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Ahmadi Riyanto
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medpub@litbang.deptan.go.id
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INDONESIA
Indonesian Journal of Animal and Veterinary Sciences
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
JITV (Indonesian Journal of Animal and Veterinary Science),  ISSN: 0853-7380 E-ISSN: 2252-696X is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of animal and veterinary science. It was first published in 1995. The journal has been registered in the CrossRef system with Digital Object Identifier (DOI) prefix 10.14334.
Arjuna Subject : -
Articles 11 Documents
Search results for , issue " Vol 6, No 4 (2001): DECEMBER 2001" : 11 Documents clear
Immunohistochemical study of Ito cells of spontaneous cholangiohepatitis in broiler chickens Handharyani, E; Ochiai, K.; Winarsih, W.; Harlina, E.; Estuninngsih, S.
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.05 KB) | DOI: 10.14334/jitv.v6i4.265

Abstract

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis and malformation of extrahepatic biliary tracts.Key words: Cholangiohepatitis, immunohistochemistry, ito cell. broiler chicken
Effects of Sallropus androgynus (katuk) leaf extract on growth, fat accumulation and fecal microorganisms in broiler chickens Santoso, U; Handayani, E; ., Suharyanto
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.627 KB) | DOI: 10.14334/jitv.v6i4.244

Abstract

A study was conducted to determine the effects of Sauropus androgynus leaf extract on growth, carcass quality and the number of fecal microorganisms in broiler chickens. Forty-eight male Arbor Acres broiler chickens (21-d-old) obtained from a commercial hatchery were used in the present study. Experiment consisted of four treatment groups with four pen replicates of three broilers allotted randomly to each dietary treatment from day 21-42 old. One group was the control with no additional Sauropus androgynus leaf extract (SAE) (P0), and other three groups were given drinking water supplemented with 1.5 g (PI), 3.0 g (P2) or 4.5 g SAE/l water (P3). The diet used was a commercial mix (Crude protein: 19% and Metabolizable Energy 3200 kcal/kg). Feed intake significantly reduced in P2 or P3 as compared with the control (P<0.05). A decrease in feed conversion ratio was observed in treatment groups as compared with Po (P<0.05). Abdominal fat, neck fat and liver fat content were significantly reduced by SAE (P<0.05), while carcass fat content was not significantly different. Number of fecal Escherichia coli in P1 or P3 (P<0,01) and fecal Streptococcus sp and Salmonella sp. were significantly (P<0,01) reduced by SAE supplementation as compared with the control, while fecal Bacillus subtilis in P2 and Lactobacillus sp. in P1 (P<0,01) were significantly higher as compared with other groups. SAE (P2 or P3) also significantly improved meat taste, shank color but lowered meat color (P<0.05) as compared with the control group. It was found that the number of fecal Streptococcus sp was suitable to predict abdominal fat. In conclusion, the inclusion of SAE at 4.5 g/l drinking water resulted in the best performance and carcass quality.   Key words: Sauropus androgynus leaf extract, fat accumulation, broilers
Acid base status in swamp buffaloes (Bubalus Bubalis) fed rice straw and concentrate with addition of sodium Joseph, G
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.511 KB) | DOI: 10.14334/jitv.v6i4.249

Abstract

An experiment was conducted to study the addition of NaHCO3 and Na2CO3 on acid-base status in swamp buffaloes, Three fistulated buffaloes were each introduced to dietary treatment control diett (50% rice straw + 50% concentrate), control + �% NaHCO3 and diet control + 10% Na2CO3 in two times Latin Square Design. The diets contained 9,7% crude protein and 53% TDN to achieve maintenance requirements of the animals. Parameters measured include (l) Fed consumption, water consumption and urine volume. (2) pH in rumen fluid, saliva, bLood and urine, (3) natrium mineral content in rumen fluid, saliva, blood and urine. The results of the experiment showed higher pH in the rumen fluid, saliva, blood and urine of buffaloes due to supplementation of NaHCO3 and Na2CO3, Water consumption and urine volume was significanly increased as the effect of Na supplement. The acid-base status of buffaloes was apparently normal in all animals.   Key words: Acid-base status, rice straw, concentrate, buffer, swamp buffaloes
Polymerase chain reaction optimization for the detection of Pasteurella multocida B:2, the causative agent of Haemorrhagic septicaemia Natalia, Lily; Priadi, Adin
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.103 KB) | DOI: 10.14334/jitv.v6i4.266

Abstract

Specific detection of Pasteurella multocida type B:2 by polymerase chain reaction (PCR), using a set of DNA primers wasoptimised. Effects of the addition of ethylene diamine tetra acetic acid (EDTA) to the sample preparation, Escherichia coli contamination and the number of P. multocida on the PCR product was assessed. The PCR test was compared to the standard bacteriological method for the detection of P. multocida B:2 in tonsillar swab samples collected from slaughter houses of various regions in Indonesia. Addition of 100 mM EDTA-saline to P. multocida B:2 spiked tonsillar swab samples inhibits the production 350 base pairs (bp) PCR product. The inhibitory effect of the EDT A can be eliminated by three times washing with deionised water. The PCR can detect P. multocida as low as I organism and contanimation of 100 CFU of E. coli does not effect the PCR result. The results show that the DNA primers for P. multocida B:2 is sensitive and specific. The inhibitory effect of EDTA in PCR samples can be eliminated by washings. Keywords: PCR, EDT A, Pasteurella multocida B:2
Spontaneous cholangiohepatitis in broiler chickens: immunohistochemical study of Ito cells Handharyani, E; Ochiai, K; Winarsih, W; Harlina, E; Estuninngsih, Salfina Nurdin
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.803 KB) | DOI: 10.14334/jitv.v6i4.245

Abstract

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis andmalformation of extrahepatic biliary tracts.   Key words : Cholangiohepatitis, immunohistochemistry, ito cell, broiler chicken
Non-linear growth analysis of Sumatera thin tail sheep and its cross breds Suparyanto, Agus; ., Subandriyo; Widarya, T.R; Martojo, H.H
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.472 KB) | DOI: 10.14334/jitv.v6i4.250

Abstract

Growth curve is a figure of individual ability to express its genetic potential to maximum size under the existingenvironmental condition. Three non-linear growth curves, von Bertalanffy, Logistic and Gompertz, were used to analyze the weight-age relationship for five genotypes of sheep. The data were collected from IP2TP Sei Putih, North Sumatera. Num ber of animals which were collected consisted of five genotypes i.e, indigenous Sumatera (n=275), St. Croix (n=571), St. Croix Cross (n=899), Barbados Blackbelly Cross (n=471) and composite (n=740). The three non-linear growth curves were compared to obtain the most suitable curve for describing the shape of growth curves among sheep genotypes. The growth curves of von Bertalanffy fitted better than the others. The results showed that regression parameters of B or M (integral constante) were significantly different (P<0.001) among growth curves. It means that higher asymptotic weights will be followed by faster growth rates to reach mature size. Value of A (asymptotic mature weigh) and k (growth rate to mature size) were not significant (P>0.05). The data show that there was correlation between A and k.   Key words: Sumatera thin-tail sheep, crossing, non-linear growth
Nutritive value of palm oil sludge fermented with Aspergillus niger after stored in different packing materials Pasaribu, T; Purwadaria, T; Sinurat, A.P; Rosida, J; Saputra, D.O.D
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.064 KB) | DOI: 10.14334/jitv.v6i4.246

Abstract

An experiment has been conducted to determine the effect of type of packaging and time of storage on fermented palm oil sludge. The palm oil sludge was fermented with Aspergillus niger, dried, kept in differentpackaging materials (plastic bags, feed bag, and paper bag) and stored under room temperatures for 12 weeks. The experiment was assigned in a split plot design (3x7). The parameters measured were water content, crude protein, soluble nitrogen, true protein, in vitro dry matter (IVDMD) and true protein digestibilities (IVTPD), total in vitro digestible protein, and the activities of mannanase and cellulase. Results showed that fermented palm oil sludge stored for 12 weeks increased the water content, decreased the true protein and fiber contents, and also reduced the activity of mannanase and cellulase and in vitro dry matter digestibility but no changes on the crude protein content, protein digestibility, and total digestible protein. It was concluded that fermented palm oil sludge should be kept in feed bag under room temperature if to be stored for 12 weeks.   Key words : Palm oil sludge, fermentation, nutritive value, storage
Antibody fluctuations of infected cattle with Fasciola gigantica and the effect of triclabendazole treatment Widjajanti, S; Estuningsih, S.E; ., Suharyanta
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.383 KB) | DOI: 10.14334/jitv.v6i4.251

Abstract

Observation on the antibody fluctuations of infected cattle with metacercariae of Fasciola gigantica and the effect of triclabendazole treatment were made by means of ELISA technique. Seven cattle were infected with 700 metacercariae and one cattle remained uninfected, as negative control animal. Treatment with triclabendazole was given to 6 cattle, when the mean antibody levels of infected cattle reached the peak, and the other one remained untreated, as positive control animal. One week after treatment the mean antibody levels started to drop and then decreased gradually. After eight weeks of treatment, the mean antibody levels of the treated cattle reached the lowest level or the same value as before infection, thereafter, 6 cattle were reinfected with different dosages of metacercariae of F. gigantica. Two cattle were infected with 400 metacercariae, the other two were infected with 600 metacercariae and the rest of them were infected with 800 metacercariae. The results showed that the immunological responses of re-infected cattle are quicker (5 weeks after infection) and the peak of the antibody levels are higher (ELISA OD = 1.7) than after the first infection (11 weeks after infection and ELISA OD = 1.2). However, after re-infection, there were no significant different on the antibody fluctuations and antibody levels among the infected group, although those cattle received different dosages.   Key words: Antibody, cattle, Fasciola gigantica, ELI
The detection of infectious bronchitis viral antigen by means of immunohistochemical technique in broiler chicken infected with I-269 IB isolate or injected with H-120 live vaccine Damayanti, Rini; ., Darminto
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.179 KB) | DOI: 10.14334/jitv.v6i4.247

Abstract

A study was carried out to detect the antigen of infectious bronchitis vius (IBV) in broiler chicken by means of immunohistochemical technique. A total of 150 - fourteen days old broiler chicken were divided into three groups i.e. 50 chicken were infected with an IB isolate of I-269, 50 chicken were injected with H-120 life vaccine, and 50 chicken served as un-treated control. Clinical signs and gross pathological changes were observed. Each of five chicken of each group were necropsied at 1, 2, 3, 4, 7, 10, 14, 21, 28, and 35 day(s) post infection/vaccination. The antigen could be detected at one day through 35 days post vaccination/infection. In the vaccinated group, histopathological lesions and the detected antigen were minimal. In contrast, the infected chicken showed varied histolopathological lesion in accordance with the numerous antigens. The antigen were observed in the lymphocytes/macrophages in the trachea, lungs and kidney, and in the epithelium of trachea, alveoli, broncheolus and tubular sitoplasm of the kidney of both vaccinated and infected groups. In the infected group, antigen was also detected in the lymphocytes and macrophages of the affected organs.   Key words: Infectious bronchitis, broiler chicken, I-269 IB isolate, immunohistochemistry
Utilization of palm oil sludge in poultry diet. 4. Inclusion of fresh or dried fermented palm oil sludge in native chickens diet Sinurat, A.P; Purwadaria, T; Pasaribu, T; Darma, J; Bintang, I.A.K; Togatorop, M.H
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.316 KB) | DOI: 10.14334/jitv.v6i4.243

Abstract

Fermentation processes increase the protein of palm oil sludge and produce some useful enzymes. However, drying process, especially with heating often affects the nutritive values of feed ingredients and the enzymes activity. Therefore, this experiment was designed to study the responses of native chickens when fed ration containing fresh or dried fermented palm oil sludge (FPOS). Experimental diets with different levels of fresh or dried FPOS (5, 10 and 15% equal to dried FPOS) were formulated with similar nutrient contents. A control diet with no FPOS was also included. Each treatment was replicated 5 times for 12 weeks. Performances (body weight, feed consumption, feed conversion and mortality) of chickens were recorded. Carcass yield, abdominal fat content, weight of liver and gizzard were measured at the end of feeding trial. Data were subjected to analysis of variance and different between means were tested by orthogonal procedures. Results during the starting period showed that, chickens fed FPOS diet gain more weight significantly than the control birds (298.1 vs. 264.7). The dry matter intake of the dry FPOS diet was significantly better than the fresh FPOS diet (2.88 vs. 3.32). The FPOS dietary levels did not affect body weight gain and dry matter intake of native chickens, significantly. However, increasing the FPOS levels worsen the dry matter conversion (DCR) significantly. Data during 12 weeks trial showed mat the body weight gain was not significantly affected by treatments. The dry matter intake of the FPOS diets were significantly higher than the control diet (3469 vs. 3065 g/bird), hence the DCR of the control diet was significantly better than the FPOS diets (3.28 vs. 3.62). Feeding dry FPOS resulted in a better DCR as compared to fresh FPOS (3.48 vs. 3.76), but not affected the dry matter intake. The FPOS dietary levels did not affect body weight gain and dry matter intake of native chickens, significantly. However, inclusion of 5% FPOS in me diet gave better DCR significantly, as compared with 15% FPOS (3.51 vs. 3.83). Feeding dry or fresh FPOS (5 - 15%) did not significantly affect the mortality, carcass yield, abdominal fat levels, weight of liver and weight of gizzard of native chickens. It is concluded that dry FPOS was better than the fresh FPOS and could be included in me native chickens diet up to 10%.   Key words: Palm oil sludge, fermentation, native chickens

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