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Biogenesis
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Articles 20 Documents
Search results for , issue " Vol 2, No 2 (2014)" : 20 Documents clear
Profil Hematologi Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4, 6, dan 8 Minggu Fitria, Laksmindra; Sarto, Mulyati
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Animal model is very important in biomedical research. Prior to apply in human or other primates, experiments using lower animals model such as rodents must be conducted (preclicical studies). Wistar rats (Rattus norveg
Struktur Histologis Hepar, Intestinum, dan Ren Burung Cerek Jawa (Charadrius javanicus Chasen 1938) Dengan Kontaminasi DDT di Delta Sungai Progo Yogyakarta Puspita Sari, Dewi; Hadisusanto, Suwarno; I, Istriyati
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Residues of DDT (Dichloro Diphenyl Trichloroethane) in the river environment can get into the Birds body through an intermediary Javan Plover natural feed. Residues of DDT will experience biomagnification and biotransformation in the body organism. The purpose of this research was to determine residue of DDT in the Progo’s mudflat region, several organs of birds, and their impact on liver, intestine, and kidney Javan Plover. This research was located in Progo’s downstream. The method of environment and feed sampling done directly on site. The bird sampling using mist nets. Detection of residues DDT in environment samples by gas chromatography. Measurement of DDT residues in feed and organs of birds with spectrophotometer. Preparation of histological slide from liver, intestine, and kidney using paraffin method (with Hematoxilin & Eosin and Mallory Acid Fuchsin staining). Quantitative Data obtained from the measurement results of environment, feed,and organs of Javan Plover samples. Qualitative data analize by descriptively. The analysis showed DDT residues detected in area’s Progo downstream, natural feed and Javan plover’s organ. Analysis shows chronic effects posed by DDT residue on the histological structure of liver, intestine, and kidney of Javan Plover.Keywords: DDT (Dichloro Diphenyl trichloroethane), histological structure, Javan Plover(Charadrius Javanicus Chasen 1938), Progo’s River
Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul K, Mahfut; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest
Karakter Genetik Populasi Bedeng 61B Desa Wonokarto Kabupaten Lampung Timur Pasca Program Kolonisasi Pemerintah Belanda Nur Khoiriyah, Yustin
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Gene and genotype frequencies are important in a population characterizing. Based on the frequency of these gene and genotype, distinctiveness of population can be determined (Arisuryanti et al., 2007). Gene frequencies of population can be changed if there are evolutionary forces (the factors that play a role in changing the allele and genotype frequencies), such as, mutation, migration, non-random mating, genetic drift and natural selection (Arisuryanti & Daryono, 2007). Migration and genetic drift were thought to be a factor that changed alleles and genotype frequencies in the population of Lampung. Witrianto (2010) showed migration programs that were triggered by the Dutch government, it was called colonization. Therefore, in the population of Lampung, indication of changes in allele and genotype frequencies were need to be evaluated. The results showed that frequency of alleles IA, IB and IO in the population were 0.17; 0.21 and 0.62, respectively. Frequency of genotype IAIA, IAIO, IBIB, IBIO, IAIB and IOIO were 0.290; 0.2108; 0.0441; 0.2604; 0.0650 and 0.3800, respectively. Chi Square test showed that the value of X2 were 0,07 (X2 < α critical value at 5% for dF = 3). It can be concluded that alleles and genotype distribution of ABO blood system in the population were in accordance with Hardy Weinberg equilibrium law, so that the equilibrium legal status were applied in this population. Genetic drift and gene flow were not affected alleles and genotype changes. The process of microevolution were not occur in the population of people from the 61 B plot of Wonokarto village.Keywords: character of genetic population, post-colonization of Dutch government, the 61 B plot ofWonokarto village
Karakter Fenotipik Tanaman Stroberi Festival (Fragaria x ananassa D.) Hasil Induksi Kolkisin Pada Konsentrasi 0,05% dan 0,01% Riza Aristya, Ganies; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Strawberries were a lot of holticulture commodities consumed by the people of Indonesia. Strawberry consumption continues to increase over time in Indonesia but not matched by production. This it because the strawberry is native to subtropical climate so that production in Indonesia limited in the plateau regional still affected by the tropical climate. Therefore, it is necessary to increase the production of both quality and quantity of strawberries, one with plant breeding through the induction of colchicine. The objective of this research were to determine the differences in phenotypic characters of strawberry plants with the control and induction results to determine the length of time and the induction colchicine 0,05% and 0,01% on the Festival cultivar of strawberry most effective. Research were done by inducing with 0,05% and 0,01% colchicine in leaves, roots 24 hours and 36 hour, the root and leaves of 24 and 36 hour. The observed for 6 months long leaf growth, leaf width,number of leaves, stem circumference, plant height, bloom vast flower and volumes of the fruit. The results indicate that the induction of the leaf growth at the optimal parameter leaf lenghth, leaf width and number of leaves. While 24-hour induction of root growth at optimum height and volume of fruitplants, then root induction at 36 hour to flowers bloom. Induction in root and leaves 36 hour optimum growth on trunck circumference. From this study it could be concluded that there are differences in the character of the strawberry crop cultivar Festival phenotype induction results colchicine 0,05% and 0,01% with control. Induction for 36 hours and induction on leaves was most effective for induction of strawberry cultivar Festival.Keywords: character phenotype, colchicine, festival
Induksi Tunas dan Perakaran Bambu Kuning Bambusa vulgaris secara in vitro Astuti, Puji
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

An experiment to induction shoots and rooting of the shoot of yellow bamboo Bambusa vulgaris have been carried out. Single nodal segments with axillary buds were the starting material. After sterilization, nodal segments were directly inoculated on modified Murashige & Skoog (MS) medium, supplemented with cytokinin Benzylaminopurine (BAP) with various concentration (0,0 ppm, 0,5 ppm, 1,0 ppm and 2,0 ppm) and auxin Indole butyric acid (IBA) with various concentration (0,0 ppm, 2,5 ppm, and 5,0 ppm). The results of the experiment showed the combination BAP 1,0 ppm and IBA 2,5 ppm has effectively induced shoot multiplication. Meanwhile, the used BAP 2,0 ppm and IBA 2,5 ppm resulted in the best shoot elongation. On the other hand, rooting of the shoots in vitro have not been successful yet.Keywords: BAP, IBA, induction shoots and rooting, in vitro, yellow bamboo
Strategi Seleksi Tempat Bertelur Burung Mamoa (Eulipoa wallacei Gray, 1860) di Kecamatan Galela Ahmad, Zulkifli
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

North Mollucas is the main of priority areas for global biodiversity. Not the entire beach in the Galela district be created nesting ground by birds of Mamoa. This is caused to existence of the key factors that the criteria for site selection, for example physical structure, safe location, and flanked by two barriers. The purpose of this study was to investigate the characteristics of nesting ground, vegetation composition and study the physical structure of the nesting grounds in the Galela district. Primary data were collected ; field characteristics of nesting ground, dimension and pattern layout eggs hatch hole, soil physical and chemical (temperature, moisture, aeration, pH, and organic matter content). To examine the relationship between temperature with depth, with a humidity range of the highest tides, used of simple regression analysis. Vegetation analysis conducted qualitative description by calculating the parameters of vegetation and Important Values. The method used for vegetation data are squares method designed in the form of a belt in a transect (bisect). The results showed that Mamoa birds make nests hatch for protection and convenience for cheepers. The temperature, moisture, aeration, the distance between the nest, the nest distance between the highest and the content of organic matter, is not a key factor in the site selection by the parents, but the determining factor in the process of egg incubation. The composition of the vegetation found on the beach Mamuya consists of 22 species, 13 species on Tiabo beach, and 17 species on the beach of Denamabobane. Stratification vegetation of tree level is dominated by Terminalia cattapa, and Hibiscus tiliaceus. At the level of the pole is Calothropis gigantera Dryand, Avicenia Sp., Caesalpinia bonduc, Terminalia cattapa, and Mangifera indica. At stake level is Terminalia cattapa, Scaevolia tacada, Rhizopora appiculata, Citrus aurantifolia and Mangifera indica. At the level of the shrubs is Bruguiera gymnorrhiza Lamk, Scaevolia taccada, Ficus septica, Anona muricata, and Morinda citrifolia, and undergrowth is Hypolitrium, Ipomea pescaprae, Phaseolus lunatus, Phaseolus foetida and Andropogon acciculatus Ritz.Keywords: Galela district, Mamoa birds, nesting ground, strategy, selection
Uji Teratogenitas Ekstrak Bungo Timah (Peperomia pellucida L. Kunth.,) Terhadap Organ Viseral Embrio Mencit Putih (Mus musculus L.) Sari, Yelvita; Munir, Warnety; Allen, Yohannes
Biogenesis Vol 2, No 2 (2014)
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Abstract

The study on terratogenict effect of Bungo Timah (Peperomia pellucida L. Kunth.,) extract to visceral organ of mice embryo (Mus musculus L) was conducted to identify the effect of P. pelucida extract to development of mice embryo visceral organ. The Complete Randomize Method, which consist of four, extract dose variation: 0; 75; 100; 125 mg/kg BW given from the day 6th-4th of pregnancy were used. The result showed the extract of P. pellucida was not teratogenic at 75 mg/kg BW. While at dose 100 and 125 mg/kg BW showed visceral organ malformation, the cardiac enlargement, right kidney fission, intestinal diverticula, even thought statistically was not significant (P<0,05).Keywords: Mus musculus, organ viseral, Peperomia pellucida (L.) Kunth, teratogenitas
Respons Fisiologis Padi (Oryza sativa L.) “Segreng” dan “Menthik Wangi” Terhadap Aplikasi Pupuk Organik Cair dan Dekomposer Umi Siswanti, Dwi; Virgiyana Agustin, Rega
Biogenesis Vol 2, No 2 (2014)
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Abstract

The background of the community services at Sruni (a part of Wukirsari village) is our concern in degraded land at around of Mount Merapi after eruption 2010. Researcher encourages people of Wukirsari village to self-sufficient in the supply of organic manure for their fields. It is intended to restore the soil nutrients to improve the soil structure. The target of this activity are increasing agricultural productivity, production costs and improving the efficiency of agricultural cultivation. The conclution of this research and community services are biofertilizer producted by Sruni’s people response positively to the growth of rice plants especially in combination between biofertilizer and vegetative and generative decomposers. The combination of biofertilizer and vegetative decomposers gives the effect of increase of leaf chlorophyll content in both varieties of rice plants (Menthik Wangi and Segreng). The combination also gives a striking response to the increase in Activity of Nitrate Reductase (ANR) of the flag of leaf of Segreng plants. Overall ,Researchers have implementing more than 90% of the planned activities in this grant.Keywords: biofertilizer, decomposers, degraded land, Menthik Wangi, rice, Segreng
Isolasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Bonggol Nanas (Ananas comosus) pada Variasi Suhu dan pH Masri, Mashuri
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

This study was conducted to isolate the bromelain enzyme from pineapple weevil (Ananas comosus) and measure the protein and enzyme activity of bromelain with gelatin substrate. Research stage involves determining the protein content of the bromelain enzyme in the treatment of ammonium sulfate precipitation by 10-60% concentration and determination of bromelain enzyme activity at various pH 4.0; 5.0; 6.0; 7.0 and 8.0, with the incubation time of 10 minutes at a temperature of 650 C. Each stage performed three repetitions and analyzed using spectrometry. The results obtained showed the highest protein content in precipitation with ammonium sulfate 60% in the amount of 37.214 mg / ml and the optimum pH of bromelain enzyme activity at pH 7.0 with the value of the activity of 1,081 units / gram.Keywords: ammonium sulfate precipitation, bromelain enzyme, pineapple weevil (Ananas comosus),pH

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