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INDONESIA
Indonesian Journal of Animal and Veterinary Sciences
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
JITV (Indonesian Journal of Animal and Veterinary Science),  ISSN: 0853-7380 E-ISSN: 2252-696X is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of animal and veterinary science. It was first published in 1995. The journal has been registered in the CrossRef system with Digital Object Identifier (DOI) prefix 10.14334.
Arjuna Subject : -
Articles 6 Documents
Search results for , issue "Vol 24, No 3 (2019): SEPTEMBER 2019" : 6 Documents clear
Characterisation of M2e Antigenicity using anti-M2 Monoclonal Antibody and anti-M2e Polyclonal Antibodies Sumarningsih, Sumarningsih; Tarigan, Simson; Ignjatovic, Jagoda
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 3 (2019): SEPTEMBER 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i3.1987

Abstract

Matrix 2 ectodomain (M2e) protein is a potential antigen for detection of influenza A virus infection in vaccinated poultry (DIVA test). However the M2e antigenicity and immune response it induces in either humans or animals are poorly understood. Seventeen M2e peptides and sixteen recombinant M2e (rM2e) proteins with amino acid (aa) changes introduced at position 10, 11, 12, 13 14, 16, 18 and 20 were compared by western blot (WB) and enzyme-linked immunosorbent assay (ELISA) using mouse anti-M2 monoclonal antibody (mAb) 14C2, and anti-M2e peptide chicken and rabbit polyclonal antibody (pAb). The mAb 14C had the best discriminating power and indicated that all six positions contributed to the M2e antigenicity. Position 11 was the important immunodominant and affected Mab14C binding to a greatest degree. Changes in the adjacent position 14, 16 and 18 also influenced the binding, and it detected regardless of the method (WB or ELISA), or the antigen used (M2e peptide or rM2e). For chicken pAb and rabbit pAb, the immunodominant aa was position 10 and the antibody reaction was not affected by aa change at 11. The binding of rabbit pAb was also affected by changes at 14 and 16, which confirm the contribution of these positions to the M2e antigenicity. Position 10 was the only important position for the binding of chicken pAb to M2e. Overall, the study showed that the M2e antigenic sites are located between residues 10 – 18 and that aa changes at position 10, 11, 12, 14, 16 and 18 may all affect the antibody binding within the M2e protein.
Antimicrobial, Antioxidant and Anti-inflammation Activities of Fraction and Single Peptides Derived from Mare Milk Protein Kusumaningtyas, Eni; Subekti, Didik Tulus; Fitaningtyas, D FL
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 3 (2019): SEPTEMBER 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i3.1976

Abstract

Mare milk protein contains bioactive peptide which beneficial for human and animal health. Peptides in the fraction and single may show different activities.  The objectives of the study were to evaluate antimicrobial and anti-inflammation activities of the fraction and single peptide derived from mare milk protein. Antimicrobial assay was conducted by testing antibacterial and antifungal activities of fraction <3 kDa, peptide HPYFYAPELLYYANK, LVNELTEFAK and LANSLTEFAK against Escherichia coli and Candida albicans. Anti-inflammation effect was detected by interleukin 1-β (IL- 1β) and Tumor Necrosis Factor -α (TNF-α) production in mice after administration of Escherichia coli’s lipopolysaccharide (LPS) and combined with fraction or single peptide. The result showed that antibacterial and antifungal of fraction <3 kDa was higher than all of single peptide. This may because of synergistic interaction among peptide in the fraction which increase the activities. Fraction <3 kDa was also able to decrease production of IL-1β and TNF-α better than single peptide indicated its ability to decrease inflammation. Based on the results, antimicrobial and anti-inflammation activities fraction <3 kDa was better than single peptide. 
Model of Resources Development in Partnership System of Broiler Farming Business Amam, Amam; Fanani, Zaenal; Hartono, Budi; Nugroho, Bambang Ali
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 3 (2019): SEPTEMBER 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i3.1927

Abstract

Accessibility of resources in theory can affected the development of broiler farming in a region. This research was conducted with the objectives of: 1) to formulate indicators of resource use on the development of partnership system of broiler farming business, and 2) to find the model of the development of resources use accessibility to partnership system of broiler farming business. The research was conducted in August 2017 up to January 2018 in Malang Regency of East Java Province, Indonesia. 100 respondents as partnership system of broiler farmer was participated in this study who was determined by total sampling. The research variables consist of: a) financial resources, b) technology resources, c) physic resources, d) economic resources, e) environmental resources, f) social resources, g) human resources, and h ) business development. The data was analyze used by SEM with SmartPLS 2.0 analysis tool. The results indicate that: 1) The development of partnership system of broiler farming business is directly influenced by the financial resources of 2.421, the physic resources is 1.821, the economic resources 2.445, and the human resources 2.904, but the development of partnership system of broiler farming business direct are not affected by technology resources, environmental resources, and social resources; 2) The development of partnership system of broiler farming business is indirect with quality of human resources is influenced by the financial resources of 2.629, technology resources of 6.092, physic resources of 2.308, and economic resources of 2.312, but the development of partnership system of broiler business is indirectly with the quality of human resources are not influenced by environmental resources and social resources.
Retained placenta in Relation with Blood Components in Egyptian crossbred cattle Mourad, Reem S.
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 3 (2019): SEPTEMBER 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i3.2002

Abstract

          The present study was carried out in the veterinary units on 14 normal cows and 32 cows with Retained Placenta. Cows were chosen after about 6 to 12 hours from parturition in Shebin Elkom, Menoufia, Egypt.        Results show that there was very highly significant increase of concentrations of all blood biochemical components, macro elements and micro elements in normal cows than those in retained fetal membranes cows except in globulin, Sodium and Manganese.  On the other hand, plasma concentrations of biochemical components were highly significant between summer and winter in cholesterol, total protein and albumin but it wasn’t significant in glucose, globulin and A/G ratio. Moreover, there was very highly significant in plasma concentration between summer and winter in all macro elements except potassium and Ca/P ratio. Also, there was very highly significant in plasma concentration between summer and winter in all micro elements except Manganese. Meanwhile, plasma concentrations of biochemical components were not significant between parities except in A/G ratio. Furthermore, there was no significant in plasma concentration between summer and winter in all macro elements. But, there was very highly significant (P≤0.01) in plasma concentration of Cu and Cd between summer and winter, and it was significant (P≤0.05) in Fe and Se but, there was no significant in Co, Zn, Mn, and Mo.      The aim of this study was to determine blood plasma constituents and inorganic macro and micro elements in the case of retained placenta in crossbred cattle under Egyptian conditions.
Plasma Β-Endorphin and Cortisol Profiles around Periparturient Period at Stressful Conditions in Egyptian Buffalo Mourad, Reem S; Abed, Ha El-Hady; El-Malky, O M; Al-Gezery, I S
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 3 (2019): SEPTEMBER 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i3.2003

Abstract

The study determined the level of plasma β-endorphin and cortisol concentrations in peripheral blood circulation of buffalo cows suffering from reproductive disorders (dystocia and retained placenta) and weakness body condition score during periparturient period. Twenty multi-parous Egyptian buffalo cows at late pregnancy period were used for two months before parturition. β-endorphin concentrations were higher in buffalo suffering from reproductive disorders groups. Whereas, β-endorphin concentrations were 134.9±4.8 for retained placenta, 121.3±4.9 for dystocia, 114.2±8.4 for weakness and 113.5±6.5 pg/ml for control. In the closer period around parturition both of plasma β-endorphin and cortisol followed the same trend toward a gradually increased values during -2,-1days and zero time in all groups. A concomitant trend was noticed in β-endorphin and cortisol concentrations in postpartum period with reduce values were observed in all groups after parturition continued for month or more. Buffalo suffering from reproductive disorders were showed a high relative values in β-endorphin and cortisol concentrations. A significant differences (P<0.01) were observed between the experimental groups. Generally, buffaloes suffering reproductive disorders had a clear impact on blood plasma β-endorphin concentration around parturition process.The aim of this study was to determine the relationship between various reproductive disorders as a stress factors with plasma β–endorphin and cortisol in buffalo cows around parturition and changes in these parameters could be used as an objective measure of the stress associated labour. Stress has been hypothesized to be a cause of impaired reproductive efficiency. Stress may cause an overproduction of beta-endorphins and free radicals
Effect of Adding Insulin Transferrin Selenium (ITS) in the Medium on Maturation and Fertilization Rates of Bali Cattle Oocytes Iskandar, Hikmayani; Sonjaya, H; Yusuf, M
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 3 (2019): SEPTEMBER 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i3.2020

Abstract

The aim of this study was to determine the effect of adding Insulin Transferrin Selenium (ITS) in the medium on maturation and fertilization rates of Bali cattle oocytes. The ovarium of Bali cows were sliced to produce oocytes, then the collected oocytes were subsequently selected based on their quality. Those oocytes were maturated for 24 hours and then fertilized for 18 hours in the incubators of 5% CO2 with temperature of 38.5ºC. Oocytes were stained using 2% of aceto orcein, and observed under a microscope. This study was divided into four treatments at different addition of ITS in the medium and replicated four times, and analized using a Factorial Complete Randomized Design (CRD)at each treatment. The treatments consisted of P0 as control, P1 (5 ng/ml), P2 (10 ng/ml), and P3 (15 ng/ml). Parameters observed in this study were the stages of oocytes maturation rate that consisted of germinal vesicle (GV), germinal vesicle break down (GVBD), metaphase I (M-I), and metaphase II (M-II), and fertilization rate that consisted of absence of pronucleus (P0), 1 pronucleus (PN1), 2 pronucleus (PN2), and more than two pronucleus (>2PN). The results of this study showed that addition of ITS at 5-15 ng/ml did not effect maturation and fertilization rates of Bali cows oocytes.The conculision of the study is addition of ITS at 5 ng / ml produces the best level of maturation and for the best level of fertilization with addition ITS at 15 ng / ml.

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