Jurnal Ilmu Pertanian Indonesia
ISSN : 08534217     EISSN : 24433462
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Pencirian Mutu Kimiawi dan Mikrobiologis Produk Bandrek Instan dan Sirup Buah Pala (Myristica fragrans) Faridah, Didah Nur; Yasni, Sedarnawati; Suswantinah, Antin; Aryani, Ghesi Wuri
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The aim of this study was to find chemical and microbiological characteristics of instant bandrek and nutmeg syrup from Sinarsari and Dramaga villages, Dramaga District, Bogor Regency. The analysis resulted that both products still fulfilled the quality standard of microbiological set by the Indonesian National Standard. The antioxidant capacity of instant bandrek and nutmeg syrup consecutively was equivalent with 256.12 mg AEAC per serving size and 775.62 mg AEAC per serving size, respectively. The toal phenolic of the instant bandrek drink and nutmeg syrup consecutively was equivalent with 89.81 mg GAE/L and 140.68 mg GAE/L, respectively. It can be concluded that the food processing did not remove the bioactive compounds that could function as antioxidant. Keywords: antioxidant capacity, instant bandrek, nutmeg syrup, total phenolic level
Pencegahan Kerusakan Fisiologis Belimbing (Avverhoa carambola) dalam Rantai Pasok dengan Optimisasi Model Kombinasi Perlakuan Air Panas dan CaCl2 Menggunakan Response Surface Method Sutrisno, .; Darmawati, Emmy; Hasbullah, Rokhani; Prawaningrum, Harli
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Starfruits is one of exotic tropical fruits that have increasing its popularity both for domestic and global market due to its unique shape and taste, as well as their nutrition values. However, this fruit is considered perishable product with short self-life, thus the proper post harvest treatment should be done to prevent their quality after harvest. In general, the objective of this research was to determine optimum treatment of hot water treatment (HWT) and immersion period in CaCl2 solution using response surface method (RSM) for post harvest handling of starfruits in order to maintain its quality during storage. Among quality parameters tested, weight loss and hardness were found as the most responsive to the combination treatment. Parameter of weight loss rate showed a maximization response with stationary point of HWT’s temperature of 42 °C, expose period for 42 minutes, and immersion period in CaCl2 solution for 38 minutes with respond point of weight loss was 3.74%. Meanwhile, the hardness parameter achievement was the minimum with stationary point of HWT’s temperature of 48 °C, expose period for 35 minutes, and immersion period in CaCl2 solution fpr 35 minutes resulted respond point of hardness of 0.63 Newton. Interest level from the panelist were color, taste, texture and aroma, respectively, whereas the treatment combination that accepted by panelist were (45 °C, 65, 40), (45 °C, 40, 15), and (45 °C, 40, 40’). Keywords: CaCl2, hardness, HWT, RSM, starfruit, weight loss
Pemulihan Stok dan Restorasi Habitat Teripang: Status Ekosistem Lamun di Lokasi Restocking Pulau Pramuka dan Pulau Kelapa Dua, Kepulauan Seribu, Jakarta Taurusman, Am Azbas; Isdahartati, .; Isheliadesti, .; Ristiani, .
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Global fish stock has been significantly declined over the past 3 decades, especially in coastal waters. Over-exploitation of fish resources and habitat destruction has been considered playing important role in declining the fish stock. This study was conducted to restore sea cucumbers stock in Pulau Seribu. A base line study was needed to evaluate the status of seagrass ecosystems in restocking locations. The results showed there were 8 seagrass species identified in both study sites, covering 0-65% area, consisted of 20.45 ± 11.28% in Pulau Pramuka and 6.00 ± 6.56% in Pulau Kelapa Dua, respectively. Seagrass ecosystem in Pulau Pramuka indicated better condition than that in Pulau Kelapa Dua and statistically was significantly different (global R = 0.193; p < 0.001). However, to support the restocking efforts of sea cucumbers, the seagrass habitat in both locations need to be restored. Keywords: restoration habitat, sea cucumbers restocking, seagrass ecosystem status
Pengaruh Laju Pendinginan, Suhu, dan Lama Kristalisasi pada Profil Triasilgliserol dan Sifat Pelelehan Produk Fraksionasi Minyak Kelapa Mursalin, .; Hariyadi, Purwiyatno; Purnomo, Eko Hari; Andarwulan, Nuri; Fardiaz, Dedi
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Dry fractionation will produce a fraction of olein and stearin with different composition of high melting and low melting as well the distribution of triacylglycerol (TAG) in the form of trisaturated (St3), disaturated (St2U) and monosaturated (StU2). This research aimed to study the effect of critical cooling rate and crystallization temperature on the composition and profile of TAG changes as well the melting properties of the fractionated coconut oil products. Coconut oilwas heated at 70°C then cooledat differentcooling rate to reach various crystalization temperatures. The oil was then stirred at 15rpm and allowed to crystallize at different period of time, and finally fractionated by vacuum filtration using Whatman #40 paper. Fractionation temperatures were the same as crystalization temperatures. The results showed that the critical cooling rate was proportional to the solid-like to liquid-like ratio, the content of St3 and SFC profile of olein fraction but inversely proportional to the content of St2U and StU2 TAG. Interval crystallization temperature between 21.30 and 21.73 °C produced the S/L ratio, the content of St3 TAG and SFC profiles of olein fractions lower and the content of St2U and StU2 TAG higher than the temperature interval below or above it. Coconut oil fractionation more effective in higher crystallization temperature or lower critical cooling rate. In these cooling treatments, St3 TAG which has high melting pointwould be concentrated at stearin fraction, while St2U and StU2 TAG and MCTwould be at olein fraction. Therefore, it will increase melting properties of stearin fraction and decrease olein fraction. Keywords: coconut oil, critical cooling rates, crystallization temperature, fractionation, melting properties, TAG profile
Ekspresi Enzim Rekombinan Reverse Transcriptase (RTRNase H) Simian Betaretrovirus Serotipe-2 Asal Macaca fascicularis Indonesia dalam Sistem Ekspresi Eschericia coli Saepuloh, Uus; Iskandriati, Diah; Pamungkas, Joko; Sajuthi, Dondin
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Reverse transcriptase (RT) enzyme is a key tool in molecular biology for the synthesis of complementary DNA (cDNA) from messenger RNA (mRNA). Combining RT activity with PCR amplification has been a gold standard as the first step in cloning the coding region of any gene of interest. Evidently, RTs have been critical in advancing molecular biology, genetics and medicine to their current stage. In this study, we were developing the RTDRNase H recombinant enzyme isolated from serotype-2 simian betaretrovirus-2 (SRV-2) infected Indonesian Macaca fascicularis using Escherichia coli expression system. The study was conducted using RT SRV-2 gene expression using E. coli expression system, proteins purification, and application to RT PCR technique. The SDS PAGE expression analysis showed a specific band size of 32.7 kDa assumed as RT protein enzyme. Application of RT SRV-2 enzyme generated to the RT PCR technique of β-globin CDV and SRV-2 env gene target showed that the RT SRV-2 enzyme was capable to reverse transcribed mRNA into cDNA as indicated by the presence of specific DNA band compared with commercial RT enzymes. This RT SRV-2 enzyme showed its activity similar to that of commercial one, although the activity was lower. Keywords: escherichia coli expression system, recombinant enzyme, reverse transcriptase, SRV-2
Ciri Limbah Pemanenan Kayu di Hutan Rawa Gambut Tropika Suwarna, Ujang; Matangaran, Juang Rata; Morizon, .
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Effectiveness of timber harvesting in peat swamp forest is low. It is indicated by highly logging waste caused by timber harvesting operation. Thus, logging waste should be studied to describe characteristic and volume of logging waste in locations of compartment, landing, skidding track, and hauling road. The waste can be in the form of stumps, stems, and branches with diameter above 30 cm. Data of the waste was collected from 6 sample plots consisted of 3 plots in mechanized plots and 3 plots in traditional plots. Most of the waste found in location of compartment that caused by felling activities. Average volume of waste in traditional plots was 7.81 m3/ha that was found in location of compartment. The average volume of waste in the mechanized plots was 19.75 m3/ha, consisted of 16.90 m3/ha (85.57%) in location of compartment and 2.85 m3/ha (14.43%) in landing point. Logging waste can be minimized by providing appropriate training regularly and increase techniques of timber harvesting in the field. Keywords: logging waste, peat swamp forest, timber harvesting
Pemanfaatan Ekstrak Daun Erpa (Aerva sanguinolenta) untuk Label Cerdas Indikator Warna Warsiki, Endang; Nofrida, Rini; Yuliasih, Indah
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The color of natural dyes, as anthocyanins, is very easily changing as he temperature changes. The color change can be used as a color indicator to provide information about product deterioration due to its sensitivity toward temperature and light. This indicator is called smart label of color indicator. This film label can be made of chitosan and polyvinyl alcohol and extract of erpa (Aerva sanguinolenta) leaves. The extraction was done under various leaf:water ratios. The filming was done in 2 methods, i.e. (i) by mixing the extract into film solution and drying the film at 50 °C; and (ii) by brushing the extract on the film surface. The leaf extract had a pH of 5.09 and the total yield of 116.65 mg anthocyanin/100 g leaves. Filming of color indicator in mixing method and dried at 50 °C resulted a colorless film. Brushing method was the best method to produce smart label with quite stable property and 1 mL extract could be applied evenly per 40 cm2 film area. Thus, this film can be utilized as smart label with color indicator to detect the deterioration in temperature-sensitive products. Keywords: color indicator, erpa leaf extract, smart label
Analisis Layer System Bambu Laminasi Berdasarkan Penyebaran Kerapatan Ikatan Pembuluhnya Bahtiar, Effendi Tri; Nugroho, Naresworo; Surjokusumo, Surjono; Karlinasari, Lina; Darwis, Atmawi
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Anatomically, bamboo is constructed from many types of cells. Vascular bundles are the cells which give the highest contribution to the bamboo strength, thus the density distribution of vascular bundles could be used as the main variable for analyzing the layer system of bamboo strip and laminated bamboo. The density of vascular bundles distribution decreases gradually from the outer to inner part in a regular manner which could be fitted by linear or nonlinear function. Ratio of modulus of elasticity (E) which widely used in transformed cross section (TCC) method for analyzing the layer system are substituted by ratio of density distribution of vascular bundles with asumption that both are highly correlated. The data in this study proved that there are high Pearson’s corellation between the theoretical and the empirical result, and the paired t-student test also showed both are not significantly different; thus, this new method could be applied in very good result. There are 3 models applied in this study, namely linear, logarithm, and power. Power model is the best among others since its theoretical gives the nearest estimation to the empirical measurement. Keywords: bamboo and laminated bamboo, mechanical properties, model, transformed cross section, vascular bundles
Pemeliharaan Ikan Sidat dengan Sistem Air Bersirkulasi Affandi, Ridwan; Budiardi, Tatag; Wahju, Ronny Irawan; Taurusman, Am Azbas
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Today, eel resource especially seeds in Indonesia has not been used for eel culture activities. To be able to optimally utilize the seeds that led to the production of eels for consumption needs adequate cultivation technology. This study aimed to obtain performance information of survival rate, growth rate, and feed conversion ratio to support mass production of eel consumption. The experiments were performed using aquarium of (0.8 x 0.4 x 0.4) m3 and concrete tank (1.7 x 1.7 x 1) m3 with circulating water. Eel used were elver (1.2-1.5 g) and fingerlings (15-17 g). Silk worms (Tubifex) and artificial feed in the pasta form were used as feed. The results showed that the elver reared in aquarium or concrete tank with water recirculation system showed high survival rate of 78-79% and 86-96%, respectively. The specific growth rate (SGR) was good (0.6-0.8%), but the feed conversion was still high (33-21) for the elver fed with silk worms and very good (0.6-0.7) for elver fed with artificial feed. The juvenile eel reared in a concrete tank showed SR up to 85-94%, the SGR ranged from 0.8 to 1.2%, and feed conversion from 0.61 to 0.69. It can be concluded that the rearing of seed eel can be done incontainer using water recirculation system with stocking density of 3 individuals/land 1.5 kg/m3 in preparing the seed to be ready tobe cultivated outdoors. Keywords: elver, feed conversion ratio, juvenile, specific growth rate, water recirculation system
Pemulihan Stok dan Restorasi Habitat Teripang: Status Ekosistem Lamun di Lokasi Restocking Pulau Pramuka dan Pulau Kelapa Dua, Kepulauan Seribu, Jakarta Taurusman, Am Azbas; Isdahartati, .; Isheliadesti, .; Ristiani, .
Jurnal Ilmu Pertanian Indonesia Vol 18, No 1 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Original Source | Check in Google Scholar | Full PDF (322.848 KB)

Abstract

Global fish stock has been significantly declined over the past 3 decades, especially in coastal waters. Over-exploitation of fish resources and habitat destruction has been considered playing important role in declining the fish stock. This study was conducted to restore sea cucumbers stock in Pulau Seribu. A base line study was needed to evaluate the status of seagrass ecosystems in restocking locations. The results showed there were 8 seagrass species identified in both study sites, covering 0-65% area, consisted of 20.45 ± 11.28% in Pulau Pramuka and 6.00 ± 6.56% in Pulau Kelapa Dua, respectively. Seagrass ecosystem in Pulau Pramuka indicated better condition than that in Pulau Kelapa Dua and statistically was significantly different (global R = 0.193; p < 0.001). However, to support the restocking efforts of sea cucumbers, the seagrass habitat in both locations need to be restored.

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