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Ahmadi Riyanto
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INDONESIA
Indonesian Journal of Animal and Veterinary Sciences
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
JITV (Indonesian Journal of Animal and Veterinary Science),  ISSN: 0853-7380 E-ISSN: 2252-696X is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of animal and veterinary science. It was first published in 1995. The journal has been registered in the CrossRef system with Digital Object Identifier (DOI) prefix 10.14334.
Arjuna Subject : -
Articles 6 Documents
Search results for , issue " Vol 24, No 1 (2019): MARCH 2019" : 6 Documents clear
Phenotypic characteristics of Exotic-broiler, Kampung, Male Exotic-layer, KUB-1 and Pelung chickens Saragih, Hendry Tri; Viniwidihastuti, F.; Lembayu, R. P.; Kinanthi, A. R.; Kurnianto, H.; Lesmana, I.
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 1 (2019): MARCH 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i1.1889

Abstract

The needs for chicken meat have been dominated by meat from exotic broilers (bred from imported parent). The potential of local broilers chicken in Indonesia is expected to be able to provide the meat for national needs for chicken meat. The objective of this research was to determine the day-old-chick (DOC) phenotypic characteristics of chickens in Indonesia that have the potential as broilers. The phenotypic characteristics are in the form of body morphometry, visceral organ weight and small intestinal histo-morphology. The chickens used were the DOC type of exotic Broiler, Kampung, exotic male Layer, KUB-1 and Pelung. A total of 125 chickens consisting of 25 chickens of each strain at posthatched age were observed for its body morphometry and its visceral organ weight, and small intestinal histo-morphology. The observed data were then analyzed by similarity analysis using MVSP 3.22 to create a dendogram with the Unweighted Pair Group with Aritmetic Average (UPGMA) method. Results showed that there were 2 different clusters from the level of similarity in their characteristics. Exotic broilers had 94.65% similarity to male exotic layer chicken and 92.26% to pelung chicken, while Kampung chickens had 90.16% similarity to KUB-1Chicken. In conclusion, it is indicated that the level of similarity of the phenotypic characteristics of pelung chickens were close to the type of exotic broiler and male exotic layer chicken. This level of similarity lead  to the expectation that pelung chickens potential to be a candidate for meat-type of local chicken.
The effect of addition selected amino acids in extender semen on quality and DNA stability of frozen-thawed Sumba Ongole bull spermatozoa Said, Syahruddin; Setiorini, Setiorini; Adella, Amaitshaa; Sari, Indah; Fathaniah, Nursafira; Maulana, Tulus
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 1 (2019): MARCH 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i1.1873

Abstract

The objective of the current study was to asses the optimal concentration of glutamine, glycine and cysteine amino acids in tris-citric-acid-fructose egg yolks (TCFY) extender on quality of SO bull spermatozoa during freezing and thawing. In this study the DNA stability of frozen-thawed Sperm was also indentified. Three mature bulls maintained at PT. Karya Anugerah Rumpin, private cattle breeding company, West Java, Indonesia were used as semen donors. Semen was collected using artificial vagina and were evaluated prior to freezing. Semen was diluted with TCFY supplemented with different concentrations of amino acids (5, 15 and 25 mM glycine and glutamine, and 3, 5 and 7 mM cysteine) then processed for colling and freezing. Semen quality parameters (subjective motility, viability and membrane and DNA integrity). Data showed that in general the effect of addition of selected amino acids (glycine, glutamine and cysteine) into TCFY extenders on motility, viability and membrane integrity of SO spermatozoa after cooling were significantly different (p<0.05) higher than that of control. Addition of 15 mM glycine, 15 mM glutamine and 5 mM cysteine resulted in significant (p<0.05) increase post-thawing sperm motility and sperm viability as compared to that of control. Furthermore, when spermatozoa were stained with acridine orange after fixation with acetic alcohol, the DNA integrity of post-thawing spermatozoa showed that all spermatozoa were remain intact. In conclusion ,addition of 15 mM glycine, glutamine and 5 mM cysteine increase the cryoprotecting efficacy of bovine bull cryopreservation extender, and furthermore all DNA spermatozoa were remain intact. 
The relationship of pod colour with the quality of Indigofera zollingeriana Hutasoit, Rijanto; Riyadi, .; Sirait, Juniar
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 1 (2019): MARCH 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i1.1923

Abstract

Indigofera zollingeriana (Indigofera) plant is potential feed ingredients. The propagation of this plant is through seed. The low quality of seed is a problem in its development. This study was aimed to evaluate the relationship of pod colour with quality of Indigofera seeds. The study was designed in a complete randomized design consisting of four pod colours and four replications, namely: P1= green, P2= brownish green, P3= brown, and P4= black. The parameters observed were: characteristic and morphology of pods and seeds of Indigofera, the growth of sprouts, and the growth of fungus on Indigofera seed. Results showed that the number of pests was fewest found in P2, brownish green pod (14%). The highest number of seeds was in P1, green pod (5173) and P2, brownish green pod (4944). The highest germination (62%) was detected in P2 (brownish green). The heaviest sprout was in P2, in brownish green pod (0.035g), highest sprout (2.68 cm) in P4, black pod colour. Based on fungus observation, the black pod (P4) provided the fewest result (6.63%), however most fungus grew very well in P1, the green pod (47.88%). It could be concluded that the brownish green pod colour was the best phase for harvesting good quality I. zolligeriana seed.
Generation of scFv-Monoclonal Antibody Avian Influenza Diagnostic tests Tarigan, Simson; Sumarningsih, .
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 1 (2019): MARCH 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i1.1871

Abstract

The need for rapid diagnostic tools or point- of- care diagnostic tests for Avian Influenza in Indonesia is very high and the price of these imported diagnostic tools is very expensive. As a result, a large budget requires to provide the needs. The main component of a rapid diagnostic tool is the monoclonal antibody (mAb) specifically recognized influenza viruses. The objective of this study was to produce mAb that can recognize all subtypes of Avian Influenza viruses using the phage display technology. Influenza-A focused scFv commercial library was panned using alternating recombinant H1N1 NP and H5N1 virions. Whereas, bacteriophages bound to the panning baits were eluted with serum from H5N1-infected chickens. Phagemid from suppressor E. coli (TG1) infected with bacteriophage displaying anti-NP on its surface was isolated and then transformed into a non-suppressor E. coli (HB2151) to express NP-scFv. Monoclonal NP-scFv antibody with a molecular weight of about 27 kDa was purified from the culture supernatant using a nickel-chromatography column. The amount of pure NP-scFv obtained was around 1.2 mg /L culture. As an additional component for its use in immunoassays, antibody to NP-scFv was produced in rabbits. The generating polyclonal antibody recognized the NP-scFv specifically and sensitively. The anti-NP-scFv monoclonal antibody and the anti rabbit scFv polyclonal antibody produced in this study are envisaged appropriate for the development of diagnostic tools for point-of-care for Avian Influenza.
The influence of dietary protein and energy levels on the performance, meat bone ratio and meat chemical composition of SenSi-1 Agrinak chicken Hidayat, Cecep; Iskandar, Sofjan
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 1 (2019): MARCH 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i1.1913

Abstract

Sensi-1 Agrinak is a strain of the improved native chickens for meat production in Indonesia. The objective of this study  was to investigate influence of different dietary energy and protein levels on performance, meat bone ratio, and meat chemical composition of Sensi-1 Agrinak chicken, reared until 10 weeks of age. Two hundred and sixteen of unsexed day old chickens (DOC) of  Sensi-1 Agrinak were subjected to six experimental rations differed in dietary crude protein (CP) content,. Namely: 21;19; and 17 % and dietary  metabolizable  energy (ME) (2800 and 3000 kcal/kg). Each treatment combination was replicated  four times and fed from day old to 10 weeks old. In each treatment combination there were nine unsexed-DOCs. The parameters observed were performance (i.e. live weight, feed intake, viability, FCR), economic index (European Production Efficiency Factor/EPEF), meat bone ratio, and meat chemical composition.  Result showed that  increased of dietary CP level increased live weight and EPEF (p<0.05). Sensi-1 Agrinak chicken had the best FCR (2.59), when fed  diet containing 21% CP and 3000 kcal/kg. Increased dietary CP level increased the  meat-bone ratio of breast, thighs, and drumsticks. Meanwhile, increased levels of dietary CP and ME did not affect (p>0.05) meat chemical composition. It is concluded that optimal dietary levels of crude protein and energy for unsexed Sensi-1 Agrinak chicken  up to 10 weeks of age were 21% CP and 3000 kcal/kg.
Evaluation of surra treatment strategies attacking horses and buffaloes in East Sumba District, Nusa Tenggara Timur Province of Indonesia (2010 – 2016) Dewi, Rita S.; Wardhana, April H.; Soejoedono, Retno D.; Mulatsih, Sri
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 1 (2019): MARCH 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v24i1.1864

Abstract

Surra is a disease attacking livestock caused by a flagellated protozoan, Trypanosoma evansi. Indonesia archipelago is reported as an endemic country of the disease, except Sumba Island.  However, Surra outbreak occurred in this Island in 2010 due to livestock movement from the neighbour island, Sumbawa. It generated high mortality in livestock, particularly in horses and buffaloes. The aim of the study was to evaluate the effectiveness of Surra treatment strategies in East Sumba District from 2010-2016 and to estimate the incidence of Surra in the next few months (forecast). The treatment strategy of Surra in East Sumba was divided into 2 (two) periods e.g. the first period in 2010-2011 using Isomethamedium as the single drug (period I) and the second period in 2012 - 2016 using a combination between diminazene aceturate as curative and isomethamedium as a prophylactic drug (period II). All data in the present study was obtained from the local livestock agency of East Sumba District from 2010 – 2016 when Surra outbreak occurred. The effectiveness of those two treatment strategies was compared using the proportion test. The results demonstrated that morbidity and mortality of horses and buffaloes were significantly greater in the period I (2010-2011) compared to period II (2012-2016). The treatment strategy in the period II was able to decrease the proportion of morbidity in horses and buffaloes for 1.44% and 0.66%, respectively.  Likewise, the proportion of mortality in period II was also less than the period I from 3.79% to 1.30% for horses and from 2.80% to 0.55% for buffaloes. Based on forecasting study analysis using the control program projected with decomposition method for the next 12 months demonstrated that the treatment strategy in the period II could reduce the incidence and death of livestock by Surra. The treatment strategy using a combination between isometamedium and diminazene aceturate in East Sumba District might be more effective compared to using isometamedium alone. 

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