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Biogenesis
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Articles 180 Documents
Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul K, Mahfut; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest
Kontaminasi Bakteri Pada Saus Tomat Mie Ayam di Pasar Condong Catur Sleman Yogyakarta Tahun 2013 Nadifah, Fitri; Yasintha Bhoga, Maria; Prasetyaningsih, Yuliana
Biogenesis Vol 2, No 1 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

The tomato sauce is a complementary food that is often served with other foods, such as chicken noodles. Various factors can be the cause of contamination of tomato sauce by microbes like bacteria. These factors include environmental conditions that are not clean or container that was left open. Bacterial contamination in tomato sauce can result not suitable for consumption. Given by the high public interest in the consumption of tomato sauce with chicken noodle, it is necessary to observe the bacterial contamination of chicken noodle tomato sauce. To determine the bacterial contamination in tomato sauce of chicken noodle in traditional market of Condong Catur, Sleman, Yogyakarta. The samples used were tomato sauces that are used by chicken noodle stalls in the Condong Catur traditional market. Sampling was also carried out by recording the environmental conditions stalls and tomato sauce serving. To determine the bacterial contamination, samples examined in the laboratory based on the total bacterial count method using plate count agar (PCA) media. Seven of the ten samples showed the total number of bacteria <104 CFU/ml, whereas the other three samples are not suitable for consumption by the total number of bacteria >104 CFU / ml. The main factors of high contamination is poor hygiene of chicken noodle stall environmental, tomato sauce containers that are less clean and are often left open.Keywords: tomato sauce, total bacteria
Pertumbuhan Tiga Kultivar Padi (Oryza sativa L.) Terhadap Aplikasi Pupuk Bio Cair dan Kondisi Tanah Pertanian Pasca Erupsi Merapi 2010 Umi Siswanti, Dwi; Rachmawati, Diah
Biogenesis Vol 1, No 2 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

The eruption of Mount Merapi at the end of 2010, which resulted in the destruction of land and crops are changing wetland into dry land. Sleman which is located south of Mount Merapi became one of the areas that most affected by the eruption of Merapi in 2010. It is necessary to improve the processing of soil physical properties of degraded areas and efforts to restore agricultural soil nutrient. Returning soil nutrients can be cultivated with the addition of organic material or the addition of microbes. The research aims to analyze the growth of three cultivars of rice (Oryza sativa L.) againstthe application of biofertilizer in paddy fields were affected by the eruption of Merapi in 2010. These three rice cultivars are Situbagendit, Inpari 20 and Ciherang. This research was conducted by applying the biofertilizer as much as 10 liters / ha were given three times during the vegetative phase, ie: 7, 21and 35 days after planting. The results of this research indicate that the three rice varieties ie: Situbagendit, Ciherang and Inpari 20 have an optimal growth and an increase in soil nutient content in biofertilizer application.Keywords: biofertilizer, degraded land, Merapi eruption, rice
Analisis Multidrug Resistensi Terhadap Antibiotik Pada Salmonella typhi Dengan Teknik Multiplex PCR Evi Erviani, Andi
Biogenesis Vol 1, No 1 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

This research is about the analysis of resistance multidrug for antibiotic of Salmonella typhi with multiplex technique of PCR. This research purpose conducted to determine the presence of resistance multidrug for kloramfenikol and ampicillin of Salmonella typhi using multiplex technique of PCR. Testing of resistance multidrug performed using disc diffusion test and detection of resistance gene for kloramfenikol (cat P) and ampicillin (tem) with molecular technique using multiplex of PCR. Result of research indicate the suitability between resistance test using disc diffusion and detection resistance gene with multiplex of PCR, which is based on result of disc diffusion test and multiplex of PCR have occurred of resistance multidrug for kloramfenikol and ampicillin antibiotics, amount 10 patients (33.3%), resistance of one antibiotics amount 10 patient (33.3%) and non-resistance (sensitive) for both antibiotic amount 10 patient (33.3%).Keywords: Antibiotics, Multidrug, Resistance, Salmonella typhi
Karakter Genetik Populasi Bedeng 61B Desa Wonokarto Kabupaten Lampung Timur Pasca Program Kolonisasi Pemerintah Belanda Nur Khoiriyah, Yustin
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Gene and genotype frequencies are important in a population characterizing. Based on the frequency of these gene and genotype, distinctiveness of population can be determined (Arisuryanti et al., 2007). Gene frequencies of population can be changed if there are evolutionary forces (the factors that play a role in changing the allele and genotype frequencies), such as, mutation, migration, non-random mating, genetic drift and natural selection (Arisuryanti & Daryono, 2007). Migration and genetic drift were thought to be a factor that changed alleles and genotype frequencies in the population of Lampung. Witrianto (2010) showed migration programs that were triggered by the Dutch government, it was called colonization. Therefore, in the population of Lampung, indication of changes in allele and genotype frequencies were need to be evaluated. The results showed that frequency of alleles IA, IB and IO in the population were 0.17; 0.21 and 0.62, respectively. Frequency of genotype IAIA, IAIO, IBIB, IBIO, IAIB and IOIO were 0.290; 0.2108; 0.0441; 0.2604; 0.0650 and 0.3800, respectively. Chi Square test showed that the value of X2 were 0,07 (X2 < α critical value at 5% for dF = 3). It can be concluded that alleles and genotype distribution of ABO blood system in the population were in accordance with Hardy Weinberg equilibrium law, so that the equilibrium legal status were applied in this population. Genetic drift and gene flow were not affected alleles and genotype changes. The process of microevolution were not occur in the population of people from the 61 B plot of Wonokarto village.Keywords: character of genetic population, post-colonization of Dutch government, the 61 B plot ofWonokarto village
Karakter Fenotipik Tanaman Stroberi Festival (Fragaria x ananassa D.) Hasil Induksi Kolkisin Pada Konsentrasi 0,05% dan 0,01% Riza Aristya, Ganies; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Strawberries were a lot of holticulture commodities consumed by the people of Indonesia. Strawberry consumption continues to increase over time in Indonesia but not matched by production. This it because the strawberry is native to subtropical climate so that production in Indonesia limited in the plateau regional still affected by the tropical climate. Therefore, it is necessary to increase the production of both quality and quantity of strawberries, one with plant breeding through the induction of colchicine. The objective of this research were to determine the differences in phenotypic characters of strawberry plants with the control and induction results to determine the length of time and the induction colchicine 0,05% and 0,01% on the Festival cultivar of strawberry most effective. Research were done by inducing with 0,05% and 0,01% colchicine in leaves, roots 24 hours and 36 hour, the root and leaves of 24 and 36 hour. The observed for 6 months long leaf growth, leaf width,number of leaves, stem circumference, plant height, bloom vast flower and volumes of the fruit. The results indicate that the induction of the leaf growth at the optimal parameter leaf lenghth, leaf width and number of leaves. While 24-hour induction of root growth at optimum height and volume of fruitplants, then root induction at 36 hour to flowers bloom. Induction in root and leaves 36 hour optimum growth on trunck circumference. From this study it could be concluded that there are differences in the character of the strawberry crop cultivar Festival phenotype induction results colchicine 0,05% and 0,01% with control. Induction for 36 hours and induction on leaves was most effective for induction of strawberry cultivar Festival.Keywords: character phenotype, colchicine, festival
Uji Aktivitas Antibakteri Ekstrak Kloroform dan Ekstrak Etanol Biji Bidara Laut (Strychnos ligustrina Bl) Terhadap Staphylococcus aureus ATCC 25923 dan Salmonella thypi Sumiati, Eti
Biogenesis Vol 2, No 1 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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A research has been conducted on the antibacterial activity of Strychnos ligustrina seeds against Staphylococcus aureus ATCC 25923 in vitro. Strychnos ligustrina seeds extracted using two solvents, namely chloroform (chloroform extract) and 70% ethanol (ethanol extract) and analysis of the chemical content of the extract by thin layer chromatography and paper chromatography. The results of this study are expected to provide data activity of Strychnos ligustrina seeds as antibacterial scientifically. In this study, the chloroform extract and ethanol extract of Strychnos ligustrina seeds obtained by the filtration method with Soxhlet apparatus. Antibacterial activity test of chloroform extract and ethanol extract 70% from Strychnos ligustrina seeds performed using liquid dilution method, the parameters used are the MIC (minimum inhibitory concentration) and MKC (minimum killing concentration). MIC is determined by turbidity or clarity of the test solution, while MKC determined by scraping the respective concentrations of the extract / test solution on blood agar medium. The results showed that the chloroform extract and ethanol extract of Bidara Laut seed has antibacterial activity against Staphylococcus aureus ATCC 25923 and Salmonella thypi. MIC of each test extracts (chloroform and ethanol 70%) could not be determined because both of the test extract produce turbid color, while the MIC of the second test extract was 3.62% b/v for Salmonella thypi, 8.75% for Staphylococcus aureus ATCC 25923 (Strychnos ligustrina seeds chloroform extract), 22.5% for Salmonella thypi and 15% for Staphylococcus aureus ATCC 25923 (Strychnos ligustrina seeds ethanol extract). Results of thin layer chromatography and paper chromatography showed that the chloroform extract seeds contain alkaloids and steroids / triterpenoids, whereas the 70% ethanol extract contains alkaloids, tannins and steroids/triterpenoids. Keywords: antibacterial, chloroform extract, ethanol 70% extract, Strychnos ligustrina seeds, Salmonella thypi, Staphylococcus aureus
Penggunaan Ampas Batang Pisang Terhadap Pertumbuhan Ikan Mas di Desa Salutambun Barat Kabupaten Mamasa Alang, Hasria
Biogenesis Vol 1, No 2 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

The research of the influence of the banana steam residues as pellet for Cyprinus carpio growth has been done at west Salutambun village, Buntu Malangka Districts, Mamasa Counties. The type of this research is experimental study and the aim of this study to see the effect of alternative pellet for Carp (Cyprinus carpio) growth. The research method include the manufacture and testing of alternative pellets for carp (Cyprinus carpio). Alternative pellets consist of banana stem as the main ingredient, a banana peel, fine bran and sweet potato leaves. Everything yielded then mixed with rice water. The pellets are then dried and cured for one night and then milled to from a circle with a small sircle. The data analysis technique used is completely randomized (RAL) is fine that the F value 14,85** is greater than F table α 0,01 with value 13,74 (F hitung > F tabel). Then there is a very significant effect residual use in the manufacture of banana pellets for carp growth at West Salutambun village, Buntu Malangka Districts, Mamasa Counties.Keywords: Cyprinus carpio (carp), growth, pellets
Analisis Pedigree dan Fenotip Pasangan Kembar: Studi Kasus Pada Keluarga Kembar di Kecamatan Laweyan, Surakarta Machwiyah, Yuyun; Satuti Nur Handayani, Niken
Biogenesis Vol 1, No 1 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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The twin pregnancy is a kind of pregnancy with two or more fetuses. Basically, the twins can be grouped of two, they are fraternal twins (dizygotic) and identical twins (monozygotic). Fraternal twin is a kind of twins that comes from two ova, which each of them will be fertilized by the sperm. While, identical twin comes from an ovum, which fertilized by a sperm. This research is conducted in order to observe the inheritance pattern from the pedigree chart, and to observe similarity and dissimilarity phenotype between twin couple on families observed. Methods used for this research are data collection to construct pedigree, phenotype analysis to compare the similarity and dissimilarity phenotype between the twins that are observed, paraffin method to observe the form of hair from cross section and agglutination reaction to observe the blood group from the twins. Based on pedigree chart of twins in Laweyan, Surakarta, it seems that the identical twins is passedfrom generation to generation, and on the family observed the inheritance pattern of identical twins birth process is come from the women of identical twins. The lobe of ear from each twins there is a twin couple showing the different lobe. There is any similarity and dissimilarity relate to form of hair cross section from each couple. The blood group of each couple are showing the same result.Keywords: identical twins, inheritance pattern, pedigree chart
Induksi Tunas dan Perakaran Bambu Kuning Bambusa vulgaris secara in vitro Astuti, Puji
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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An experiment to induction shoots and rooting of the shoot of yellow bamboo Bambusa vulgaris have been carried out. Single nodal segments with axillary buds were the starting material. After sterilization, nodal segments were directly inoculated on modified Murashige & Skoog (MS) medium, supplemented with cytokinin Benzylaminopurine (BAP) with various concentration (0,0 ppm, 0,5 ppm, 1,0 ppm and 2,0 ppm) and auxin Indole butyric acid (IBA) with various concentration (0,0 ppm, 2,5 ppm, and 5,0 ppm). The results of the experiment showed the combination BAP 1,0 ppm and IBA 2,5 ppm has effectively induced shoot multiplication. Meanwhile, the used BAP 2,0 ppm and IBA 2,5 ppm resulted in the best shoot elongation. On the other hand, rooting of the shoots in vitro have not been successful yet.Keywords: BAP, IBA, induction shoots and rooting, in vitro, yellow bamboo

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