Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
ISSN : 20852916     EISSN : 23373652
urnal Agronomi Indonesia, sebelumnya bernama Buletin Agronomi, terbit sejak 1962, diterbitkan oleh Departemen Agronomi dan Hortikultura Fakultas Pertanian, Institut Pertanian Bogor dan Perhimpunan Agronomi Indonesia (PERAGI). Jurnal Agronomi Indonesia merupakan media untuk publikasi tulisan asli yang berkaitan dengan budidaya tanaman secara luas, dalam Bahasa Indonesia maupun Bahasa Inggris. Naskah dapat berupa: hasil-hasil penelitian mutakhir (paling lama 5 tahun yang lalu), ulasan (review)singkat, analisis kebijakan atau catatan penelitian (research note) singkat mengenai teknik percobaan, alat, pengamatan, hasil awal percobaan (preliminary result).
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Search results for , issue " Vol 38, No 1 (2010): Jurnal Agronomi Indonesia" : 23 Documents clear
Seleksi Genotipe Padi Mutan Insersi Toleran Cekaman Salinitas Berdasarkan Karakter Pertumbuhan dan Biokimia Situmorang, Apriadi; Zannati, Anky; Widyajayantie, Dwi; Nugroho, Satya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Eleven genotypes of insertional mutant Nipponbare rice carrying randomly inserted activation tag were tested to investigate the growth and biochemical responses to salt stress. Pokkali, IR 29, and non-transgenic Nipponbare were used as tolerant, susceptible, and isogenic control, respectively. Plants were grown in plastic trays filled with 0  g L-1 NaCl  Yoshida nutrient solution as control and grown in 6 g L-1 NaCl as treatment. Salt injury was scored on 10, 16, and 21 days after salinization. Observation at 21 days after salinization showed that there was significant  difference  among genotypes in growth and biochemical characters. Classification  using hierarchical cluster analysis based on growth and biochemical responses showed that there were three clusters. Cluster I consisted of wild-type Nipponbare, T2.pMO.V.3.13.c.22, T2.pMO. V.1.7.a.15, T2.pMO.III.98.b.7, T2.pMO.II.231.c15, T2.pMO.III.98.b.8, T2.pMO.III.98.b.17, T2.pMO.III.98.b.3, and T2.pMO.VI.81.3.a.4. Cluster II consisted of IR 29, T2.pMO.V.3.13.c.14, T2.pMO.VI.30.1a.125, and T2.pMO.VI.30.1.a.65. However no mutant genotype was clustered with Pokkali in cluster III, which may indicate that there was no salt tolerant mutant genotype found in this research.   Keywords: salt tolerance mutant rice, growth characters, biochemical characters
Induksi dan Proliferasi Kalus Embriogenik pada Beberapa Genotipe Kedelai Khumaida, Nurul; Handayani, Tri
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The successful of gene transformation on crop plants depends on the ability of explant to develop somatic embryos. The effect of medium composition on induction and proliferation of embryonic callus was analyzed on several soybean genotypes, including low irradiance (LI) tolerant genotype (Ceneng, Pangrango C6-30-10 and C6-76-10) and two LI sensitive genotypes (Godeg and Slamet). Immature cotyledons (14 DAA) were cultured in induction medium including MSIA (MS, vitamin B5, 30% sucrose, 0.2% gelrite, 10 mg L-1 2,4-D and 10 mg L-1 NAA) and MSIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 40 mg L-1 2,4-D). Embryonic calli was subcultered one month after initiation onto proliferation medium including MSIIA (MS, vit. B5, 30% sucrose, 0.2% gelrite, 5 mg L-1 2,4-D and 5 mg L-1 NAA) and MSIIB (MS, vitamin B5, 30% sucrose, 0.2% gelrite, and 20 mg L-1 2,4-D). The result showed that percentage of callused explant was 76-94% and the highest initiation obtained on Pangrango genotypes. The average of calli diameter at 1 month after initiation was 0.5-1.2 cm. Calli which was obtained on MSIA medium showed yellow, tranparent, and friable, whereas calli was obtained on MSIB medium showed yellow-brown, transparent, and friable. Increasing calli diameter and structure were obtained on proliferation medium. The combination of 2,4-D and NAA on MSIA and MSIIA both are induction and proliferation medium respectively were better than MSIB and MSIIB which were contain only 2,4-D. Ceneng genotype showed best performance of somatic embryogenesis than others, and Slamet genotype showed lowest response both on callus induction and proliferation.   Keywords: immature cotyledons, embryonic callus, NAA, 2,4
Efektivitas Ekstrak Tumbuhan untuk Mengeliminasi Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Zainal, Aprizal; Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, ,; Giyanto, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Objectives of experiments were to evaluate (1) in vitro inhibitory effects of plant extracts on Clavibacter michiganensis subsp. michiganensis (Cmm), (2) inhibitory effects of plant extracts on Cmm infected tomato seeds, and (3) effectiveness of seed treatment plus plant extracts to eliminate Cmm. After evaluating 20 plant extracts, curcuma rhizome and betel vine leaf extract, cinnamon and clove oil were selected for further test. Tomato seeds were artificially inoculated with Cmm to obtain high level of infection. Part of the seeds were dipped in either suspension of selected extracts for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 °C and RH 60-70%) plus either of plant extracts oils, respectively. Elimination of Cmm level from infected seeds was observed at 10 days after treatments. Results of the experiment indicated curcuma extract, betel vine extract, cinnamon oil or clove oil showed in vitro inhibitory effects on Cmm. Moreover, dipping infected seeds in either 5% of curcuma, betel vine extract, or 0.5% of clove oil or matriconditioning plus these extracts oils were effective to eliminate Cmm from infected seeds. These treatments may potentially be used and developed commercially for eliminating seedborne Cmm on infected tomato seeds.   Keywords: Bacterial cancer, seedborne-pathogen, seed-treatments
Karakter Fisik dan Kimia Buah Pepaya pada Stadia Kematangan Berbeda Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The objective of the experiment was to investigate the physical and chemical characteristics of three stadia of maturity based on a range of peel color from green to yellow or based on percentage of the yellow area of fruit peel (stadium 1 = 25-49 % yellow, stadium 2 = 50-74 % yellow, and stadium 3 = above 75 % yellow) on six genotypes of papaya. Each genotype exhibited different days to maturity for each stadium. The fruits of stadium 1, 2 and 3 for IPB 1 were picked at 130, 135, and 140 days after anthesis (DAA); IPB 10A at 160, 165, and 170 DAA;  IPB 1 x PB 174 at 135, 140 and 145 DAA; while PB 174,  IPB 1 x IPB 10A and IPB 10A x PB 174 were picked at 140, 145 and 150 DAA, respectively. The results indicated that peel firmness was affected by maturity stage on female fruit of IPB 10A. Maturity stage affected chemical characteristics of papaya included total soluble solids (TSS) content (IPB 10A, female fruit of  PB 174, female fruit of IPB 1 x IPB 10A, and female fruit of IPB 1 x PB 174), vitamin C content (hermaphrodite fruit of 10 A, female fruit of IPB 1 x IPB 10A) and juice pH (hermaphrodite fruit of  IPB 1).  IPB 1 genotype can be harvested at all stadia of maturity stage. Hermaphrodite and female fruit of IPB 10 A, female fruit of  PB 174, female fruit of  IPB 1 x IPB 10A and female fruit of IPB 1 x  PB 174 genotype would be better harvested at stadium 3 of maturity stage.   Keywords: Carica papaya, papaya genotype, hermaphrodite fruit, female fruit, fruit quality, fruit maturity stage
Pengaruh Bioaktivator terhadap Pertumbuhan Sukun (Artocarpus communis Forst) dan Perubahan Sifat Kimia Tanah Gambut Utomo, Budi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

This research was aimed to detect effectiveness of microorganism to increase growth of Artocarpus communis in peat soil. The peat soil came from countryside of Sei Toras, District Panai Tengah, and District Labuhanbatu, North Sumatra Province. Activators tested were Aspergillus sp., EM4, MOD-71, Supernasa and Puja-168. The research was done at Faculty of Agriculture, the University of North Sumatera from February to April 2009. Application of Aspergillus sp. Increased plant height (31.24%), stem diameter (2.84%) and leaf area (63%). Compared to control treatment, the application of Aspergillus sp. increased available P 49%, K 34%, and Ca 45%, however level of total N decreased by 17%.   Keywords: Aspergillus sp., decomposer, nutrient availability, Effective Microorganism, bioactivator
Evaluasi Daya Hasil Cabai Hibrida dan Daya Adaptasinya di Empat Lokasi dalam Dua Tahun Syukur, Muhamad; Sujiprihati, Sriani; Yunianti, Rahmi; Kusumah, Darmawan Asta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The aim of the experiment was to study the performance of yield components of  eight pepper hybrids and their adaptation at four locations in two years (2008 and 2009). The experimental design used was Randomized Complete Block Design (RCBD), three replications as a block was nested in the location. Plant materials were eight hybrids (IPB CH1, IPB CH2, IPB CH3, IPB CH25, and four commercial hybrids were Adipati, Biola, Gada, and Hot Beauty). The IPB CH3 hybrid produced higher fruit width, fruit length, fruit weight, fruit flesh density and yield per plant compared to commercial hybrids. Days to flowering and days to harvesting of IPB CH3 was earlier than that of Hot Beauty. The performance of yield characters in Boyolali, Rembang and Subang was better than that in Bogor. The IPB CH3 hybrid was specifically adapted to Subang and Rembang, while IPB CH1, IPB CH2, IPB CH25 were suitable for Boyolali.   Keywords: fruit, yield components, genotype x location interaction, Capsicum annuum L.
Interaksi Genetik x Lingkungan dan Stabilitas Komponen Hasil Berbagai Genotipe Kedelai di Provinsi Riau Rasyad, Aslim; Idwar, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Genotype by environment (GE) interaction and stability of a trait in any crop plant such as soybean (Glycine max L. Merr.) are very important for plant breeders to develop and evaluate the new cultivars as well as for farmers to plant suitable cultivars for commercial purpose. Crop performances including harvesting date, yield components and grain yield of nine genotypes of soybean were evaluated at three locations with distinct environments in Riau. The data were used to determine GE interaction variance components of the traits and yield stability.  There was significant effect of location on all characters except on grain yield per plot.  The genotypes differed significantly in all yield components and grain yield.  Genotype x environment interaction significantly affected several crop performances such as harvesting date, all yield components and grain yield.  The magnitude of GE interaction variance component was greater than that of location for all traits except the number of seed per plant indicating that most genotypes performed differently across the locations and were not stable with respect to the locations.  Among the nine genotypes, line 19BE and Malabar are classified as stable genotypes and could be grown in wide area of Riau Province, while line 13ED and Kipas Putih produced high grain yield in specific area and could be only grown in Pekanbaru.   Keywords: genotype x environment interaction, yield components, stability, soybean 
Respon Rumput Benggala (Panicum maximum L.) terhadap Gypsum dan Pupuk Kandang di Tanah Salin Purbajanti, Endang Dwi; Soetrisno, Djoko; Hanudin, Eko; Budhi, Subur Priyono Sasmito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The research was aim at studying the effect of application of gypsum and manure to growth, biomass yield, dry matter yield, dry matter content and crude protein of Benggala grass. This study used factorial design with nine replications. The first factor was manure dosages i.e. 0 and 20 ton ha-1. The second factor was gypsum dosages i.e. 0, 0.75, 1.5 and 3 ton ha-1. Variables to observe were relative growth rate (RGR), biomass yield, dry matter yield, dry matter content and crude protein. The application of 20 ton manure and 3 ton gypsum per hectare resulted the highest values of  RGR, biomass yield, dry matter yield, dry matter content and crude protein; they were 0.86 g day-1, 1039.4 g pot-1, 219.1 g pot-1, 20.9% and 8.1%, respectively. Response of RGR, biomass yield, dry matter yield, dry matter content and crude protein to gypsum application with 20 ton manure  ha-1 were linier.  Keywords :  benggala grass,  saline soil, gypsum, manure, nitrogen.
Isolasi dan Pengklonan Fragmen cDNA Gen Penyandi H+-ATPase Membran Plasma dari Melastoma malabathricum L. Muzuni, ,; Sopandie, Didy; Suharsono, Utut Widyastuti; Suharsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Melastoma malabathricum L. grows well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying acid and aluminum stress is a plasma membrane H+  -ATPase protein encoded by PMA gene. The objective of this research was to isolate and clone the cDNA fragment of MmPMA encoding plasma membrane H+ -ATPase from M. malabathricum L. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of MmPMA  cDNA  had been successfully isolated by PCR by using total cDNA  as  template and PMA primer designed from conserved region for corresponding gene. This fragment had been successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5". Nucleotide sequence analysis showed that the length of MmPMA fragment is 806 bp encoding 268 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MmPMA fragment was 81% identical to part of PMA of Sesbania rostrata, Juglans regia, and Prunus persica. Based on deduced amino acid sequence, MmPMA was 94% identical to part of PMA of Juglans regia; 93% to PMA of S. rostrata, and Arabidopsis thaliana. MmPMA fragment has phosphorylation intermediate domain (DKTGT) and ATP binding domain (KGAP, DPPR, MITGD, and GDGVN).   Keywords: isolation, Melastoma malabathricum L., MmPMA fragment, sequencing
Transformasi Padi Indica Kultivar Batutegi dan Kasalath dengan Gen Regulator HD-Zip untuk Perakitan Varietas Toleran Kekeringan Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; Ouwerkerk, Pieter B.F.; Loedin, Inez Hortense Slamet
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 38, No 1 (2010): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

water deficiency. Genetic engineering at the level of transcription factors (TF) is particulary a promising strategy in developing drought tolerant rice cultivar. HD-Zip genes are TF that function in plant adaptation to some environmental stresses including water deficit. The recombinant plasmid pC1301H Oshox6 which contained HD-Zip Oshox6 gene was placed under a drought inducible promoter called LEA promoter, gusA and hpt genes were driven with CaMV promoter. The aim of research was to obtain indica rice transgenic plants of Batutegi and Kasalath cultivars using pC1301H Oshox6 plasmid. Recombinant plasmid was transformed into immature rice embryos using Agrobacterium tumefaciens. Kasalath cultivar showed a better capacity to form embryogenic calli compared to Batutegi. Transformation efficiency of Batutegi is lower (1.5 - 0.3%) than Kasalath (2.2-28.3%). Regeneration efficiency is 25-83.3% and 7.7-100% for Batutegi and Kasalath, respectively. Number of putative transformant plantlets of Batutegi and Kasalath are 63 and 48 plantlets, respectively. Southern blot analysis (using hpt probe) on 12 independent lines of each Batutegi and Kasalath cultivars showed different gene copy number, ranging from one to four copies of gene.   Keywords: agrobacterium tumefaciens, LEA promoter, HD-Zip Oshox6, rice

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