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BIO-SITE |BIOLOGI Sains Terapan
Published by Universitas Jambi
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29
Articles
Pengaruh Pemberian Bokashi Campuran Alang-alang (Imperata cylindrica L.) dan Kotoran Kambing Terhadap Pertumbuhan Tanaman Sawi (Brassica rapa L.)

Susilo, Susilo, Triansyah, Liza Verizza, Setyaningsih, Maryanti

BIO-SITE |Biologi dan Sains Terapan Vol 4 No 1 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing terhadap pertumbuhan tanaman sawi (Brassica rapa L.). Metode penelitian yang digunakan adalah metode eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL), yang terdiri dari 5 perlakuan dengan 5 ulangan. Parameter pertumbuhan yang diamati dalam penelitian ini meliputi berat segar, panjang akar dan berat kering tanaman. Hasil penelitian menunjukkan bahwa perlakuan P2 (dosis 20 g) mendapatkan nilai rata-rata terbaik untuk berat segar dan berat kering yaitu secara berturut-turut 30,692 g dan 1,680 g, dengan nilai persentase yaitu 301,49% dan 390,70% terhadap kontrol. Sedangkan pada parameter panjang akar tanaman hasil nilai rata-rata paling baik terdapat pada P3 yaitu 5,46 cm dengan persentase 177,27% terhadap kontrol. Berdasarkan uji statistik yang dihitung menggunakan uji ANAVA 1 Faktor, pertumbuhan tanaman sawi pada parameter berat segar, panjang akar dan berat kering tanaman yang secara berturut-turut menunjukkan bahwa Fhitung ≥ Ftabel, yaitu 3,40 > 2,87; 2,87 ≥ 2,87 dan 4,86 > 4,43. Oleh karena Fhitung ≥ Ftabel, maka kelima perlakuan dinyatakan berpengaruh signifikan terhadap parameter berat segar dan panjang akar tanaman dan berpengaruh sangat signifikan pada parameter berat kering tanaman. Berdasarkan hasil tersebut dapat disimpulkan bahwa bokashi campuran alang-alang (Imperata cylindrica L.) dan kotoran kambing berpengaruh terhadap pertumbuhan tanaman sawi (Brassica rapa L.).

Uji Aktivitas Antibakteri Ekstrak Etanol Biji Ganitri (Elaeocarpus sphaericus Schum.) terhadap Pertumbuhan Bakteri Penyebab Demam Typhoid secara In vitro”.

Maesyaroh, Dewi, Imansyah, Ega Bramaseta, Nafratilova, Hilda Febiana, Anggarani, Nimas Putri, Sunawan, Sunawan

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

 Abstrak  Penelitian yang dilakukan di  Laboratorium Biologi Universitas PGRI Banyuwangi ini, bertujuan untuk mengetahui aktivitas antibakteri  ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) dalam menghambat pertumbuhan bakteri penyebab demam typhoid secara in vitro dan untuk mengetahui Konsentrasi Hambat Minimum (KHM) ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.). Metode yang digunakan ialah metode sumuran dengan control positif tetrasiklin 1% dan control negative aquades steril. Serial konsentrasi ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) yang digunakan sebesar 10%, 20%, 30%, 40%, dan 50%, dan pada uji KHM menggunakan serial konsentrasi sebesar 1%, 2,5%, 5%, 7,5%, dan 10%. Berdasarkan hasil uji, zona hambat terbesar ditunjukkan oleh konsentrasi 50% dengan rata-rata diameter zona hambat sebesar 1.030 cm dan zona hambat terkecil ditunjukkan konsentrasi 10% dengan rata-rata diameter zona hambat sebesar 0.203 cm. Hasil uji ANOVA nilai (F.Hit>F. Tabel) dengan F.Hit sebesar 17.638 dan F.Tabel sebesar 2.85 serta nilai signifikasi sebesar 0.000 (P<0.05), karena nilai P<0.05 maka dapat disimpulkan bahwa terdapat Pengaruh konsentrasi ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) terhadap pertumbuhan bakteri uji. Hasil uji Duncan menunjukkan ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.), pada konsentrasi 50% mempunyai zona hambat yang berbeda nyata atau berbeda signifikan terhadap konsentrasi perlakuan 10%, 20%, 30%, kontrol negatif dan kontrol positif, namun tidak berbeda nyata dengan konsentrasi 40%. Sedangkan KHM ekstrak etanol biji ganitri (Elaeocarpus sphaericus Schum.) yang masih mampu menghambat pertumbuhan bakteri uji pada konsentrasi 1% dengan rata-rata diameter zona hambat sebesar 0.037 cm.

Isolasi dan Identifikasi Cemaran Bakteri Salmonella sp. pada Daging Ayam dan Ikan Mentah

Maritsa, Hasna Ul, Aini, Fitratul, saputra, Ardiansyah, nurhakim, Desri santi, sihombing, Greace meisinta

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Food is a basic need that must be free from microorganisms contamination Salmonella sp. is a gram-negative bacteria that often contaminates food, especially meat, and causes Salmonellosis disease. This study aims to isolate and identify Salmonella sp. which allegedly contaminating the sample of chicken meat and raw fish. Samples from traditional market around Jambi University. The tests were conducted at the Laboratory of Biotechnology and Engineering, Faculty of Science and Technology, Jambi University. Isolation was performed by dilution method on SSA medium (Salmonella Shigella Agar), isolates were characterized by macroscopic-microscopic morphology, and biochemical test. The results showed that raw chicken meat was positively contaminated with Salmonella sp., while fish meat was not. Gram-negative bacillus isolate character is yields H2S black deposits and is capable of fermenting glucose, lactose and sucrose.

Kajian Tanaman Penyerap Timbal (Pb) dan Pengikat Karbon di Lingkungan Kampus Universitas Jambi

Aini, Fitratul, Mardiyah, Siti, Wahyuni, Fitri, Millah, Aulia Ul, Ihsan, Mahya

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Kampus Universitas Jambi di daerah Mendalo memiliki jumlah mahasiswa terbanyak dibandingkan dengan kampus UNJA yang lain, dan sebagian besar mahasiswa menggunakan kendaraan bermotor. Kampus ini juga berada di jalan lintas sumatera yang dilalui oleh banyak kendaraan yang menghasilkan asap yang mempengaruhi polusi udara di sekitar kampus. Berbagai jenis tanaman diyakini dapat menyerap karbon dan timbal (Pb) sehingga mampu mengurangi dampak polusi. Untuk itu perlu dilakukan penelitian untuk mengetahui tanaman yang efektif menyerapan timbal (Pb) dan mengikat karbon pada beberapa tanaman di Kampus Universitas Jambi, Mendalo. Penelitian ini dilaksanakan selama 6 bulan di Laboratorium Bioteknologi dan Rekayasa, FST UNJA dan Laboratorium Terpadu UNPAD Bandung. Metode dalam penelitian ini menggunakan metode survei untuk menentukan lokasi penelitian yaitu gerbang utama (stasiun I), gerbang ATM (Stasiun II), area rektorat (Stasiun III), area fakultas ekonomi (Stasiun IV), dan area FST serta kehutanan (Stasiun V). Data yang dikumpulkan berupa kadar timbal (Pb) dan karbon. Hasil penelitian menunjukkan bahwa tanaman yang mampu menyerap kadar timbal terbesar adalah  bintaro sedangkan tanaman penyerap karbon terbaik adalah mahoni. Besarnya kadar timbal dan karbon tanaman dipengaruhi beberapa faktor diantaranya lokasi pengambilan sampel, intensitas kendaraan, umur daun tanaman, dan jenis tanaman

Biologi Reproduksi dan Polinasi Buatan Tumbuhan Senduduk (Melastoma malabathricum L.)

Suryani, Lilis

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 2 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract Melastoma malabthricum is the type with the widest area of spread compared with other species in the genus Melastoma. This research uses floral phenology observation method and pollination experiment by using bagging technique, emasculation and artificial pollination. Stigma receptivity was tested according to the Matson method. The results showed that M.  malabathricum’s flowers take 14 until 21 days from bud to flower bloom. Stigma M.  malabathricum is already present and receptive before anthesis flower. Fruit can be formed through self pollination (autogamy) and cross pollination (xenogamy). M.  malabathricum pollination approaches the xenogamy facultative.

Pertumbuhan Tanaman Sawi Hijau (Brassica juncea L.) dengan Pemberian Kompos Berbahan Dasar Daun Paitan (Thitonia diversifolia)

Istarofah, Istarofah, Salamah, Zuchrotus

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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This study aimed to determine the growth of mustard green (Brassica juncea L.) by addion Paitan leaves (Tithonia diversifolia) based compost at different doses and to determine the optimum dose of compost. This study used a completely randomized design (CRD) with one factor that is the dose of Paitan leaves based compost and the treatments were: A1 = 0.25 kg of compost, A2 = 0.5 kg of compost, A3 = 0.75 kg of compost, A4 = 1 kg of compost, A5 = 1.25 kg of compost and control (without compost), and each treatment was added by the soil so that the total weight was 2.5 kg in each polybag. Observation parameters were the plant height, leaf number, leaf length, leaf width, wet weight and dry weight of plants.  The data were tested with Analysis of Variant (ANOVA) and followed by LSD test at 5% level. Based on the results of this study, it was concluded that the growth of mustard green plant by addition Paitan leaves based compost at various doses showed different growth of plants. the A3 Treatment is the most optimum dose to raise the growth of mustard green.  

Isolation of Fungal Endophytes from Melastoma malabathricum L. and Their Potential as Antifungal

Octavianti, Rusi, harahap, Israwati, Elsie, Elsie

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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The aim of this study was to isolation the endophytic fungi from medicinal plant Melastoma malabathricum L. and to investigate their potential antifungal activity. Isolation endophytic fungi was carried out using surface sterilized method. The fungal extracts were assessed for antifungal activity against Candida albicans using agar well diffusion method. A total of 28 isolates fungal endophytes from leaves, stems, flowers and roots of Melastoma malabathricum L. The results showed that of twelev have strong category within inhibit growth of C. albicans. Isolate 18 is endophytic fungi who produced the largest zones of inhibit is 19 mm.

Formulasi Media Produksi Bibit F2 Jamur Tiram Putih

Susilawati, Ika Oksi, Imaningsih, Witiyasti, Mulyanto, Arif

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

White oyster mushroom (Pleurotus ostreatus L.) is a fungus that consumption demand by people Banjarbaru. However, the fulfillment of this fungus is still lacking, has always supplied from Java. Based on these problems, it is necessary to study the composition of the growth media F2 seeds oyster mushroom. The purpose of this study was to determine the composition ratio of constituents of seedling growth media appropriate for the growth of white oyster mushroom seeds. This research was conducted by dividing some compositions media. Comparison of composition constituents of the right media is used to make seedling growth media F2 is a mixture of sawdust with grain (50%: 50%). In addition the use of pure sawdust can also be used as a media for the manufacture of white oyster mushroom F2 seeds. The composition of the media mix sawdust with grain (50%: 50%) effectively used to create F2 seed oyster mushroom.

Keragaman Lumut Epifit di Hutan Kota dan Tepi Jalan Utama Kampus Universitas Indonesia

Putrika, Afiatri, Nisyawati, Nisyawati, Ariyanti, Nunik Sri

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Research on epiphytic bryophytes has been conducted in two different sites located in Universitas Indonesia (UI).  Those sites were urban forest and vegetation on main street margin of the campus.  This study was carried out to compare diversity of the bryophyte at both sites.  Twelve plots of 25 x 25 m2 were establish at the forest, while nine of 50 m line transect were made at the street margin.  Five trees of each plot or line transect were sampled.  Eight sub plots of 15 x 15 cm2 were placed on each trunk base (0--200 cm) of the tree sampels.  The results obtained 23 species of epiphytic bryophytes, 21 species occured in the forest and 14 species were found at street margin.  The similarity of bryophyte community between the forest and street margin were high (Sorenson similarity index = 0.73).  Octoblepharum albidum was the dominant species at the forest, while Calymperes tenerum was dominant at the street margin.  The diversity of epiphyte bryophyte at both sites were categorized low based on Shannon Wiener index (H’< 2), however they were not different significantly.

Efektivitas Bakteri Amilolitik Asal Geopark Merangin Jambi terhadap Patogenitas Jamur Pyricularia oryzae Penyebab Penyakit Blas Daun Padi

Batubara, Ummi Mardhiah, Suparjo, Suparjo, Maritsa, Hasnaul, Tari, Nanda Fahmuin, Andriani, Selvi

BIO-SITE |Biologi dan Sains Terapan Vol 3 No 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Rice (Oryza sativa) is a major crop product in Asia. Rice products are increasing along with the increasing of human population. Blast disease in leaf (leaf blast) is the major pathogen in rice during vegetative phase. The using of amylolytic bacteria isolated from Geopark Merangin Jambi is done as an attempt to discover biocontrol agents in order to attack infection of Pyricularia oryzae, the cause of leaf blast disease. Antimicrobial activity assay was carried out by isolating the pathogen in the infected rice crops. Pyricularia oryzae that had been cultured then were evaluated their antagonistic activity against amylolytic bacteria isolated from prior experiment. Results have shown that from 25 isolates of amylolytic bacteria taken from Geopark Merangin Jambi, 2 isolates that can be used as biocontrol agents were GM20 and GM23 capable of inhibiting the growth of Pyricularia oryzae.