cover
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. badung,
Bali
INDONESIA
Journal of Chemistry
Published by Universitas Udayana
ISSN : 19079850     EISSN : 25992740     DOI : -
Jurnal Kimia (Journal of Chemistry) publishes papers on all aspects of fundamental and applied chemistry. The journal is naturally broad in scope, welcomes submissions from across a range of disciplines, and reports both theoretical and experimental studies. As well as original research, Jurnal Kimia (Journal of Chemistry) also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.
Arjuna Subject : -
Articles 324 Documents
FRAKSINASI DAN IDENTIFIKASI SENYAWA TANIN PADA DAUN BELIMBING WULUH (Averrhoa bilimbi L.) Kamilah Hayati, Elok; Fasyah, A. Ghanaim; Sa’adah, Lailis
Journal of Chemistry Vol. 4, No. 2 Juli 2010
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Fractionation of tannic compound from star fruit leaves was conducted by maseration using acetone : water(7:3), and analytical and preparative thin layer chromatography using a variety of eluents. The isolates obtained wereidentified using UV-Vis and FT-IR Spectrophotometer.The best eluent for both analytical and preparative TLC was found to be n-butanol : water : acetic acid(4:1:5), which gave three isolates with Rf of 0.53, 0.61, and 0.68. The second isolate (Rf = 0.61), showed a maximumabsorption at 331 nm on the UV-Vis spectra. Its IR spectra showed specific absorptions such as OH-assymetricstretch (3372.2 cm-1), aromatic overtone (2071.8 cm-1), and aromatic and benzene stretches at 1625.8 cm-1 and 782.5cm-1 respectively. These spectra suggest that the tannic compound was either flavan pent-3,6,7,4,5-ol or flavanpent-3,7,8,4,5-ol.
KARAKTERISASI TOXOPLASMA GONDII ISOLAT INDONESIA Chandra Yowani, Sagung; Kumolosasi, Endang; Singgih Wibowo, Marlia
Journal of Chemistry Vol. 1, No. 1 Januari 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Cathodic protection basically reduces the corrosion rate of a metallic structure by reducing its corrosionpotential, Toxoplasma gondii isolated from diaphragm of sheep at an abbatoir in Sukabumi, West Java had beencharacterized by Centre Research Institute for Animal Sciences. The characterization included study of morphologyby optical microscope, study of ultrastructure by transmission electron microscope, the study of the parasite growthin mice Mus musculus, and study of proteins of the parasite. The growth of parasite in mice had been studied usingtwo groups of mice i.e., normal group and immunosuppressed group. The number of parasites was comparedstatistically using student’ t-pair test. Results showed that dexamethasone at a dose of 5.2 mg/20 g body weight intraperitoneally to the immunosuppresed mice did not increase the number of extracelluler parasites in the peritonealfluid. The best parasite harvest time was on the 4th day after inoculation. Determination of parasite protein obtainedat 4 days after inoculation using sodium dodecyl sulphate polyacrylamide gel electrophoresis showed a dominantsurface protein of 42 kDa.
KARAKTERISTIK ARUS DAN POTENSIAL KATODIK PADA PERLINDUNGAN SISTEM ARUS TERPASANG TERHADAP STAINLESS STEEL TYPE 304 DI LINGKUNGAN AIR LAUT Asmara, Yuli Panca
Journal of Chemistry Vol. 1, No. 1 Januari 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Cathodic protection basically reduces the corrosion rate of a metallic structure by reducing its corrosionpotential, bringing the metal closer to an immune state. So, to optimallize the protection, not only potential but alsoenvironment and system should be considered in designing cathodic protection. The changes of current provided by adirect current (DC) source should be a counted during the protection.The objective of this laboratory was to studed the polarization behaviour concerning 304 stainless steelimmersed in the artificial sweater based on ASTM D 1141 using potentiostat. This task was performed by recordingdata points (potential across a constant resistor) within a determined potential. Then, the surface morphology ofspecimen caused by polarisation potential was investigated using an optical microscope and SEM analysis.The current derived from the polarisation diagram by the determination of several points protectionpotential ranging from -730mV to -1100 mV tend to decrease. The sharp decrease of current occured at protectionpotential of -1100 mV (from 580 mA/m2to 450 mA/m2) there was a slight decrease at protection potential of -730mV. The decrease occurred was from 190 mA/m2 to 150 mA/m2. Potential of protection cathodic also influenced the free corrosion potential. At the protection potential of -730 mV, free corrosion potential was -225 mV, and was-450 mV at the protection potential of -1100mV.By visual examination it can be deduced that the decrease of current density occurred was caused by thegrowth of salt calcareous deposits. By SEM investigation it was indicated that the deposit was made of calcium andmagnesium as a result of the increase of pH during protection. The more detail analisys showed that this depositcreated local pitting corrosion and decreased pitting potential by 200 mV.
PENENTUAN pH OPTIMUM ISOLASI KARAGINAN DARI RUMPUT LAUT JENIS Eucheuma cottonii Bawa, I G. A. G.; Bawa Putra, A. A.; Ratu Laila, Ida
Journal of Chemistry Vol. 1, No. 1 Januari 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

This research aimed to determine the optimum pH for the isolation carrageenan from Eucheuma cottoniiseaweed. The isolation was conducted at various pH : 7.5, 8.0, 8.5, and 9.0. A TLC using methanol : water (5:1) asmobile phase from standard carrageenan, gave result on Rf value 0.74 as equal to sample on treatment condition atpH 8.0 and 8.5. whereas, sample on treatment condition at pH 7.5 and 9.0 gave Rf value 0.72. In using ethanol :water (3:1) as mobile phase gave result on Rf value 0.75 from both standard and sample treated at 7.5, 8.0, and 8.5,while at pH 9.0 gave Rf value 0.74. Melting point test from standard gave range value 240-242, while for sample ontreatment condition at pH 7.5, 8.0, 8.5, and 9.0 gave range value each such 242-244, 241-243, 240-242, and 240-243.Compounds identification with solubility test method using various solvent medium obviously showed that both ofstandard and sample from various treatment condition were insoluble such as in aquades 200C, NaCl 25% 200C and800C, saccharosse 65% 200C and chloroform. Otherwise, they were soluble in aquades and saccharosse 65% withtemperature 800C. Infrared spectrum standard compound and sample from various treatment condition indicatedthat they were identical. Carrageenan isolation produce the largest number of rendemen at pH 8.5 with about34.65%, while the variant of carrageenan isolate was classified by kappa carrageenan.
ANALISIS VARIASI NUKLEOTIDA DAERAH D-LOOP DNA MITOKONDRIA PADA SATU INDIVIDU SUKU BALI NORMAL Ratnayani, Ketut; Wirajana, I Nengah; Laksmiwati, A. A. I. A. M.
Journal of Chemistry Vol. 1, No. 1 Januari 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Human mitochondrial DNA (mtDNA) has higher polimorfism level than nucleous genom, especially in theD-Loop region, which is a non coding region and the most polymorfic region in the mitochondrial genom. Theanalysis of variation of nucleotide sequence of D-Loop region can be used to determine the individual or ethnicidentity and also maternal familiar relationship. The research aims to determine nucleotide variant on Balineseindividue, which can be used as data base in determination of mtDNA genetical profile of Balinese ethnic in a biggerscale.To achieve the aims of the research, way the nucleotide sequence of one normal Balinese individue usingthe epithelia cells in the saliva. The methods were :1) the isolation of sample mtDNA, 2) the amplification of the DLoopregion of mtDNA by PCR, 3) sequencing and analysis of nucleotides sequence.The 0,4 kb fragment of the D-loop region mtDNA of the sample were successfully amplified, andsequenced of 402 pb. The research found 6 new variants or morfe different from Cambridge or Anderson sequence :variant 16223C®T, 16249T®C, 16259C®T, 16278C®T, 16316A®G, 16375C®A. The research also found thedeletion of T nucleotide on position 16362.
ISOLASI SENYAWA SITOTOKSIK DARI DAUN ANDONG (Cordyline terminalis Kunth) Bogoriani, N. W.; Rahayu Santi, Sri; Astiti Asih, I. A. R.
Journal of Chemistry Vol. 1, No. 1 Januari 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Isolation and identification one of cytotoxic fraction have been conducted from the leaves of Andong(Cordyline terminalis Kunth). The fraction having LC50 values of 41,64 ppm contains saponin. Major isolate (7.5 mgwhite amorphous solid) was obtained after a series of chromatographic separations (gravity column chromatography,and preparative high performance liquid chromatography). Identification of the isolate using mass spectrometry withpositive electrospray showed MW of 866 as calculated from the ion peaks m/z 889[M + Na]+, and 867[M + H]+.The ion peaks at m/z 721[(M + H) - 146]+, 703[(M + H) - 164]+, 575[(M + H) - 292]+, 557[(M + H) - 310]+ , 429[(M+ H) - 438]+, and 411 [(M + H) - 456]+ of its fragments indicate the presence of three sugars (two terminal sugarsand one central sugar) from methylpentose moiety with MW 164 eash linked to an agyicone of MW of 428. Protonmagnetic resonance spectrum of the isolate in pyridine-d5 showed characteristic proton signals for three steroidmethyls (two angular methyls and one secondary methyl) at d 1.37 (s), 0.84 (s) and 1.02 (d, J = 6.6 Hz); anexomethylene group at d 4.79 ppm and 4.71 (each br s); an ethylene group at d 5.52 ppm (br d, J = 5.4 Hz); signalsof the protons linked to C26 at d 4.01 and 4.44 ppm (each d, J = 12.0 Hz), and three anomeric protons at d 6.43 ppm(br s); 5.56 ppm (br s) and 4.57 ppm (d, J = 7.0 Hz). From the above data it can be indicated that the isolate resultedis spirostan steroidal saponin.
ISOLASI DAN IDENTIFIKASI SENYAWA TOKSIK PADA SPONS DARI PERAIRAN GILI SULAT-LOMBOK Dira Swantara, I M.; Supriyono, Agus; Trinoviani, Mila
Journal of Chemistry Vol. 1, No. 2 Juli 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The isolation and identification of toxic compound on sponge from BPPT collection sub P3-TFM derivedfrom Gili Sulat, Lombok waters has been conducted. The extraction applied was maceration method, which wastotally conducted using three kinds of solvents namely, methanol, dichloromethane, and n-hexane. The total extractobtained was then partitioned into liquid using ethyl acetate and n-butane respectively. The most toxic extraction wasseparated by chromatography column with sephadex LH-20 as the stationary phase and methanol as the mobilephase. Identification of the active isolate was conducted by gas chromatography - mass spectroscopy with the aim ofWiley 275.L. database.Toxin test to sponge of BPPT collection sub P3-TFM showed that SL2 sponge was proved to be the mosttoxic one. The most toxic fraction of SL2 sponge at LC50 at 100 ppm was assumed to be the combination of 7compounds, namely benzene acetatic acid (8.9%), dioktil hexadioate (4.5%), bis (2etilheksil) 1.2-benzeneboksilat(53.1%), 2, 6, 10, 15, 19, 23 hexamethyl 2, 6, 10, 14, 18, 22-tetracosahexene, (17.1%), nonacosane (7.8%),cholesterol (3.8%) and eicosane (4.8%).
PENENTUAN KUANTITATIF MORFIN DALAM URIN SECARA SPEKTROFOTODENSITOMETRI Suaniti, N. M.; Hitapretiwi Suryadhi, M. A.
Journal of Chemistry Vol. 1, No. 2 Juli 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Quantitative determination of morphine in urine has been done by utilizing spectrophotodensitometry.Morphine standard in methanol 5 g/mL was spotted by linomat between 4-24 L in metal sheet thin layerchromatography spectrophotodensito-metry using morphine standard of 20.40.60.80.100.120. and 140 ng.Methode. statistically and validation analysis, showed a regression line of y = -69.21 + 8.06 x with r =0.992. Detection and quantitation limits of 18.02 ng and 60.06 ng respectively were measured at maximum wavelength of (maks) 287 nm with on recovery of 90.91 percent.
ISOLASI DAN IDENTIFIKASI SENYAWA ANTIMAKAN DARI BATANG TUMBUHAN BROTOWALI (Tinospora tuberculata BEUMEE.) Sukadana, I M.; Susanah Rita, Wiwik; R. Koreh, Frida
Journal of Chemistry Vol. 1, No. 2 Juli 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Isolation and identification of antifeedant compounds from brotowali stem (Tinospora tuberculataBEUMEE.) was done. Brotowali dry powder of 1 kg was extracted using methanol by maceration, The methanolextract then was fractionated repeatedly by n-hexane, so that methanol and n-hexane extracts were obtained. Both ofextracts were evaporated by rotary vacuum evaporator, so concentrated methanol and n-hexane extracts wereobtained and then their antifeedant activity was tested. More active extract was separated by thin layerchromatography (TLC) then continued by column chromatography utilizes silica gel 60 as a stationary phase and thebest mobile phases of TLC. Fractions obtained were tested of their antifeedant activity. More active fraction thenwas tested its purity and identified by phytochemical test and UV-vis and infrared spectrophotometer.As much as 97.07 g of concentrated methanol extract was resulted from 1 kg brotowali dry powder.Fractionation of the methanol extract using n-hexane resulted 22.92 g of concentrated methanol and 21.71 g ofconcentrated n-hexane. n-hexane extract points out more activity as antifeedant than methanol extract. The bestmobile phase of TLC was n-hexane: chloroform (1:1 ). The unite result from the column chromatography wasobtained 3 fractions where fraction c points out antifeedant active. The purity test of fraction c was obtained 1 spotso continued by phytochemical test and UV-vis and infrared spectrophotometer. Phytochemical test andspectrophotometry analysis from active isolate (fraction c) point out that the compound included triterpenoid groupthat absorb on wavelength 288.6 nm and 310.6 nm, and be considered having functional group of O-H bonded, CO,C-H, C=O, C=C, and C-H.
ISOLASI DAN IDENTIFIKASI SENYAWA AKTIF LARVASIDA DARI BIJI MIMBA (Azadirachta indika A. Juss) TERHADAP LARVA NYAMUK DEMAM BERDARAH (Aedes aegypti) Suirta, I W.; Puspawati, N. M; Gumiati, N. K.
Journal of Chemistry Vol. 1, No. 2 Juli 2007
Publisher : Journal of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Isolation and identification of larvicidal active compounds towards Aedes aegypti from Mimba seed havebeen conducted. One kilogram dry powder of Mimba seed was extracted with ethanol at room temperature.Evaporation of ethanol gave 30 g of crude ethanol extract which showed activity against Aedes aegypti (LC50282.29). This extract was dissolved into methanol-water (7:3) and was then partitioned with n-hexane, chloroformand ethyl acetate respectively. The three extracts obtained i.e. n-hexane, chloroform and ethyl acetate were showedtheir activity against Aedes aegypti in which the-n-hexane extract was the most active with LC50 of 143.97.Therefore the n-hexane extract was further purified using silica gel column chromatography with chloroform:nhexane(9:1) as eluent. Three fractions was obtained i.e. F1, F2 and F3 and they were all active against Aedes aegyptiwith LC50 78.45, LC50 113.54 and LC50 58.70 respectively. It can be seen that F3 was the most active fractions butfrom TLC result, F1 showed the relatively pure compounds since it only gave one spot. Therefore F1 was furtheridentified using pyhtochemical testing, Uv-Vis, infrared and GC-MS. It was found that the larvicidal activecompounds F1 was belong to carboxylic acids groups,with l max 290.1 having functional groups such as methyl,methylene and carbonil. Identification using GC-MS indicated that the larvicidal active compounds F1 was assumedto be a combination of 7 compounds derived from carboxylic acids including hexa-decanoic acid, etil-hexadecanoate,oleic acid, etyl-oleate ester, octadecanoic acid, etyl-octadecanoate and dioctyl-hexadioate.

Page 1 of 33 | Total Record : 324