siswanto, Mahmud; Wandia, I Nengah; Puja, I Ketut
Jurnal Ilmu dan Kesehatan Hewan Vol. 1 No. 1 2013
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Performance test is a preliminary selection to choose Bali cattle stud based on qualitative and quantitative characteristics, including measurement, weighing and observation. Performance test is  applied  on rapid growth of bull  (about 1 year of age) by comparing individual performance with the performance of the group average. The number of bulls that were subjected  the  Performance test  in 2011  was 61  bulls.  Based on Performance test, the best three of the performances  namely the score of 113.30, 109.46,and 104.2  were s selected to be potential stud candidates for next Zuriat test (progeny test).
Kepekaan Eschericia coli Patogen yang Diisolasi dari Babi Penderita Kolibasilosis terhadap Antibiotik di Kecamatan Kerambitan dan Tebanan Kabupaten Tabanan, Bali Nugraha, Aditya; Besung, NK.; Mahatmi, Hapsari
Jurnal Ilmu dan Kesehatan Hewan Vol 1, No 2 (2013)
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This study aims to determine the sensitivity pattern of E. coli isolated from pigs with colibacillosis to antibiotics. The total of 21 fecal samples was collected from pigs with white scour at the District of Kerambitan and Tabanan, the Regency of Tabanan, Bali. The isolation and identification of pathogenic E coli used Sheep Blood Agar medium, and serotype grouping of those bacteria was designated through agglutination test using serum’s antibody Polyvalent 1-5 and 6-11. The pathogenic E. coli found was then examined it`s own resistance to streptomycin, sulfamethoxazole, gentamicin, cefotaxime, and chloramphenicol according to Kirby Bouer method. The result showed that the isolates of E. coli polyvalent 1-5 were resistant to streptomycin and chloramphenicol, whereas that of polyvalent 6-11 was resistant to sulfamethoxazole, gentamicin, streptomycin and chloramphenicol.
Kadar Estrogen dan Munculnya Estrus setelah Pemberian Buserelin (Agonis GnRH) pada Sapi Bali yang Mengalami Anestrus Postpartum Akibat Hipofungsi Ovarium Suartini, Ni Ketut; Trilaksana, I Gusti Ngurah Bagus; Pemayun, Tjok Gde Oka
Jurnal Ilmu dan Kesehatan Hewan Vol 1, No 2 (2013)
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The purpose of this study was to measure the levels of estrogen and the onset of estrous after administration of Buserelin on bali cattle experiencing anestrous postpartum due to ovarian hypofunction. This research used twenty seven bali cattles which were divided evenly into three groups of treatment, namely group-one as control group (P0, without treatment), group-two, receiving a single injection of Buserelin at dose of 0.02 mg (P1) and group three, receiving repeated injection of Buserelin at dose of 0.02 mg at 24 hours interval (P3). Serum estrogen levels were measured at a time off before estrous, a time of estrous, and five days after the onset of estrous using ELISA method. Data were analyzed using statistical analysis of variance and a Duncan’s Multiple Range Test for a further analysis. The results showed that at the stage of estrous, the estrogen level of P0 was lower significantly (p<0.05) than two others treatments (P1 and P2), but those of P1 and P2 were statistically the same (p>0.05). The onset of estrous on P2 was shorter than those of P1 (p<0.05). It can be concluded that the administration of Buserelin can trigger an estrous on Bali cattle that experiencing anestrous postpartum due to ovarian hypofunction and repeated administrations of Buserelin can shorten the onset of estrous.
Bioaktivitas Gel Aloe vera pada Gonad Tikus Putih Jantan (Rattus norvegicus) Suardita, I Ketut; Puja, I Ketut; Pemayun, Tjok Gde Oka
Jurnal Ilmu dan Kesehatan Hewan Vol 1, No 2 (2013)
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The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus). This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD) Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it’has a high possibilities as an alternative herbal contraceptive agent for animals
Resistensi Bakteri Aeromonas sp. Isolat Ikan Piranha (Pygosentrus nattereri) Bali Safari And Marine Park terhadap Antibiotik Herawati, Ni Made; Mahatmi, Hapsari; Besung, I Nengah Kerta
Jurnal Ilmu dan Kesehatan Hewan Vol 1, No 2 (2013)
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Piranha (Pygosentrus nattereri) is a fish that has been conserved at Conservation Center of Bali Safari and Marine Park. In May 2012 there was a case that twelve Piranhas suffered from illness and two of them died without any specific clinical signs. The dead Piranhas were necropsied and the tissues or parts of organs were collected for microbial examination interests. Spesies specific bacterial examination was also included using Microgen GNA TM + B-ID system test The bacterial examination found that the fishes were infected by Aeromonas hydropila and Aeromonas veronii. Further examination about antibiotic resistance tests showed that those Aeromonas spp were resistant to chloramphenicol and a combination of trimethoprim and sulphamethoxazole. It can be suggested that, next, the usage of other antibiotics is strongly recommended for the case of Piranha infected by Aeromonas spp at Bali Safari and Marine Park.

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