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BIO-SITE |BIOLOGI Sains Terapan
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Articles 40 Documents
SELEKTIVITAS ANTIKANKER EKSTRAK DAUN SIRSAK (Annona muricata) PADA LINI SEL KANKER PAYUDARA Fatmawati, Dina; Suparmi, Suparmi; Yusuf, Iwang; Isradji, Israhnanto
BIO-SITE |Biologi dan Sains Terapan Vol 4 No 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.984 KB) | DOI: 10.22437/bs.v4i2.5440

Abstract

Studies have been conducted on cytotoxicity of sirsak (Annona muricata) against T47D cell line. However, the selectivity of Annona muricata leaf extract against MCF-7 breast cancer has not established. This study aimed to determine the index selectivity of Annona muricata leaf extract against MCF-7 breast cancer cell line. In this quasy experimental study using 2 kind of cell line, The MCF-7 breast cancer cell line and Vero cell line as normal cell were treated with Annona muricata leaf extract at dose 1000, 500, 250, 125, 62.5, 31.5 µg/mL respectively. The MTT assay were used to evaluate the viability of both cell line after treatment with Annona muricata leaf extract.  The data of viability cells were analyzed with Probit regression to determine the IC50 value of Annona muricata leaf extract in both cell lines. The selectivity of two treatment was determine based on the selectivity index (SI) that represents the ratio of IC50 value of Annona muricata leaf extract. The IC50 value of MCF-7 and normal cell were 44.94 and 184.04 respectively with selectivity index 4. This result suggested that Annona muricata had cytotoxic effect in MCF-7 breast cancer cell line but not in normal cells. In conclusion Annona muricata leaf extract has cytotoxic effect to MCF7 breast cancer cell selectively.
KOLONISASI BAKTERI ENDOFIT PADA AKAR TUMBUHAN ANDALEH (Morus macroura Miq.) Yandila, Sisri; Putri, Dwi Hilda; Fifendy, Mades
BIO-SITE |Biologi dan Sains Terapan Vol 4 No 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.117 KB) | DOI: 10.22437/bs.v4i2.5255

Abstract

Endophytic bacteria are bacteria living in plant tissues and symbiotic mutualism with their host. The excess production of bioactive substances from endophytic microorganisms among others, microorganisms are easy to grow, have shorter life cycles than plants and can produce large amounts of bioactive compounds. Endophytic bacteria found in plants generally originate from the roots and then spread through xylem tissue. Endophytic bacteria that live within the plant tissue for a certain period and are able to live by forming colonies in plant tissue without harming the host. Endophytic bacteria can be isolated from sterile plant tissue surfaces or extracted from inner plant tissue. The purpose of this study was to determine the type of bacterial isolate of edofit found in the root of Andaleh plant. The method used to isolate endophytic  bacteria from the root plant Andaleh  is streak plate method. Identification of bacteria macroscopically and microscopically. The results obtained were 16 isolates of endophytic bacteria from the roots of Andaleh plant seen from different colony forms. Identify microscopically with Gram staining
AKTIVITAS PELARUTAN FOSFAT ISOLAT Azospirillum spp. ASAL LAHAN PASIR BESI Wuryanto, Sodik; Oedjijono, Oedjijono
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
Publisher : BIO-SITE |BIOLOGI Sains Terapan

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Abstract

Iron sand lands have a low organic matter content as it contains of Iron (Fe) found nearly all over the coastal or beach in Indonesia. Phosphorous is one of macronutrient with considered role for plant promotion. One group of bacteria that are in an environment of rooting plants on land sand iron was Azospirillum spp. The purpose of this study was is to know the phosphate solubilizing activities of Azospirillum spp. isolates from land sand iron. The results showed that some isolates of Azospirillum spp. origin of iron sand land capable of solubilizing phosphate in qualitative and quantitative.   Key words: Azospirillum spp., phosphate, solubilization, iron sand
ANALISIS VEGETASI DI HABITAT ORANGUTAN, STASIUN PENELITIAN KETAMBE, TAMAN NASIONAL GUNUNG LEUSER, ACEH TENGGARA Nayasilana, Ike N; Atmoko, S.S Utami; Andayani, N
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
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Abstract

Orangutans occur in both primary and ex-logged forests of Ketambe Research station, Gunung Leuser National Park, Aceh. Vegetation analysis was carried out to compare the two habitat types, as a basis to understand the ecology of orangutans in disturbed habitats.  Data on 20 plots vegetation analysis 25x25 m (Juli 2010). The study recorded 275 trees from 99 species in the primary forest (67 species are food fruit trees), while the ex-logged forest contains 303 trees from 87 species (56 species are food fruit trees). Although the ex-logged forest still has pioneer species, such as Elateriospermum tapos and Macaranga sp., the two habitat types are not too dissimilar as shown by a relatively high index similarity (ISs = 59,70% for tree and 61% for liana). Diversity index (H’) of primary forest is also only slightly higher than of ex-logged forest (3,074 versus 2,961). These results indicate that natural succession during the last 8 years has been going relatively well. Key words: vegetation analysis, habitat type, natural succession
ISOLASI DAN IDENTIFIKASI BAKTERI TANAH YANG TERDAPAT DI SEKITAR PERAKARAN TANAMAN Sari, Dwi Ratna
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
Publisher : BIO-SITE |BIOLOGI Sains Terapan

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Abstract

The soil is one habitat of various bacteria. Rhizosphere microbes in the soil near the roots can be beneficial to the plants by forming nodules in some plants Leguminoceae. The bacteria are very helpful in chemical changes in the soil so that the chemicals can be absorbed by plants. The purpose of this research was to determine the type of bacteria that has been found in the soil near and away from plant roots. Bacteria was isolated from soil near and away from the roots at the level 10-5, 10-6, and 10-7 of dilution, each inoculated in duplicate on media Nutient Agar (NA) and incubated for 2x24 hours. Grown colonies and colonies that had thin, beige or transparent, size medium (3mm), and Gram negative characters were sub-cultured. Selected cultures were suspended in concentration about 108 cells / mL 30-50 mL. The identification used the API 20NE. One strip API 20NE. Identification results indicated a positive color change in the media. The research obtained bacteria that has been found near plant roots was Pseudomonas luteola, whereas the bacteria that has been found in away plant roots soil was P. aureuginosa.   Keyword: soil bacteria, plant roots
PERBANDINGAN KADAR ETANOL HASIL FERMENTASI SUBSTRAT DAMI NANGKA (Artocarpus heteropyllus Lamk.) DENGAN MENGGUNAKAN KULTUR MURNI Trichoderma viride DAN Saccharomyces cerevisiae Mardiyah, Siti; Widyaningrum, Trianik
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
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Abstract

Jackfruit’s dami (Artocarpus heterophyllus Lamk.) is a material that can be used as substrates in the manufacture of ethanol. The process of making ethanol fermentation is done by using a pure culture of Trichoderma viride and Saccharomyces cerevisiae. This study aims to determine the effect of treatment of a pure culture fermentation of the fungi Trichoderma viride on jackfruit’s dami (Artocarpus heterophyllus Lamk.) Against ethanol and compare the levels of ethanol fermented jackfruit’s dami substrate using Trichoderma viride pure culture and pure cultures of Saccharomyces cerevisiae. This study uses a completely randomized design (CRD) with factorial pattern using two factors: 1) Trichoderma viride and Saccharomyces cerevisiae, 2) Saccharomyces cerevisiae without Trichoderma viride. Furthermore, the data were analyzed using analysis of variance (ANOVA) and test T. If there is a significant difference, then continued with LSD test level of 5%. The results showed that higher levels of ethanol contained in the treatment without the use of Trichoderma viride and using Saccharomyces cerevisiae with a dose of 108 Cfu/ml which is 0.7851%, while the lowest levels of ethanol produced in the treatment with Trichoderma viride using doses of 5 ml and a dose of Saccharomyces cerevisiae 108 Cfu/ml is 0.1906 %.   Keywords : Fermentation, jackfruit’s dami, Trichoderma viride, Saccharomyces cerevisiae, Concentration of ethanol.
Sitotoksisitas Ekstrak Aspergillus fumigatus dari Daun Mekai (Albertisia papuana Becc.) terhadap Sel Kanker Payudara T47D dan MCF-7 Maritsa, Hasnaul; Moeljopawiro, Soekarti; Kasiamdari, Rina Sri
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
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Abstract

The previous studies showed that the Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root toxicity on breast cancer could not only by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them are leaves. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained of Tropical Medicine’s Faculty, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2 % medium. Secondary metabolism was extracted from fermented broth using in ethyl acetat and n-butanol. The cytotoxicity was perform by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus assosiated with A. papuana leaves. Ethyl acetat extract from fermented A. fumigatus both on T47D and MCF-7 cell lines had lower (IC50. 50, 444 µg/ml and 59 µg/ml) than n-butanol (IC50. 103, 398 µg/ml and 127,188 µg/ml. It could be said that A. fumigatus from A. papuana leaves could induce cytotoxicity on T47D and MCF-7 breast cancer cells.   Keywords: cancer, Aspergillus fumigatus, secondary metabolism, Albertisia papuana Becc., cytotoxiciy
VARIASI KARAKTER KUANTITATIF KEPITING Uca annulipes (BRACHYURA: OCYPODIDAE) di KEPULAUAN INDONESIA Murniati, Dewi Citra
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
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Abstract

Uca annulipes are deposit feeder crab which is widely distributed in Indonesian mangrove areas. The systematic study of the species completely based on the qualitative character without the quantitative one. This study describes the variation in quantitative characters of U. annulipes from different locations in Indonesia. Descriptive method with univariate and multivariate analyses based on quantitative character ratio was used in this study. The univariate analysis showed that the highest value of either the quantitative character or quantitative character ratio was found from the western Indonesian population.  Meanwhile, the multivariate analysis showed that U. annulipes from western Indonesia, Sulawesi, and Nusa Tenggara have a discrete cluster.
Aktivitas Pelarutan Fosfat Isolat Azospirillum spp. asal Lahan Pasir Besi Wuryanto, Sodik; Oedjijono, Oedjijono; Antonius, Sarjiya
BIO-SITE |Biologi dan Sains Terapan Vol 1 No 1 (2015): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.205 KB)

Abstract

Iron sand lands have a low organic matter content as it contains of Iron (Fe) found nearly all over the coastal or beach in Indonesia. Phosphorous is one of macronutrient with considered role for plant promotion. One group of bacteria that are in an environment of rooting plants on land sand iron was Azospirillum spp. The purpose of this study was is to know the phosphate solubilizing activities of Azospirillum spp. isolates from land sand iron. The results showed that some isolates of Azospirillum spp. origin of iron sand land capable of solubilizing phosphate in qualitative and quantitative.
Analisis Vegetasi di Habitat Orangutan, Stasiun Penelitian Ketambe, Taman Nasional Gunung Leuser, Aceh Tenggara Nayasilana, Ike; Atmoko, Utami; Andayani, N.
BIO-SITE |Biologi dan Sains Terapan Vol 1 No 1 (2015): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.301 KB)

Abstract

Orangutans occur in both primary and ex-logged forests of Ketambe Research station, Gunung Leuser National Park, Aceh. Vegetation analysis was carried out to compare the two habitat types, as a basis to understand the ecology of orangutans in disturbed habitats. Data on 20 plots vegetation analysis 25x25 m (Juli 2010). The study recorded 275 trees from 99 species in the primary forest (67 species are food fruit trees), while the ex-logged forest contains 303 trees from 87 species (56 species are food fruit trees). Although the ex-logged forest still has pioneer species, such as Elateriospermum tapos and Macaranga sp., the two habitat types are not too dissimilar as shown by a relatively high index similarity (ISs = 59,70% for tree and 61% for liana). Diversity index (H’) of primary forest is also only slightly higher than of ex-logged forest (3,074 versus 2,961). These results indicate that natural succession during the last 8 years hasbeen going relatively well.

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