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Microbiology Indonesia
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292
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Helianti, Is ( 1. BPPT 2. Indonesian Society for Microbiology )

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

Physiological Profiling and Microorganism Community Analysis of Cirebon Shrimp Paste Fermentation “Terasi” using BIOLOGTM EcoPlate

ASTUTI, DEA INDRIANI, TAUFIK, INTAN, ACHNAFANI, DINI, PRISCILA, EZRA SUCI

Microbiology Indonesia Vol 1, No 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

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Abstract

Terasi or shrimp paste is an Indonesian traditional seasoning made from fermented small shrimp or krill. Different indigenous microorganism community exhibit different physiological function due to lack standard in its materials and processing. This study aimed to determine physiological profiles and microorganism community in Cirebon shrimp paste fermentation. BIOLOGTM EcoPlate was used to obtain microbial physiological function of the krill and 2-months old shrimp paste. Microorganisms were later isolated from EcoPlate substrate to determine its community structure. Average Well Color Development (AWCD) from krill was thirty times higher than shrimp paste. Interestingly, this study revealed a shift of carbon source utilization at day-28 of fermentation from amino acid and polymer to phenolic compound. In addition, AWCD index increased in accordance with increased of microorganism community complexity at day-28. Within 56 days of fermentation there was a slightly increase in water, fat, and carbohydrate content. In contrast, there was decrease in protein, ash content, and acidity level from neutral to acid, with salinity level resulted in between 16.26% to 21.42%. We conclude that there is a change of microorganism community within shrimp paste fermentation corresponding to metabolism activity which affects the product quality. 

THE EFFECT OF CARBON AND NITROGEN SUPPLEMENTATION ON BACTERIOCIN PRODUCTION OF LACTIC ACID BACTERIA FROM PICKLED YELLOW BAMBOO SHOOTS (Dendrocalamus asper)

HARTAJANIE, LAKSMI ( Fakultas Teknologi Pertanian UNIKA Soegijapranata Semarang ) , LINDAYANI, LINDAYANI ( Fakultas Teknologi Pertanian UNIKA Soegijapranata Semarang ) , SANTOSO, LORENTIA ( Fakultas Teknologi Pertanian UNIKA Soegijapranata Semarang )

Microbiology Indonesia Vol 1, No 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

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Abstract

Six selected lactic acid bacteria (LAB) isolates from pickled Yellow Betung bamboo shoots were grown in de Mann Rogosa Sharpe-Broth (MRSB) media with different supplementation combination. The cell-free supernatant were evaluated for their ability to produce bacteriocin  by adjusting its pH to 6.0 in order to remove organic acid effects. The bacteriocin activity was  assayed by agar-well diffusion method. The inhibitory activity calculated in Activity Unit (AU in mm2 mL-1) of bacteriocins. The aims of this paper is to explore the effect of different medium compositions on bacteriocin production and its inhibitory activity against pathogenic bacteria (Listeria monocytogenes FNCC 0156, Staphylococcus aureus FNCC 0047, and Escherichia coli FNCC 0091).Supplementations of carbon and nitrogen sources induced production of bacteriocins. LAB isolates grown in media without supplementation could not produce bacteriocins.  Growth of isolate D44 in the presence of 2% of glucose and 2% of yeast extract yielded the largest bacteriocin inhibitory activity levels of 3179 AU mL-1 against Listeria monocytogenes FNCC 0156, 4663 AU mL-1 against Staphylococcus aureus FNCC 0047, and 3109 AU mL-1 against Escherichia coli FNCC 0091.

Bacterial leaching of an Indonesian complex copper sulfide ore using an iron-oxidizing indigenous bacterium

CHAERUN, SITI KHODIJAH, PUTRI, FRIDENI YUSHANDIANA, MINWAL, WAHYUDIN PRAWITA, ICHLAS, ZELA TANLEGA, MUBAROK, MOHAMMAD ZAKI

Microbiology Indonesia Vol 1, No 1 (2018): March 2018
Publisher : Indonesian Society for microbiology

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Abstract

The bioleaching of an Indonesian complex copper sulfide ore was studied in shake flasks over a period of 14 days using an iron-oxidizing indigenous bacterium at room temperature (28 oC) and various pulp densities (5% and 20%). The bioleaching suspensions were periodically analyzed for Cu and Fe concentrations as well as Eh, pH and DO values. Cu bioleaching efficiencies at 5% pulp density were higher than those at 20% pulp density, which correlated with Fe concentration in solution. Over a period of 14 days, the pH of bioleaching suspension was in the range of 5 ~ 9, indicating that Cu bioleaching was greatly influenced not only by proton H+ dan ferric ion but also by extracellular polymeric substances (EPS) generated by the bacterium. The current study may improve our better understanding on the bacterial action for bioleaching of complex copper sulfide ores that remains debated so far as refractory ores.

Potential Degradation of SARA (Saturated, Aromatics, Resinics, Asphaltenes) Fractions of Crude Oil by Reservoir Indigenous Bacteria from South Sumatera

ASTUTI, DEA INDRIANI, PURWASENA, ISTY ADHITYA, ADITIAWATI, PINGKAN, SANI, INDIANI, ARIADJI, TUTUKA, ABQORY, M. H.

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

MEOR (Microbial Enhanced Oil Recovery) technology utilizes metabolic activity of microorganisms such as degradation of hydrocarbon fractions which alters oil characteristics to facilitate and increase oil recovery from reservoir. This research focused on isolation of indigenous hydrocarbonoclastic bacteria that were capable of degrading SARA (Saturated, Aromatic, Resinic, Asphaltenes) fractions of crude oil to be utilized in MEOR. Sequential isolation of hydrocarbonoclastic bacteria were conducted using Nutrient Broth and Stone Mineral oSalt Solution medium supplemented with 2% (v/v) crude oil and 0.1% (w/v) yeast extract and incubated at 50C, 120 rpm agitation. Isolates retrieved were screened based on its activity to degrade crude oil, indicated by resazurin assay result. Physical and chemical characteristics of crude oil altered by selected isolates were observed using column chromatography, biometric test, GC-MS analysis, IFT and viscosity measurements. Isolates with the best degradation activity were identified with 16S rRNA gene sequencing. Among thirty-one bacterial isolates obtained from sequential isolation, six isolates exhibited high oil degrading ability. SARA assay showed degradation activity of those isolates to each of SARA fraction were around 6-70%.  This degradation was followed by significant CO production ranging from 2000-4000 mg (value of p<0.05). Microbial 2 degradation activity exhibited changes in chemical and physical characteristics of hydrocarbons showed by changes in composition of SARA fraction, decreased viscosity and IFT of crude oil 17-31%. This research identified three isolates with the best hydrocarbon fraction degrading ability were identified as different strain of Bacillus licheniformis and confirmed their high potential to be utilized in MEOR technology.

Isolation and Identification of Ethanol and Glucose Tolerance Yeasts Strain from Tacca leontopetaloides

UTAMA, GEMILANG LARA, SUGANDI, WAHYU K., LEMBONG, ELAZMANAWATI, SURYADI, EDY

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

The research aimed to isolate and characterize indigenous yeast strain from Tacca leontopetaloides with respect to the ethanol and glucose tolerance ability. Research done experimentally and the data analyzed descriptively. Yeasts isolated from 1g Tacca leontopetaloides grown at modified Potato Dextrose Agar/PDA (Oxoid Ltd.) with 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin addition. Yeasts-like colony was tested in the ability to tolerate ethanol and glucose contents by grown on modified Nutrient Broth/NB (Oxoid Ltd.) with 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin then added with glucose monohydrate (10%, 20%, 30%) or ethanol (10%, 20%, 30%) and incubated for 72h at ambient (23-28°C). Optical density (OD) was read for UV absorbance at 600 nm using UV-Vis spectrophotometer every 24h until 72h. The strain of best isolate with the ability to tolerate high ethanol and glucose contents were identified by the sequence analysis of ITS (Internal Transcribed Spacer) region using primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′). The sequencing was performed at Macrogen Inc. (Seoul, South Korea), and the sequences was compared with the GenBank database using BLAST (Basic Local Alignment Search Tools) algorithm (http//:www.ncbi.nlm.nih.gov/BLAST/). Results shown there are four yeasts-like isolate and the TK1 isolate showed the best ethanol tolerance ability with highest OD at 30% ethanol concentration (0.486) and the highest OD at 30% glucose concentration (1.732). The species identification identified the TK1 isolate as 99% identical with Candida natalensis (ITS1) and 100% identical with Candida quercitrusa (ITS4).

Molecular Identification of Thermally-tolerant Symbiotic Dinoflagellates from Hard Coral (Scleractinia) in Biawak Island, Indonesia

RORIRIS, EVINA TAMI, AGUNG, MOCHAMAD UNTUNG KURNIA, ASTUTY, SRI, MULYANI, YENI

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

Symbiodinium is phototrophic dinoflagellates that occur as endosymbionts in various marine invertebrates and protists, forming mutualistic symbiosis with their hosts. The composition of Symbiodinium populations may also play an important role in the tolerance or sensivity of corals towards bleaching. Therefore, this research aims to identify thermally-tolerant Symbiodinium of scleractinian corals in Biawak Island using molecular techniques. Sampling was carried out from Acropora sp and Porites sp, from the depth of 3-5 meters. Symbiodinium was TMisolated through metagenomic approach using ZR Soil Microbe DNA Kit and 28S nrDNA gene was amplified TMusing Polymerase Chain Reaction (PCR). The resulted sequences were processed using BioEdit software and TMMEGA 5.2 for phylogenetic tree construction. The results indicated that isolates of ZX-ACP-ST.1 and ZXPORITES-ST.3 have similiarity to registered sequences of Symbiodinium thermophilum with identity value of TM99% and 98%. BLAST analysis of ZX-PORITES-ST.2 isolate indicated high identity (99%) to sequences of Symbiodinium sp. clade C while ZX-PORITES-ST.3 isolate has 99% similiarity to Symbiodinium sp. CG8. Phylogenetic analysis using UGPMA method showed that all isolates had a very close relationship and thought to have come from Symbiodinium clade C.

ITA REGISTRATION FORM AND BACK COVER

Helianti, Is ( 1. BPPT 2. Indonesian Society for Microbiology )

Microbiology Indonesia Vol 11, No 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

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Abstract

ITA REGISTRATION FORM AND BACK COVER

Helianti, Is ( 1. BPPT 2. Indonesian Society for Microbiology )

Microbiology Indonesia Vol 11, No 2 (2017): Juni 2017
Publisher : Indonesian Society for microbiology

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Abstract

The Utilization of Modified Cassava Flour (Mocaf) Industry Waste and Peat as Carrier of Nitrogen-Fixing Bacteria and Phosphate Solubilizing Bacteria Inoculant

ROSARIASTUTI, RETNO ( Sebelas Maret University ) , SUMANI, SUMANI, SUPRIYADI, SUPRIYADI, NURSETYAWAN, MUHAMMAD ARDIAN, DANISWARA, PRAMUSITA YOGA

Microbiology Indonesia Vol 11, No 4 (2017): December 2017
Publisher : Indonesian Society for microbiology

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Abstract

Fertilizer demand in Indonesia increase for increasing agricultural production.Biofertilizer is organic fertilizer with the addition of specific microorganisms which are expected to reduce the amount of inorganic fertilizer. Carrier material plays an important role in maintaining the viability and storage period. Peat is used as a biofertilizer carrier for  a long time. Solid waste of Modified Cassava Flour (Mocaf) which is the remainder of mocaf industry has great potential as a carrier material of good biofertilizers,  because of its nutrient content. The aim of this study was  determining the potential of mocaf solid waste and its combinations with peat as the carrier in supporting the growth of Nitrogen-fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) during the incubation  of microorganisms. The experiment was conducted at the Laboratory of Soil Biology and Biotechnology, Faculty of Agriculture of Sebelas Maret University (UNS) using completely randomized design (CRD) with two factors of a carrier and incubation time as the experimental design. There  were  three  types of  carrier  which  have different  combination. The base material were solid  waste  of  Mocaf  industry   and  peat.  All materials  of carrier  were  mixed and sterilized, than inoculated by Nitrogen- Fixing  Bacteria (NFB)  and  Phosphate Solubilizing  Bacteria (PSB) and incubated  for  60  days. The  growth of  bacterias were analyzed  every 15  days  and  the  chemical composition  of  carrier  were  analyzed  at  the begin and the  end of  research   (incubation).    The results indicated that the incubation time significantlyaffected viability of NFB and PSB. Until 60th day incubation time, still showed the increasing growth of NFB and PSB.