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The Journal of Pure and Applied Chemistry Research
Published by Universitas Brawijaya
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Articles 15 Documents
Search results for , issue " Vol 7, No 2 (2018): Edition May-August 2018" : 15 Documents clear
Study of Antocyanins Activity from Purple Sweet Potato for Reducing Apoptotic Cells Expression of The Cerebellum On Ischemic Stroke Rats Dewi, Latifah Tribuana; Adnyana, Made Oka; Mahdi, Chanif; Prasetyawan, Sasangka; Srihardyastutie, Arie; Aulanni’am, Aulanni’am
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.348

Abstract

This study aims to determine anthocyanin antioxidant performance with a dose of 2 cc/day based on the expression of apoptotic cells on ischemic stroke rats cerebellum. Research was conducted using ischemic stroke rats (Rattus norvegicus) that prepared by ligated for 3 h on the Common Carotid Artery (CCA) and External Carotid Artery (ECA) followed by reperfusion that commonly known as MCAO (Middle Cerebral Artery Occlusion). The anthocyanin extract was characterized by LC-MS and its IC50 was measured by DPPH method. The rats were divided into five groups 1) negative control; 2) reperfusion 1 h; 3) reperfusion 72 h; 4) reperfusion 24 h, with anthocyanin therapy; 5) reperfusion 72 h, with anthocyanin therapy. The results of LC-MS showed that anthocyanin from purple sweet potato extracts contained Petunidin-3,5-O-diglucoside (Pt-DG) with an IC50 value of 22.16 μg/mL, categorized as very strong antioxidant. The results showed that apoptotic cells expression of cerebellum decreased significantly (p<0.01) after 72 h reperfusion with anthocyanins therapy until 2.42%. The current work proved that anthocyanin extract effectively suppresses the apoptotic cell’s expression of the cerebellum on stroke ischemic rats.
Calcium Oxide Catalyst Based on Quail Eggshell for Biodiesel Synthesis from Waste Palm Oil Mohadi, Risfidian; Sueb, Abi; Anggraini, Kiki; Lesbani, Aldes
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.390

Abstract

Calcium oxide decomposed from quail eggshell was used as catalyst for biodiesel synthesis from waste palm oil. Prior to being used, the quail eggshell was decomposed at 600-1100 oC to form calcium oxide and it was characterized by X-Ray measurement, FTIR and SEM spectroscopy to analyze the functional groups and the surface morphology, followed by N2 adsorption desorption methods to determine the surface area. The results of X-Ray analysis powder pattern show that the decomposition of quail eggshell at 900 oC gave calcium oxide that has similar characteristic to the standard CaO from Joint Committee of Powder Diffraction Standard (JCPDS). The FTIR spectrum indicated vibration of calcium oxide from quail eggshell have a similar pattern with the calcium oxide of the standard. The SEM analysis showed that morphology of quail eggshell was changed after decomposition at 900 oC and it have the mesoporous structure.  The biodiesel from waste palm oil was synthesized using CaO catalyst from quail eggshell decomposed at 900 oC. The biodiesel product has density of 0.86 g/cm3, viscosity of 5.50 mm2/s, free fatty acid of 0.56 mg/KOH, and iodine number of 60.49 g I2/100g, respectively. All those biodiesel characteristics meet to the biodiesel standard by the Indonesian National Standard (SNI).
Xylanase Production from Trichoderma viride and its Immobilization on the Sea Sands Matrix Sutrisno, Sutrisno; Roosdiana, Anna; Suratmo, Suratmo
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.408

Abstract

The study was aimed to investigate the production and characterization of xylanase from Trichoderma viride by determining the optimum condition of xylanase immobilization using sea sand matrix. The parameters were shaking time variations of 1, 2, 3, 4, and 5 hours and xylanase concentration of 0.5, 1.5, 2.5, 3.5, and 4.5mg/mL. Xylanase was purified by ammonium sulfate using the optimum fraction of 40-80%. The optimum shaking time for immobilization of xylanase was reached in 4 h, and the optimum xylanase concentration was obtained in 4.5 mg/mL, with immobilized xylanase activity of 118.55 µg.g-1.min-1 and adsorbed xylanase amount in sea sand matrix of  20.51 mg. Immobilized xylanase can be used up to 4 times, with the percentage of 62.47%. The surface characterization of immobilized xylanase was conducted using SEM.The FT-IR result obtained peaks at wavenumber 3431.13 and 875.62 cm-1 which indicatedthe existence of N-H bond from enzyme and the sea sand bind with enzyme, respectively. 
Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS Nurmaida, Nurmaida; Darusman, Latifah K; Rafi, Mohamad; Heryanto, Rudi
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.374

Abstract

Tabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ultrasonication techniques with methanol (MM and UM) and ethanol (ME and UE) were used to extract the F. deltoidea metabolites. About 70 metabolites were identified by using UPLC-QTOF-MS/MS in negative ion mode. The amounts of metabolites found in each extract were different, i.e., 45 metabolites in MM, 64 metabolites in UM, 42 metabolites in UE and 41 metabolites in ME. MS/MS could further tentatively identify 16 metabolites. The identified compounds belonged to the class of flavonoids and phenolic acid. Also, we conducted an antioxidant activity by using DPPH method on each extract to determine its potency as an antioxidant. The highest antioxidant activity was exhibited by UM extract (IC50 71.93 ppm) may be due to the number of metabolites in UM extract which was higher than the other extract based on the detected metabolites.
Preparation and Characterization of Highly Water Soluble Curcumin – Dextrose Cocrystal Kho, Katherine; Nugroho, Denny; Sugih, Asaf Kleopas
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.401

Abstract

Curcumin is a natural food colorant isolated from rhizomes of turmeric (Curcuma longa). Despite its many favorable properties, curcumin is practically insoluble in water and relatively unstable, thus limiting its application. In this research, a potential method to improve curcumin solubility and stability, i.e. cocrystallisation of curcumin with dextrose was investigated.. The effect of curcumin content in the cocrystals on solubility and yield of the product was studied. The morphology of the cocrystals was observed using SEM. In addition, stability in different pH range was investigated.  Crystal structure and curcumin – dextrose interaction were analyzed using FT-IR spectra and DSC thermograms. The result shows that curcumin – dextrose cocrystal is a potential food colorant that could be applied to water – based food at various pH range. 
Application of Modified Clay Catalysts in Oligomerization of Light Olefins Toktassyn, Raila; Utelbayev, B.T.
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.404

Abstract

The oligomerization of light olefins to high-octane clean engine fuels is one possible way to solve increased requirements for motor fuels. This paper presents a study of catalytic oligomerization of C2-C4 olefins on Ru-Fe supported pillared clay catalyst with different metallic quantity. The prepared catalysts were characterized by X-ray diffraction, N2 adsorption-desorption at 77K, BET and BJH methods. The BET and BJH methods showed the pillared clay had significantly increased surface area and when an appropriate amount of Ru and Fe were added, the bimetallic alloys were uniformly dispersed on the modified clay surfaces and had an average mesopore size of 4.1 nm. It is indicated that excess metal oxides blocked the surface of bimetallic RuFe/MMC system, resulted in decreased catalytic activity. The low metal content catalysts showed higher oligomerization activity and increasing the Fe content gave selectivity to isomerization reactions.
The Toxicity Effect of Organophosphate (Diazinon) towards Duodenum Histopathology and The Activity of Superoxide Dismutase (SOD) Serum in Rats (Rattus Norvegicus) Ulansari, S. Efi; Roosdiana, Anna; Oktavianie A. P., Dyah Ayu
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.387

Abstract

Diazinon is an organophosphate insecticide that can inhibit acetyl-cholinesterase competitively and increase the production of Reactive Oxygen Species (ROS). ROS affect the activity of antioxidant enzymes such as superoxide Dismutase (SOD), and simultaneously ROS cause damage to the cells and duodenum tissue. The toxicity effect of diazinon was investigated by measuring the activity of SOD serum and histopathology of the duodenum in rats (Rattus norvegicus). The activities of SOD serum were measured by using spectrophotometry, and histopathology changes of the duodenum were observed by using HE staining. Rats were divided into 4 groups, negative control group (K-), P1, P2, and P3 groups which were administrated with diazinon for 8 weeks orally with a dose of 20 mg/kgBW, 40 mg/kgBW, and 60 mg/kgBW respectively. The results showed that administration of diazinon orally decreased SOD activity significantly and caused damage of duodenal villi such as hyperplasia of epithelial cells, epithelial erosion, fatty degeneration, hyperplasia of Liberkuhn gland cells, and hemorrhage in the lamina propria.
Fabrication of Hydrophobic Indonesia Bamboo Modified by Octa Fluoro 1-Pentanol (OFP) Based on TiO2 Thin Film for Self-cleaning Application Wellia, Diana Vanda; Mustaqimah, Arifah; Wulandari, Wulandari; Zulhadjri, Zulhadjri; Syukri, Syukri; Pratiwi, Nurul
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.396

Abstract

The ultra-hydrophobic surface on Indonesia bamboo timber has been successfully prepared using OFP (2,2,3,3,4,4,5,5-octafluoro-1-pentanol) as a modifier agent. The hydrothermal method has been used to fabricate anatase TiO2 film followed by OFP modification. Maximal water contact angle of 123ο has been obtained for the composition of 10 mL of OFP and 15 mL of 2-propanol (B-T-OFP10). XRD analysis showed the existence of pure anatase TiO2 film on bamboo timber, confirmed by EDS result. SEM image of a TiO2-coated and a typical ultra-hydrophobic bamboo timber revealed irregular aggregates of spherical TiO2 on the surface and compact ultra-hydrophobic  surface, respectively. The optimum sample (B-T-OFP10) showed excellent mechanical stability, self-cleaning property, and flame retardancy compared to pure bamboo timber. 
L-Histidine-Modified Silica from Rice Husk and Optimization of Adsorption Condition for Extractive Concentration of Pb(II) Nurhajawarsi, Nurhajawarsi; Rafi, Mohamad; Syafitri, Utami Dyah; Rohaeti, Eti
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.402

Abstract

A new chelating agent, L-histidine-modified silica from rice husk (LHSRH), was prepared to increase the adsorption capacity and selectivity for Pb(II). LHSRH was synthesized by immobilizing L-histidine on silica from rice husk (RH) modified with 3-aminopropyltrimethoxysilane (APTMS). Silica from rice husk (SRH) was synthesized via precipitation process by adding hydrochloric acid solution to rice husk ash (RHA). The RHA was subsequently destructed with sodium hydroxide and heated to obtain sodium silicate (Na2SiO3). SRH was characterized by Fourier transform infrared spectroscopy and x-ray diffraction. The LHSRH was used further to adsorp Pb(II) metal ion. The pH range, amount of adsorbent, and adsorption time were optimized by response surface methodology. The optimum condition for the adsorption of Pb(II) was pH 5, an amount of adsorbent 0.1 g; and adsorption time 15 minutes. The adsorption capacity for Pb(II) ion was found to be 62.5 mg/g. The adsorption behavior of the matrix followed the Langmuir’s model.
Effects of Ruellia tuberosa L. Root Extract on the Pancreatics of Diabetic Rat Asadullah, Muhammad; Srihardyastutie, Arie; Aulanniam, Aulanniam
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2018.007.02.395

Abstract

Diabetes mellitus type 1 (DMT1) is an insulin deficiency disorder caused by pancreatic damage characterized by chronic hyperglycemia. Animal modeling of DMT1 using inducting Multiple Low Dose Streptozotocin (MLD-STZ) elevat levels of free radicals and inflammation in pancreatic organs which then damage β cells. Ruellia tuberosa L. is a plant containing triterpenoids from the class of antioxidant and anti-inflammatory flavonoids. This study used three groups of rat: negative control group, positive control and 240 mg/kg bw therapy group. Study are based on changes MDA levels, blood glucose and histologic of pancreas. MDA level determining with TBA method, glucose levels using EasyTouch GCU and histopalogy of pancreas using Hematoxylin-Eosin staining. Statistical analysis of MDA and blood glucose (α = 0.05) showed significant decreases in MDA and blood glucose levels. Based on the results of histologic observations of pancreas descriptively showed a decreas in cell damage on Langerhans island. Thus, the provision of root extract of Ruellia tuberosa L. can inhibit the damage in rat DMT1 model so that it can be used as therapy of diabetes mellitus.

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