Majalah Kedokteran Gigi Indonesia
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The effect of silk concentration on the flexural strength of FRC as a Bone Graft Moechtar, Rifki; Sunarintyas, Siti; Herliansyah, Muhammad Kusumawan
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.31487

Abstract

Bone fractures are mostly caused by trauma and disease. In the therapeutic process of bone healing which often meets some constraints, bone graft is mainly used to ensure that the healing process takes place. A fiber reinforced composite (FRC) is a popular bone graft material that is made to resemble bone properties. FRC is normally comprised of polymer matrix, hydroxyapatite filler, and fiber. Hydroxyapatite is a bioactive material widely used as a bone graft. Silk fiber is known as a reliable material to increase mechanical strength of the FRC. On this basis, this study aims to determine the effect of silk fiber concentration on the flexural strength of FRC. Fiber reinforced composite made of Bis-GMA/TEGDMA/ UDMA resin (CharmFil®, DenKist, Korea), hydroxyapatite (Bioceramic Laboratory, DTMI UGM) and silk fiber (Perhutani, Pati) were divided into three groups. Each group contained different silk fiber concentrations which were 1%, 5% and 10%. The flexural strength test was performed with 3-point bending test according to ISO 10477. The result showed that FRC with silk fiber 1%, 5% and 10% respectively had flexural strength of 61.21 ± 8.43 MPa, 62.97 ± 3.92 MPa and 85.01 ± 7.71 MPa. The result of one way ANAVA analysis showed that mean of FRCs flexural strength were significantly different between one treatment group to another. Thus, it is conclusive that silk fiber has a significant effect on FRCs flexural strength. The addition of 10% concentration of silk fiber is proven to increase FRCs flexural strength.
Streamline characteristics using the computational fluid dynamic analysis in the flow of 18% EDTA irrigation solution to remove Ca(OH)2 Muryani, Anna; Dharsono, Hendra Dian Adhita; Zuleika, Z.; Moelyadi, Ing. Mochammad Agoes; Prisinda, Diani
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.30886

Abstract

The remaining calcium hydroxide (Ca(OH)2) medicaments in the root canal wall can block the penetration of filling medicaments to the dentine tubule and cause the failure in the root canal treatment. One of the ways in cleaning the root canal wall from the remains of (Ca(OH)2) is by using 18% Ethylene Diamine Tetra Acetic Acid (EDTA) irrigation solution. The cleanliness of the root canal can be examined using the Computational Fluid Dynamics (CFD) analysis. The aim of this research was to see the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with 18% EDTA irrigation by means of CFD analysis. This is a descriptive-explorative research. Having been validated with the experimental research using test specimen in the form of block resin made in accordance with the characteristics of root canal, the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with the 18% EDTA irrigation was analyzed using CFD method. The irrigation needle used was the side-vented type with the position of 3 mm from the tooth apical. The results of the research showed the conformity between the result of experimental research and CFD research. One of the results of this research was the characteristics of streamline of 18% EDTA in the root canal showing a unique behavior due to the characteristics of the form of side vent irrigation needle. The irrigation flow in the coronal area of the inlet (side vent irrigation needle) showed a low velocity causing the more fluid flowing out from the inlet went to apical rather to the outlet (root canal orifice). In conclusion, this research showed that 18% EDTA solution indicated the conformity of validation results between experimental research and CFD research in the frames of 5, 10, 15, and 20 secs observed from experimental research with the of frame 0,010, 0,099, 0,150 and 0,410 secs as observed from CFD research.
Evaluation of surface roughness of the bracket slot before and after burning Jannah, Raudhatul; Thahar, Bergman; Mardiati, Endah; Evangelina, Ida Ayu
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.28387

Abstract

Slot bracket base is one important element to enable sliding movement of the archwire. Bracket reconditioning by means of burning changes the surface roughness of slot bracket base. The heat above 450 °C shows microstructure changes of the slot surface. On this basis, the present study aims to evaluate surface roughness changes of the slot bracket base before and after reconditioning by means of burning. Sixteen premolar brackets standard edgewise were divided into two groups each consisting of eight brackets. These two groups were tested at three different points using Scanning Electron Microscopy (SEM), with 3000 times of magnification. Subsequentl,group A was burned for 5 seconds (approximately 450 °C) and group B was burned for 10 seconds(approximately 643 °C). After treatment,these two groups were tested for the second time using Scanning Electron Microscopy (SEM), with 3000 times magnification and the images were analyzed using a discrete scale quantitative classification. Afterwards, the data were analyzed using ANOVA (p≤0.05) and post hoc analysis. The result showed that surface roughness of the slot bracket base burned for 10 seconds experienced more significant change than that of bracket burned for 5 seconds and that of the control bracket(p≤0.05).Bracket reconditioning using burning either for 5 to 10 seconds change the surface roughness of the slot bracket base.
Inhibition activity of Robusta coffee beans polyphenol extract on the production of TNF-α neutrophil cells Ermawati, Tantin; Meilawaty, Zahara; Harmono, Happy
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.28352

Abstract

Polyphenols are one of the active substances in the Robusta coffee beans with various benefits for humans’ health including anti-inflammation. neutrophil cell (polymorphonuclear PMN) plays a significant role as the primary immune response against foreign agent. Inflammatory response is characterized by the production of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α). Hence, the purpose of this study is to determine anti-inflammation capacity of Robusta coffee beans polyphenols extract on the TNF-α production in neutrophil cells. Neutrophil was derived from human peripheral venous blood by means of gradient density method. One hundred microliter of neutrophil  (1,5x103 cell) was incubated with 3.13%, 6.25%, 12.5%, 25% polyphenol extract and exposed with 100 μl of Lipopolysaccharides (LPS) 25 mg/ml. The production of TNF-α were determined by ELISA. Data were analyzed by one way Anova. Polyphenols of Robusta coffee bean extract was shown to inhibit TNF-α production in LPS-exposed neutrophil cells compared to controls. Whereas, LPS-exposed neutrophil increased TNF-α production. The most effective concentration to inhibit TNF-α production was 12.5%. It was concluded that polyphenols of Robusta coffee beans has anti-inflammatory properties as indicated by its ability to decrease TNF-α levels.
Anti-inflammatory role of papaya seed extracts in inhibiting osteoclastogenesis of rats with Periodontitis Pusporini, Ratih; Basori, Ahmad; Krismariono, Agung
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.26829

Abstract

Some previous studies mentioned that papaya seed extract has anti-inflammatory activity because it contains polyphenols including phenolic and flavonoid acids. Polyphenols of the papaya seed extracts are known to inhibit the severity of periodontitis. It can prevent the release of excessive osteoclastogenesis which may promote bone resorption aggravating periodontitis. Hence, this study aims to determine the role of papaya seed extract in reducing the number of osteoclasts in rats with periodontitis. Twenty-eight male Wistar rats were divided into 4 groups of induced periodontitis using lipopolisakarida (LPS) Porphyromonas gingivalis (P. Gingivalis) and followed by wire ligature in “8” placed around the mandible mandibular incisor. The first group was given standard food without any papaya extract, while each rat in three other groups with periodontitis (P2, P3, P4) was given with Papaya seed extracts of 200 mg/kgBW, 300 mg/kgBW, and 400 mg/kgBW. Seven rats in one group were not induced by periodontitis and were fed with standard food as a control group. On the 30th day, the rat was sacrificed, and its transverse pieces of alveolar bone around the mandibular incisor were stained with Hematoxylin-Eosin. The data analyzed by one way ANOVA indicated a significant result. Thus, it is conclusive that extract of papaya seed ethanol inhibited osteoclastogenesis in Wistar rats induced with periodontitis.
Effect of jackfruit seed fortification on high calcium milk on bone collagen density Ayub, Aulia; Adinsyah, Fun Nagede; Putri, Arum Trisnaningtyas Sugiyanto; Fitriani, Presty Dwi; Martasuri, Risma; Haniastuti, Tetiana
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.25979

Abstract

Osteoporosis is a bone disease characterized by reduced bone mass and damage to the microarchitecture of bone tissue. One of osteoporosis types is primary type-1 osteoporosis or postmenopausal osteoporosis due to estrogen deficiency. Calcium is an important mineral that plays a central role in the formation of bone strength structure. Jackfruit seeds (Artocarpus heterophyllus) contain isoflavones that can replace the role of estrogen in the body. Fortification of jackfruit seeds and high calcium milk can be a potential alternative for the treatment of postmenopausal osteoporosis. The purpose of this study was to determine the effect of drinking high calcium milk fortified with jackfruit seed extract (Artocarpus heterophyllus) on alveolar bone collagen fiber density of menopause induced rats. Twenty-five Sprague dawley rats were included in this study. Twenty Sprague dawley rats were ovariectomized, while 5 rats were left untreated (normal control group). Seven days after ovariectomy was performed, 5 rats were treated with fortification of jackfruit seed extract and high calcium milk, 5 rats were treated with jackfruit seed extract, 5 rats were treated with high calcium milk, and 5 rats were left untreated (ovariectomized control group). The treatments were done for 28 days. The rats were euthanized and the alveolar bone was taken and processed for a histological specimen. Alveolar bone collagen fibers were observed under a binocular microscope with 100 magnification. Kruskal-Wallis result showed there was a significant difference in the alveolar bone collagen fiber density among these groups (p<0.05). Mann-Whitney U Test showed a significant difference (p<0.05) in the alveolar bone collagen fiber density of the rats treated with fortification of jackfruit seed extract and high calcium milk compared to the group treated with jackfruit seed extract, that with high calcium milk, or ovariectomized control group. No significant difference in alveolar bone collagen fiber density was found between the group treated with fortification of jackfruit seed extract and high calcium milk and the normal control group (p>0.05). In conclusion, consuming high calcium milk fortified with jackfruit seed extract increases the density of alveolar bone collagen fibers.
Effect of Bombyx mori silk-fiber volume on flexural strength of fiber-reinforced composite Fransiska, Aria; Sunarintyas, Siti; Dharmastiti, Rini
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.25186

Abstract

Dental glass fiber is one of dental synthetic fibers that are widely used in dentistry as a dental resin reinforcement, such as in dentin replacement material. The availability of glass fiber is limited in Indonesia because it must be imported and relatively expensive. Bombyx mori silk-fiber is one of the strongest natural fiber derived from silkworm cocoon processing. Silk-fiber is used in medical applications as a post-surgical sutures, scaffolds for tissue engineering and drug delivery. The purpose of this study was to evaluate the effect of Bombyx mori silk-fiber volume on the flexural strength of fiber-reinforced composite (FRC). We used Bombyx mori silk-fiber (Perhutani Pati, Central Java, Indonesia) and flowable composite (Charmfil flow, Denkist, Korea) in this study. The FRC samples were divided into 4 groups consisting of fiber volumes of 0%, 5%, 10% and 15% (n = 4). Tests of flexural strength were performed according to ISO 4049. The results were analyzed using one way ANOVA (p<0.05). The study showed that the means of the flexural strength (MPa) of Bombyx mori silk-fiber FRC for volume of 0%, 5%, 10% and 15% were 149.2 ± 5.5; 127.6 ± 3.8; 110.9 ± 3.5; 71.2 ± 4.2. One-way ANOVA test showed that the means of FRC flexural strength on the four groups’ silk-fiber Bombyx mori volumetric were significantly different (p<0.05). This study concluded that Bombyx mori silk-fiber volumetric influences the flexural strength of fiber- reinforced composite. An increase in Bombyx mori silk-fiber volume decreases the flexural strength of FRC because there is a small gap due to the weak interfacial bonds between dental flowable composite and Bombyx mori silk-silk-fiber.
Effect of A’yun’s predictor software on the behavior, saliva pH, and PHPM index A'yun, Quroti; Subekti, Ani
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.23722

Abstract

Health Research in 2013 showed that 24.8% of children with health problems and dental caries index reached 1.4. A’yun’s Predictor Software (APS) was a new tool to describe the interaction of various factors that play a role in the caries process. The program was developed to establish a better understanding of the multi-factorial aspects of dental caries in children,as well as a guide to improve behavior to reduce the risk of new caries. This study in children aged 10-12 years in SDN Baturan I,SDN Baturan II,SDN Mayangan and SD Muhammmadiyah Trini in Sleman,Yogyakarta.Sampling technique was simple random sampling. This type of research was quasi-experimental control group(pretest and posttest design with control group). The independent variable was oral health education with APS and the dependent variable was the behavior of dental health maintenance oral and mouth, salivary pH and PHPM (Personal Hygiene Performance-Modified)index.Results data were analyzed by t-test. Research shown that there were significant differences between the treatment group and control group on the behavior of the maintenance of oral health of children and PHPM index (p <0.05), while the pH of saliva there was not to be difference (p> 0.05). The conclusions of this study was significant APS application on dental health maintenance behavior oral and mouth, salivary pH, and the index PHPM in school children.

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