Bulletin of Health Studies
Articles
5
Articles
SUB-CHRONIC TOXICITY TEST KEMBANG SUNGSANG LEAF EXTRACT (Gloriosa superba L.) ON RENAL FUNCTION OF WHITE RATS

Winarno, M. Wien, Sundari, Dian

Bulletin of Health Studies Vol. 38 No. 4 December 2010
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Abstract

Kembang sungsang (Gloriosa superba L.) including families Colchicaceae, empirically this plant can be used to treat gout, diuretic, rheumatic and cultivated because of its use. All parts of this plant contain alkaloids called colchichin and gloriosin and most found on the tuber. From the results of previous research, the ethanol extract of leaves and flowers antihiperurisemia Breech is included in the class materials "Practically Non-Toxic." However, repeated administration may cause toxic effects on body organs.  Test the effect of 70% ethanol extract of  kembang sungsang leaves of kidney function in male and female white rats. There are 3 (three) doses of test materials which are: 32.25 mg, 21.5 mg and 14.35 mg/100 g bw., And as a control was distilled water. Test and control material was administered orally in accordance with a predetermined time is 45 days, 90 days and 104 days (recovery period for the largest dose) administration. Observation is done by sacrificing mice according to given deadlines. Results of blood biochemistry analysis showed that drug therapy for 45 days, to increase blood urea and creatinine, but the award of 90 and 104 days (recovery) decreased creatinine levels.Keywords: Toxicity, Kembang Sungsang, Gloriosa superba L. Kidney function

WHY BIOSECURITY IS IMPORTANT IN THE INFECTIOUS DISEASE LABORATORIES ?

Halim, Frans X Suharyanto

Bulletin of Health Studies Vol. 38 No. 4 December 2010
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Laboratory biosecurity is the protection, control and accountability forvaluable biological material ( VBM ) laboratories, in order to prevent their unauthorizedaccess, loss, theft, misuse, diversion or intentional release. The efforts of biosecurityhave capability to anticipate the potential probability of releasing biohazard agent fromthe laboratory, the risk assessment study in the infectious disesase laboratories was aneffort to know whether biosecurity measures were applied in the laboratory. The usageof modified checklist questionnaire of biosecurity for collecting data and observationwas done to identify potential hazard in the infectious disease laboratories according tothe conceptional framework of agent, host and environmental principal. The places ofthis assessment are in the five regional referral infectious disease laboratories , i.e.,Universitas Islam Sumatera Utara (UISU) Medan, Universitas Indonesia (UI) - Jakarta,Balai Pengembangan Laboratorium Kesehatan (BPLK) - Bandung, UniversitasDiponegoro (UNDIP) - Semarang , Universitas Hasanudin (UNHAS) – Makassar, onereferral hospital , i.e., Rumah sakit Umum Daerah (RSUD) – Tangerang and onenational referral laboratory of Center for Biomedical and Pharmaceutical Reseach andDevelopment, National Institute of Health Research and Development ( NIHRD),Ministry of Health (MOH), Jakarta. The risk assessment study was done in year 2008-2009. Physical security, personnel management and information security as componentsof biosecurity were not applied properly in the 7 infectious disease laboratories. Applyingbiosecurity in the infectious disease laboratories was very important and need to be donecompletely to anticipate their unauthorized access, loss, theft, misuse, diversion orintentional release.Keywords : biosecurity, bioterrorism , infectious disease laboratory, and valuablebiological materials (VBM)

COMMUNITY COMPLIANCE TO FILARIAL MASS DRUG ADMINISTRATION IN BELITUNG TIMUR REGENCY 2008

., Santoso, Saikhu, Akhmad, Taviv, Yulian, R.D, Yuliani, Rika, Mayasari, ., Supardi

Bulletin of Health Studies Vol. 38 No. 4 December 2010
Publisher : Bulletin of Health Studies

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Mass Drug Administration (MDA) was conducted in Belitung Timur Regencyfrom 2006 to 2007. The research was conducted to evaluation the MDA program I 2008.The number of people examined from 4 village were 2.064 people and out of this number3 people were mf positive with the Mf rate was 0.15%. Compare with the result beforethis study, we find out that there are descent Mf rate from 2.52% in 2005 (before MDA) to0.15% in 2008 (after MDA). The coverage of MDA in 2006 were 97.58% and decrease to95.44% in 2007. The knowledge, attitude and perception of the respondents weregenerally moderate toward filarial transmission, but there are 17 respondents (4.4%)from 385 respondents was not receiving filarial drug when the MDA was going on. Thereare 14 respondents (3.6%) didn’t take the drug, the reason of respondents were: didn’tsick, confused, and fever.Keyword: MDA, coverage, filariasis, compliance, Mf rate.Frans X Suharyanto HalimPuslitbang Biomedis dan Farmasi JakartaWHY BIOSECURITY IS IMPORTANTIN THE INFECTIOUS DISEASE LABORATORIES ?Abstract . Laboratory biosecurity is the protection, control and accountability forvaluable biological material ( VBM ) laboratories, in order to prevent their unauthorizedaccess, loss, theft, misuse, diversion or intentional release. The efforts of biosecurityhave capability to anticipate the potential probability of releasing biohazard agent fromthe laboratory, the risk assessment study in the infectious disesase laboratories was aneffort to know whether biosecurity measures were applied in the laboratory. The usageof modified checklist questionnaire of biosecurity for collecting data and observationwas done to identify potential hazard in the infectious disease laboratories according tothe conceptional framework of agent, host and environmental principal. The places ofthis assessment are in the five regional referral infectious disease laboratories , i.e.,Universitas Islam Sumatera Utara (UISU) Medan, Universitas Indonesia (UI) - Jakarta,Balai Pengembangan Laboratorium Kesehatan (BPLK) - Bandung, UniversitasDiponegoro (UNDIP) - Semarang , Universitas Hasanudin (UNHAS) – Makassar, onereferral hospital , i.e., Rumah sakit Umum Daerah (RSUD) – Tangerang and onenational referral laboratory of Center for Biomedical and Pharmaceutical Reseach andDevelopment, National Institute of Health Research and Development ( NIHRD),Ministry of Health (MOH), Jakarta. The risk assessment study was done in year 2008-2009. Physical security, personnel management and information security as componentsof biosecurity were not applied properly in the 7 infectious disease laboratories. Applyingbiosecurity in the infectious disease laboratories was very important and need to be donecompletely to anticipate their unauthorized access, loss, theft, misuse, diversion orintentional release.Keywords : biosecurity, bioterrorism , infectious disease laboratory, and valuablebiological materials (VBM)

APPLICATION OF IKAN CUPANG BY JUMANTIK FOR DHF DISEASE CONTROL IN PALEMBANG

Taviv, Yulian, Saikhu, Akhmad, Sitorus, Hotnida

Bulletin of Health Studies Vol. 38 No. 4 December 2010
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Palembang city has the highest dengue cases compare to other region in SouthSumatera Province. This study compares two regions, first region with jumantik (kader)and application of ikan cupang, and the second region only applied with jumantik. Thisstudy aims to find public acceptance of ikan cupang (Ctenops vittatus) as larvae controlin the community and to determine larvae index (house index, container index, breteauindex) in both regions. Larvae found in this study were Aedes aegypty, Ae. albopictus andCulex spp. Comparative indicators of mosquito larvae inspection before and aftertreatment in Kelurahan Kebun Bunga were angka bebas jentik from 48% to 86%, houseindex from 52% to 14%, container index from 48% to 25%, and breteau index from 66%to 50%, while in Kelurahan Sukarami angka bebas jentik from 58% to 72%, house indexfrom 42% to 28%, container index from 36% to 50%, and breteau index from 66% to50%. Kelurahan Kebun Bunga and Kelurahan Sukarami categorized as region with highrisk of dengue infection. Ikan cupang was effective as Aedes larvae predator, and moreacceptable to the community compare to the chemical insecticide. This study suggest tomaintain jumantik in order to minimize dengue cases in Palembang city.Key words : dengue fever, jumantik plus, ikan cupang, larvae index.

PRELIMINARY MAPPING STUDY OF Mycobacterium tuberculosis COMPLEX DNA BY Spoligotyping ON ISOLATED SPUTUM OF TUBERCULOSIS PATIENT FROM 10 PROVINCE CAPITAL CITY

Lisdawati, Vivi, Parwati, Ida, Sudarmono, Pratiwi, Sudiro, T. Mirawati, Ramadhany, Ririn, Puspandari, Nelly, Rif’ati, Lutfah, ., Triyani S.

Bulletin of Health Studies Vol. 38 No. 4 December 2010
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Mapping TB genotype of Mycobacterium tuberculosis (Mtb) is an importantstudy to identify their distribution or characteristic and also may lead to improve controlof the disease. This study conducted a preliminary mapping of the tubercle bacilli whichhad been circulating in Indonesia. Cultures of DNA isolated from TB patients at urbanareas in 16 provinces in Indonesia, are chosen based on TB Case Detection Rate (CDR)2006 from Indonesia Directorate General of Communicable Disease Control andEnvironment Health (Ministry of Health), were analyzed by spoligotyping for straindifferentiation. In this first part, the analyzed result only came from urban areas in 10provinces, i.e. Palembang, Bandar Lampung, Serang, Jakarta, Bandung, Surabaya,Banjarmasin, Makassar, Pontianak and Ambon.Sample were 269 DNA from 294 isolates collected from sputum of suspect TB patientswith sputum-smear positive (SS+) and age above 15 years old. All samples were obtainedfrom peripheral health laboratory in each province. The procedure collection isaccordance to Indonesia DOTs guidelines (AFB smears) and samples were transportedfrom those 10 areas to Bacteriology Laboratory at CBPRD. Sputum was taken for culturein liquid media MGIT Bactec 960 and solid media Lowenstein Jensen. The DNAs frompositive liquid media MGIT Bactec 960 were isolated and analyzed by spoligotyping toidentify the spoligo pattern. The spoligotyping results converted into octal format withinWords & Excel spreadsheets and compared to International Spoligotype-database(SpolDB4).The previous study (Parwati et.al.) found some differences geographical distributionbetween Beijing genotype strain of tubercle bacilli in West Indonesia compare to EastIndonesia, and the same pattern was also found in this study. Furthermore, the results inthis study showed the differences in spoligo pattern of Mtb complex at 10 urban areas inWest, Middle and East Indonesia. The percentage of Beijing strain family in the samplesfrom West Indonesia showed around 26.61% (31.48% in Sumatra, 28.83% in Java and16.98% in Kalimantan); from the Middle Indonesia around 25.93%; and none werefound in samples from the East Indonesia. The SpolDB4 pattern also showed that themajority of isolates belonged to major clades of M.tuberculosis, i.e. the East African-Indian (EAI); Haarlem (H), Latin American-Mediterranean (LAM), the Central andMiddle Eastern Asian (CAS); U = undefined; T = T family; and the MANU/ Manu family.We also found some isolates of Mycobacterium bovis. There were no significantassociation showed between genotype families and gender, but strong significantassociation found with ethnics and geography. Further confirmation of the results is stillBul. Penelit. Kesehat, Vol. 38, No. 4, 2010: 169 - 185170ongoing (κ value; RFLP and MIRU-VNTR). As conclusion, this study constituted a firstattempt to describe the preliminary mapping of genetic population structure of thetubercle bacilli circulating in Indonesia.Key words: preliminary mapping, Mtb complex, spoligotyping, Beijing genotype strain,SpolDB4