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Identifikasi Molekular dan Morfologis Udang Air Tawar Anggota Genus Macrobrachium Bate, 1868 (Decapoda: Palaemonidae) dari Air Terjun Tegenungan, Gianyar, Bali (In Press)

Nabil, Wildan Ahmad, Eprilurahman, Rury

Biogenesis: Jurnal Ilmiah Biologi Vol 6, No 2 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Macrobrachium is one of prawn genera which has largest species number in family Palaemonidae, with some species of this genus being important commodity in aquaculture. Morphological characters for identification of this genus depend on environmental condition, growth phase, sex, and social dominance. This makes morphological identification on this genus quite difficult and tricky. Alternative approaches are required for better methods of Macrobrachium identification. DNA barcoding using 16S mitochondrial rRNA appears to be one promising method for Macrobrachium identification. This research aims to identify Macrobrachium from Tegenungan Waterfall using morphological and molecular analysis. Samples were taken from 4 sampling sites beneath the waterfall. All specimens were identified using morphological methods according to Holthuis (1950) dan Short (2004). Specimen CR 07a and CR 10 were identified using molecular method. The molecular analysis utilized 16Sar as forward primer and 16Sbr as reverse primer. From 11 specimens, 10 were identified as Macrobrachium. Specimen CR 07a was identified morphologically as M. horstii. According to BLAST analysis, specimen CR 10 was recognized as M. horstii with similarity up to 99% to GenBank specimens (JF310718.1 dan FM986616.1). Specimen CR 07a only reach 97% similariry to both GenBank specimens. Genetic distance analysis between specimen CR 07a and CR 10 assumed high genetic diversity, or even cryptic species indication in M. horstii population of Tegenungan Waterfall.

Deteksi Gen Ketahanan Terhadap Powdery Mildew Pada Melon (Cucumis melo L.) “Aramis” (In Press)

Daryono, Budi Setiadi, Yambise, Hani Christin

Biogenesis: Jurnal Ilmiah Biologi Vol 6, No 2 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Melon (Cucumis melo L.) usualy used as a fresh fruit, which contained high vitamin C and in Indonesia melon is one of the favorite fruit. Indonesia is a country that has a high biodiversity. One of the potential strategies in order to improve productivity and quality of crops competitiveness is through plant breeding. This study used melon Aramis from PT East West Seed Indonesia hybrid melon from crosses between 7319 (F) with 7347 (M). The purpose of this study is to determine the phenotype character of melon cultivar Aramis and to detect powdery mildew resistance gene. The research is conducted in Pusat Inovasi Agro Teknologi (PIAT UGM) Yogyakarta and at the Laboratory of Genetics Faculty of Biology, University of Gadjah Mada. Breeding and maintenance is carried out for 6 weeks until the plant grown to enter the age of the harvest. The harvest is later transferred to the Faculty of Biology Genetics and Breeding Laboratory, resistance genes detection  is conducted  by  Sequence Characterized Amplified Regions method (SCARs) using PCR. The results showed negative result of Aramis cultivar on detection of gene resistance to powdery mildew.

Kapang Kontaminan Pada Ikan Toreh (Hyporhamphus affinis) yang dijual di Pasar Tradisional Kota Ternate Maluku Utara

Nurhasanah, Nurhasanah, Sundari, Sundari

Biogenesis: Jurnal Ilmiah Biologi Vol 6, No 2 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Dried fish from fish Julung (Hyporhamphus affinis) is one type of fish food products that are found in the traditional market of Ternate city. This product is commonly known as fish toreh. The main problem of this product is storage packaging by using an open bamboo set that can be contaminated by various microbes. The purpose of this study was to inventory and identification of contaminant molds isolated from dried fish of H. affinis on a 17-day storage period. The research was conducted in Mathematic and Natural Science laboratory by using dried fish smoke (toreh) from H. affinis obtained from traditional market in Ternate city. Isolation and identification of molds using standard microbiological procedures. Media growth of mold using Potato Dextrose Agar (PDA). Several genus and species of mold found as contaminant mold are Aspergillus spp., Eurotium spp., Torula spp., Cladosporium spp., Polypaecilum pisce and Catenaria fuliginea.

Upaya Pemeliharaan Dengan Frekuensi Pemberian Pakan Udang Kecepe (Acentes japonicus) Berbeda Terhadap Pertumbuhan Mutlak Ikan Lontok (Ophiocara porocephala Valenciennes, 1837) (In Press)

Khairul, Khairul, Hasan, Uswatul

Biogenesis Vol 6, No 2 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

The spangled gudgeon (Ophiocara porocephala Valenciennes,1837) is one of the fish that live in the waters of Belawan and has important economic value to the community. But lately the wild population continues to decline due to factors that supposedly over fishing, waters pollution and habitat degradation. Cultivation effort is one of the solutions to keep the preserved and decrease arrests in nature. The study of associated cultivation efforts need to be made through research. The purpose of this research is to know the frequency of feeding the shrimp kecepe (Acentes japonicus) the right against the growth of absolute spangled gudgeon fish. The benefits of research as the basis for the management and preservation of fish decreased as well as the increasingly Spangled Gudgeon fish efforts do local fish conservation in Belawan Rivers. Results of the study showed the average length of the highest absolute growth was at the treatment D (1.10 cm), followed the treatment C (0.90 cm), treatment B (0.80 cm), and the lowest at the treatment A (0.73 cm). Variansi analysis results indicate that the Fh (8 **) > 0.05 Ft (4.07) means the treatment of natural feeding shrimp kecepe very real influence (highly significant) against the growth of the absolute length of the juvenile spangled gudgeon.

Uji Zat Pengatur Tumbuh Terhadap Setek Lada Perdu (Piper nigrum L.) (In Press)

Wahyudi, Wahyudi, Deviani Duaja, Made, Kartika, Elis

Biogenesis Vol 6, No 2 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Pepper is a commodity of high economic value, but the production is still low due to the use of seeds that are not qualified. The use of seedling from vegetative propagation is the best way to ensure the quality of the seed production. Pepper has been comemercially propaged by rooting of cuttings. To induce roooting in cuttings, use of growth regulators can stimulate root growth. This research was conducted at Teaching and Research Farm, Faculty of Agriculture, Jambi University, in order to find the suitable type of PGR to stimulate pepper root growth. The experimental design used was Completely Randomized Design with one factor plant regulator consisted of seven treatments, Rootone-F 12.5 g /l, coconut water concentration 25%, cow urine concentration 25%, extract of green bean sprouts + 25% moringa leaf extract, 25 % of young corn seed extract + 25% moringa leaf extract, 25% of bamboo shoot extract and 25% of hump banana extract. The observed variables were live crop percentage, percentage of cuttings sprouted, root cutting percentage, shoot length, shoot diameter, root length, dried shoot weight and root dry weight. The results of this study indicate that synthetic plant growth regulators and natural plant growth regulators have a significant effect on the growth of pepper cuttings. The natural plant growth regulator of coconut water with concentration of 25% can increase the growth of pepper cuttings. 

Extract of Dragon Fruit Pulp (Hylocereus polyrhizus) Potentially Stain Chromosomes of Red Onion (Allium ascalonicum) (In press)

Genesiska, Genesiska

Biogenesis Vol 6, No 2 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Side effect of aceto-orcein as a dye of chromosome structures remains some risks. This dye may remain tissue damage on eyes mucous membrane, burnt by skin contact, severe irritation of respiratory by spray mist. Exploration of natural dye is an alternative solution. Betacyanin of red dragon fruits Hylocereus polyrhizus pulp might have a potential role to stain chromosome. Aim of this study is to extraction of dragon fruit pulp (Hylocereus polyrhizus) to stain chromosomes of mitotic cells of red onion Allium ascalonicum. The method used in this research is qualitative description by using squash method with aceto-orcein as synthetic dye and two treatments of extract ratio of red dragon fruits pulps as natural dye. The result revealed that 2:1 ratio of red dragon fruits and distilled water could stain chromosomes of red onion somatic cells than 1: 1 ratio of red dragon fruits and distilled water. Further specific solvent is required to further investigation to get best betacyanin extract of red dragon fruits pulps.

Pemberian Fusarium Non-Patogen dan Tricoderma Untuk Menghambat Penyakit Busuk Pangkal Pada Bawang Putih

Septhiani, Silvia, Oga Nusantari, Diah, Nasir, Deni

Biogenesis: Jurnal Ilmiah Biologi Vol 6, No 2 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

The need for garlic is quite high in Indonesia, but it is not offset by the amount of production. The decrease in production is caused by one of them by a base rot. This study was aim to determine the effect of the use of antagonistic fungi in overcoming base rot in garlic. The antagonistic fungi used are Trichoderma and non-pathogenic Fusarium. This research was conducted using a Completely Randomized Design (CRD) with 3 factors each with 2 treatment levels, resulting in 8 treatment combinations. The results showed that non-pathogenic Fusarium without integrated with other fungi reduced the symptoms of rotten tubers and increased the weight of fresh and weighted tubers. The integration of pathogen and non-pathogen Fusarium, also Trichoderma can reduce the symptoms of base rot.

Inventarisasi Tumbuhan Paku di Jalur Ciwalen Taman Nasional Gunung Gede Pangrango Jawa Barat

Fatahillah, Ibrahim, Fajar Lestari, Indri, Salsabila, Khairunnisa, Pratiwi, Ratna, Amalia, Tasya, Septiyaningsih, Ari, Kulsum, Umi, Hendi Ristanto, Rizhal, Sedayu, Agung

Biogenesis: Jurnal Ilmiah Biologi Vol 6, No 1 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

After being observed as a whole, Gunung Gede Pangrango National Park has a high diversity of Ferns. This research aims to describe Ferns in the Ciwalen Gunung Gede Pangrango National Park, so it is hopefully could describe the diversity of ferns in that path. The Inventory of ferns is done by using exploration method with random sampling technique which then identified with General Key to Malayan Fern referral. The results of this research showed that there are ten types of ferns that have been founded in the Ciwalen Gunung Gede Panggrango National Park, such as Sphaerostephanos sp., Asplenium truncata, Nephrolepis davallioides, Botrychium daucifolium, Selaginella opaca, Huperzia phlegmaria, Equisetum ramosissimum, Didymochlaena truncatula, Hymenophyllum sp., and Diplazium poliferum. Features any distinctive Ferns can be seen from the form such as  rhizome, type of  frond, an accessory, the sorus, habitats that were found, and others.

Variabilitas Genetik Ornithoptera croesus lydius Kupu-Kupu Endemik Pulau Halmahera Berbasis Data Molekuler PCR-RAPD

Masud, Abdu, Abdullah, Abubakar, Roini, Chumidach

Biogenesis: Jurnal Ilmiah Biologi Vol 6, No 1 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Ornithoptera croesus lydius is one of endemic butterlfy in Halmahera island North Maluku. It is macrolepidotera tipe, with interesting colour body at male and female butterfly. At Ornithoptera croesus lydius male and female butterfly have variation morphology size and colour body. The genetic variability on the  O.c lydius  butterflies is level intraspecies variability. The objective of the research is to know the genetic variability on the O.c lydius based on PCR-RAPD marker. The most value of genetic distance  is 0,783 on O.c lydius male and female from hotspot at 780 masl. The percentage of polimorphic  is 71,95%. Base on similarity value and polymorphic percentage show genetic variability on intraspecies level of the Ornithoptera croesus lydius.    

Pergerakkan Darat Ikan Amfibi Andamia heteroptera Bleeker

Nugroho Susanto, Gatot, Ressa utari, Fransisca

Biogenesis: Jurnal Ilmiah Biologi Vol 6, No 1 (2018)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Andamia heteroptera is amphibious fish that walk in land. That behavior used modification fins for terrestrial locomotion. The aim of the research is to know locomotion of A. heteroptera in land and function of fins when used it for locomotion organ. The result shows that A. heteroptera used pectoral fins, pelvic fins, anal fin and caudal fin for locomotion organ. The sucker disk help for the movement. Caudal fin used for base organ when the fish was jump.