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INDONESIA
INDONESIAN JOURNAL OF PHARMACY
ISSN : 23389427     EISSN : 23389486     DOI : -
Core Subject : Health,
Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.
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Articles 6 Documents
Search results for , issue " Vol 29 No 4, 2018" : 6 Documents clear
Efficacy of Thymol and Eugenol Against Polymicrobial Biofilm Hamzah, Hasyrul; Tunjung Pratiwi, Sylvia Utami; Hertiani, Triana
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1354.744 KB) | DOI: 10.14499/indonesianjpharm29iss4pp214

Abstract

Biofilms associated with human infection have high levels of pathogenicity due to their resistance to antibiotics. The discovery of an active antibiofilm agent against polymicrobial biofilms is a necessary consequence for coping with biofilm-related infections. Thymol and Eugenol are essential oils that have potential as antibacterial and antifungal. This study aimed to determine the effectiveness of thymol and eugenol inhibits C. albicans, P. Aeruginosa, E. coli S. aureus and polymicrobial biofilm. Biofilm formation inhibition assay and biofilm degradation assay of thymol and eugenol were determined using microtiter broth method.The antibiofilm efficacy of thymol and eugenol towards polymicrobial biofilms were analyzed by calculating minimum biofilm inhibitor concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The data were analyzed using Statistical Package for the Social Sciences (SPSS) with 95% confidence level. Thymol and eugenol showed inhibitory activity against the formation of mono and polymicrobial biofilms of the microbial tested.The result also demonstrated an evidence of activity of thymol and eugenol in breaking down mono and polymicrobial biofilm. Therefore, thymol and eugenol serves as a potential source for new antibiofilm drugs towards polymicrobial biofilm.
α-Glucosidase Inhibition Activity of Stem Bark Ceiba pentandra Linn. In Vitro and In Silico Syihabudin, Vicri
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1718.264 KB) | DOI: 10.14499/indonesianjpharm29iss4pp206

Abstract

Ceiba pentandra (L.) stem bark plant contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. It is known that flavonoid compounds have a potential for inhibition activity of an α-glucosidase enzyme. The purpose of this research is to know the α-glucosidase inhibition activity of the fraction of n-hexane, ethyl acetate, and water. These three fractions measured α-glucosidase inhibition activity by spectrophotometric UV-vis method. The fraction extract with IC50 values can best be done an analysis of active compounds using LCMS/MS methods, and results of the suspected compound do the binding molecule with a receptor model isomaltase α-glucosidase uses AutoDock Vina so that bond can predict drug active compounds. The results of this research activity are inhibition of α-glucosidase stem bark in the fraction of n-hexane 4,60 μg/mL, ethyl acetate fraction 8,55 μg/mL and fractions of the water 5,61 μg/mL. Analysis of the compound fraction of water by LCMS/MS eluted at the retention time 3,61 seconds allegedly derivative compounds (+) catechin and on retention time 8,70 minutes allegedly derivative compounds vavain. Vavain derivative compounds, (+) catechin, acarbose and quercetin each has its own docking score of -8,1; -8,8; -6,2 and -7,6 kcal/mol and has a semblance of bonding on amino acid Glu276 which is a residue catalytic role in hydrolysis reactions. Equality on the sides of active chemical bonds is expected to have the same activity as drug inhibition of the enzyme α-glucosidase.
Screening of Antibacterial and Anticancer Activity of Soft Corals from Togean Islands, Indonesia Zubair, Muhammad Sulaiman; Lallo, Subehan; Rusmiyanti, Rusmiyanti; Nugrahani, Arsa Wahyu; Jantan, Ibrahim
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1388.045 KB) | DOI: 10.14499/indonesianjpharm29iss4pp173

Abstract

Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay agains. Identification for the presence of terpenoids was performed by vacuum p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition range from 12.76mm and 17.86mm, respectively. Moreover, Sinularia sp2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively.  Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp1 and Sinularia sp 2. Terpenoids were identified on both  extracts suggesting for further purification and isolation for the bioactive terpenoid compounds. 
Synthesis and Molecular Docking Studies of N’-benzoylsalicylhydrazide derivatives as antituberculosis through InHA enzym inhibition Santosa, Harry; Putra, Galih Satrio; Yuniarta, Tegar Achsendo; Budiati, Tutuk
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1120.908 KB) | DOI: 10.14499/indonesianjpharm29iss4pp198

Abstract

The specific aims of this study is to synthesize and to study the possible mechanism of N’-benzoylsalicylhydrazide derivatives as an antituberculosis agent through InhA (Enoyl acyl carrier protein reductase) inhibition using in silico method. Five analogues of N’-benzoylsalicylhydrazide were synthesized using microwave irradiation from methyl salicylate as starting material, which yielded 80-90% product on average. This indicates a considerable improvement in terms of effectivity and efficiency, compared to the more conventional method using reflux condition. Character-ization of the compounds were subsequently carried out by UV, FTIR, 1H-NMR, 13C-NMR spectroscopy, which confirmed that the compounds had been successfully synthesized. Ultimately, molecular docking was performed using Molegro Virtual Docker (MVD) on the active site of InhA enzyme to predict the activity of the compounds. The results showed that all compounds performed comparatively well against N-(4-Methylbenzoyl)-4-benzylpiperidine as the native ligand and also yielded lower docking score than isoniazide (INH). From this study it can be concluded that N’-benzoylsalicylhydrazide derivatives could be synthesized using microwave irradiation with good product yield and all of the synthesized analogues are suggested to possess antituberculosis activity via InhA enzyme inhibition. In vitro activity will have to be determined in the future to validate whether N’-benzoylsalicylhydrazide derivatives perform well as a potential antituberculosis agent.
Effects of Antioxidant, Anti-Collagenase, Anti-Elastase, Anti-Tyrosinase of The Extract and Fraction From Turbinaria decurrens Bory. Nurrochmad, Arief; Wirasti, Wirasti; Dirman, Arifin; Lukitaningsih, Endang; Rahmawati, Adillah; Fakhrudin, Nanang
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1362.529 KB) | DOI: 10.14499/indonesianjpharm29iss4pp188

Abstract

Brown macroalgae (BM) which contain fucoxanthin exhibited high antioxidant activity. This study was performed to examine antioxidant, anti-collagenase, anti-elastase, anti-tyrosinase activities, and effect on cell viability of Human Dermal Fibroblast adult (HDFa) of BM, Turbinaria decurrens Bory. T. decurrens dried powder were macerated by ethanol to obtain extract (ETD) and was fractination by column chromatography to obtain fraction (FTD). Fucoxanthin content was determined using HPLC. The antioxidant activities, anti-collagenase, anti-elastase, and tyrosinase inhibitory assay were performed. The effect of ETD and fucoxanthin standard on cell viability were conducted on HDFa cell-induced by hydrogen peroxide (H2O2). The HPLC analysis showed that ETD and FTD contain fucoxanthin of 284.9±3.3µg/g and of 653.4±30.6µg/g dry-weight, respectively. The antioxidant assay showed that ETD and FTD produced high antioxidant activity by ferric reducing antioxidant power (FRAP) and b-carotene bleaching (BCB) methods that were comparable to fucoxanthin. ETD exhibited significantly higher tyrosinase inhibitory than kojic acid (p<0.01), while FTD had a comparable effect to kojic acid. The result also revealed that ETD and FTD produced anti-elastase and anti-collagenase (matrix metalloproteinase-1 (MMP-1). Fucoxanthin and ETD were able to maintain cell viability on HDFa cell-induced H2O2. This study suggests that T. decurrens may be effective to prevent skin aging and wrinkle formation, possibly through the antioxidant activity and maintain cell viability of fibroblast. 
Cytotoxicity of Tetrahydropentagamavunon-0 (THPGV)-0 and Tetrahydropentagamavunon-1 (THPGV-1) in Several Cancer Cell Lines Ikawati, Muthi; Purwanto, Heri; Imaniyyati, Niar Nurul; Afifah, Anis; Sagiyo, Marrita Langgeng; Yohanes, Jasson; Sismindari, Sismindari; Ritmaleni, Ritmaleni
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1198.508 KB) | DOI: 10.14499/indonesianjpharm29iss4pp179

Abstract

Tetrahydropentagamavunon-0 (THPGV-0) and Tetrahydro-pentagamavunon-1 (THPGV-1), are analogs of a curcumin metabolite, tetrahydrocurcumin, and a derivate of Pentagama-vunon-0 (PGV-0) and Pentagamavunon-1 (PGV-1), respectively.  THPGV-0 and THPGV-1 have been successfully synthesized and are investigated for their anticancer potency.  Cytotoxic assays were performed toward several cancer cell lines to determine values of the IC50 against those cell lines. Assessing cytotoxicity in Vero normal cell line showed the selectivity of those compound.  THPGV-1 showed highest cytotoxic activity in lymphoma Raji cells, a suspension cell line, with an IC50 of 180mM.  Both THPGV-0 and THPGV-1 showed similar potencies in T47D breast cancer cell line with IC50 values of 250-270mM.  Regardless their high selectivity, however, cytotoxic activities of THPGV-0 and THPGV-1 were lower compared to PGV-0 and PGV-1 in HeLa cervical, T47D breast, and WiDr colon cancer cell lines.  Further study using different types of cancer cell lines and confirmation of cell viability by another assays and apoptosis detection may give more benefit. 

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