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BIO-SITE |BIOLOGI Sains Terapan
Published by Universitas Jambi
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34
Articles
EVALUATION OF Jatropha curcas L. SAP AS A SKIN ANTISEPTIC AGAINST Staphylococcus aureus, Escherichia coli, AND Candida albicans GROWTH

Harfiani, Erna

BIO-SITE |Biologi dan Sains Terapan Vol 4 No 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Skin disease is still a health problem in the world, especially tropical countries, including Indonesia, it needs normal control of flora among others with antiseptic. Jatropha curcas L., which contains secondary metabolite compounds suspected as an antiseptic on the skin. The study aimed to evaluate Jatropha sap can inhibit the growth of Staphylococcus aureus, Escherichia coli, and Candida albicans, and to know the optimal concentration of Jatropha curcas by invitro. The antiseptic activity of Jatropha sap was tested using agar diffusion method (Kirby-Bauer) with Mueller Hinton Agar medium and Saboround Dextrose Agar at concentrations 20%, 40%, 60%, 80% and 100. The results showed that Jatropha sap can inhibit the growth of Staphylococcus aureus, and Escherichia coli but not in Candida albicans. Treatment of 100% Jatropha sap concentration is the most optimal concentration in inhibiting the growth of Staphylococcus aureus, and Escherichia coli with an inhibitory zone of 9.75 ± 0.27 mm (medium antiseptic power) and 10.61 ± 0.2 mm (strong antiseptic power). The results show that the sap of Jatropha curcas L. grown in Indonesia has a good potential to be used as an antiseptic made from nature.   Penyakit pada kulit masih merupakan masalah kesehatan di dunia terutama negara beriklim tropis, termasuk Indonesia, sehingga diperlukan pengendalian flora normal diantaranya dengan antiseptik. Getah jarak pagar (Jatropha curcas L.) yang mengandung senyawa metabolit sekunder diduga berfungsi sebagai antiseptik pada kulit. Penelitian bertujuan mengevaluasi apakah getah jarak pagar dapat menghambat pertumbuhan Staphylococcus aureus, Escherichia coli dan Candida albicans, dan mengetahui konsentrasi optimal getah jarak pagar secara invitro. Aktivitas antiseptik getah jarak pagar diuji dengan menggunakan metode difusi agar (Kirby-Bauer) dengan medium Mueller Hinton Agar (MHA) dan Saboround Dextrose Agar (SDA) pada konsentrasi 20%, 40%, 60%, 80% dan 100. Hasil penelitian menunjukkan getah jarak pagar dapat menghambat pertumbuhan bakteri Staphylococcus aureus, dan Escherichia coli namun tidak pada jamur Candida albicans. Perlakuan getah jarak pagar konsentrasi 100% merupakan konsentrasi paling optimal dalam menghambat pertumbuhan Staphylococcus aureus, dan Escherichia coli dengan zona hambat 9,75 ± 0,27 mm (daya antiseptik sedang) dan 10,61  0,2 mm (daya antiseptik kuat). Hasil menunjukkan getah jarak pagar yang banyak tumbuh di Indonesia mempunyai potensi yang cukup baik untuk dipergunakan sebagai antiseptik berbahan alam.   Keyword:  Antiseptik, Escherichia coli, Candida albicans, Jarak pagar (Jatropha curcas L.),  Staphylococcus aureus  

Kemampuan Air Kelapa Muda Sebagai Antimikroba Terhadap Bakteri Escherichia coli Penyebab Diare

Mulyanto, Arif, Mujahid, Ikhsan, Khasanah, Tyas Ulfah

BIO-SITE |Biologi dan Sains Terapan Vol 4 No 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

  Diarrhea is a circumstance when the body loses much fluid and electrolyte through feces. According to the data obtained by Ministry of Health of the Republic of Indonesia (2016), in 2015 there were 18 cases of healthemergency of diarrhea, spread over 11 provinces and 18 regencies/cities. There were 1.213 people who got the disease, 30 of them died. Partially, villagers treated diarrhea by drinking young coconut water. This research aimsto discover the effectiveness of several kinds of young coconut water as antimicrobials to bacteria that can causediarrhea (bacteria test) such as Escherichia coli. In this research the inhibitionof young coconut water sample toward above-mentioned bacteria was held. Besides, a phytochemical screeningtest was also conducted to find out the antimicrobial substances contained in young coconut water. The data of young coconut water antimicrobial testing toward Escherichia coli, wereanalyzed by employing analysis of varian (ANOVA) with F test on 95% confidence level. Data testing wasfollowed by Duncan test on 95% confidence level. Research results indicated that young coconut water had antimicrobial ability toward Escherichia coli. Based on the result ofphytochemical screening of the young coconut water (all treatments) it was only secondary metabolite tannins detected. All treatments (of the young coconut water) could inhibit tested bacteria. It is allegedly because thewater contained tannins. The water of green coconut (used as a traditional medicine) was an effective inhibitor of bacteria growth bacteri. On E. coli the inhibition zone diameter was 11,6 mm. Keywords: young coconut water, antimicrobial, bacteria, diarrhea

ISOLASI DAN IDENTIFIKASI Shigella sp. PENYEBAB DIARE PADA BALITA

Aini, Fitratul

BIO-SITE |Biologi dan Sains Terapan Vol 4 No 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

     Diarrhea is the highest cause of death for toddlers. This study aims to isolate andidentify the bacteria Shigella sp. causes of diarrhea in toddlers. Diarrhea samples wereobtained from RSIA Annisa, Jambi City then isolation was carried out at the Biotechnologyand Engineering Laboratory of the Faculty of Science and Technology, Jambi Universityusing SSA media and dilution and biochemical tests were carried out. The results ofbacterial isolates were characterized and obtained by Salmonella sp. isolates. With thecharacteristic of black colonies, capable produce H2S gas and can ferment sugar. Keywords : Toddler, Diarrhea, Jambi, Salmonella sp. , Shigella sp.      

Keanekaragaman Kumbang Sungut Panjang (Cerambycidae) di Kawasan Hutan Pendidikan Universitas Jambi

Wulandari, Tia, Kartika, Winda Dwi

BIO-SITE |Biologi dan Sains Terapan Vol 4 No 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Abstrak Serangga adalah kelompok hewan yang memiliki keragaman lebih tinggi daripada yang lain. Serangga memiliki manfaat dalam ekosistem dan juga dapat membantu aktivitas manusia. Cerambycid adalah sejenis serangga yang dapat mengubah komponen organik pada kayu dan batang mati. Tujuan dari penelitian ini adalah untuk mengetahui keragaman Cerambycid di hutan pendidikan Universitas Jambi. Penelitian ini diadakan pada bulan Agustus sampai November 2016. Sampel dikumpulkan dengan menggunakan perangkap Arthocarpus di 3 tempat yang berbeda. Jumlah keramik yang telah dikumpulkan adalah 561 individu dan termasuk 14 spesies. Hasil analisis data menunjukkan tingkat keragaman 0,631. Nilai indeks keanekaragaman dapat menunjukkan kondisi hutan pendidikan Universitas Jambi yang baik sebagai habitat kumbang Cerambycid.   Kata Kunci: kumbang, Cerambycid, keragaman, kumbang sungut panjang   Abstract   Insects are group of animals that have higher diversity than anothers. Insects have benefits in ecosystem and also can help human activities. Cerambycid is a kind of insects that can transform organic matters on wood and dead stem. The aim of this study is to know the diversity of Cerambcyid in Jambi University educational forest. It is held on August untill November 2016. Samples was collected by using Arthocarpus trap in 3 different spot. The number of Cerambycid that had collected are 561 individuals and belong to 14 species. The result of data analysis showed that Cerambcyid diversity rate is classified into medium rate with diversity value reaches 0,631. Diversity index value can show Jambi University educational forest condition which is good as the Cerambcyid beetle.   Keyword: Beetle, Cerambycid, Diversity, Long Horn Beetle

Deteksi Cemaran Bakteri Escherichia coli dalam Jaruk Tigaron pada Pasar Sungai Andai dan Pasar Lama Kota Banjarmasin

Susilawati, Ika Oksi, Saidah, Rini

BIO-SITE |Biologi dan Sains Terapan Vol 4 No 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Jaruk tigaron is one of the traditional fermented foods originating from South Kalimantan made by fermented tigaron flowers (Crataeva nurvala, Buch HAM). The process of making traditionally allows contamination by the bacteria Eschericia coli (E.coli). Escherichia coli is the most commonly used a microbe as an indicator of faecal contamination in water, even food or beverages, including various types of jaruk. This final project aims to detect Escherichia coli on jaruk tigaron thatdistributed in Banjarmasin. The method was used in detecting Escherichia coli is the Most Probable Number (MPN) with five test stages are estimation test, confirmatory test, complementary test, biochemical test (TSIA and IMVIC). The media was used is a Lactose Broth Single Strength (LBSS) by 3 series for forecasting test, Brilliant Green Bile Broth (BGLB) media for confirmatory test, Eosin Methylene Blue Agar (EMBA) media for complementary test, TSIA test and IMVIC test. The test results was obtained by MPN index on samples (a) 28 samples (b)> 1.100 and sample (c) 20. The detection results is Escherichia coli on jaruk tigaron thatdistributed in Banjarmasin declared negatively containing Escherichia coli but only contain Coliform bacteria. Based on SNI 7388: 2009, the determination of the maximum limit of microbial contamination in food is the absence of Coliform and Escherichia coli bacteria in 100 ml of sample.

Pengaruh Pemberian Bokashi Campuran Alang-alang (Imperata cylindrica L.) dan Kotoran Kambing Terhadap Pertumbuhan Tanaman Sawi (Brassica rapa L.)

Susilo, Susilo, Triansyah, Liza Verizza, Setyaningsih, Maryanti

BIO-SITE |Biologi dan Sains Terapan Vol 4 No 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing terhadap pertumbuhan tanaman sawi (Brassica rapa L.). Metode penelitian yang digunakan adalah metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL), yang terdiri dari 5 perlakuan dengan 5 ulangan. Parameter pertumbuhan yang diamati dalam penelitian ini meliputi berat segar, panjang akar dan berat kering tanaman. Hasil penelitian menunjukkan bahwa perlakuan P2 (dosis 20 g) mendapatkan nilai rata-rata terbaik untuk berat segar dan berat kering yaitu secara berturut-turut 30,692 g dan 1,680 g, dengan nilai persentase yaitu 301,49% dan 390,70% terhadap kontrol. Sedangkan pada parameter panjang akar tanaman hasil nilai rata-rata paling baik terdapat pada P3 yaitu 5,46 cm dengan persentase 177,27% terhadap kontrol. Berdasarkan uji statistik yang dihitung menggunakan uji ANAVA 1 Faktor, pertumbuhan tanaman sawi pada parameter berat segar, panjang akar dan berat kering tanaman yang secara berturut-turut menunjukkan bahwa Fhitung ≥ Ftabel, yaitu 3,40 > 2,87; 2,87 ≥ 2,87 dan 4,86 > 4,43. Oleh karena Fhitung ≥ Ftabel, maka kelima perlakuan dinyatakan berpengaruh signifikan terhadap parameter berat segar dan panjang akar tanaman dan berpengaruh sangat signifikan pada parameter berat kering tanaman. Berdasarkan hasil tersebut dapat disimpulkan bahwa bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing berpengaruh terhadap pertumbuhan tanaman sawi (Brassica rapa L.).

Uji Aktivitas Antibakteri Ekstrak Etanol Biji Ganitri (Elaeocarpus sphaericus Schum.) terhadap Pertumbuhan Bakteri Penyebab Demam Typhoid secara In vitro”.

Maesyaroh, Dewi, Imansyah, Ega Bramaseta, Nafratilova, Hilda Febiana, Anggarani, Nimas Putri, Sunawan, Sunawan

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

 Abstrak  Penelitian yang dilakukan di  Laboratorium Biologi Universitas PGRI Banyuwangi ini, bertujuan untuk mengetahui aktivitas antibakteri  ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) dalam menghambat pertumbuhan bakteri penyebab demam typhoid secara in vitro dan untuk mengetahui Konsentrasi Hambat Minimum (KHM) ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.). Metode yang digunakan ialah metode sumuran dengan control positif tetrasiklin 1% dan control negative aquades steril. Serial konsentrasi ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) yang digunakan sebesar 10%, 20%, 30%, 40%, dan 50%, dan pada uji KHM menggunakan serial konsentrasi sebesar 1%, 2,5%, 5%, 7,5%, dan 10%. Berdasarkan hasil uji, zona hambat terbesar ditunjukkan oleh konsentrasi 50% dengan rata-rata diameter zona hambat sebesar 1.030 cm dan zona hambat terkecil ditunjukkan konsentrasi 10% dengan rata-rata diameter zona hambat sebesar 0.203 cm. Hasil uji ANOVA nilai (F.Hit>F. Tabel) dengan F.Hit sebesar 17.638 dan F.Tabel sebesar 2.85 serta nilai signifikasi sebesar 0.000 (P<0.05), karena nilai P<0.05 maka dapat disimpulkan bahwa terdapat Pengaruh konsentrasi ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) terhadap pertumbuhan bakteri uji. Hasil uji Duncan menunjukkan ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.), pada konsentrasi 50% mempunyai zona hambat yang berbeda nyata atau berbeda signifikan terhadap konsentrasi perlakuan 10%, 20%, 30%, kontrol negatif dan kontrol positif, namun tidak berbeda nyata dengan konsentrasi 40%. Sedangkan KHM ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) yang masih mampu menghambat pertumbuhan bakteri uji pada konsentrasi 1% dengan rata-rata diameter zona hambat sebesar 0.037 cm.

Isolasi dan Identifikasi Cemaran Bakteri Salmonella sp. pada Daging Ayam dan Ikan Mentah

Maritsa, Hasna Ul, Aini, Fitratul, saputra, Ardiansyah, nurhakim, Desri santi, sihombing, Greace meisinta

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Food is a basic need that must be free from microorganisms contamination Salmonella sp. is a gram-negative bacteria that often contaminates food, especially meat, and causes Salmonellosis disease. This study aims to isolate and identify Salmonella sp. which allegedly contaminating the sample of chicken meat and raw fish. Samples from traditional market around Jambi University. The tests were conducted at the Laboratory of Biotechnology and Engineering, Faculty of Science and Technology, Jambi University. Isolation was performed by dilution method on SSA medium (Salmonella Shigella Agar), isolates were characterized by macroscopic-microscopic morphology, and biochemical test. The results showed that raw chicken meat was positively contaminated with Salmonella sp., while fish meat was not. Gram-negative bacillus isolate character is yields H2S black deposits and is capable of fermenting glucose, lactose and sucrose.

Kajian Tanaman Penyerap Timbal (Pb) dan Pengikat Karbon di Lingkungan Kampus Universitas Jambi

Aini, Fitratul, Mardiyah, Siti, Wahyuni, Fitri, Millah, Aulia Ul, Ihsan, Mahya

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Kampus Universitas Jambi di daerah Mendalo memiliki jumlah mahasiswa terbanyak dibandingkan dengan kampus UNJA yang lain, dan sebagian besar mahasiswa menggunakan kendaraan bermotor. Kampus ini juga berada di jalan lintas sumatera yang dilalui oleh banyak kendaraan yang menghasilkan asap yang mempengaruhi polusi udara di sekitar kampus. Berbagai jenis tanaman diyakini dapat menyerap karbon dan timbal (Pb) sehingga mampu mengurangi dampak polusi. Untuk itu perlu dilakukan penelitian untuk mengetahui tanaman yang efektif menyerapan timbal (Pb) dan mengikat karbon pada beberapa tanaman di Kampus Universitas Jambi, Mendalo. Penelitian ini dilaksanakan selama 6 bulan di Laboratorium Bioteknologi dan Rekayasa, FST UNJA dan Laboratorium Terpadu UNPAD Bandung. Metode dalam penelitian ini menggunakan metode survei untuk menentukan lokasi penelitian yaitu gerbang utama (stasiun I), gerbang ATM (Stasiun II), area rektorat (Stasiun III), area fakultas ekonomi (Stasiun IV), dan area FST serta kehutanan (Stasiun V). Data yang dikumpulkan berupa kadar timbal (Pb) dan karbon. Hasil penelitian menunjukkan bahwa tanaman yang mampu menyerap kadar timbal terbesar adalah  bintaro sedangkan tanaman penyerap karbon terbaik adalah mahoni. Besarnya kadar timbal dan karbon tanaman dipengaruhi beberapa faktor diantaranya lokasi pengambilan sampel, intensitas kendaraan, umur daun tanaman, dan jenis tanaman

Biologi Reproduksi dan Polinasi Buatan Tumbuhan Senduduk (Melastoma malabathricum L.)

Suryani, Lilis

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Abstract Melastoma malabthricum is the type with the widest area of spread compared with other species in the genus Melastoma. This research uses floral phenology observation method and pollination experiment by using bagging technique, emasculation and artificial pollination. Stigma receptivity was tested according to the Matson method. The results showed that M.  malabathricum’s flowers take 14 until 21 days from bud to flower bloom. Stigma M.  malabathricum is already present and receptive before anthesis flower. Fruit can be formed through self pollination (autogamy) and cross pollination (xenogamy). M.  malabathricum pollination approaches the xenogamy facultative.