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JURNAL PEMULIAAN TANAMAN HUTAN
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PENGARUH KEKERINGAN TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TANAMAN NYAMPLUNG (Callophylum inophyllum L.) DAN JOHAR (Cassia florida Vahl.) DARI PROVENAN YANG BERBEDA

Hidayati, Nur, Hendrati, Rina Laksmi, Triani, Arie, Sudjino, Sudjino

Jurnal Pemuliaan Tanaman Hutan Vol 11, No 2 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

Nyamplung (Callophylum inophyllum L.) and johar (Cassia florida Vahl.) are alternative plants that are resistant to drought. Global warming that occurred in the last few years caused extreme climate change, one of which is drought. The purpose of this research were to examine and determine the growth and development of plants and the response of morphology, physiology, and anatomy of these plants to drought condition, and adaptive species to drought. This research used drought stress treatment for 10 days, 20 days, 30 days, and 40 days, while control plants were watered every 2 days. Each treatment used 3 replications. Parameters observed were plant height, stem diameter, number of leaves, leaf area, proline levels, plant water content, total dry weight, root dry weight, stomata index, root anatomy, anatomy of stem, and leaf anatomy. Physiological data obtained were analyzed using T-test. The results showed that plants responded to drought stress by decreasing plant height, stem diameter, number of leaves, leaf area, root dry weight, total dry weight, water content of plants, transpiration rate, stomata lindex, root cortex thickness, leaf mesophyll thickness, leaf phloem thickness, other wise occurred increasing level of proline, root epidermis thickness, stem xylem diameter, stem cortical thickness and leaf xylem thickness. Johar plant was more adaptive plant to drought stress than nyamplung plant.

HUBUNGAN KEKERABATAN Shorea gysbertsiana DENGAN TIGA JENIS Shorea PENGHASIL TENGKAWANG LAINNYA BERDASARKAN PENANDA RAPD

Sulistyawati, Purnamila, Widyatmoko, Anthonius YPBC

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

The Shorea tengkawang species; Shorea pinanga, S. stenoptera, S. macrophylla, and S. gysbertsiana known for their high-value wood and non-wood products. Shorea tengkawang produces tengkawang oil which usually used as a raw material and supporting material for the manufacture of food, pharmaceuticals and cosmetics. This study determines the genetic relationship of S. gysbertsiana to other three Shorea species uses RAPD (Random Amplified Polymorphism DNA) specific loci. There were 13 specific alleles obtained from 6 RAPD primers. Among these specific alleles, there were 8 loci shared between S. gysbertsiana and S. macrophylla, and 2 loci shared with S. stenoptera. There were no specific loci shared between S. gysbertsiana and S. pinanga. This study found one specific locus for S. gysbertsiana and one specific locus between S. stenoptera and S. pinanga. These results revealed a very close genetic relationship of S. gysbertsiana to S. macrophylla and S. stenoptera.The specific loci found in this study can be used to support the morphological identification, also for supporting conservation program of these four Shorea species.

REGENERASI PERAKARAN PLANTLET IN VITRO DAN EX VITRO PADA KULTUR JARINGAN CENDANA (Santalum album)

Putri, Asri Insiana, Herawan, Toni

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

Cendana (Santalum album) merupakan pohon hemiparasit bernilai tinggi, digunakan secara luas dalam industri farmasi. Sejak1998, spesies ini dinyatakan termasuk kategori rentan oleh IUCN Red List. Perbanyakan pohon cendana sampai saat ini mengalami hambatan karena tidak mampu secara seksual, waktu yang lama dan sifat heterozigot, sementara perbanyakan vegetatif makro cendana belum tersedia. Regenerasi cendana melalui pendekatan in vitro masih terbatas karena sulitnya pengembangan tahap perakaran dan aklimatisasi. Tujuan penelitian ini adalah untuk mengamati regenerasi perakaran plantlet in vitro dan ex vitro pada kultur jaringan cendana. Penelitian ini menggunakan metode kultur jaringan pada tahap perakaran dengan media Ĺ GD yang ditambah IBA 20 mg/l; IAA 0,15 mg/l dan kinetin 0,15 mg/l. Klon cendana A.III.4.14 dan WS28 digunakan sebagai sumber eksplan. Regenerasi perakaran diamati selama 6 bulan in vitro dan 3 bulan ex vitro setelah aklimatisasi di rumah kaca. Hasil penelitian ini menunjukkan bahwa klon A.III.4.14 mempunyai persentase terbentuknya akar primer yang lebih rendah (41,85 %) dibandingkan klon WS28 (60,44 %) dan sebaliknya klon A.III.4.14 mempunyai persentase terbentuknya akar sekunder yang lebih tinggi (58,15 %) dibandingkan klon WS28 (39,56 %). Hemiparasit cendana mempunyai kapasitas untuk melakukan fotosintesa dan dapat melakukan pemanjangan akar, pemanjangan tunas serta pertumbuhan daun secara in vitro tanpa disertai inang. Namun demikian terjadi perubahan bentuk akar ex vitro setelah aklimatisasi, rambut-rambut akar tumbuh pada perakaran ex vitro hanya dari plantlet yang berakar sekunder in vitro. Kondisi ini penting untuk seleksi perakaran in vitro cendana agar diperoleh sistem perakaran yang lebih baik pada aklimatisasi.

SELEKSI DAN PEROLEHAN GENETIK PADA UJI KETURUNAN NYAWAI (Ficus variegata Blume) DI BANTUL

haryjanto, Liliek, Prastyono, Prastyono, Hadiyan, Yayan

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

Nyawai (Ficus variegata Blume) is a fast growing species which is promising for forest industrial plantation. Tree improvement of nyawai was then initiated through some progeny trials involving wide range of genetic base in Bantul. Study on initial growth reported that nyawai originated from Cilacap-Pangandaran showed higher genetic variation than those from other locations. However, further growth performance including the effect of selection in the progeny trial was not reported. This study was aimed to observe the growth and genetic parameter of nyawai in the progeny trial at advanced age. The genetic gain resulted from series of within plot selection was also estimated. The design of progeny trial was a randomized complete block with 19 families from Cilacap-Pangandaran, 4 non-contiguous tree-plot, 7 blocks at spacing of 5 m ◊ 5 m. The observed traits were height, diameter at breast height and volume at four years of age. The results of study showed that survival rate was high at 89%. The mean annual increment for height, diameter and volume were 1.52 m/yr; 2.35 cm/yr and 8.65(◊10-3) m3/yr, respectively. The proportion of variance to the total variance for family, plot and within plot ranged from 0.06% to 2.10%, 25.54% to 27.50% and 70.57% to 73.91%, respectively. In general, narrow-sense heritability for individual, family and within family were low. Genetic gain from within family selection that was practiced as within plot selection using selection ratio 25% were also low ranging from 0.19% to1.91% for all traits.

PENGARUH MIKORIZA ARBUSKULA DAN INANG Portulaca sp. TERHADAP AKLIMATISASI PLANTLET CENDANA (Santalum album L.)

Herawan, Toni, Putri, Asri Insiana

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

Cendana (Santalum album L.) menghasilkan kayu aromatik bernilai ekonomi tinggi yang dibutuhkan pada berbagai industri. Cendana dicanangkan oleh IUCN sebagai pohon kritis terancam punah. Kultur jaringan untuk penyelamatan maupun perbanyakan cendana merupakan teknik yang menjanjikan untuk menurunkan tingkat kelangkaan dan penyediaan bahan baku industri. Permasalahan utama kultur jaringan cendana adalah terhambatnya pertumbuhan perakaran dan tingginya mortalitas planlet pada tahap aklimatisasi. Interaksi akar† hemiparasit cendana dengan biotik tanaman (inang) dan mikroorganisme tanah merupakan penentu aklimatisasi. Tujuan penelitian ini adalah melakukan observasi pengaruh mikoriza arbuskula (5 gram, 7,5 gram dan 10 gram) sebagai bionutrisi dan Portulaca sp. sebagai tanaman inang terhadap aklimatisasi kultur jaringan cendana. Tingkat mortalitas plantlet dan pertambahan tinggi bibit cendana digunakan sebagai parameter untuk 25 ulangan pada masing-masing perlakuan. Hasil penelitian ini menunjukkan bahwa perlakuan 5 gram mikoriza pada plantlet cendana yang ditanam bersama inang Portulaca sp. mempunyai nilai mortalitas terendah (24 %) setelah 12 minggu inkubasi dengan rata-rata tinggi bibit tebaik (6,72 cm Ī 1,33) setelah 16 minggu inkubasi di rumah kaca. Kesesuaian lingkungan biotik pada rizosfer mempunyai peran penting terhadap aklimatisasi plantlet cendana.†

INVENTARISASI DAN IDENTIFIKASI PENYEBAB PENYAKIT PADA Acacia auriculiformis DI YOGYAKARTA

Hidayati, Nur, Hendrati, Rina Laksmi

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

Acacia auriculiformis is a fast growing species mostly planted in marginal lands with less intensive in cultivation. Problems found on A. auriculiformis cultivation include disease attacks which then caused a significant economic reduction on the plantation. The aim of this study is to determine causes, intensity and severity of the diseases attacking A. auriculiformis plants. The research was conducted on two observation plots, in the nursery and in clonal bank area established in Yogyakarta. Genetic materials planted in the plots were collected from clonally propagated of trees selected in second generation progeny trial of A. auriculiformis established in Wonogiri, Central Java. Observations of disease signs and symptoms in the two plots were undertaken with 100% plants inventories in rainy and dry seasons. Postulate Koch was then performed on this study to identify the pathogens. The result showed that the powdery mildew caused by Oidium sp. is a dominant disease attacking 100% A. auriculiformis both in the nursery and on clonal bank areas, occurring not only during the rainy season but also during the dry season. There were also other diseases attacking A. auriculiformis namely black mildew caused by Meliola sp, phomopsis, leaf spot disease caused by Phomopsis sp. and root rot disease caused by Ganoderma steyaertanum.

ISOLASI DAN IDENTIFIKASI PATOGEN PENYEBAB PENYAKIT LODOH PADA SEMAI KALIANDRA (Calliandra callothyrsus)

Hidayati, Nur

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

Procurement of qualified forest tree seeds is one of the determinants of the sustainability of forest management. Pathogen attacked in the nursery is one of the causes of the unfulfilled targets for the provision of seeds of forest crops needed. Therefore, the development of disease in the nursery need to be studied in order to make precautionary or control measures appropriately. The aim of this study is to isolate and identify causes of damping off which causing death in seedling of †Kaliandra (Calliandra callothyrsus).Seedling of Kaliandra which consists of 6 family numbers and come from 2 provenans Papua and NTT shows death due to damping off disease. Seedling of Kaliandra which showed death isolated then isolates were observed macroscopically and microscopically ,Postulat Koch test was done to identify cause of disease causing death at Kaliandra seedling.The results of identification indicate that the causes of damping off disease are Fusarium sp and Rizoctonia sp.†

PARAMETER GENETIK SIFAT PERTUMBUHAN DAN KERAPATAN KAYU KLON Eucalyptus pellita F. Muell. DI DUA TAPAK YANG BERBEDA DI KALIMANTAN TIMUR

Ramadan, Achmad, Indrioko, Sapto, Hardiyanto, Eko Bhakti

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

Industrial forest plantations have an important role in fulfilling the wood demand. Based on global industrial development, the forest plantations industry will increase in the following years. Eucalyptus pellita has become main species in Indonesia forest plantations because it has a short cycle and wood products are suitable to forest industry. The average productivity of E. pellita plantations in Indonesia is still low and high variation. In an effort to increase the productivity, the first step is a better understanding of genetic control on growth and basic density. This study aims to determine the genetic parameters for growth and basic density of E. pellita clones on two different sites. The clonal trials was conducted at two different sites. Both clonal trial are designed using RCBD. The number of clones tested in both trial was 30, 5 blocks and 25 tree/plot. The result of the study showed that the effects of clones vary greatly according the enviromental conditions. The clones- environemntal interaction of growth trait is higher than the basic density. This is in line with genetic parameters of growth trait that are less stable than the basic density. The expected genetic gain of growth trait is higher than the basic density and at the same time there was a weak genetic correlation (there is even a negative) between growth trait and basic density. Therefore carefulness is needed in selecting clones when the two traits are used as selection parameters.

PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)

Nurtjahjaningsih, I. L. G., Herawan, Toni, Rachma, Reza Permatasari, Rimbawanto, Anto

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.

INTERAKSI FAMILI √? LOKASI PADA UJI KETURUNAN GENERASI KEDUA Eucalyptus pellita

Mangkuwibowo, Fasis, Indrioko, Sapto, Nirsatmanto, Arif

Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

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Abstract

The interaction assessment of genotype and environment is necessary to find out an effective selection strategy in progeny trial, particularly in advanced generations. This study aims to observe the effect of family √? site interaction in second generation progeny trial of Eucalyptus pellita planted at two locations: Wonogiri, Central Java and Pelaihari, South Kalimantan. The trials design were completely randomized block design (CRBD) with 49 families, 6-10 blocks, 5 tree-plot and 4 m √? 1.5 m spacing. Data measurements included height, diameter at breast height, and stem volume at two and four years age. The study results showed that the families had significant differences (p<0.01) for all measured traits at two and four years age, but the family and site interaction was not statistically significant. Family heritability across the two sites were moderate to high for all traits with the type B genetic correlation ranged from 0.67 to 0.94. Heritability and genetic correlation tended to be higher as tree getting older. Less than 16% of families showed an interactive rank changes in the two locations based on multiple-traits selection index. In general, family selection by combining data from the two sites of progeny trial provided higher genetic gain than those by indirect selection, but it was varied to those direct selection on each site. Therefore, these results imply that, the strategy for family selection by combining data from the two sites of progeny trials should be practiced in appropriate combination and direction by considering the magnitude of genetic variation.

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