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Jurnal ILMU DASAR
Published by Universitas Jember
Jurnal ILMU DASAR (JID) adalah jurnal ilmiah yang melingkupi bidang matematika, fisika, kimia dan biologi. Naskah yang diusulkan untuk diterbitkan Jurnal Ilmu Dasar adalah naskah yang belum pernah diterbitkan dan atau tidak sedang dipertimbangkan penerbitannya di majalah lain. Jurnal Ilmu Dasar menerima tulisan hasil penelitian dasar (bukan aplikasi) untuk bidang matematika, kimia, fisika, dan biologi. Naskah ilmiah ditulis dalam bahasa Indonesia atau dalam bahasa Inggris yang baik. Secara lebih detail dapat dilihat dari pedoman penulisan.
Articles
415
Articles
Regeneration Rate of Eggplant Somatic Embryogenic In Various Maturation Media

Hartati, H., Hartati, N. Sri, Sudarmonowati, Enny

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Ralstonia solanacearum is one of the most important pathogen that causes bacterial wilt disease in eggplant and inhibits eggplant production. Improvement of eggplant varieties resistant to bacterial wilt can be accomplished through genetic manipulation. Regeneration of in vitro plants isone of the important tools to supports plant improvementthrough biotechnology. This study was aimed to determine the rate of eggplant regeneration in various maturation media, and to find the best medium for eggplant regeneration based on maturation rate and the number of cotyledon produced. We used resistant eggplant (accession 032) as the material to produce somatic embryogenic.There were 7 types of regeneration media used in this research. MS medium was supplemented with a certain concentration of plant growt regulators , such as: 1 mg / L + BAP 1 mg / L, NAA 4mg / L, TDZ 0.005 mg / L, TDZ 0.001 mg / L, CuSO4 2mM + BAP 1 mg / L, CuSO4 2mM + BAP 2 mg / L and Kinetin 1 mg / L + CuSO4 2mM. Three clumps of callus per plate with three replications were transferred to MS suplemented medium. The parameters observed were the color of callus before and after they were transfered to regeneration medium, the day of formation of globular, heart-shaped, tubular and cotyledonary phase, and the number of cotyledons formed. The results obtained showed the somatic embryogenic color of the 032 genotype was white with friable structure before being transferred to regeneration medium and was turned to yellowish white after being transferred to the regeneration medium. On the day sixth, friable embryogenic somatic of eggplant was developed into nodule on medium MS + NAA 4 mg / L, MS + CuSO4 2mM + BAP medium 1 mg / L, and MS + CuSO4 2mM + BAP 2 mg / L. Somatic embryogenic callus of accession 032 were able to pass complete globular, heart-shaped, tubular and cotyledonary phase. The most responsive medium for somatic embryogenic callus regeneration, based on the days of the callus phases formation and the number of early-phase cotyledons obtained, were MS medium suplemented with CuSO4 2mM + BAP,and CuSO4 2 mM + BAP 2 mg / L.Keywords: eggplant, Ralstonia solanacearum, regeneration, cotyledonary, clump, BAP

Optimization Of The Annealing Temperature With Degenerate Primer For Amplification Of Arginine Decarboxylase (ADC) Fragment Gene From Genomic DNA of Maluku Tenggara Local Cassava

Kurniawati, Siti, Hartati, Sri

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Arginine decarboxylase (ADC) is a key enzyme responsible for polyamines biosynthesis and has been shown to increase resistance to biotic and abiotic stress. Cassava (Manihot esculenta Crantz.) is able to grow and produce storage roots well on marginal land. The purpose of this study was to optimize annealing temperature of primers in PCR reaction to amplify candidate cassava ADC gene fragments. Annealing temperature is a crucial factor in PCR reaction affecting product (gene fragments) specificity. Four pairs of primers; MeADC1, MeADC2, MeADC3, andMeADC4, were designed using degenerate method from several plants species such as Jatropa curcas (Acc XM_022220421), Populus trichocarpa (Acc XM_002306105.2), Capsicum annuum cv Nockwang (Acc KC160547.1) and Lycopersicon esculentum (Acc L16582.1). All primer pairs successfully amplified DNA fragments from local cassava genotypes (Maluku Tenggara/Malra) including Malra012 and Malra016. The MeADC1 primer amplified DNA fragment with less than 1,000 base pairs (bp) at annealing temperature of 46°C, 47°C and 48°C. However, analysis of PCR product sequencing results using NCBI BLAST method showed that the amplified DNA fragment encodes for ribosomal protein S3 of Oryza minuta (Acc YP_009242005.1).Keywords: arginine decarboxylase, annealing, ADC, cassava, Maluku Tenggara, PCR

Investigations on The Mechanism of Artificial Photosynthesis of Ca-Pc-PDI and Dendrimer Molecule by DFT Calculations

Gunawan, Rahmat, Hammamiyah, Ulinnuha, Fadillah, Fahmi, Saleh, Chairul, Sitorus, Saibun

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Artificial photosynthesis modelling of Ca-Pc-PDI complex Ca Phthalocyanine Perylenediimide), and dendrimer molecule using Density of Functional Theory (DFT) Method has been studied to showed the energy efficiency of these compounds in terms of electron transfer in photosynthesis. The Analysis of Ca-Pc-PDI and dendrimer compound and also chlorophyl has been done in all computations using the GAMESS-US software. The computations result in this research showed that the large wavelength complex compounds of Ca-Pc-PDI obtained was 138.3299 nm and energy efficiency obtained was 0.89 eV. The data analysis states that the absorption of harvest light energy of complex compounds Ca-Pc-PDI lies in the far UV spectrum. The other side, the polyphenylene dendrimer structure molecular orbital analyses it was found that the dendrimer was capable of electron transfers as indicated by the existence of HOMO and LUMO and result comparisons with chlorophyll. UV wavelengths of the polyethylene dendrimer and chlorophyll, respectively, suggesting that the polyphenylene dendrimer is capable of substituting chlorophyll in artificial photosyntheses. We can states from the result that these compound ability to be applied in the modeling of artificial photosynthesis as a material of energy absorption that mimics the workings of chlorophyll in terms of electron transfer in natural photosynthesis process. Keywords: Artificial photosynthesis modelling, Ca-Phthalocyanine-Perylenediimide complex, and dendrimer molecule

FTIR and Moisture Absorption of Yam Bean Starch Biocomposites with Yam Bean (Pachyrhizus erosus) Bagasse Fibers as Reinforcement

Mahardika, Melbi, Abral, Hairul, Kasim, Anwar, Arief, Syukri, Asrofi, Mochamad

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Biocomposites from yam bean starch with yam bean bagasse (YBB) fibers as reinforcement has been successfully fabricated. The fabrication method was solution casting. YBB was variated for 1, 2, 3 and 4wt% (from dry weight starch basis). 2 mL glycerol was used as a plasticizer. The relative humidity (RH) condition in moisture testing was 99%. The result shows that the addition YBB able to decreased moisture absorption of starch film. The lowest moisture absorption was in biocomposites with 4wt% YBB. The moisture absorption test was supported byfourier transform infrared (FTIR) data.Keywords: Biocomposite, yam bean, yam bean bagasse, moisture, FTIR

Phase Diagram and Thermodynamic Properties of Ketoprofen-Succinic Acid Binary Mixtures

Wicaksono, Yudi, Setyawan, Dwi, Siswandono, S.

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The equilibrium phase diagram and thermodynamic properties of a mixture of drugs and additives are information related to various possible interaction processes between components. Therefore, we conducted a study of the phase diagrams and thermodynamic properties of binary mixtures of ketoprofen-succinic acid to estimate the types of interactions that may occur between these materials. The solid-liquid phase diagram of ketoprofen-succinic acid binary mixtures was determined by differential scanning calorimetry and composition of eutectic system was determined accurately using a Tamman diagram. The measurement of binary mixtures of ketoprofen-succinic acid with differential scanning calorimeter obtained the value of melting temperature and heat of fusion of ketoprofen- succinic acid system. The solid-liquid phase diagram of ketoprofen- succinic acid showed the formation of eutectic type phase diagram. The Tamman diagram showed accurately composition of the eutectic system of the Kp-SA binary mixtures at the mole fraction of Kp 0.87 and temperature 96.9oC.Keywords: ketoprofen, phase diagram, eutectic system, Tamman diagram

Synthesis of Zeolite A From Coal Fly Ash with Variation of Si/Al Molar Ratio

Andarini, Novita, Haryati, Tanti, Lutfia, Zuhrotul

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Fly ash containing 30-36% silica and 14,52-23,78% alumina can be potentially as raw material for synthetic zeolite such as zeolite A. Zeolite A is an aluminosilicate mineral which is rich in alumina so that this zeolite has a good cation exchange capability. Zeolite A has been synthesized by hydrothermal treatment after NaOH fusion. Fly ash has been fused with NaOH at 550 0C for 40 minutes and hydrothermally treated at 1000C for 5 hours. The hydrothermal treatment was conducted in some various Si/Al molar ratios from 0.90; 1.00.; 1.05; to 1.24. The zeolite A was then analyzed using XRD and XRF. The best zeolite A based on XRD result is zeolite with Si/Al molar ratio of 1.1 with crystallinity of 96,80%. The x-ray fluorescence result showed that the Si/Al molar ratios of the four zeolite samples were close to of Si/Al molar ratios of 1, 1.1, 1.21.3 respectively.Keywords: Fly ash, Zeolite A, Hydrothermal Fusion

Design of Sound Level Meter Using Sound Sensor Based on Arduino Uno

Lapono, Laura Anastasi Seseragi, Pingak, Redi Kristian

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Sound Level Meter (SLM) is a tool used to measure the noise level for a moment. For improved performance, hence required a measure of noise level capable of displaying result automatically on the computer so that simplify user to observe and measure the noise. In this study, the design system of data acquisition consists of a MAX4466 sound sensor, Arduino UNO microcontroller, and computer to display the measurement result. The measurement results are displayed in the form of data and graphs. The display of software designed using the Delphi 7.0. The process of taking data in the room with a sound intensity of 44.6 dB. The value is the measurement result using the SLM tool, while the measurement results using the sound sensor performed every second during an interval of 30 seconds obtained an average of 44.19 dB. It can be seen that between the two results shows a relatively small difference, so it can be concluded that the design of this system is running well. Keywords: Noise, SLM, Sound sensor, Arduino Uno

Using Lignosellulose Waste as a Xylanase Production Media of Mold Isolated from Rice Straw of Coastal-field

Utarti, Esti, Siswanto, S.

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Hemicellulose is one of lignocellulose waste component, so that xylanase is one of importance enzyme of lignocellulose waste biodegradation. Molds as main decomposer lignosellulose waste has enzyme activities higher than yeast and bacteria. The aim of the research is to find mold that have xylanolitic activity using lignocellulose waste as media production. The research consist of isolations and screening mols from coastal-field of watu Ulo Jember, xylanase production using lignocellulose waste and idntification of mold which has the highes xylanase activity. A total of 66 molds isolated from rice straw in coastal-field of Watu Ulo Jember. There were screened for their xylanase activity. In semiquantitatively screen on Oat Spelt Xylan plate, the result showed that 62 have xilanolytic activities. Based on clearing zone production, isolates ESW A1 (3.2), ESW A5 (3.1), ESW C 16 (3.26), ESW D4 (3.0) and ESW D15 (3.21) have xilanase activity index higher than others. Furthermore, quantitative analysis using wheat bran, rice straw and baggase in basic salt Mandel’s modification media showed that xylanase activity of isolate ESW D4 was higher on rice straw 3% as substrate production with activity 2.66 U/mL. Isolate ESW D4 identified as Aspergillus foetidus so that called as Aspergillus foetidus ESW D4. Keywords: rice straw, coastal-field, Aspergillus foetidus ESW-D

The Relationship of Sanitation Hygiene of Grilled Sausage at Car Free Day (CFD) Malang to Bacteria Colonies Number

Hariyati, Nabila, Budiyanto, Moch. Agus Krisno, Husamah, H.

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The number of bacteria in the grilled sausage is affected by sanitation hygiene. Aspects of hygiene sanitation include food handlers, equipment, presentation, means of peddlers, and merchant centers. If these five aspects do not meet the standards then it can trigger the occurrence of food contamination by microorganisms. This study aims to determine whether there is a hygiene relationship sanitation traders on the number of bacterial colonies roasted sausages in the car free day (CFD) of Malang City. Data collection using observation sheet referred to Decree of Minister of Health, Number 942/MENKES/SK/VII/2003. Data in the form of hygiene sanitation score. Product moment correlation is used to analyze the presence or absence of relationship between variables. Result of research indicate that there is relation of sanitation hygiene of merchant to the number of bacteria in burning sausage in Car Free Day (CFD) Malang. Keywords: hygiene sanitation, grilled sausage, the number of bacteria

Ethnomedicine of Medicinal Plants By Batak Phakpak Subethnic in The Surung Mersada Village, Phakpak Bharat District, North Sumatera

Silalahi, Marina, Nisyawati, N., Walujo, Eko Baroto, Mustaqim, Wendy

Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The research was conducted the ethnomedicine of medicinal plants by ethnic Batak Phakpak, in the Phakpak Bharat District, North Sumatra. The research was conducted by ethnobotany approach through semi-structured interview. A total of 39 respondents consisting of 7 key informants and 32 general respondents were interviewed. The data obtained were analyzed using values of use values (UV) and cultural index significance (ICS). The sub-ethnic of Batak Phakpak in Surung Mersada village have been used 128 species belonging 102 genera and 51 families to cure the 24 types of diseases. Asteraceae, Zingiberaceae, Euphorbiaceae, Fabaceae, Solanaceae, and Rutaceae are the families with the highest number of species, 12, 8, 8, 8, 7 and 6 species respectively. UV of medicinal plants utilized by Phakpak Batak sub-ethnic is 0.25-3.97, while ICS value is 3-150. The bark of Bischofia javanica as a cholesterol drug and the leaves of Sauralia pendula as a hypertension drug arethe plants that have the potential to be developed commercially, so its take research of the secondary metabolites and bioassay.Keywords: Bischofia javanica, Sauralia pendula, use values, cultural significance index