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INDONESIA
Indonesian Journal of Tropical and Infectious Disease
Published by Universitas Airlangga
ISSN : 20851103     EISSN : 23560991     DOI : -
Core Subject : Health, Science,
This journal is a peer-reviewed journal established to promote the recognition of emerging and reemerging diseases specifically in Indonesia, South East Asia, other tropical countries and around the world, and to improve the understanding of factors involved in disease emergence, prevention, and elimination. The journal is intended for scientists, clinicians, and professionals in infectious diseases and related sciences. We welcome contributions from infectious disease specialists in academia, industry, clinical practice, public health, and pharmacy, as well as from specialists in economics, social sciences and other disciplines.
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Articles 6 Documents
Search results for , issue " Vol 7, No 5 (2019)" : 6 Documents clear
OVERVIEW OF NUCLEAR FACTOR-KB (NF-KB) AND NON-STRUCTURAL PROTEIN 1 (NS1) IN PATIENTS WITH DENGUE FEVER IN PREMIER HOSPITAL, SURABAYA Budiutari, Ni Nyoman; Dachlan, Yoes Prijatna; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 5 (2019)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (656.08 KB) | DOI: 10.20473/ijtid.v7i5.9955

Abstract

Dengue fever (DF) is an acute viral fever caused by RNA virus that is transmitted by Aedes aegypti and Aedes albopictus mosquitoes. DF is also called viral arthropod-borne disease and is accompanied by headaches, joint and muscle pain. The main target of dengue infection is macrophages or monocytes and dendritic cells (DC). Infected DC is caused the viral replication and the endocytosis into endosomal, easier, thus inducing the activation of NF-ĸB transcription factor to produce proinflammatory cytokines such as Tumor Necrosis Factor-α (TNF-α), Interleukin-1 (IL-1), IL-6, IL-12 and chemokine. NF-kB is one of the transcription factors involved in the regulation of the expression of various cytokines, chemokines and anti/pro-apoptotic proteins during infection and act as indicator of disease severity. Infected DC cells are secreted NS1 protein which is the co-factor needed for viral replication and can be detected in the first eight days. The level will be higher in the initial phase of fever. The purpose of this study was to analyze the description of NF-kB and NS1 levels in the serum of patients with dengue fever through observational analytic studies through a cross-sectional approach. This study was done on 40 patients with dengue fever and 10 healthies people as negative controls. NS1 was analyzed in serum of Panbio rapid test and NF-kB level were measured by sandwich ELISA. The results are showed positive and negative NS1 results in dengue fever patients. The average NF-kB serum level in dengue fever patients was found to be higher than the control. NF-ĸB level in negative NS1 was higher than the NS1 positive group. It is showed that NS1 is detected both in the acute phase. The detection of NF-ĸB is showed the involvement of transcription factors in the development of dengue virus infection and has a protective role for host cells.
ANTI-DENGUE TYPE 2 VIRUS ACTIVITIES OF ZINC (II) COMPLEX COMPOUNDS WITH 2-(2,4 -DIHYDROXYPHENYL)-3,5,7-TRIHYDROXYCROMEN-4-ONE LIGANDS IN VERO CELLS Sucipto, Teguh Hari; Setyawati, Harsasi; Churrotin, Siti; Amarullah, Ilham Harlan; Sumarsih, Sri; Wardhani, Puspa; Aryati, Aryati; Soegijanto, Soegeng
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 5 (2019)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (777.006 KB) | DOI: 10.20473/ijtid.v7i5.10851

Abstract

Dengue virus (DENV) is a disease that is transmitted through Aedes aegypti and Aedes albopictus mosquitoes, and is spread in tropical and sub-tropical regions. Now, dengue or antiviral vaccines for humans do not yet exist, but there are great efforts to achieve this goal. Complex compounds are reported to fungicidal, bactericidal and antiviral activity. Antiviral activity against DENV is an important alternative to the characterization and development of drugs candidate. The purpose of this study was to study zinc(II) compounds with 2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxycromen-4-one ligand on DENV-2 replication in Vero cells. Vero cell lines (African green monkey kidney) was used in this study, maintained and propagated in Minimum Essential Eagle Medium containing 10% fetal bovine serum at 37°C in 5% CO2. The activity of dengue virus was carried out by enzyme-immunosorbent assay (ELISA) method and CellTiter96® Non-Radioactive Proliferation. The value of activity inhibition (IC50) of complex compounds with variations of mol metal: ligand 1:2, 1:3, and 1:4 against dengue virus type 2 (DENV2) was 2.44 μg/ml, 2.75 μg/ml, respectively and 2.00 μg/ml, also the toxicity value (CC50) of complex compounds with variation mol metal: ligand 1:4 for Vero cells is 3.59 μg/ml. The results of this study were indicate that these properties have been shown to inhibit anti-dengue type 2 virus (DENV-2), but are also toxic in Vero cells. Including previous study about complex compound interaction with dengue virus type 2 activity, Zn(II) more reactive compound then Cu(II), and Co(II). The comparison with Cu(II) complex compound, it has been revealed that Co(II) and Zn(II) is more toxic, was found to be nontoxic to human erythrocyte cells even at a concentration of 500 μg/ml.
COMBINED TARGET SITE VGSC MUTATIONS PLAY A PRIMARY ROLE IN PYRETHROID RESISTANT PHENOTYPES OF Aedes aegypti AS DENGUE VECTOR FROM PALU CITY, CENTRAL SULAWESI Umniyati, Sitti Rahmah
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 5 (2019)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1124.721 KB) | DOI: 10.20473/ijtid.v7i5.10384

Abstract

It has been reported that Aedes aegypti mosquitoes in Palu City had been resistant to cypermethrin insecticide but the resistance mechanism is not well known. This study aimed to determine the resistance status of Ae. aegypti to cypermethrin and whether the mutation of voltage-gated sodium channel (VGSC) was associated with pyretroid resistance in high and low dengue endemic areas in Palu City. Aedes aegypti collected from each village was reared to adult and assayed for susceptibility test to cypermethrin using the CDC bottle bioassay method. PCR primers of AaSCF1 and AaSCR4 were used for screening of IIS6 VGSC gene mutation. PCR primers of AaSCF7 and AaSCR7 were used for screening of IIIS6 VGSC gene mutation. For an identification of mutation sites were sequenced and aligned to Gene bank (access No. AB914689 and AB914690) for IIS6 VGSC and Gene bank (access No. AB914687 and AB914688) for IIIS6 VGSC gene mutation. The susceptibility status of Ae. aegypti to cypermethrin was resistant in high dengue endemic areas and moderately resistant in low dengue endemic areas. It was found double point mutation at S989P and V1016G in Ae. aegypti from high and low dengue endemic areas in Palu City and there was a single point mutation only in high dengue endemic area at target site V1016G. Aedes aegypti from both high and low dengue endemic areas were resistant to cyperpethrinn and the two alleles had a major role in the occurrence of cypermethrin resistance in Palu City.
ACID-FAST BACILLI CONVERSION OF BEIJING AND NON-BEIJING STRAIN OF PULMONARY TUBERCULOSIS IN SOUTH SULAWESI Mertaniasih, Ni Made
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 5 (2019)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1454.76 KB) | DOI: 10.20473/ijtid.v7i5.9670

Abstract

Beijing strains are a major part of the Mycobacterium tuberculosis Asian phylogenetic lineage. Beijing strains represent about 50% of all TB strains in East Asia and at least 13% of strains worldwide. Beijing strain of Mycobacterium tuberculosis is presumed as the factor of the increase in bacteria virulence and drug resistance, and the contributor in treatment failure. The aim of this study was to analyze the association between acid-fast bacilli conversion with strain genotipe Beijing and non-Beijing of pulmonary tuberculosis in South Sulawesi. The design of research was observational analytic with prospective approach. The sampling technique used consecutive sampling. Data were taken from active pulmonary tuberculosis patients’ medical record in Balai Besar Kesehatan Paru Masyarakat Makassar (Pulmonary Health Center of Makassar) and Community Health Center in Gowa Regency, South Sulawesi from March to June 2018. Collected sputum samples were screened for AFB and identified as Beijing strain and non Beijing strains using Multiplex PCR in Tropical Disease Institute of Universitas Airlangga. The results is showed that the characteristics of the respondents consisted of 12 respondents (33.3%) aged 56-65 years, 25 respondents (69.4%) men and 28 respondents (77.8%) had low category gradation of AFB smear. Univariate analysis showed 6 respondents (16.7%) with Beijing strains, 30 respondents (83.3%) with non-Beijing strains, 32 respondents (88.9%) conversion sputum AFB and 4 respondents (11.1%) non conversion sputum AFB. Bivariate analysis with Chi-Square statistical test shows that p value 0.022 < 0,05, that means there was association of Beijing strains with BTA conversion. Microscopic examination of BTA can be used to monitor and evaluate the treatment of new pulmonary TB patients undergoing treatment and the Beijing Mycobacterium tuberculosis strain has a significant correlation with the treatment failure of anti-tuberculosis drugs in South Sulawesi.
DETECTION OF TUMOR NECROSIS FACTOR- (TNF- ) GENE PROMOTERS POLYMORPHISM AMONG LIVER CIRRHOSIS PATIENTS WITH CHRONIC HEPATITIS B VIRUS (HBV) INFECTION IN SURABAYA, INDONESIA Wungu, Citrawati Dyah Kencono; Amin, Mochamad; Ruslan, S. Eriaty N.; Purwono, Priyo Budi; Kholili, Ulfa; Maimunah, Ummi; Setiawan, Poernomo Boedi; Lusida, Maria Inge; Soetjipto, Soetjipto; Handajani, Retno
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 5 (2019)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1302.693 KB) | DOI: 10.20473/ijtid.v7i5.7275

Abstract

Polymorphisms in TNF-α gene promoter region are known of its role in the production of TNF-α which may influences the pathogenesis of liver disease. SNPs in positions 238 and 308 of TNF-α gene promoters may affect the production of these cytokines. This study was aimed to detect Single Nucleotide Polymorphism (SNP) on -238 and -308 positions in the TNF-α gene promoter among liver cirrhosis patients with HBV infection in Surabaya, Indonesia. This was descriptive exploratory research with cross sectional study design using serum liver cirrhosis patients with HBV infection in Endoscopy Outpatient Clinic Dr. Soetomo General Hospital, Surabaya from April-May 2017. SNPs at -238 and -308 on TNF-α gene promoter (rs361525 and rs1800629 respectively) were detected using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with primers specific for the TNF-α promoter region and restriction enzymes NcoI and MspI. The genotypes of TNF-α gene promoter were assessed according to the length of the fragments produced in RFLP. Serum TNF-α levels was measured by commercial ELISA. In this study, as much as 149 positive HBsAg patients was found in Endoscopy Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya. From those amount, as much as 30 liver cirrhosis patients with positive HBsAg were obtained. From 2/30 (6.7%) patients showed the GA heterozygote SNP either position -238 or -308. No patient had the AA genotype. Median blood TNF-α level in women (38 ng / L) was higher than in men (33 ng / L). TNF-α levels in patients with GA heterozygote genotype at -238 and -308 in this research was not different than wild-type (GG genotype). Among patients with liver cirrhosis due to chronic HBV infection in Surabaya, Indonesia, Surabaya, we found GA polymorphisms the TNF-α promoter gene at positions -238 and -308 in 6.7% patients, and did not find homozygous AA polymorphisms. Further studies including larger numbers of patients from various ethnic backgrounds in Indonesia are needed to provide robust data on TNF-α gene promoter polymorphisms and their role in the pathogenesis of liver cirrhosis with HBV infection in this country.
MICROBIAL PATTERN AND ANTIBIOTIC SUSCEPTIBILITY IN PEDIATRIC INTENSIVE CARE UNIT DR. SOETOMO HOSPITAL, SURABAYA A.A.W., I Wayan Putra; Irwanto, Irwanto; Dharmawati, ira; Setyaningtyas, Arina; Puspitasari, Dwiyanti; Wahyu, Agung Dwi; Kuntaman, Kuntaman
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 5 (2019)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (520.415 KB) | DOI: 10.20473/ijtid.v7i5.5737

Abstract

Gram-negative bacterial are known as common pathogen caused infection in Pediatric Intensive Care Unit (PICU). Microbial Pattern and Antibiotic Susceptibility are needed as clinical data for selected appropriate antibiotic therapy. In PICU Dr. Soetomo hospital until now still lacking of Microbial Pattern and Antibiotic Susceptibility data. This descriptive study is to recognized Microbial Pattern and Antibiotic Susceptibility in PICU patients from blood, urine, sputum, stool, cerebrospinal fluid, endotracheal tube, pus swab and pleural fluid culture specimens. Patients whose admitted into PICU without signs of infections were excluded from the study. The inclusion criteria are patients with sign infection as follows: fever < 36,5°C or > 37.5°C, leukocyte < 4000/mm3 or > 10000/mm3, marker infections CRP >10 mg/L or PCT >0,3 ng/mL, bradycardia or tachycardia, tachypnea, infiltrates on chest X-ray, turbid urine, dysuria, thrombophlebitis, abdominal pain or tenderness, and mucous or skin lesion. Medical record data from 2011 to 2016, revealed 1138 patients had positive microbial culture result, wherein positive result came from blood 44.46%, urine 19.15%, sputum 11.59%, stool 8.96%, cerebrospinal fluid 7.50%, endotracheal tube 4.04%, pus swab 2.89%, and pleural fluid 1.41%. The microorganisms found in PICU Dr. Soetomo was dominated with gram negative bacteria. Commonest bacterial that recognized from blood was B. cepacea, urine was E. coli, sputum was P. aeruginosa, Stool was E. coli, Cerebrospinal fluid was S. cohnii, endotracheal tube was K. pneumoniae ESBL, pus swab was S. aureus, and pleural fluid was S. maltophilia. Both gram-negative bacteria and gram-positive bacteria isolates revealed multiple drug resistance to commonly used antibiotic, but still had good susceptibility for antibiotic such as; amikacin, cefoperazone-sulbactam, linezolid, vancomycin and carbapenem group.

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