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INDONESIA
INDONESIAN JOURNAL OF PHARMACY
ISSN : 23389427     EISSN : 23389486     DOI : -
Core Subject : Health,
Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.
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Articles 8 Documents
Search results for , issue " Vol 23 No 3, 2012" : 8 Documents clear
DEVELOPMENT AND EVALUATION OF IN SITU GELS OF MOXIFLOXACIN FOR THE TREATMENT OF PERIODONTITIS Kunche, Hareesh Babu; Ahmed, Mohammed Gulzar; Rompicharla, Narayana Charyulu
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (810.129 KB) | DOI: 10.14499/indonesianjpharm0iss0pp141-146

Abstract

Gel  dosage  forms  are  successfully  used  as  drug  delivery systems  to  control  drug  release  and  protect  the  medicaments from  a  hostile  environment.  The  main  objective  of  the  present investigation is to formulate and evaluate in situ gels of moxifloxacin  for  the  treatment  of  periodontitis  using  gellan  gum  and sodium  alginate  based  on  the  concept  of  ion  activated  systems. The  system  utilizes  polymers  that  exhibit  sol-to-gel  phase transition due to change in specific physico-chemical parameters. Sol-to-gel transformation occurred in the presence of monovalent/divalent cations. It was found that increase in the concentration of  calcium  ions  produced  stronger  gels.  Formulations  were evaluated for gelling capacity, drug content, clarity, viscosity, gel strength,  spread  ability,  microbiological  studies  and in  vitro release. The results shown by the above characterization studies were  found  to  be  satisfactory.  Experimental  part  showed  that viscosity of  sols  and  gel  strength  was  increased  with  increase  in the concentration of polymers and the sustained release  of drug was observed.  The formulations were therapeutically efficacious, sterile  and  provided  sustained  release  of  the  drug  over  a  period of  time.  These  results  demonstrated  that  the  developed  system is an alternative to conventional drug delivery systems, provides patient compliance and economical.Key  words:  in  situ  gels,  moxifloxacin,  periodontitis,  polymers,  Ion activation.
ATTEMPTED SYNTHESIS OF BIS-SPIROEPOXIDE DITHIANEDIOXIDE Ritmaleni, Ritmaleni; Aggarwal, Varinder K.
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (812.426 KB) | DOI: 10.14499/indonesianjpharm0iss0pp188-192

Abstract

The  bis-spiroepoxide  dithiane  dioxide  has  been attempted  to  be  synthesised  as  its  role  as  an  intermediate  in the  synthesis  of  diaminopimelic  acid  (DAP).  The  first  method was  carried  out  by  reacting  the  3-dithiane-2-diethylphosphonate  4  with  an  aqueous  solution  of  the commercially  available  glutaraldehide  resulting  the  bis-ketene dithiane  dioxide  5.  The  second  alternative  method  was involving  the  ozonolysis  of  cyclopentene   7  in  the  synthesis  of bis-ketene dithiane dioxide  5 in  four step reactions  which gave moderate to good yield. Unfortunately, epoxidation process  for the bis-ketene dithiane dioxide 5 was still unsuccess yet.Key  words:  synthesis,  diaminopimelic  acid,  bis-spiroepoxide  dithiane dioxide
ELECTROCHEMICAL ANALYSIS OF ZINECARD IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES Reddy, Chennupalle Nageswara; Prasad, Puthalapattu Reddy; Sreedhar, Neelam Yugandhar
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1005.682 KB) | DOI: 10.14499/indonesianjpharm0iss0pp147-155

Abstract

Zinecard is used to prevent a toxic effect to heart caused by certain medicines that are used to treat cancer.  Zinecard  is also  used  to  treat  tissue  damage  caused  by  the  leakage  of certain  medicines  that  are  used  to  treat  cancer.  Differential pulse  polarographic  method   developed  for  the  quantitative determination  of  zinecard  gives  a  peak  at  -0.32  V  at  DME. From  the  structural  point  of  view  zinecard  contains  a  >C=Omoiety  which  can  be  electrochemically  reduced  at  universal buffer  (pH  4.0).  Millicoulometric  experiment  is  performed successfully  in  estimating  the  number  of  electrons  and  proton to  understand  reduction  mechanism.  The  differential  pulse polarographic peak was adequately well-resolved, reproducible and  linear  dependent  with  the  zinecard  concentration.  For quantification  the  calibration  plot   for  zinecard  concentrations ranging  between  1.0×10-5mol  dm-3 to  1.0×10-8mol  dm-3 at pH  4.0  was  selected.  The  proposed  differential  pulse polarographic  method  was  successfully  applied  to  the determination  of  zinecard  in  pharmaceutical  formulations  and urine samples.Key words:  Zinecard,    DPP,    pharmaceutical    formulations    and urine samples. 
Satyrium nepalense: A RARE MEDICINAL ORCHID OF WESTERN HIMALAYA (INDIA); PHYTOCHEMICAL SCREENING, ANTIMICROBIAL EVALUATION AND CONSERVATION STUDIES Mishra, Abhay Prakash; Saklani, Sarla
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (997.941 KB) | DOI: 10.14499/indonesianjpharm0iss0pp162-170

Abstract

Satyrium  nepalense  is  an  endangered  medicinal  herb  found at  the higher altitude of 2400-5000m. Local inhabitant used tubers of  Satyrium  nepalense  as  an  energetic  tonic  and  as  an  important medicine  to  cure  different  type  of  fever  in  traditional  health  care system  of  Uttarakhand  (INDIA).  Present  study  was  carried  out  to evaluate  the  phytochemical,  antibacterial  screening  against  four (Streptococcus  mutans,  Pseudomonas  aeruginosa,  Staphylococcus aureus  and  Klebsiella  pneumoniae)  microorganisms  in  methanolic extracts  and  habitat  studies  for  conservation  of  S.  nepalense. Tubers  of  Satyrium  nepalense  were  extracted  separately  with methanol  by  hot  extraction  process  using  soxhlet  apparatus.  The extracts  were  subjected  to  Lyophilization  to  get  dry  extract  and preserved  in  aseptic  condition.  The  different  group  reagents  used for  phytochemical  screening  as  dragendorffs’s  for  alkaloid, molisch’s  test  for  carbohydrates,  shinoda  test  for  flavonoids  etc. Antibacterial  study  was  carried  out  by  disc  diffusion  method.  The highest  zone  of  inhibition  was  recorded  as  15.0±0.00  mm  against Klebsiella  pneumonia  and  15.0±0.82  mm  against  Staphylococcus aureus.  Phytochemical  screening  shows  the  presence  of  alkaloids, carbohydrates/glycosides,  flavonoids  and  unsaturated  sterols/triterpenes  in  Satyrium  nepalense.  The  result  indicates  that methanolic  extract  of  Satyrium  nepalense  shows  potent antibacterial activity against all four bacterial strains.Key  words:  Uttarakhand,  Satyrium  nepalense,  medicinal  orchid,  antibacterial, phytochemicals
ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM Sesamum indicum Sharma, Priyanka; Sarin, Renu
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.07 KB) | DOI: 10.14499/indonesianjpharm0iss0pp135-140

Abstract

Natural  substances  have  long  served  as  sources  of therapeutic  drugs.  Many  substances  have  been  derived  from traditional medicines. The plants are rich in secondary metabolites. The medicinal properties of these plants have been attributed to the biochemicals present in the plant materials. In addition to their role in  human  and  animal  nutrition,  knowledge  of  micronutrients  and phytochemical  composition  is  fundamental  to  the  understanding  of modes and mechanisms of action of medicinal plants in general. In the  present  investigation,  quercetin  and  kaempferol  have  been isolated  and  identified  from  stem,  leaves  and  unorganized  cultures of  Sesamum  indicum  and  maintained  by  frequent  subculturings  on Murashige  and  Skoog’s  medium  (1962)  supplemented  with NAA+BAP(5.0+0.5mg/L).  The study showed that maximum content of quercetin and kaempferol was observed in 6 weeks old calli and minimum  in  stem  of  S.  indicum.  The  structure  of  the  isolated compound  was  established  on  the  basis  of  physical,  chemical  test and spectroscopic evidences.Key words: Flavonoids,  quercetin,  kaempferol, Sesamum indicum
QSAR MODELING OF 2-[CH(OH)X]-5,8-(OY)2 -1,4-NAPHTHOQUININES AGAINST L1210 CELLS USING MULTIPLE LINEAR REGRESSION ., Ajeet; Singh, Brajpal; Kumar, Vipul
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (939.863 KB) | DOI: 10.14499/indonesianjpharm0iss0pp171-176

Abstract

Quinones are present in many drugs such as anthracyclines, daunorubicin,  doxorubicin,  mitomycin,  mitoxantrones  and saintopin, which are used clinically in the therapy of solid cancers. The  cytotoxic  effects  of  these  quinone  are  mainly  due  to  the inhibition  of  DNA  topoisomerase-II.  It  is  the  necessity  to  develop the  1,4-Naphthoquinone  analogues  with  Cytotoxic  effect.  Here  2-[CH(OH)X]-5,8-(OY)2-1,4-Naphthoquinines  analogues  have  been used  to  correlate  the  cytotoxic  activity  with  the  Eccentric Connectivity index (ECI), Fragment Complexity (FC) and McGowan Volumes  (MG)  for  studying  the  Quantitative  Structure  Activity Relationship  (QSAR).  Correlation  may  be  an  adequate  predictive model  which  can  help  to  provide  guidance  in  designing  and subsequently  yielding  greatly  specific  compounds  that  may  have reduced  side  effects  and  improved  pharmacological  activities.  We have  used  Multiple  Linear  Regression  (MLR),  one  of  the  best methods for developing the  QSAR model. Results from this QSAR study  have  suggested  that  ECI,  FC  and  MG  are  the  important descriptors for cytotoxic activities of 1,4-Naphthoquinones against L1210  cells.  For  the  validation  of  the  developed  QSAR  model, statistical  analysis  such  as  data  point-descriptor  ratio,  fraction  of variance,  cross  validation  test,  standard  deviation,  quality  factor, Fischer’s test; and internal validation such as Y-randomization test have been performed and all the tests validated this QSAR model.Key  words:  1,4-Naphthoquinones,  QSAR,  Eccentric  connectivity  index, Fragment  complexity,  McGowan  Volume,  Multiple  Linear Regression 
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS Septisetyani, Endah Puji; Rubiyana, Yana; Wisnuwardhani, Popi Hadi; Wardiana, Andri; Santoso, Adi
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.905 KB) | DOI: 10.14499/indonesianjpharm0iss0pp177-182

Abstract

Stability  of  erythropoietin  (EPO)  depends  on  its glycosylation  states.  With  more  glycosylation  sites,  the  EPO protein  will  be  more  stable  and  also  increase  its  half-life.  A construct  of  recombinant  human  erythropoietin  (rhEPO)  which contains 2 additional N-link for glycosylation were designed. Based on translation analysis using ORF (open reading frame)-finder and protein  alignment  analysis  using  blast-p  of  NCBI  home  page, expected  recombinant  hEPO  with  additional  6-histidin  tag  in carboxyl terminus  was expressed. HEK293T cells  were transfected with  recombinant  plasmid  containing  rhEPO  by  using  calcium phosphate method. Expression of rhEPO was detected by dot blot and  Western  blot  analysis  using  hEPO  antibody  as  the  primary antibody  and  antirabbit  antibody  with  alkaline  phospatase  linked as  the  secondary  antibody.  The  bands  were  detected  by BCIP/NBT color  development  substrate.  The  data  indicated detection of EPO in culture medium of transfected HEK293T cells.Key  words:  HEK293T  cell,    calcium    phosphate    transfection,  N-linked glycosylation, recombinant human erythropoietin
A BIOAVAILABILITY STUDY OF INDONESIAN GENERIC TABLET OF CAPTOPRIL IN HEALTHY VOLUNTEERS Nugroho, Agung Endro; Hakim, Arief Rahman; ., Purwantiningsih; Hakim, Lukman
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 3, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.336 KB) | DOI: 10.14499/indonesianjpharm0iss0pp183-187

Abstract

Captopril  is  a  selective  inhibitor  of  angiotensin-converting enzyme  (ACE)  and  is  formulated  by  several  pharmaceutical companies in Indonesia. This study was conducted to compare the bioavailability  of  a  captopril  tablet  with  reference  products  in healthy  volunteers.  The  relative  bioavailability  of  captopril  was determined in single dose, randomized, crossover,  and  two-phase studies.  The  relative  bioavailability  of  the  test  product  (a  generic captopril 50 mg tablet) with respect to the reference product was determined. Twelve healthy volunteers in two groups took part in these  studies  and  took  either  the  test  or  reference  tablets  in  the first  phase  and  received  the  other  tablet  in  the  second  phase  of each  study.  The  bioavailability  parameters  include  the  peak concentration  of  captopril  in  serum  (Cmax);  the  time  to  achieve the  peak  concentration  (Tmax);  and  the  area  under  the  curve  of captopril  in  serum  versus  time.  Non-compartmental  analysis  on observed concentration versus time data has resulted in the mean value of Cmax of 545.26 ± 22.90 ng/mL (test product) and 548.91 ± 25.07 ng/mL (reference product) and mean Tmax of 1.13 ± 0.08 hours  (test  product)  and  1.08  ± 0.08  hours  (reference  product), mean  of  AUC0-7  value  of  1820.51  ± 75.31  ng.  hour/mL  (test product)  and  1822.09  ± 99.29  ng.  hour/mL  (reference  product), and  mean  of  AUC0-inf  value  of 1967.83  ±  95.65  ng. hour/mL  (test product)  and  1996.94  ± 124.52  ng.  hour/mL  (reference  product). Based on the data, it can be concluded that there is no significant difference  (p>0.05)  in  bioavailability  between  both  captopril Tablet (test and reference product).Key words: Bioequivalence, Captopril, HPLC, Human serum, Generic 

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