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Indonesian Journal of Animal and Veterinary Sciences
JITV (Indonesian Journal of Animal and Veterinary Science),  ISSN: 0853-7380 E-ISSN: 2252-696X is a peer-reviewed, scientific journal published by Indonesian Center for Animal Research and Development (ICARD). The aim of this journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of animal and veterinary science. It was first published in 1995. The journal has been registered in the CrossRef system with Digital Object Identifier (DOI) prefix 10.14334.
Articles
1565
Articles
Hematologic Representation of the White Rat Post-implantation Stainless Steel 316L Local Products on Femur Bone

Gustian, . ( Institut Pertanian Bogor )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 3 (2018): SEPTEMBER 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

 This study aimed to obtain information regarding complete blood count (CBC) profile of post implantation stainless steel (SS) 316L as a national local product of non-degradable metal implant. Thirty adult male rat, aged approximately 12 weeks were divided into 3 groups, i.e.control group without implantation, implant group with import SS316L and implant group with Indonesian national local SS316L that developed by Agency for the Assessment and Application of Technology (BPPT). The implant groups were given implants by inserting it between femoral bone and biceps femoris muscle. Examination of the systemic response was done with CBC before and 30 days after implantation. The analysis of red blood cells amount, haemoglobin level, haematocrite value, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell and its differentiation from each group did not show significant differences. In conclusion, stainless steel 316L of import and national local product were inert and showed non-negative effects of blood profile.

The Effectiveness of Combination of Bioactive Substances Some Plants To Inhibit Escherichia coli and Salmonella sp growth on In Vitro and immune response and blood profile on In Vivo

Pasaribu, Tiurma ( Indonesia Research Institute for Animal Production )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 3 (2018): SEPTEMBER 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

The use of antibiotic growth promoters (AGP) has been baned as feed additives in many countries, therefore the alternatives need to be found.  An in vitro experiment  was conducted to study the potential of combination of some plant extract to inhibit growth of pathogen bateria that normally occur in the poultry gastro intestinal tract.The combination of three plants bioactives (liquid smoke of cashew shells of Anacardium occidentaleor CLS, Phyllanthus niruri L. extract (EM), and Synzygium aromaticum extract (EDC) were formulated with and evaluated for its effectiveness to inhibit growth of Escherichia coli and Salmonella sp.invitro.The mixtures (KE) were then made in 3 different concentration of the mixtures, i.e. 100 % KE, 50% KE and 25% KE and studied their effectiveness to inhibit growth of E. coli or Salmonella sp. by using microplate reader method. The results showed that the higher the concentration of the bioactive combination (KE100) the higher the ability to inhibit the growth of E. coli or Salmonella sp. The combination of bioactive substances CAM, EM, and EDC more effectively than Zn-bacitracin antibiotics to inhibit the growth of E. coli and Salmonella sp. The optimum concentration of KE with the similar effectiveness as the AGP was 25%. It was concluded that the combination of CAM, EM, and EDC was able to inhibit the growth of E. coli and even capable to eliminate the presence of Salmonella sp. In the biological assay, a combination of CAM, EM, and EDC either extract or powder form, high dose, medium or low does not affect the weight of the spleen, bursa fabricius, and blood profile. The best combination of CAM, EM, and EDC extracts to decreases the total bacterial population and E. coli is middle dose ie  0.0625% (extract) and 0.625% (powder). Likewise for live weight gain that was a good dose of extract or powder form can replace antibiotics. It was concluded that combinations of CAM, EM, and EDC had potential as a substitute for AGP in poultry feed, especially chickens.

Nutrition Quality and Microbiology of Buffalo, Cow and Goat Milk From West Sumatra

Ferawati, . ( Andalas University )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 3 (2018): SEPTEMBER 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

The aim of this research was to determine the quality of fresh milk physically, chemically and microbiologically obtained from cow, goats and buffalo in West Sumatra. The research method used is laboratory experimental to analyze nutritional value, the number of aerobic bacteria and lactic acid bacteria, isolating and identifying lactic acid bacteria The results showed the nutritional value of milk had meet the requirements of Indonesian National Standardization, but the total colony of aerobic bacteria was above the allowed threshold of 1x106 CFU/ml. In addition, each sample had a total colony of varied lactic acid bacteria. The lowest total BAL value obtained in cows milk is 0,84 ± 0,18 x107 CFU/ml, in contrast to buffalo milk and goat milk which has a higher total BAL of 36,8 ± 17,57 x107 CFU/ ml and 57,25 ± 8,89 x107 CFU/ml. However, all the colonies showed almost identical morphology of BAL isolates. From the results of the study concluded that fresh milk from West Sumatra has the potential to produce BAL although sanitation control is still needed during handling of milk.

Blood Biochemical Components and Progesterone Hormone on Day of Estrus in Crossbred Cattle in Egypt

Mourad, Reem ( Animal Production Department, Faculty of Agriculture, Menufiya University, Egypt )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 3 (2018): SEPTEMBER 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

Deficiencies or excess minerals such as P, Cu, and Zn are associated with subnormal fertility and anoestrus conditions in cows. This study was conducted in a veterinary unit in Menufiya, Egypt.  Eighteen head of crossbred cows were randomly selected at random at estrus time (estrus group) and as a control 14 head of crossbred cows were selected from newly-calving dams at about 6-12 hours after calving (control group). The aim of this study was to use the components of biochemistry and progesterone hormone on estrus day of crossbreeding cattle in Egypt.  The information from this study will be used to confirm estrus time to improve mating percentage. In blood plasma, the concentrations of all biochemical components and progesterone concentrations in estrus animals are higher than control cows except globulin. The results showed that blood plasma from control animal obtained Mg, Ca / P ratio, Co, Cu, Zn, Se, and Mo concentration is higher than estrus cattle. On the other hand, blood plasma concentrations of Na, K, Ca, P, Mn, and Fe are higher in estrus cattle. The progesterone concentration of estrus cattle is lower during summer than in winter. In estrus cows, higher plasma macro-elements were found in winter than in summer except for K, Ca and Ca/P ratio.  On the other side of estrus cattle, all trace elements of blood plasma are higher in winter than in the summer except Zn, Mn, Se, and Fe.

Aflatoxin M1 in fresh dairy milk from small individual farms in Indonesie

Widiastuti, Raphaella ( Indonesian Research Center for Veterinary Sciences (IRCVS) )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 3 (2018): SEPTEMBER 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

This present study was aimed to investigate the presence of aflatoxin M1 (AFM1) residue in fresh dairy milk collected from small individual farms. A total of 104 samples of fresh cows milk were collected from 3 different locations namely Pengalengan-Bandung and Sukabumi (West Java province), and Tanggamus (Lampung province) in April and September 2012. All samples were analyzed using a high performance liquid chromatography (HPLC) and detected with fluorescence detector after extraction with organic solvent. The results showed that high incidence of AFM1 contamination was found in samples from Pangalengan-Bandung and Sukabumi collected in September 2012 with the highest concentration of 34.1 ng/L in a sample from Pangalengan-Bandung, but not found in samples from Lampung for sampling time neither in April and September 2012 Aflatoxin B1 (AFB1) contaminants in low levels were also found in various feed samples. In this study, no sample containing AFM1 exceeded the maximum residue limit (MRL) regulated by the Indonesian government (500 ng/L). Although the results obtained in this study are still not harmful to human health, but the presence of AFM1 in milk and its products should be monitored continuously given the milk is one type of food source that is consumed mainly by children.

Ultrasonography and vaginal cytology diagnostics of the queens

Pertiwi, Amira Putri ( Program Pendidikan Profesi Dokter Hewan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor ) , Tumbelaka, Ligaya ITA ( Departemen Klinik Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor ) , Ulum, Mokhamad Fakhrul ( Departemen Klinik Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 3 (2018): SEPTEMBER 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

Ultrasonography is a diagnostic method to image the conditions of reproduction organs and it could be supported by vaginal cytology to identify the activities of the ovaries by the types of vaginal exfoliate cells. A total of 10 individual queens were used in this study and then grouped into intact group (n=5) and spayed (ovariohysterectomy) group (n=5) based on the anamneses or their medical history. The vagina, cervix, uterus body and horns, and ovaries were imaged and measured by ultrasound. Vagina, uterus body and horn seem as pipe-like structures with hyperechoic outer lines. The lumen in uterus body and horn seem as a hyperechoic structure. The ovaries seem as round- or oval-shaped structures with anechoic follicles. The corpus luteal has thick wall and seen as anechoic in its centre part. The corpus albicans seems as a hyperechoic structure. The vagina of spayed queens seemed more corrugated than the ones of intact queens. In pararelly with ultrasonography, exfoliate vaginal epithelial cell types were then also be identified and counted on each queens. The results of vaginal cytology showed that proestrus occured in 3 intact queens, late metestrus in 1 intact and 3 spayed queens, anestrus in 1 spayed queen, and unidentifiable estrus stage in 1 intact and 1 spayed queens. Moreover, the morphology of cervix and uterus was affected by the activity of ovary.

The Correlation of Electric Conductivity Values with the Dairy Milk Quality

Yanthi, Nova Dilla ( Bogor Agriculture University Pusat Penelitian Bioteknologi LIPI, Cibinong, Bogor ) , Said, Syahruddin ( Pusat Penelitian Bioteknologi LIPI, Cibinong, Bogor ) , Anggraeni, Anneke ( Balai Penelitian Ternak, Ciawi, Bogor ) , Damayanti, Retno ( Fakultas Kedokteran Hewan, Institut Pertanian Bogor ) , ., Muladno ( Fakultas Peternakan, Institut Pertanian Bogor )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 2 (2018): JUNE 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

Milk, as the prime source of food for mammals, has an electrolyte to replace the loss of body fluid caused by activity or metabolism process. The total electrolyte concentration can be measured based on conductivity value from the nutritional content. Therefore, the parameter of the quality of milk with conductivity value can be a benchmark for quality and selling value of milk, making it simpler to be implemented in the field. The aim of this research is to analyze the relation between electric conductivity (EC) with the content value of cow milk. The milk was taken from 10-30 cows from a farm in Lembang (district of West Bandung), Pengalengan (district of Bandung), Tasikmalaya, Sumedang, Subang, Sukabumi and Bogor of West Java Province. The milk was put in 50 ml of sterile falcon. The Probe EC count-meter CT-3031 was used to measure EC while the quality of milk was measured by Probe MilkoScanTMFT 120 (Foss).  The milk quality is reflected by protein content, Fat, Total Solid (TS), Solid Non-fat (SNF), Lactose, Density, Acidity and Freeze Point Deviation (FPD). The results of this study show that the EC value in the milk gives a very real positive effect (p=<0.01) to Total Solid (TS), Solid Non-fat (SNF), Lactose, and Freeze Point Deviation (FPD). The value of EC also significantly affect (p=<0.05) the value of density in milk. Therefore, the value of EC can be used to predict the quality value of milk.

The Effect of Adding Glucomannan Derived from Porang Tuber Extract (Amorphopallus onchophyllus) on Dietary Protein Utilization in Broiler Chicken

., Khanifah ( Mahasiswa S1 Peternakan Fakultas Peternakan dan Pertanian Universitas Diponegoro, Semarang ) , Suthama, N. ( Staf pengajar Fakultas Peternakan dan Pertanian Universitas Diponegoro, Semarang ) , Wahyuni, H. I. ( Staf pengajar Fakultas Peternakan dan Pertanian Universitas Diponegoro, Semarang )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 2 (2018): JUNE 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

The aims of this study was to evaluate the affect of dietary addition of glucomannan derived  from porang (Amorphophallus oncophyllus) tuber extract (GEUP) in broiler  ration on performance of broiler chicken. There  were 160 birds of day old broiler chicks of New Lohmann strain with average initial body weight of 42.08±0.86 g. The study was arranged in a completely randomized design with 5 treatments and 4 replications. Each replication consisted of 8 birds. The treatments applied were T0 = basal ration, T1 = T0 + 0.05% GEUP, T2 = T0 + 0.10% GEUP, T3 = T0 + 0.15% GEUP and T4 = T0 + 0.20% GEUP. The parameters observed were protein digestibility, nitrogen retention, meat calcium and protein mass. The results showed that dietary addition of GUEP significantly (P <0.05) increased coefficient of protein digestibility, meat calcium and protein mass, but had no affect on nitrogen retention. The highest protein digestibility and meat calcium mass were shown at T4, but  it was not significantly different from T3, and T2 for protein digestibility. While the highest meat protein mass was at T2 but it was not different with T3 and T4. The conclusion of the study was that dietary supplementation of  glucomannan derived from porang tuber extract in broiler up to 0.15% (T3) increased protein digestibility, meat calcium and protein mass with similar nitrogen retention.

Growth response of leucaena embryogenic callus on embryo age differences and Auxin 2,4-Dichlorophenoxyacetic acid

Manpaki, Satria Julier ( Graduate School of Bogor Agricultural University ) , Prihantoro, Iwan ( IPB ) , Karti, Panca Dewi M.H. ( IPB )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 2 (2018): JUNE 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

Leucaena (Leucaena leucocephala cv. Tarramba) is a source of protein from the legume family. Tarramba varieties able to adapt well in tropical area such as West Nusa Tenggara and East Nusa Tenggara. This study aimed to identify the growth response and embryogenic callus morphology of lamtoro (L. leucaena cv. Tarramba) in embryos different ages and auxin 2,4-D levels. This research was used explants derived from lamtoro (L. leucocephala cv. Tarramba) as much 400 explants. this study conducted of 16 treatments with two factors, the first factor is the provision of PGR 2,4-D concentration of 0.5 mg L-1, 1 mg L-1, 1.5 mg L-1 and 2 mg L-1. The second factor was the type of embryo such as mature embryo, cotyledon, heart, and pre-globular. Each treatment was repeated 25 replications. This study uses a randomized complete design (CRD) with two factor. Data were analyzed using analysis of variance and if there was significant difference, data were further analyzed using Duncan’s multiple range test. Analysis of variance showed that PGR 2,4-D at a concentration of 1.5 mg L-1 and the type of optimum embryo was mature embryo callus on parameters as height, diameter increment callus, and callus color scores were significantly different (P <0.05). callus texture parameter indicates results that are uniform throughout the treatment that was compact callus. Lamtoro plant embryogenic callus (L. leucocephala cv. Tarramba) indicate an optimal response at the concentration of PGR 2,4-D 1.5 mg L-1 and the type of embryo was mature embryo. 

Clenbuterol Residue in Beef Meat Collected From Several Cities In Java Island, Indonesia

Widiastuti, Raphaella ( Indonesian Research Center for Veterinary Sciences (IRCVS) ) , Anastasia, Yessy ( ICRVS )

Indonesian Journal of Animal and Veterinary Sciences Vol 23, No 2 (2018): JUNE 2018
Publisher : Indonesian Animal Sciences Society

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Abstract

Clenbuterol (CLB) (see Figure 1) is an illegally animal drug of the β-agonist group that used as a promoter of growth in various farm animals. The presence of CLB residues in livestock products can cause poisoning in humans such as tremor, tachycardia, nausea and dizziness. The purpose of this research is to develop CLB residue detection method on beef detected using high performance liquid chromatography (HPLC) and to know the presence of CLB residue on 74 samples of frozen and fresh meat from several cities in Java. Samples were extracted with acetonitrile and isopropanol, then analyzed chromatographically using RDS ODS C18 column and mixed mobile phases of 50 mM NaH2PO4 and acetonitrile (80:20, v/v) and detected by photodiode array detector at 210 nm wavelength. The recoveries test at spiked concentrations of 2, 5 and 10 ng/g were 103.45, 89.27 and 89.53% for each additional concentrations. The detection limit and the quantitation limit were 0.10 ng/g and 0.31 ng/g, respectively. Analysis of 74 samples showed that CLB residue was detected in 8 samples in a concentration range of 2.40 to 15.06 ng/g and had exceeded the CLB residue maximum limit of 0.2 ng/g. To avoid the risk of the presence of CLB residues, it is necessary to regularly monitor the residue content of livestock food to ensure food safety for consumers.

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