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Ahmadi Riyanto
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INDONESIA
Indonesian Journal of Animal and Veterinary Sciences
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
JITV (Indonesian Journal of Animal and Veterinary Science),  ISSN: 0853-7380 E-ISSN: 2252-696X is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of animal and veterinary science. It was first published in 1995. The journal has been registered in the CrossRef system with Digital Object Identifier (DOI) prefix 10.14334.
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Articles 10 Documents
Search results for , issue " Vol 19, No 1 (2014): MARCH 2014" : 10 Documents clear
Formation of germline chimera Gaok chicken used circulation primordial germ cells (circulation PGCs) fresh and thawed T, Kostaman; TL, Yusuf; M, Fahrudin; MA, Setiadi; AR, Setioko
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.275 KB) | DOI: 10.14334/jitv.v19i1.990

Abstract

Formation of germline chimeras by transfer of chicken primordial germ cells (PGCs) is one of the effective techniques for preservation and regeneration of genetic resources in chickens. This study attempted to form germline chimeras of Gaok chicken buy purifying circulated PGCs of donor embryo before it is transferred to the recipient (White Leghorn chickens=WL) and studied the ability of recipient embryo on survival in incubators, and hatchability. This study used 200 fertile eggs of Gaok and 90 fertile WL breed all of the eggs was incubated at 380C and 60% humidity in a portable incubator. PGCs-circulation of the blood collected Gaok embryos at stage 14-16 were taken from the dorsal aorta, and then purified by centrifugation method using nycodenz. PGCs-circulation results further purification frozen in liquid nitrogen before being transferred to the recipient embryo. The results showed that for the development of embryos transferred to the fresh circulation of PGCs-circulation as many as 25 cells can survive up to day 14, while one of the transferred of 50 and 100 cells into recipient embryos was hatched (10%). On the contrari recipient embryos that are transferred to the frozen PGCs-circulation the embryos development was shorter, and only survived until day 10th (treatment 25 cells), day 14th (treatment of 50 cells) and day 17th (treatment of 100 cells). It is concluded that the amount of PGCs-circulation embryos transferred to the recipient is one factor that influence the success of the development germline chimeras. Key Words: Gaok Chicken, White Leghorn Chicken, Circulated PGCs, Transfer, Germline Chimera
Efficacy of application of vaccine AI H5N1 clade 2.1.3 on Mojosari ducks challenge against AI H5N1 clade 2.3.2 in laboratory conditions R, Indriani; NLPI, Dharmayanti; R.M.A., Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.093 KB) | DOI: 10.14334/jitv.v19i1.995

Abstract

Influenza virus HPAI H5N1 clade 2.3.2 outbreaks since September 2012 caused high mortality in ducks. Vaccination is one of strategies recommended by government. However, AI H5N1 clade 2.3.2 vaccine not yet available during this research, while AI H5N1 clade 2.1.3 vaccines available in markets. Therefore it was important to do study on efficay of HPAI H5N1 clade 2.1.3. vaccines on duck at laboratory scale. Three groups of Mojosari duck were used in this study, they were 1 group vaccinated with A Vaccine, 1 group vaccinated with B Vaccine, and 1 group as control (not vaccinated). Vacination groups consisted of 9 DOD and control group was consisted of 6 DOD. Vaccination was conducted when the duck at three weeks old of age using single dose recommended by producer. At three weeks later (ducks at 6 weeks old of age) all Groups of ducks were challenged with virus HPAI H5N1 clade 2.3.2 at dose 106 EID50/ml by drops intranasaly. Result showed that Group 1 (vaccinated with A Vaccine) produced 67% protection (3 out of 9 ducks died), Group 2 (vaccinated with B Vaccine) produced 100% protection (non out of 9 ducks died), and Group 3 (control, not vaccinated) produce 0% protection (all of 9 ducks died). This study give an alternative of choise to use AI H5N1 Clade 2.1.3 vaccine with high protection when AI H5N1 Clade 2.3.2 vaccine not available in markets to controll high mortality in ducks caused by HPAI H5N1 clade 2.3.2 outbreaks. Key Words: Duck, HPAI, AI, Avian Influenza, Vaccine
Carcass profile and chemical characteristic of male Kacang goat (Capra aegragus hircus) I, Mirdhayati; J, Hermanianto; CH, Wijaya; D, Sajuthi
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.143 KB) | DOI: 10.14334/jitv.v19i1.991

Abstract

The purpose of this research was to characterize the carcass profile and chemical characteristic of male Kacang goat (local name for Capra aegragus hircus) from two different slaughter ages, namely < 1.5 and > 1.5 years old. Kacang goats generally is slaughtered for consumption at these ages. The body part used for chemical analysis was the carcass leg. Data was analyzed statistically by t test. Results showed that there was no profile differences of the carcass from both group ages obtained from similar rearing system and at common range of body weight for slaughtering. Chemical characteristics measured by the contents of protein, fat, moisture, ash and cholesterol level in goat meat showed no differences between the two slaughter ages. Dominant amino acids found in the goat meat was glutamic acid, aspartic acid, leucyne and lysine. Saturated and unsaturated fatty acid profiles in the goat meat did not show any different between the two slaughter ages, except for eicosatrienoic acid, in which the meat from the younger goat had significantly higher content of eicosatrienoic acid. The meat of male Kacang goat of both groups is a potential source of healthy meat, based on its low fat content, functional amino acid composition, higher desirable fatty acids and fatty acid ratio of omega 6 : omega 3 which corresponds to the values recommended by American Heart Association 2008. Key Words: Male Kacang Goat (Capra aegragus hircus), Carcass, Cholesterol, Amino Acids, Fatty Acids
Efficacy of micro-encapsulated of local isolate B. thuringiensis as bio-insectiside for control of myiasis caused by Chrysomya bezziana larvae S, Muharsini; AH, Wardhana
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.209 KB) | DOI: 10.14334/jitv.v19i1.996

Abstract

B. thuringiensis produces toxic crystal proteine (-endotoksin) which is specific for insect target, but non-toxic to human or non-target organism. Local isolate of B. thuringiensis have been collected from Kediri Regency where endemic area of myiasis. The aim of this study was to formulate the micro-encapsulated of B. thuringiensis using Freund Incomplete Adjuvant (FIA) for protecting crystal in the field and enviromentally friendly. The formulae was then tested in vitro and in vivo. The result of in vitro trial towards seven local isolates of B. thuringiensis showed a decreased in the toxicity of those several isolates. In vivo trial of nine thin tail sheep using chosen isolat of 45.5A and 47.3A, resulted is no significant different for treatment sheep compared to control sheep (P > 0.05). It is concluded that micro-encapsulation method need to be developed according to the way of living the C. bezziana larvae in animal tissues. Key Words: Chrysomya bezziana, Bacillus thuringiensis, Freund Incomplete Adjuvant, Micro-encapsulated
Genetic polymorphism of the β-lactoglobuline gene in Friesian Holstein cows HS, Nury; A, Anggraeni
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.997 KB) | DOI: 10.14334/jitv.v19i1.992

Abstract

Milk protein in dairy cattle consists of casein and whey. Whey in milk of dairy cattle is about 20% with the main component is β-lactoglobulin (7-12%). Polymorphism of the β-lactoglobulin gene affects protein and whey production in milk. Selection at a molecular base to improve whey and protein in milk requires information on genetic diversity of the β-lactoglobulin gene, besides other protein genes. This study was aimed to identify genetic polymorphism of the β-lactoglobulin gene in 88 heads of Friesian Holstein (FH) cows at Cikole Dairy Cattle Breeding and Improvement Station (Cikole DCBIS), Lembang. Genotyping was done using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method, and a HaeIII restriction enzyme. Genotype frequency, allele frequency, and heterozygosity value were calculated by Nei method. Hardy-Weinberg equilibrium was calculated by chi-square test. Result showed that the β-lactoglobulin gene in the observed HF cattle was polymorphic, in which there were two alleles (A and B) and three genotypes (AA, AB and BB). Frequencies of the A and B alleles were 0.40 and 0.60 respectively; while those of the AA, AB, and BB genotypes were succesively 0.10; 0.60 and 0.30. Heterozygosity value was obtained for 0.483. The β-lactoglobulin gene was in Hardy - Weinberg equilibrium (χ2cal < χ2table). It is concluded that polymorphic of the β-lactoglobulin gene can be used as an initial information for molecular selection on milk protein composition in FH cows. Key Words: HF Cattle, β-lactoglobuline Gene, Genetic Polymorphism, PCR-RFLP
Detection of chloramphenicol residue in bovine meat using Liquid Chromatography Mass Spectrometry R, Widiastuti; Y, Anastasia
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.88 KB) | DOI: 10.14334/jitv.v19i1.997

Abstract

Chloramphenicol (CAP) is a broad spectrum antibiotic that has been banned in many countries due to its serius side effect to human. Detection of CAP residue in food has been determined to a minimum required performance limit (MRPL) of 0.3 ng/g. The purpose of this research was to conduct the analysis of CAP residue in bovine meat by using LCMS and to study the presence of CAP residue in marketed bovine meat samples. LC separation was done on a Shimpack column C18 with ammonium acetate 10 mM/water as mobile phase, and ESI-MS analysis in negative ion mode. The coefficient of determination, R2 = 0.9981 at concentration of 0.125, 0.25, 0.63, 1,00 and 2.00 ng/g. Recovery at three fortification levels (0.25, 0.50 and 1.00 ng/g) was in the range 77.5, 97.3 and 83.4%. The decision limit and the detection capability were 0.15 ng/g and 0.17 ng/g respectively. Analysis results of 52 marketed samples showed that CAP residue were detected in 9 samples in the concentration range of 0.14 to 2.70 ng/g and 6 among those positive samples were above the MRPL value. Therefore, it is important to increase the awareness and also to monitor regularly CAP residues in food originated from animal to provide safe food for the consumers. Key Words: Chloramphenicol, Residue, LCMS, Bovine Meat
Morphological characters of Panicum maximum cv Gatton planted from different source of sapling A., Fanindi; E, Sutedi
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.541 KB) | DOI: 10.14334/jitv.v19i1.988

Abstract

Morphological characters and agronomic information of Panicum maximum cv Gatton is required in forage crop breeding activities. Therefore a research was conducted to determine the morphology and characteristic of generative phase of Panicum maximum cv Gatton. The study was conducted in greenhouses, using a 40 cm diameter pot. The planting medium was soil from Ciawi (Latosol). Research design was Completely Randomized Design, with 10 replications. Treatments used were 2 types of planting material, 1) derived from the pols (vegetative) and 2 seeds. Plants used were Panicum maximum cv Gatton that have been collected and evaluated at the IRIAP, Ciawi. Variables measured were plant morphology, reproductive traits, seed production per panicle and seed quality. Results showes that morphological characters of cv Gatton derived from seed and pols was not significantly different (p > 0.05), whereas the flowering time and the time of seeds mature after cutting derived from the seeds was faster (p < 0.05) than cultivars derived from pols. While the production of seed derived from the seeds was higher (p < 0.05) when compared to that derived from the pols. Key Words: Morphological Characteristics, Seed, Pols, Panicum maximum
Biolarvacidal activity of methanol extract of Kipahit leaves (Tithonia diversifolia) against larvae of Chrysomya bezziana fly AH, Wardhana; N, Diana
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.467 KB) | DOI: 10.14334/jitv.v19i1.993

Abstract

Myiasis or “belatungan” is the infestation of live human and vertebrate animal tissue with dipterous larvae, Chrysomya bezziana. In general, synthetic insecticides were applied to control the disease. However, it causes negative impact on animal product, so that it is required to find an alternative treatment using herbal medicine. The aim of this study was to access activity of methanol extract of Kipahit leaves (Tithonia diversifolia) against various stages of C. bezziana larvae (L1, L2 and L3). Five treatments were applied with five replications, i.e. control/water (P0), 0,5%, 1%, 2% of the extract for PI, PII and PIII, respectively. Another treatment was 0,05% Asuntol for positive control (PIV). Each treatment was added with1% DMSO and twenty larvae were examined for each replication. Bioassay of L1 and L2 was addressed to investigate effect of intestinal toxicity by mixing the extract with Meat-Blood Mixture (MBM) and Larval Rearing Media (LRM) for L1 and L2, respectively. Bioassay of L3 was to investigate effect of contact toxicity through soaking the larvae into the extract solution for 10 seconds followed by incubating in vermiculite at 36oC. All living larvae after being treated by various concentration of the extract were reared to pupae and allowed to emerge as imago. Number of living larvae and pupae, pupae weight and number of imago were observed. All data were analysed using ANOVA followed by Dunnett test at 95% confidential limit. For L2, larval mortality were counted and probit analysed using POLO-PC software, therefore the lethal concentration (LC50 and LC95) and lethal time (LT50 and LT95) were defined. Results demonstrated that 1% of the extract was the most effective concentration which was able to kill the larvae and decrease the pupae weight. It also caused to fail pupation and imago emergence. The further study might be pursued to investigate in vivo assay of the extract in infested livestock. Key Words: Tithonia diversifolia, Myiasis, Chrysomya bezziana, Biolarvasidal
Effect of sun flower oil addition (Helianthus annuus) in diet on nutrient intake, growth performance and characteristics of estrous of pre-mating Garut sheep L, Khotijah; R, Zulihar; MA, Setiadi; KG, Wiryawan; DA, Astuti
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.873 KB) | DOI: 10.14334/jitv.v19i1.989

Abstract

Nutritional status of ewes at pre-breeding phase is critical for good reproductive process. Thirty-two ewes Garut sheep (initial body weight 22.5±2.21 kg) were used in a completely randomized design to evaluate the effects of addition of sunflower oil in the diet on nutrient intake, growth performance and characteristics of estrous of pre-mating garut sheep. Ewes were fed grass and supplemented with one of four isonitrogenous high concentrate diets containing four levels of sunflower oil; they were (M0) no addition of sunflower oil; (M1) 2% sunflower oil; (M2) 4% sunflower oil and (M3) 6% sunflower oil addition. Results showed that the addition significantly reduced dry matter intake (p < 0.05), highly significantly reduce crude protein, Ca and P intake (p < 0.01), higly significant increased ether extract intake (p < 0.01) and significantly increased long of estrous (p < 0.05). There was no difference in crude fiber intake, TDN intake, body weight gain, length of estrous onset and response of estrous for ewes fed the four experimental diets. Response on estrous of treatments M0, M1, M2 and M3 were 62.50; 50.00; 75,00 and 62.5% respectively. It is concluded that the addition of sunflower oil up to 6% in the pre-mating ration affects the nutrient intake without interferering the performance, and it tends to improve the charachteristic estrous of Garut ewes. Key Words: Nutrient Intake , Estrous, Garut Sheep, Performance, Sunflower Oil
Antigen detection of rabies virus in brain smear using direct Rapid Immunohistochemistry Test R, Damayanti; I, Rahmadani; Y, Fitria
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.552 KB) | DOI: 10.14334/jitv.v19i1.994

Abstract

Rabies is zoonotic disease caused by a fatal, neurotropic virus. Rabies virus is classified into the Genus of Lyssavirus under the yang family of Rhabdoviridae. Rabies affecting hot- blooded animals, as well as human. Dogs, cats, monkeys are the vectors or reservoirs for rabies and the virus was transmitted through the saliva after infected animal’s bites. The aim of this study was to conduct rapid diagnosis to detect rabies viral antigen in brain smear using immunohistochemical (IHC) method namely direct Rapid Immunohistochemical Test (dRIT). A total number of 119 brain samples were achieved from Bukittinggi Veterinary Laboratory, West Sumatra. Standardisation and validation of the method were compared to Fluorescent Antibody Test (FAT) as a golden standard for rabies diagnosis. Results show that dRIT was a very good method, it can be performed within two hours without the need of fluorescent microscope. The samples were tested using FAT and from 119 samples tested, 80 (67.23%) samples were positive for rabies and 39 (32.77%) samples were negative for rabies whereas using dRIT showed that 78 (65.54%) samples were positive for rabies and 41 (34.45%) samples were negative for rabies. The dRIT results were validated by comparing them with FAT results as a golden standard for rabies. The relative sensitivity of dRIT to FAT was 97.5% and the relative specificity to FAT was 100% (with Kappa value of 0.976, stated as excellent). The achievement showed that dRIT is very potential diagnostic tool and is highly recommended to be used widely as a rapid diagnosis tool for rabies. Key Words: Rabies, Antigen Detection, Immunohistochemistry, Direct Rapid Immunohistochemical Test (dRIT)

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