cover
Contact Name
Sri Hendrastuti Hidayat
Contact Email
-
Phone
+62 251-8621257
Journal Mail Official
jurnal.fitopatologi@apps.ipb.ac.id
Editorial Address
Jalan Kamper, Kampus IPB Darmaga,
Location
Kota bogor,
Jawa barat
INDONESIA
Jurnal Fitopatologi Indonesia
ISSN : 02157950     EISSN : 23392479     DOI : -
Core Subject : Health,
Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).
Arjuna Subject : -
Articles 318 Documents
Penyakit Kering Pelepah pada Tanaman Kelapa Sawit di Provinsi Kalimantan Timur dan Sumatera Utara Simanjuntak, Donnarina; Susanto, Agus
Jurnal Fitopatologi Indonesia Vol 9, No 3 (2013)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (410.927 KB)

Abstract

The rapid development of oil palm industry is indicated by wide monoculture plantation. This condition causes the development of many diseases such as basal stem rot, bud rot, leaf spot, anthracnose, and fruit rot diseases. Recently, a new disease causing dry frond symptom was reported from plantations in 2 provinces, i.e. East Kalimantan and North Sumatera. Disease exploration was conducted in Muara Wahau estate, East Kalimantan and Kota Pinang (Labuhan Batu) estate, North Sumatera. Typical symptoms involved decaying which started from the tip of frond that spread into the middle, rotting brown to blackish; when rot had reached the middle, the frond becomes dry, and finally broken. Isolation and identification of the fungi from the infected plant showed the association of Thielaviopsis sp., although Koch Postulate–based assay was unable to prove that the disease was caused by infection of Thielaviopsis sp.
Penggunaan Bakteriofag untuk Kit Detektor Patogen Hawar Bakteri Kedelai Dhany, Nurul Rama; Addy, Hardian Susilo; Wahyuni, Wiwiek Sri
Jurnal Fitopatologi Indonesia Vol 9, No 4 (2013)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (524.892 KB)

Abstract

Bacterial blight disease on soybean caused by Pseudomonas syringae pv. glycinea is an important factor causing yield loss in Indonesia. Bacteriophage isolated from irrigation water around the soybean field can be used as indicator for the presence of phytopathogenic bacteria. The objectives of this research was to obtain suitable composition of detector materials to detect P. syringae pv. glycinea using bacteriophage. Composition of detector kit contains of nutrient broth medium with 0.1% of bromothymol blue, 10 g talk and 1 g CMC which will be rubbed on to the detector paper and caused green colour development (pH ± 7) when the paper was dipped on to bacteria suspension, added by a drop of bacteriophage suspension and incubated for 24 hour, the colour will be changed. Yellow color indicated growth activity of P. syringae pv. glycinea where as blue colour indicated suppression of P. syringae pv. glycinea.
Khamir Antagonis untuk Pengendalian Penyakit Antraknosa pada Buah Avokad Selama Penyimpanan Fitriati, Yuli; Wiyono, Suryo; Sumarauw, Ivone Oley
Jurnal Fitopatologi Indonesia Vol 9, No 5 (2013)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (482.76 KB)

Abstract

Anthracnose caused by Colletotrichum gloeosporioides is an important disease in avocado fruit during storage. An effective, cheap, and safe control method is necessary as an alternative to subtitute the use of fungicides in postharvest disease control. This research aimed to identify yeast antagonist from avocados that are effective in controlling anthracnose disease on avocado fruit. Research was started with isolation of C. gloeosporioides and yeast from avocado fruit, followed by in vivo bioassay, antibiosis assay, and chitinolitic activity assay. In vivo bioassay was done by dipping avocado fruit on yeast cell suspension. As many as 23 yeasts isolates was obtained from avocado fruits. Eight yeast isolates (A28, A32, A33, A34, A35, A36, A37, A38) showed very effective for inhibiting anthracnose disease in avocado fruit at concentration of 106 mL-1 and 107 mL-1. However, only four isolates were chosen for further characterization based on morphological and molecular identification. Two species of yeast was identified as Pichia anomala, i.e. isolates A33 and A37 and Candida intermedia, i.e. isolates A35 and A36.
Sensitivitas Metode Serologi dan Polymerase Chain Reaction untuk Mendeteksi Bean Common Mosaic Potyvirus pada Kacang Panjang Anggraini, Sherli; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol 10, No 1 (2014)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (440.881 KB)

Abstract

Mosaic disease in yard long bean is caused by Bean common mosaic potyvirus (BCMV) and has been reported to affect yield. Common method to detect infection of BCMV involves serological assay and polymerase chain reaction (PCR). The aims of this research is to assess the sensitivity of three methods, i.e. Indirect Enzym-Linked Immunosorbent Assay (I-ELISA), Dot Immunobinding Assay (DIBA), and reverse transcription (RT)-PCR as detection method for BCMV infection in yard long bean. Sensitivity level of the methods was evaluated by diluting plant extract and antisera for I-ELISA and DIBA, and cDNA as template in RT-PCR. Virus isolate from Cirebon was maintained in yard long bean in screenhouse and used for the assessment. Absorbance value of ELISA showed that dilution end point for I-ELISA was reached at 10-3 and 10-2 of plant extract and antisera dilution, respectively. Positive infection was still detected using DIBA when the plant extract was diluted up to 10-5 based on development of color intensity on nitrocellulose membrane. Specific viral DNA fragment was still amplified when cDNA was diluted up to 10-4, indicated higher sensitivity level of RT-PCR method.
Cendawan Endofit Nonpatogen Asal Tanaman Cabai dan Potensinya sebagai Agens Pemacu Pertumbuhan Ramdan, Evan P.; Widodo, Widodo; Tondok, Efi Toding; Wiyono, Suryo; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol 9, No 5 (2013)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (339.983 KB)

Abstract

Endophytic fungi have been reported to have mutual symbiosis with their host. This research aimed to obtain nonpathogenic isolates of endophytic fungi from roots, stems, and branches of chili pepper. Endophytic fungi were isolated on 10% malt extract agar (MEA) after serial surface sterilization of plant parts. The effect of endophytic fungi on the development of chili pepper seeds were tested by growing seeds on media containing endophytic fungi isolate. As many as 138 isolates of endophytic fungi were successfully isolated from chili pepper plants from Garut and Bogor. Based on colony morphology the isolates can be differentiated into 9 morphotypes. Among the total isolates, 13.04% are nonpathogenic. Some of them (7,25%) do not effect seedling significantly compared to control and some isolates (5.79%) caused better seedling growth than control. Endophytic fungi having the potential to promote plant growth was identified as Aspergillus sp., Fusarium sp., Penicillium sp., sterile hyphae 1, 2, and 3.
Minyak Nilam sebagai Biofungisida untuk Pengendalian Penyakit Antraknosa Cabai Sakerebau, Desni Roha Miriam; Soekarno, Bonny Poernomo Wahyu Soekarno Poernomo Wahyu
Jurnal Fitopatologi Indonesia Vol 9, No 3 (2013)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (370.522 KB)

Abstract

Anthracnose caused by C. capsici is the most important factor causing low yield on chili in Indonesia. A research was conducted to examine to examine the potential of patchouli oil in suppressing anthracnose disease development on chili. In vitro test was conducted by growing C. capsici on potato dextrose agar (PDA) medium that has been added with different concentration of patchouli oil, i.e. 10, 5, 2.5, 1.25 and 0.625%. Negative controls treatment (Kn) was conducted by growing C. capsici on PDA without patchouli oil, whereas positive controls (Kp) was conducted by growing C. capsici on PDA containing mancozeb fungicide. Patchouli oil -A significantly suppressed the growth of C. capsici compared to patchouli oil-B on 10 days after harvest, i.e. 91.15% suppression for all concentration level except for 0.625% which caused 85.92% suppression. Further examination using patchouli oil-A showed that incubation period on curative test was 8 days after inoculation (DAI) whereas on preventive and induced resistance test were 5 DAI. Disease severity at 10 DAI reached 4.8%, 23.20%, and 29.60% on curative, preventive, and induced resistance tests, respectively. Disease severity was 29.60% and 68.80% on positive control and negative control, respectively.
Pengendalian Penyakit Budok dengan Fungisida dan Deteksi Residu pada Daun Nilam Sumardiyono, Christanti; Hartono, Sedyo; Nasrun, Nasrun; Sukamto, Sukamto
Jurnal Fitopatologi Indonesia Vol 9, No 3 (2013)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (397.973 KB)

Abstract

Budok caused by Synchytrium pogostemonis is the important and destructive disease on patchouli plant (Pogostemon cablin). Chemical control must be done in incidence with high disease intensity and prediction of outbreak. The experiment was done using benomyl (Benlate 50 WP) and cuprous oxide (Kocide 77WP) 0.1%, 0.2% and mixture of both ( 1 g cuprous oxide and 1 g L-1 benomyl). Patchouli plant was sprayed eight times with one week interval. Harvesting was done two weeks after the end of spraying. Residue analysis of Cu was done with AAS and benomyl residue with HPLC. The result showed that 0.1% benomyl significantly reduced the disease intensity. The other treatments showed lower result. Harvested patchouli plants treated with 0.1% benomyl showed higher yield compare with 0.2% benomyl and cuprous oxyde. Cu residue in dried leaves was between 460 ppm and 950 ppm. Residue of benomyl was 54–100 ppb. Diseased seedling still can be used after spraying by benomyl four times with 2 weeks interval.
Identifikasi Cendawan Endofit Menggunakan Teknik Polymerase Chain Reaction (Detection of Endophytic Fungi Using Polymerase Chain Reaction Technique) Legiastuti, Tuti Susanti; Aminingsih, Tri
Jurnal Fitopatologi Indonesia Vol 8, No 2 (2012)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1022.878 KB)

Abstract

Yellow leaf curl disease, caused by a member of Begomovirus (Geminiviridae), is one of important diseases of chilli pepper in Indonesia. Exploration of endophytic fungi was initiated in order to find biological control agents for an alternative control strategies of this disease. Isolates of endophytic fungi were collected from chilli pepper growing area in Sleman, Yogyakarta and further identification using molecular technique involving polymerase chain reaction (PCR) and DNA sequencing was performed. DNA fragments of ±500 bp were successfully amplified from 10 fungal isolates by PCR using primer pair ITS1/ITS4, but only 8 DNA sequences was obtained for further genetic analysis. Based on BLASTN analysis the endophytic fungi were identified as having the highest similarity with Pleosporaceae sp. (98%) for H1 isolate, Cercospora nicotianae (100%) for H5 isolate, ercospora piaropi (98%) for H11 isolate, Guignardia mangiferae (99%) for H16 isolate, Geomyces pannorum 95%) for H17 isolate, Diaporthe phaseoloru (99%) for H18 isolate, Dothideomycete sp. (100%) for K3 isolate, and Alternaria longissima (99%) for K10 isolate. Key words: Begomovirus, chillipepper, DNA sequencing, polymerase chain reaction
Penggunaan Bakteri Kitinolitik sebagai Agens Biokontrol Penyakit Busuk Batang oleh Rhizoctonia solani pada Tanaman Kedelai (Utilization of Chitinolitic Bacteria as Biocontrol Agent of Stem Rot Disease by Rhizoctonia solani on Soybean) Khaeruni, Andi; Rahman, Abdul
Jurnal Fitopatologi Indonesia Vol 8, No 2 (2012)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (403.706 KB)

Abstract

Biological control of stem rot disease caused by Rhizoctonia solani on soybean using chitinolitic bacteria is one of the environmentally friendly control methods of pathogen. The study was conducted to select chitinolitic bacteria and to test their ability to suppress stem rot disease and promote plant growth of soybean. A total of ten chitinolitic bacteria were tested in vitro, and five of isolates i.e: ST21e, SS12b, ST17c, ST27d, and ST26c found to have more than 30% suppression toward R. solani. In planta test under green house condition at six weeks after planting showed that soybean plants inoculated with ST27d and ST17c isolates had significantly lower disease incidence and had increased plant height and leaf number. Both of chitinolitic bacteria isolates are promising as biocontrol agents of stem rot disease caused by R. solani of soybean.Key words: biological control, chitinolitic bacteria, Rhizoctonia solani
Ketahanan Lapangan Padi Gogo terhadap Infeksi Curvularia oryzae Taufik, Muhammad; ., Asniah; ., Syair
Jurnal Fitopatologi Indonesia Vol 8, No 2 (2012)
Publisher : Jurnal Fitopatologi Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (308.615 KB)

Abstract

Southeast Sulawesi is known to have a wide varieties of local cultivars of upland rice. Unfortunately, resistance of those local cultivars of upland rice to Curvularia oryzae, one of the most important pathogen in upland rice cultivation, has not been reported. Resistance evaluation conducted in the field showed that local cultivars (Pae Kori, Pae Endokadia, and Pae Enggalaru) have high level of resistance, developing lines B10580E and B11580E have moderate resistance, whereas B11577E and TB368B lines were susceptible to C. oryzae infection.Key words: Curvularia oryzae, disease resistance, upland rice

Page 1 of 32 | Total Record : 318