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INDONESIA
INDONESIAN JOURNAL OF PHARMACY
ISSN : 23389427     EISSN : 23389486     DOI : -
Core Subject : Health,
Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.
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Articles 9 Documents
Search results for , issue " Vol 23 No 1, 2012" : 9 Documents clear
PREDICTORS OF THE USE OF GASTROPROTECTIVE AGENTS IN PATIENTS USING NSAID IN YOGYAKARTA INDONESIA Indrianto, Ady Bagus; Thobari, Jarir At; Nugroho, Agung Endro
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.903 KB) | DOI: 10.14499/indonesianjpharm0iss0pp41-47

Abstract

Non Steroid Anti-Inflammatory (NSAID) is one type of AntiInflammatory  drug  that  is  used  to  treat  pain  in  patients  of osteoarthritis  (OA)  and  rheumatoid  arthritis  (RA).  The  use  of NSAIDs can cause gastrointestinal side effects. To prevent the side effects,  NSAIDs  are  prescribed  in  combination  with gastroprotective  agent  (GPA).  The  aim  of  the  study  is  to determine  the  factors  considered  in  the  GPA  prescription  by  the doctors  in  patients  who  have risk  factors  for  gastric  disorder  due to  NSAIDs  use.  This  study was  performed using  a  cross-sectional observational  design.  The  data  were  collected  retrospectively during  a  period  of  January to December  2010.  The subjects were OA  and  RA  patients  considering  the  inclusion  and  exclusion criteria.  The  data  included  patient  characteristics  (gender,  age, occupation, payment  method, the primary diagnosis),  prescription of NSAID, prescription of GPA, history of gastrointestinal disease, NSAID  prescription  with  corticosteroids,  prescription  with antiplatelet.  The  data  were  processed  descriptively  and quantitatively, and analyzed statistically using chi-square and log. regression with 95% Confidence Interval. The results showed that there  were  correlation  between  some  predictor  factors  with prescription  of  gastroprotective agent.  These  factors  were  women patient,  patient  age of  ≥  65  years,  oxicam  prescribing, diclofenac sodium prescribing, prescribing >1 type of NSAIDs, prescription of NSAID  concomitant  with  corticosteroids,  prescription  of  NSAID concomitant  with  antiplatelet,  patients  with  history  of gastrointestinal, prescribing NSAIDs with duration of ≥ 3 months. Patient  with  a  history  of  gastrointestinal  disease  was  the  most predictor  influential  factor,  with  OR  (odds  ratio)  of  3.6  (95%  CI: 2.79  -  4.66).  Predictor  factor  of  patients  with  a  history  of dyspepsia  possessed  the  highest  OR  {OR=4,29  (CI  95%:  3.23–5.7)}.  It  means  that  patients  with  a  history  of  dyspepsia prescribed  NSAIDs  would  have  greater  risk  of  4.29  times  to  get GPA  prescription  than  patients  without  a  history  of  dyspepsia. Prescribing  NSAIDs  >  1  DDD  (defined  daily  dose)  had  p  value  of 0.777,  which  means  there  was  no  relationship  between  GPA prescription with NSAIDs > 1 DDD prescription.Key  words: Gastroprotective  Agent,  Non-steroid  anti-inflammatory, Osteoarthritis, Rheumatoid Arthritis
ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF RED Psidium guajavaL. LEAVES GROWN IN MANOKO, LEMBANG - INDONESIA Fidrianny, Irda; Hartanti, Rika; Raveendaran, Narmmatha
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.492 KB) | DOI: 10.14499/indonesianjpharm0iss0pp36-40

Abstract

Psidium  guajava L.  (Myrtaceae)  is  a  well  known  plant  in Malaysia  and  Indonesia.  Its  leaves  extract  was  found  to  possess antidiarrhea,  antimicrobial,  hepatoprotective  and  antioxidant activities.  Objective  of  this  research  is  to  isolate  an  antioxidant subtance   from  Red Psidium  guajava L.  leaves.  The  crude  leaves was extracted using Soxhlet apparatus by gradual polarity of three different  solvents,  n-hexane,  ethyl  acetate  and  methanol. Antioxidant  activity  of  each  extract  was  tested  by  using  DPPH (2,2-diphenyl-1-picrylhydrazyl)  radical  scavenging  method.  Total phenol,  total  flavonoid  and  total  tannin  content  of  the  extracts were  also  measured.  Ethyl  acetate extract  was fractionated  using vacuum  liquid  chromato-graphy  for  fractionation.  Purification  was performed using TLC  preparative. Isolate then characterized using specific  spray  reagent,  UV-Vis  spectrophotometry  and  infrared spectrophotometry.  Crude  drug  of  Psidiumguajava contained flavonoid,  tannin,  quinone,  saponin  and  steroid/  triterpenoid. Antioxidant  activity  of  ethyl  acetate  extract  is  65.63%  with  total phenol  4.25%,  total  flavonoid  0.53%  and  total  tannin  1.16%. Antioxidant  compound N was  isolated  from  ethyl  acetate  extract.Antioxidant  compound N was  supposed  to  be   aglycone  flavone that has OH at C -4’, C-5 and C-7.Key words: Psidium guajava L. , antioxidant activity, ethyl acetate extract
FORMULATION OF NANOCURCUMIN USING LOW VISCOSITY CHITOSAN POLYMER AND ITS CELLULAR UPTAKE STUDY INTO T47D CELLS Chabib, Lutfi; Martien, Ronny; Ismail, Hilda
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.121 KB) | DOI: 10.14499/indonesianjpharm0iss0pp27-35

Abstract

Using  of  curcumin  as  anti  cancer  agent  is  restricted  by  its low  solubility,  therefore  it  has  low  bioavability.  This  obstacle  can be  solved  by  the  development  of  curcumin  nanoparticle. Nanoparticle  technology  has  been  started  to  be  developed  as  an alternative  solution to  improve drug  delivery pofile, especially  for the less bio-available chemical. This study was aimed to develope nanocurcumin  formulation  with  low  viscosity  chitosan  as  the matrix  and  to  study  its  ability  to  be  taken  into  the cells in  vitro. Method  used  in  the  formulation  of  nanocurcumin  in  this  study  is by ionic gelation followed by freeze drying. Entrapment  Efficiency then  assayed,  and  its  stability  was  tested  by  incubating  the formula  into  artificial  intestinal  fluid  (AIF).  Furthermore,  its toxicity  was  evaluated,  also  its  cellular  uptake  ability  into  T47D cell  line.  It  was  found  that  the  Entrapment  Efficiency  in  acetate buffer  at  pH  4  is  higher  than  at  pH  5.  This  formula  also  has  a good  stability  in  AIF.  For  the  cellular  uptake  study  through fluorescence  microscope,  it  was  found  that  the  complex  has  an ability  to  penetrate  cellular  membrane  into  the  cytosol.  The cytotoxicity  study  tell  us  that  the  nanocurcumin  is  non-toxic  to normal  cell line. For  the characterization of the nanoparticles, the average  size  of  this  particle  is  269.8  nm,  its  zeta-potential  is +18.63 mV, with spherical particle morphology. From the result ofthis study, it is concluded that formulation of nanocurcumin using low viscosity chitosan polymer as the matrix has a great potential as an alternative for anticancer therapy.Key words: nanoparticle, curcumin, low viscosity chitosan, T47D cell line. 
HEPATOPROTECTIVE EFFECT OF GAMAVUTON-0 AGAINST D˗GALACTOSAMINE/LIPOPOLYSACCHARIDE-INDUCED FULMINANT HEPATIC FAILURE Nurrochmad, Arief; Sari, Ika Puspita; Murwanti, Retno; ., Sardjiman; Candraningrum, Triana; Afritasari, Dyah; Martina, Devina; Siahaan, Iren Wati
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.554 KB) | DOI: 10.14499/indonesianjpharm0iss0pp18-26

Abstract

The  objective  of  this  study  is  to  determine  the  hepatoprotective  effect  of  GVT-0  (one  of  curcumin  analogues)  against liver  damage  in  rat-induced  D-galactosamine  (D-GalN)/lipopolysaccharide  (LPS)  as  a model  of fulminant  hepatitis.  In  the study D˗GalN/LPS  elevated  serum  GPT  activity  that  indicate  a particular occurrence of liver damage due to depletion of UTP and UDP-glucuronic acid. Administration of GVT-0 (10 mg/kg) showed decreased  enzyme  activity  of  SGPT/SGOT  but  had  no  effect  on serum  ALP  and  total  bilirubin  levels,  whereas  at  doses  of  20 and 40  mg/kg,  the  protective  effect  of  GVT-0  was  decrease.  The glutathione  content  in  the D-GalN/LPS  (0.76  ±  0.07)  mol/g  liver content was found lower than  controls  (0.90  ±  0.03)  mol/g  liver. Administration  of  GVT-0  dose  of  10,  20  and  40  mg/kg  restored glutathione content returned  to normal  levels. The results showed that treatment of GVT-0 showed no effect on TBARS and catalase activity.  Treatment  of  D-GalN/LPS,  indicating  the  trend  of increased  TNF-α,  although  statistically  not  significant,  while  the administration  of  GVT-0  showed  a  tendency  to  decrease  the concentration  of  TNF-α.  All  findings  of  the  results  indicated  that the GVT-0 mainly lower dose (10 mg/kg) showed hepatoprotective action  in rat  model  of fulminant  hepatitis induced  by D-GalN/LPS. The  results  indicated  that  the  mechanism  of  hepatoprotective effect  of  GVT-0  is  not  via  antioxidant  properties  of  GVT-0. However,  further  studies  are  necessary  to  explain  the  molecular mechanism of hepatoprotective effect of GVT-0.Key  words:  Gamavuton-0,  hepatoprotective,  fulminan  hepatitis, D˗galactosamine/LPS 
SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL Handayani, Sri; Risdian, Chandra; Meiyanto, Edy; Udin, Zalinar; Andriyani, Rina; Angelina, Marissa
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.839 KB) | DOI: 10.14499/indonesianjpharm0iss0pp48-53

Abstract

Apoptosis  is  an  important  target  on  anticancer  mechanism. The  purpose  of  this  research  is  to  investigate  apoptosis  induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol  was  used  to  extract Selaginella  plana powders.  Ethanolic extract  was  dilluted  by  methanol:water  (4:1)  and  then fractionated  by  hexane  (S_Hex),  methylene  chloride  (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D  cell  line  was  detected  by  SRB  (Sulforhodamine  B) assay which  was  measured  at  a  wavelength  of  515nm.  Flowcytometry analysis  to  determine  apoptosis  was  examined  by  Propidium Iodide  (PI)  and  Annexin  V  assay  using  T47D  breast  cancer  cell line.  The  result  showed  that  the  IC50 value  of  S_Hex,  S_MTC, S_EA,  and  S_BuOH  on  T47D  cells  were  107  µg/mL,  4  µg/mL,  6 µg/mL,  and  17  µg/mL  respectively.  The  active  fractions  (S_MTC and  S_EA)  at  its  IC50 concentration  significantly  (P<0.05) increased  the  total  number  of  early  apoptotic  cells  in  the  T47D cells  3.39%  and  4.1%  respectively  compared  to  that  of  control (1.95%).  Based  on  the  result,  methylene  chloride  and  ethyl acetate  fraction  of Selaginella  plana induced  apoptosis  on  T47D cell.Keywords: apoptosis, breast cancer, Selaginella
EFFECT OF BENZALDEHYDE EXCESS IN THE SYNTHESIS OF LR-2 AND CYTOTOXIC ACTIVITY OF LR-2 AGAINTS HeLa CELL Ritmaleni, Ritmaleni; Arifin, Muhammad Fajar; Laksmiani, Ni Putu Linda; ., Rumiyati; ., Sismindari
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.791 KB) | DOI: 10.14499/indonesianjpharm0iss0pp9-17

Abstract

LR-2(4-phenyl-3,4-dihydro-indeno[2’,1’]pyramidine-2(1H)- thione;  Leni  Ritmaleni  2),  which  designed  and  assumed  to  have biologically  activity  as  anticancer,  has  been  successfully synthesized  by  using  the  Biginelli  reaction.  This  research  was aimed  to  investigate  the  effect  of  benzaldehyde  excess  in  the synthesis  of  LR-2  and  to  evaluate  the  cytotoxic  activity  of  LR-2against HeLa cancer cell lines. The synthesis was done by reacting benzaldehyde, 2-indanone and together  with thiourea at one time as  said  as  one  pot  reaction  synthetic  methodology  and  the reaction was acid catalysed. The mole equivalent of benzaldehyde was  in  excess  compare  to  others.  The  effect  of  benzaldehyde  in excess is the higher the mole of benzaldehyde, the lower the yield of  LR-2.  The  cytotoxicity  of  LR-2  was  done  by  using  MTT  method and the LC50 was 268.15 μM.Key words : LR-2, benzaldehyde, cytotoxic, HeLa 
APPLICATION OF FOURIER TRANSFORM INFRARED SPECTROSCOPY FOR QUALITY CONTROL OF PHARMACEUTICAL PRODUCTS: A REVIEW Rahman, Abdul
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.646 KB) | DOI: 10.14499/indonesianjpharm0iss0pp1-8

Abstract

Analysis  of  pharmaceutical  products  covers  all  aspects  of quality  control  of  active  pharmaceutical  ingredients  (API)  and finished  products.  Today,  Fourier  transform  infrared  (FTIR)spectroscopy, especially in combination with chemometrics software, has  emerged  as  one  of  the romising  analytical  techniques  to  be used  in  pharmaceutical  industry,  for  quality  control  of  desired pharmaceutical  products.  Compared  with  other  instrumental techniques, FTIR spectroscopy offers  some advantages,  namely  it  is rapid,  simple  in  sample  preparation,  and  not  destructive.  In  this review,  the  application  of  FTIR  spectroscopy  for  qualitative  and quantitative  determinations  of  API  and  monitoring  drug  release  are described.Key words: FTIR spectroscopy, quality control, pharmaceutical products.
CHROMOSOME CHARACTERIZATION OF THREE VARIETIES OF GINGER (Zingiber officinaleRosc.) Daryono, Budi Setiadi; Rahma, Siti Nur Azizah Fauziati; ., Purnomo; ., Sudarsono
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.764 KB) | DOI: 10.14499/indonesianjpharm0iss0pp54-59

Abstract

Giant  ginger  (Zingiber  officinale Rosc.  var.  officinale),  red ginger  (Zingiber  officinale Rosc.  var.  rubra)  and  small  ginger (Zingiber  officinale Rosc.  var.  amarum)  are  three  varieties  of Zingiber officinale Rosc. They have a lot of benefit and often used by  Indonesian  as  a  traditional  drug.  Moreover,  they  have  a  big chance to be use as a flavor in world wide. Therefore, research for their  quality,  quantity  and  continuity  of  supplyare  needed. Characterization  of  their  chromosomes  is  one  effort for  improving ginger  cultivation.  The  objective  of  this  research  was  to  study mitotic  time  and  chromosome  characters  of  three  varieties  of ginger.  Squashing method was used for chromosome preparation. The  results  showed  that  mitotic  time  of  giant  ginger  is  09.00-10.05  am,  red  ginger  is  09.00-10.30  am,  while  small  ginger  is 08.45-11.00  am.  Chromosome  number  of  giant  ginger  and  small ginger are 2n=2x=30, while red ginger is 2n=2x=22. Giant ginger has R= 3,109, Red ginger has R = 3,206 and small ginger has R = 4,065.  Based  on  chromosome  characters  it  is  revealed  that relationship  between  giant  ginger  and  red  ginger  is  closer  that  of compare  to  small  ginger.  This  result  is  important  as  basic information for improving the gingers production through breeding program.Key  words:  Zingiber  officinale Rosc.,  mitotic  time,  chromosome characterization, squashmethod
PHYTOCHEMICAL SCREENING AND ANALYSIS POLYPHENOLIC ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF WHITE DRAGON FRUIT (Hylocereus undatus) VH, Elfi Susanti; Utomo, Suryadi Budi; Syukri, Yandi; Redjeki, Tri
INDONESIAN JOURNAL OF PHARMACY Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.544 KB) | DOI: 10.14499/indonesianjpharm0iss0pp60-64

Abstract

White dragon fruit  is  a well  known  and  widely  used  herbal medicine,  especially  in  Asia,  which  contains  several  interesting bioactive constituents and possesses health promoting properties. The  aim  of  this  study  was  to  analyze  for  the  bioactive compounds,  evaluate  total  phenolic  contents  and  antioxidant capacities  of  methanolic  extract  of  white  dragon  fruit.  The antioxidant  activity  was  determined  by  the  1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. Total phenolic  content  were  determined  by  Folin-Ciocalteu  method. Phytochemical  screening  of  the  white  dragon  fruit  showed the  presence  of  triterpenoid,  alkaloid,  flavonoid  and  saponin. The  extract  exhibited  strong  antioxidant  activity  with  IC50 of 193 μg/mL, and total phenolic content of 246 μg/mL in 1 Kg dry extract.Key words: antioxidant activity, total phenolic, DPPH, white dragon fruit

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