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Majalah Kesehatan Pharmamedika
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Articles 9 Documents
Search results for , issue " Vol 3 N0 1 2011" : 9 Documents clear
Defisiensi Antibodi Primer dan Hubungannya dengan Kelainan Kulit Magdalena, Maria; Mappiasse, Alwi
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Primary antibody deficiency is the most abnormality found in primary immunodeficiency. It is caused by function defect and cell B development which antibody production. it is commonly found on baby and children, except common variable immunodeficiency (CVID) which mostly on adult. Cutaneous manifestaion caused by primary antibody deficiency, not specific, and not as many as other defeciency like cell T deficiency. Cutaneous abnormality caused by bacterial infection is the main problem beside atopy and autoimmune disease. Immunoglobulin replacement therapy has a important role in primer antibody deficiency management
Formulasi Krim Ekstrak Etanol Daun Kirinyuh (Euphatorium odoratum. L) untuk Penyembuhan Luka Yenti, Revi; Afrianti, Ria; Afriani, Linda
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Formulation of cream for wound healing has been studied. Cream formula consisting of 2.5%, 5% and 10% ethanolic extract of kirinyuh leaf (Euphatorium odoratum L.) as an active ingredient. The formulas were evaluated for their organoleptic, homogeneity, cream type, pH, washability, stability of temperature, particle size distribution, skin irritation, and effect wound healing in mice white male with Mortons method. The evaluation formulation results showed physicaly stable and provide a healing effect on wound. The results showed that the formula cream with a concentration of 10% at 12th day, with 100% percentage of wound healing.
Aktivitas Sitotoksik Ekstrak Etanolik Rimpang Temu Kunci (Boesenbergia pandurata) Terhadap Sel Kanker Serviks HeLa dan Sel Kanker Kolon WiDr Handoko, Fransiscus Feby; Maruti, Astrid Ayu; Rivanti, Erlina; Putri, Dyaningtyas Dewi Pamungkas; Meiyanto, Edy
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Temu kunci (Boesenbergia pandurata) rhizome showed cytotoxic effect against T47D breast cancer cell line. It contains Panduratin, a chalcone compund, that has been investigated as chemopreventive agent. The exploration of extract of temu kuci as chemopreventive agent was expected to be an alternative for cancer therapy. The aim of this research was to determine the cytotoxic activities of ethanolic extract of temu kunci against HeLa cervix cancer and WiDr colon cancer cell line. The cytotoxic activities of ethanolic extract of temu kunci were tested using MTT assay against HeLa and WiDr cells. The IC50 values were obtained using linier regression equation. The ethanolic extract of temu kunci showed cytotoxic activities on HeLa cervix cancer and WiDr colon cancer cell lines with IC50 at 87 µg/mL and 76 µg/mL, respectively. Low IC50 values (<100 µg/mL) showed that ethanolic extract of temu kunci is potential to be developed as chemoprevention agent on cervix cancer and colon cancer. However, its molecular mecahanism need to be explored.
Perbandingan Efektivitas Beberapa Pelarut Terhadap Kelarutan Cerumen Obturans Secara In Vitro -, Syahrijuita; Rahardjo, Sutji Pratiwi; Djufri, Nani . I.; Djamin, Riskiana
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Cerumen obturans is a pahtological condition with no harmfull to the patients but can caused ear tumbness sensation, earache, hearing impairment, deafness and decreasing the quality of life.The objections of the study are to compare the effectivity of six solvents, that are aquadest, NaCl 0,9%, coconut oil, olive oil, carboglycerin 10% and sodium docusate 0,5% againts cerumen obturans by means of in vitro study and to know the most effective duration contact of a solvent to cerumen osmolarity. The study is a laboratory experimental by using 30 specimen of solid cerumen with weight of 40 mg for each. The cerumen osmolarity is established by Spectronic 21 spectrophotometer. The effectivity comparison of solvents are tested with One Way Anova with alfa < 0,05.The effectivity of solvents have a significant differentiation especially in  20th , 25th and 30th minutes. The spectrophotometer that used to established the osmolarity of cerumen have revelead a significant results only in aquadest and NaCl 0,9% againts coconut oil dan olive oil,  The effective duration of contact by in vitro study is ≥ 20 minutes and tends to increased to 30 minutes. In 20th and 25th minutes, NaCl 0,9 % is the most effective solvent, while aquadest is most effective in 30th minutes. Olive oil and coconut oil are less effective solvents. Water-based solvents are more effective than lipid-based solvents.
The Study on Apoptosis of The Liver Cells White Rats Due to the Different Exposure Times and Dosages of Aflatoxin B1 -, Yanwirasti
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Apoptosis is known to maintenance of tissue homeostasis and elimination of cancer cells. Whithout programed cell death, cell proliferation would lack on important component of control of oncogenic process. Apoptosis is caused by various inducers such as chemival compound and toxin. Aflatoxin B1 is a potent toxin. In the liver, it undergoes biotransformation which produces reactive oxigen species, causes cellular stress that initiates apoptosis. However, the correlation between exposure of AFB and the evidence of apoptosis in the liver and development of Hepato Cell Carcinoma has not been elucidated For this purpose, we used an animal experiment with 96 white rats (Rattus Norvegicus). Adult healthy white rats were divided into four groups of 24 rats each, based on the dosages of AFB1 given. Each group was divided further into three subgroups of eight rats based on the length of exposure time to AFB1 . Four dosages of AFB1 , were introduced orally everyday into different groups, consisted of 0, 10, 15 and 20 µg dissolved in 0,2 ml propylene glycol. Three subgroups received the dosage for 12 weeks, 16 weeks, and 20 weeks. At the end of the experiment, the rats were sacrificed. Liver cells with apoptosis were scrutinized using peroxidase insitu apoptosis detection kit and liver cell damages were examined using histological slices stained by haematoxillin cosin. In our analysis, we found that apoptosis of the liver increased until the formation of dysplacia of the liver cells. After that apoptosis decreased. It means that the highest dosages and the longest time exposure AFB1 inhibited apoptosis.  We concluded that apoptosis of the liver cells due to AFB1 caused not only by the damaged of mitochondria (caused by reactive oxygen species) but also by the mutation of p53 which we could see dysplacia of the liver cell in histogical slices.
Pengaruh Pemberian Infusa Tumbuhan Sarang Semut (Hydnophytum formicarum) Terhadap Gambaran Histologi Pankreas Pada Tikus (Rattus norvegicus) Diabetes Terinduksi Aloksan Jeli, Mutiana Muspita; Makiyah, SN. Nurul
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Oxidative stress has been implicated in pathogenesis of diabetes mellitus. In addition to increased production of free radicals, antioxidant defense systems are disturbed in diabetes mellitus. As a result, oxidative stress may cause oxidative damage of B-cell pancreas. Hydnophytum formicarum consists of flavonoid which function as an antioxidant. So that, it necessary do research to know influences of H. formicarum infusion to pancreas histology image of alloxan-induced-diabetic. This research is an experimental with in vivo design. Research subjects were 30 male rats Sprague Dawley strain, in 3-4 months, weight ± 200 gram were divided into 6 groups : normal control, negative control, glibenklamid dosage 0,5 mg/kgBW, H. formicarum dosage 1,26 g/kgBW, H. formicarum dosage 2,52 g/kgBW, and H. formicarum dosage 5,04 g/kgBW. Rats were inducted by alloxan dosage 130 mg/kgBW. Glibenklamid and infusion of H. formicarum were given once per day for 14 days orally. On the 15th day all the rats were then killed to carry out the pancreas. The analysis data using oneway anova test and post hoc test (Tukey).The result revealed that giving of glibenclamide, infusion of H. formicarum dosage 1,26 g/kgBW, dosage 2,52 g/kgBW, and dosage 5,04 g/kgBW was able to increase the size of Langerhans islet and amount of β-cells. Based on that, the conlusion is infusion of H. formicarum could minimize the damage of pacreas of alloxan-induced diabetic image.
Aktivitas Antiproliferasi Ekstrak Etanolik Herba Ciplukan (Physalis angulata L.) Terhadap Sel Hepar Tikus Betina Galur Sprague Dawley Terinduksi 7,12-Dimetilbenz[a]antrasena Fauzi, Ilham Agusta; Amalia, Fikri; Sabila, Nurma; Hermawan, Adam; Ikawati, Muthi; Meiyanto, Edy
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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One of the natural materials as potentially efficacious chemopreventive agents are Ciplukan (Physalis angulata L.). Several previous studies reported that Physalis angulata L. herbs ethanolic extract (PEE) has cytotoxic activity and induction of apoptosis in breast cancer cells MCF-7 and HeLa cervical cancer cells. This study aims to determine the effects of  PEE as an chemopreventive agent on rat liver cells induced 7,12-dimetilbenz[a]anthracene (DMBA). This study used Sprague Dawley strain female rats aged 40-50 days were divided into 5 groups : (1) DMBA control group, mice were induced with DMBA in per oral dose of 20 mg/kg; (2) DMBA + PEE dose 750 mg/kgBW group ; (3) DMBA + PEE dose 1500 mg/kgBW group ; (4) solvent control group of CMC-Na 0,5%; (5) PEE dose 1500 mg/kgBW control group. PEE was dissolved in CMC-Na 0,5% and administered daily, starting the seventh week after administration of DMBA. At the beginning of  the tenth week of the study, rats were necropted and liver organs were isolated and stored in buffered formalin 4%. Qualitative analysis to determine the histopathology of liver cells through staining method of Hematoxyllin & Eosin (HE), while quantitative analysis to determine the level of liver cell proliferation by AgNOR staining method. The results showed in the DMBA control group that liver cell morphology changes that hiperproliferation leading to carcinogenesis. In DMBA + PEE dose of  1500 mg/kgBW group improved the situation of DMBA-induced liver cells histopathology and antiproliferation activity better than DMBA + PEE dose of 750 mg/kgBW on DMBA-induced rat liver cells. The results showed that Physalis angulata L. herbs ethanolic extract can inhibit cell proliferation in rat liver caused by DMBA administration through antiproliferation mechanism and have potential for the development as chemoprevention material on liver cancer
Mekanisme Apoptosis Pada Regresi Sel Luteal Hadi, Restu Syamsul
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Corpus luteum is a transient endocrine gland and its presence plays pivotal role in ovulation process, implantation, and luteinization. The regression of corpus luteum or luteolysis is actually needed for next cycle survival and playing a role in providing new follicles.  Luteal regression represents a broad definition of the process of demise of the corpus luteum that is capable of accommodating all new knowledge evolved on the molecular mechanisms activated or inhibited during the process of regression of the corpus luteum. Apoptosis is controlled by a number of regulator genes such as bcl-2 family. The ratio of Bax expression to Bcl-2 is a pivotal factor for a cell to survive or apoptosis. The increasing Bcl-2 expression will lengthen cell life, whereas increasing of Bax expression promotes cell death.
Uji Toksisitas Akut Ekstrak Etanol Sarang Semut (Myrmecodia pendens) Kalimantan pada Mencit (Mus musculus) Swiss Pandia, Dhayu Mart Hindrasyah; Wibriansyah, Akbar; Pratiwi, Widya; Priadinata, Ahmad Fajrin; Sari, Wening
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
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Sarang semut (Myrmecodia pendans) is an Indonesian native plant that has been used in folk medicine. Researches showed sarang semut has antioxidant effect,  xantinoxidase inhibitor potency, and anticancer activity. The efficacy and safety of this plant should have been proven before used in modern medicine. The objective of this research was to assess the potency of acute toxicity, evaluate toxic clinical symptoms and histopahtologic examination. The research was completly randomized design. The treated animal was 20 Swiss male mice devided into 5 groups; 1 control group with administration of 1% NaCMC  and 4 experimental groups with dosage administration of ethanol extract of sarang semut were 0,1, 1, 10 and 100g/Kg of body weight respectively. Evaluation of the toxic clinical symptoms and death was done in 24 hours and 14 day for survival mice. The test resulted LD50 of ethanol extract of sarang semut was 3,162g/Kg of body weight. All mice in 10 and 100g/kg of body weight groups were death in one hour administration of extract and showed toxic clinical symptom such as hyperactive and convulsion. Histopathologic examination on kidney of the death mice was showed infiltration of inflammation cells and congestive tubules diffuse . The ethanol extract of sarang semut was a slighly toxic substance for Swiss male mice based on Hodge dan Sterner criteria.

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