Insisiva Dental Journal
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Distribusi Prevalensi Malaria di Puskesmas Kokap I dan Girimulyo I Kabuapten Kulonprogo Tahun 2002-2004 dan Hubungannya dengan Faktor-faktor Risiko

restu faizah

Insisiva Dental Journal Vol 2, No 2 (2011): 2
Publisher : Insisiva Dental Journal

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Abstract

Prevalence of malaria in Indonesia is still high, particularly in the areas of Java andBali. In Java, the health priority areas are endemic areas including mountain areas incise Kulonprogo district. This research aims to reveal the presence of risk factors associated withthe incidence of malaria in the area.This is a non-experimental research by reviewing the medical records of patients withmalaria in Primary Health Care in Kokap I and Girimulyo I in 2002-2004. Significance of therelationship between risk factors with the prevalence of malaria were analyzed by Chi-Square.The result show that malaria prevalence in Kokap I in 2002-2004 are 37,43%; 2,174%;0,246% respectively and in Girimulyo I are 3,632%; 0,183%; 0,013% respectively. Chi-squaresanalyze show that there is significant correlation (p<0,05) between age group, gender andthe occupation and the prevalence of malaria. There is unsignificant correlation betweeneducation level and the prevalence of malaria (p>0,05) in Kokap and Girimulyo.Key word: malaria prevalence, risk factors

HUBUNGAN ANTARA STATUS GIZI DENGAN STATUS ERUPSI GIGI MOLAR TIGA RELATION OF NUTRITION STATUS WITH ERUPTION THIRD MOLAR TEETH STATUS

Normayanti Sukma, Ana Medawati

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Erupsi gigi merupakan gerak normal gigi ke arah rongga mulut dari posisi pertumbuhannya dalam tulang alveolar. Banyak faktor  yang mempengaruhi erupsi gigi, salah satunya adalah nutrisi. Nutrisi sangat penting untuk pertumbuhan dan perkembangan seseorang, termasuk erupsi gigi. Tujuan penelitian adalah untuk mengetahui hubungan antara status gizi dengan status erupsi gigi molar tiga. Jenis penelitian ini adalah analytic descriptive dengan pendekatan cross sectional dengan melibatkan 90 mahasiswa Program Studi Kedokteran Gigi Universitas Muhammadiyah Yogyakarta yang diambil dengan metode consequentive sampling. Subyek penelitian terbagi dalam 3 kriteria IMT (indeks massa tubuh), yakni 30 mahasiswa dengan status gizi kurus, 30 mahasiswa dengan status gizi normal, dan 30 mahasiswa dengan status gizi gemuk.  Hasil analisis dengan menggunakan uji  statistic chi square menunjukkan hubungan  yang tidak signifikan P=0,092 atau P>0,05 sehingga dapat disimpulkan tidak terdapat hubungan antara status gizi dengan status erupsi gigi molar tiga.  

PENGARUH IMUNOGLOBULIN-Y TERHADAP PROTEIN STREPTOCOCCUS MUTANS YANG DIISOLASI DARI SUBJEK KARIES DAN BEBAS KARIES

Rizky Aditiya Irwandi, Endang Winiati Bachtiar, Mindya Yuniastuti

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

The main microbial culprit in dental caries is Streptococcus mutans (S.mutans), virulence of which can be observed by its differential protein expression between caries and caries-free subjects.The success of Immunoglobulin-Y (IgY) anti S.mutans as a passive immunization agent in eliminating S.mutans has been reported. The aim of this study is to analyze the effect of IgY anti S.mutans on the protein expression of S.mutans isolated from caries and caries-free subjects. Each dental plaque was collected by swabbing the buccal surface of the first lower permanent molar of caries and caries-free subjects. The plaques were then cultured on agar medium TYS20B. After 72 hours, the colonies from each of them were cultured in liquid medium TYS Broth for 72 hour. Each collected bacteria (whether from caries or caries-free subjects) were grouped into control and exposure group. In exposure group,  S.mutans  was exposed by pre-incubated (for one hour at 37°C) IgY  anti S.mutans for one hour at 37°C. Protein expression of S.mutans was analyzed with SDS PAGE after the preparation of its antigen and Bradford protein assay.  Our result shows that  S.mutans  41.3 kilodalton protein expression of caries subjects, are up-regulated in comparison to the control group. Meanwhile, the  S.mutans  41.3 kilodalton protein expression of caries-free subjects, are down-regulated in comparison to the control group. This study suggests that IgY anti S.mutans up-regulates 41.3 kilodalton protein expression of S.mutans  in the caries subjects. However IgY anti S.mutans down-regulates 4.13 kilodalton protein expression of S.mutans in the caries-free subjects.Keywords: Streptococcus mutans, protein expression of Streptococcus mutans,  imunoglobulin-Y anti Streptococcus mutans, caries and free caries subjects.

UJI TEMPERATUR AIR PENCAMPUR TERHADAP SETTING TIME BAHAN CETAK KULIT BUAH MANGGIS (Garcinia mangostana)

Dian Yosi Arinawati Yosi Arinawati, Andi Triawan

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

  Bahan cetak gigi adalah bahan yang digunakan untuk membuat  replica  (model) gigi dan jaringan sekitarnya. Bahan cetak harus memenuhi syarat, salah satunya harus memiliki setting time yang cukup sehingga operator dan pasien dapat melakukan pencetakan gigi dengan baik. Penentuan waktu gelasi bisa ditentukan oleh beberapa faktor yaitu temperatur air, W/P ratio dan bahan pengisi yang dipakai. Namun cara modifikasi tersebut banyak memberikan efek pada sifat gel seperti elastisitas, dan mempengaruhi kekuatan terhadap robekan. Cara lain yang lebih aman ialah dengan mengubah temperatur air pencampur. Pada bahan cetak alginat, terbukti bahwa semakin tinggi temperatur, semakin pendek waktu gelasi. Namun belum ada pembuktian bahwa sifat-sifat tersebut berlaku untuk bahan cetak kulit Buah Manggis (Garcinia mangostana). Penelitian ini bertujuan untuk mengetahui bagaimana pengaruh temperatur air pencampur terhadap setting time bahan cetak kulit Buah Manggis (Garcinia mangostana).  Dua puluh lima spesimen dibagi menjadi 5 kelompok : kelompok 1 (10°C), kelompok 2 (15°C), kelompok 3 (20ºC), kelompok 4 (25°C) dan kelompok 5 (30°C). Pengukuran setting time menggunakan alat ukur indikator setting time berbentuk batang silindris dari bahan poly (methyl methacrylate).   Hasil uji statistik ANAVA satu jalur menunjukkan bahwa temperatur air pencampur berpengaruh terhadap  setting time  bahan cetak kulit buah Manggis (Garcinia mangostana) (p < 0,05). Hasil uji LSD 0,05 menunjukkan adanya perbedaan setting time yang bermakna antar setiap kelompok tempetatur air pencampur yang digunakan, kecuali pada kelompok temperatur air pencampur 20°C dan 25°C. Kedua kelompok temperatur air pencampur tersebut tidak menunjukkan perbedaan yang bermakna karena kedua kelompok temperatur air pencampur tersebut berada pada temperatur air pada suhu ruangan. Kesimpulan dari penelitian ini adalah temperatur air pencampur suhu 10°C, 15°C, 20°C, 25°C dan 30°C mempengaruhi lama  setting time bahan cetak kulit Buah Manggis (Garcinia mangostana) dan semakin tinggi temperatur air pencampur yang diuji,  setting time  semakin pendek (cepat). Setting time  tercepat terdapat pada kelompok suhu (temperatur) air pencampur 30° C.  Kata kunci : bahan cetak kulit buah Manggis (Garcinia mangostana), setting time, temperatur air

PENGARUH EKSTRAK ANTANAN (Centella asiatica) DIBANDINGKAN DENGAN IBUPROFEN TERHADAP KADAR HCL GASTER TIKUS

Yudha Perwira Putra, Euis Reni Yuslianti

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Latar Belakang dan Tujuan:  Ibuprofen merupakan salah satu obat anti inflamasi yang diketahui memiliki efek samping terhadap peningkatan kadar HCl lambung. Salah satu obat tradisional yang memiliki efek anti inflamasi adalah antanan (Centella asiatica). Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak antanan dibandingkan dengan ibuprofen terhadap kadar HCl gaster tikus. Bahan dan Metode:  Penelitian ini menggunakan metode eksperimental laboratorik dengan 30 ekor tikus wistar jantan. Kelompok perlakuan terbagi atas kelompok yang diberi ektrak  antanan 20 mg/200 gr berat badan/oral, kelompok yang diberi ibuprofen 21,6 mg/200 gr berat badan/oral dan kelompok kontrol. Perlakuan diberikan selama 7 hari dan setelah pemberian perlakuan (hari ke-8), tikus dipuasakan selama 24 jam kemudian dilakukan pengangkatan lambung dan dilanjutkan dengan pengambilan getah lambung untuk mengetahui kadar pH dengan menggunakan metode pH meter serta metode titrasi.  Hasil dan Analisis:  Hasil penelitian didapatkan kelompok yang diberikan  ekstrak antanan (Centella asiatica) meningkatkan asam lambung dengan rata-rata  acidity  pH 1,15 sedangkan pada kelompok yang diberikan ibuprofen meningkatkan asam lambung dengan rata-rata acidity pH 1,06. Hasil analisis statistik menggunakan metode One Way ANOVA dan dilanjutkan uji lanjut Duncan (p ≤ 0.05) menunjukan bahwa ekstrak antanan dan ibuprofen mempunyai pengaruh terhadap penurunan kadar asam lambung (p =  0.00).  Kelompok perlakuan ibuprofen memberikan pengaruh yang berbeda dibandingkan dengan yang lainnya, sedangkan pada kelompok perlakuan  kontrol  memberikan pengaruh yang sama dengan kelompok perlakuan ekstrak  antanan. Kesimpulan: Penelitian ini dapat disimpulkan bahwa pemberian ekstrak antanan mempunyai pengaruh lebih rendah dibandingkan dengan pemberian ibuprofen dalam meningkatkan kadar HCl lambung tikus.   Kata kunci : antanan, ibuprofen, kadar HCl, pH  

PENGARUH PENAMBAHAN PATI UBI KAYU PADA BAHAN CETAK ALGINAT TERHADAP STABILITAS DIMENSI

Mirna Febriani

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Bahan cetak alginat merupakan bahan  yang digunakan untuk mencetak gigi geligi dan jaringan rongga mulut ,bahan cetak alginat masih di impor dan cukup mahal harganya. Pada penelitian Febriani 2001, telah dilakukan modifikasi pada bahan cetak alginat dengan pati ubi  kayu dengan perbandingan 1:1 dan didapat hasil reproduksi detil yang sama dengan bahan cetak alginat tanpa ditambah dengan pati ubi kayu.  Penelitian ini bertujuan  untuk menganalisis sifat  stabilitas dimensi   bahan cetak alginat yang ditambah pati kayu. Pati ubi (Manihot utilisima) merupakan suatu polimer yang mengandung amilosa dan amilopektin yang dapat ditambahkan pada bahan cetak alginat. Metoda dan bahan.Bahan yang digunakan pada penelitian ini adalah bahan cetak alginat tipe normal, pati ubi kayu, aquadestilata dan alat uji  stabilitas dimensi  sesuai standar ANSI/ADA no.18/1992.Hasil penelitian. Hasil  stabilitas dimensi    stabilitas dimensi antara bahan cetak alginat standar (2,9782 mm dan 2,9719 mm) dengan bahan cetak alginat standar yang ditambah pati ubi kayu (2,9797 mm dan 2,9795 mm),. Kesimpulan.Penambahan pati ubi kayu (Manihot utilisima) pada bahan cetak alginat standar mendapatkan hasil pengujian stabilitas dimensi   yang masih memenuhi standar ANSI/ADA no.18/1992. Stabilitas dimensi bahan cetak alginat yang ditambah pati ubi kayu memiliki nilai stabilitas dimensi  yang lebih lama dari pada bahan cetak alginat standar.  

THE DECREASE IN NUMBER OF BLOOD POLYMORPHONUCLEAR (PMN) TO PERIAPICAL RADIOGRAPHS DOSE OF RADIATION EXPOSURE

Amni Adlina, Wasilah Wasilah

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Background: Periapical radiograph is a type of radiographic examinations that is widely used in the field of dentistry. Radiographic X-ray is an ionizing radiation that can cause damage and death to cells, tissues or organs, including the polymorphonuclear (PMN). PMN is cell that serves as the first line of defense against the invasion of organisms. Objective: The purpose of this study is 1) to determine whether there is a decrease in the number of peripheral blood PMN after  being given periapical radiograph dose of  X-ray radiation exposure, and 2) to determine whether there is any difference in the declining number of  peripheral blood PMN after being given  a single dose and  some different total dose of periapical radiograph of  X-ray radiation exposure. Methode: The design of this study is an experimental laboratory research with total sample of 24 strains of Balb c male mice. The sample is divided into four groups, each consisting 6 mice. Group 1 serves as the control group; Group 2 is given a treatment with a single-dose exposure of periapical radiographs of X-ray radiation; Group 3 is given a treatment of  6 times total doses exposure of periapical radiograph of X-ray radiation; and Group 4 is given a treatment of 14 times total doses exposure of periapical radiograph of X-ray radiation. After 24 hours of radiation exposure, the blood was drown. Conclusion : The calculation is done by multiplying the number of PMN percentage  from leukocyte counts with a total leukocyte. The data obtained  are  statistically tested using One Way Anova and LSD with a significance level of 95%. Result : The result of this study indicates that there is a difference  in the peripheral blood PMN counts in male mice  after being exposed  to radiation doses of periapical radiographs in all groups (P <0.05). There is an increasing number of peripheral blood PMN in male mice  after being treated with X-ray radiation at a  single  dose of periapical radiographic exposure, whereas after being exposed with radiation at the dose of 6 and 14, the number of PMN is declining. The highest decreasing number of PMN is found in the group with exposure of radiation at 14 dose.                                               Keywords: Polymorphonuclear (PMN), X-ray Radiation, Periapical Radiograph 

THE EFFECTIVENESS OF TOFU LIQUID WASTE AS A NATURAL PHYTOESTROGEN FOR MANDIBULAR BONE OF OVARIECTOMIZED RATS

Meilia Aquina, Agustine Hanafi, Nungky Devita

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Background  :  Estrogen is a steroid compound that has many benefits for  the bodys physiology. Hypoestrogenic women could have periodontitis in their mouth. Hormone replacement therapy (HRT) is a therapy  that  used to reduce  the  disturbance caused by estrogen deficiency.  However, HRT has many side effects.  Theoretical study shows that isoflavones  in tofu liquid waste has an element of a steroid compound. The purpose of this study was to prove the effectiveness of isoflavones in the tofu liquid waste by examining the microscopic structure, estrogen receptor expression and MDA levels in rat mandibular bone post-ovariectomy. This research method use a true experimental laboratory with randomized post test control group design.  Methode:  Twenty-four female wistar rats were divided randomly into six groups, consisted  of a group of normal  rat  that  does’nt ovariectomized (C1), 4 weeks ovariectomized rats (C2), 8 weeks ovariectomized rats (C3) but no tofu liquid waste was given, and 8 weeks ovariectomized rats that given tofu liquid waste at the end of the 4th  week of ovariectomy with three different doses (C4 = 1.2, C5 = 6; C6 = 12 ml / kg). Histopathological slide  used to see the changes resulted in a number of osteoclasts, osteocytes, periodontal ligament width, alveolar bone height, the amount of estrogen receptor expression and MDA levels.  Result:  The results of statistical tests  show that  there is a difference in the rats between six different groups (ANOVA, p <0.05) and there is a close relationship between  the dose of the tofu liquid waste  with all parameter. Condlusion: The conclusion  of this study is tofu  liquid waste  could  improve the microscopic structure, estrogen receptor expression and MDA levels in rat mandibular bone post-ovariectomy.  Keywords: Menopause, Periodontitis, Isoflavones, Tofu Liquid Waste 

THE EFFICACY OF GLASS IONOMER HYBRID AS ORTHODONTIC BRACKETS ADHESIVES TO INHIBIT CALSIUM RELEASED OF THE ENAMEL

Rina Sutjiati

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Background : Composite resins are predominantly used to bond orthodontic brackets onto teeth. These can lead to calcium released of the enamel surrounding the brackets. Purpose  : To observe calcium release during the use of adhesives which release fluoride Method  : This experimental study was design to determine the calcium released after soaking in the acid solution. This in vitro study used forty extracted human premolars (20 teeth were bonded with glass ionomer, and 20 teeth were bonded with glass ionomer hybrid). Half of the teeth in each group were treated  with fluoride application and the other half were not treated fluoride application topically. Calcium released of the enamel was evaluated using Spectrofotometer. Result : The results showed that there was a significant difference between glass ionomer group without and with fluoride application (p<0,005), and there was not significant difference (p>0,005) in the group of glass ionomer hybrid without and with fluoride application topically. Conclusion : The adhesive glass ionomer hybrid could prevent calcium released with or without the usage of sodium fluoride.  Key words : topical application, adhesive bracket, calcium released.  

THE GROWTH OF BODY LENGTH AND WEIGTH OF MALE WISTARS SUPPLIED WITH ANCHOVIES (Stolephorus sp.) AS ADDITIONAL DIET

Tecky Indriana

Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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Abstract

Background: Growth is affected by nutrition contents in the diet consumed. Nutrition playing role in the growth process is carbohydrate, protein, fat, mineral, water, and vitamins. Calcium is one of important minerals involving in cell metabolism process needed in the process of growth and development. One of sources of  animal calcium is anchovy, besides its cheap price, anchovies may be consumed wholy together with their bones. The purpose of this study was to observe whether the supply of anchovies as additional diet may increase the growth of male wistars.  Methods:  A number of 12 male wistars divided into two groups, control and experimental groups. The control group was only supplied with standard diet and sterile aquades, while the other group was supplied with anchovies as additional diet through oral sondage of 0,00062 gr/day x BW (Body Weigth) for 40 days.  Every wistar in each group was subsequently measured for its body length and weigth once a week to observe its growth and development. The data obtained were analized using anova test and LSD orderly. Results: The result demostrated the increase in the body length and weigth of the male wistars of both groups in each week. Anova statistical test that was continued with LSD test showed that there was significant increase (p<0,05) in the experimental group in each week compared to the control group. Conclusion: The supply of anchovies(Stolephorus sp) as additional diet may increase the growth of male wistars body length and weigth.   Key word :  anchovies (Stolephorus sp), growth and development, protein