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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
ISSN : 26846748     EISSN : 26569825     DOI : -
Core Subject : Health, Science,
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 12 Documents
ANALYSIS OF APO-B SERUM LEVELS IN BALB/C MICE HYPERCHOLESTEROLEMIC AGAINST TEMULAWAK EXTRACT (Curcuma xanthoriza Roxb) Solihah, Riyadatus; Haris, M. Shofwan; Abror, Yogi Khoirul
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI

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Abstract

APO–B Serum levels is the most predictive value for the incidence of atherosclerosis and coronary heart disease. Curcuma xanthorrhiza Roxb contains curcumin, which can be used as an antioxidant, anti–inflammatory and antihypercholesterol. The mechanism of curcumin contained in ginger to reduce cholesterol is due to its function as a cholagoga or bile stimulant. This study aims to determine the effect of temulawak extract on the levels of APO–B Serum in hypercholesterolemia mice. This research were a true experimental study with a post–test only control group design carried out in February 2018. The extraction As much as 25 mice were divided into 5 groups where are group consisted of 5 mice. Positive control group (K+) were treated with high cholesterol feed and water, negative control group (K–) were given standard feed and water, treatment group 1 (P1) were given high cholesterol food and 25mg/kg BW of curcuma extract for 14 days, treatment group 2 (P2) were treated with foods high in cholesterol and 50mg/kg BW of curcuma extract for 14 days and treatment group 3 (P3) treated with high cholesterol and ginger extract 75mg/kg BW for 14 days. Examination of APO–B levels were measured using the spectrophotometric method. Data were analyzed using One–Way Anova. The results showed that the average of APO–B level at (K+) was 209.7 ± 1.02 mg / dL, at (K–) 115.3 ± 1.04 mg / dL, at (P1) 180.4 ± 1.07 mg / dL, at (P2) 147.6 ± 1.12 mg / dL, at (P3) 119.1 ± 1.10 mg / dL. Based on the results of statistical test it was found that there was a significant decrease in APO–B levels with p–value= 0.001 at alpha 0.05 (p <α).
THE EFFECT OF TEST TUBE STERILIZATION FROM SERUM LIPEMIC AGAINST LEVELS OF TRIGLYCERIDE GPO-PAP METHOD Fadhilah, Fitri; Sari, Ana Bina; Aprilianti, Astika
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
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Abstract

In terms of analytic factors, it is important to define acceptable levels of common interferences, such as lipemia or hemolysis. For triglyceride, the laboratory technician must define whether samples with excess lipemia will be included in the study; this depends, in part, on whether the interferences affect the methods. In most laboratories, glass or plastic that is in direct contact associated with bio hazardous material is usually disposable. If not, it must be decontaminated according to appropriate protocols. Immediately rinsing glass or plastic supplies after use, followed by washing with a powder or liquid detergent designed for cleaning laboratory supplies and several distilled water rinses, may be insufficient. To ensure that all remaining fat from lipemic serum that attached to the tube wall has been removed, then the sterilization process is carried out so that a sterile tube is obtained. The purpose of this study is to determine the effect of test tube sterilization from serum lipemic against levels of triglyceride GPO-PAP (Glycerol-3-phosphate oxidase-p-aminophenazone) examination. This research method was a laboratory experiment. We used 8 times repetition with tubes used first are given liquid fat and cleaned by sterilization, washed with surfactant and washed with water only. By using statistical tests ANOVA obtained of this study showed results p>0.05 which is mean the treatment that used did not show a significant difference in the treatment of ordinary water-washed tubes with sterilized tubes and surfactant washed tubes. The conclusion of this study is cleaning of the test tube with the sterilization method is recommended because to avoiding the fear of remaining pollutants that can affect the results, It can also minimize the life of bacteria and viruses from the sample to be examined. However, if the sterilization method is difficult to do because of limited equipment and so on, the use of surfactants and the correct method of cleaning the tube is enough to remove impurities such as fat.
EFFECT OF STARCH AND SULFURIC ACID ON DETERMINATION OF VITAMIN C IN PAPAYA FRUIT USING IODIMETRI Ngibad, Khoirul; Pradana, M Sungging; Y, Ingrid Retno
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
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Abstract

Vitamin C is an antioxidant that can be used to inactivate oxidation reactions and prevent the formation of free radicals. Sources of vitamin C are fruits, such as papaya fruit. The purpose of this study was to determine the effect of the indicator volume of 1% starch and 2 N sulfuric acid on the determination of vitamin C in papaya fruit samples. This study used the iodimetri method with a standard iodine solution, starch indicator and the addition of sulfuric acid. The variations of starch indicator volume include: 0.25; 0.5, 1, 2 and 3 mL and the variations of sulfuric acid volume include: 0, 2, 4, 6, and 8 mL. The results showed that the optimum 1% starch indicator volume was 1 mL and the optimum volume of 2 N sulfuric acid was 2 mL.    
CORRELATION OF MALNUTRITION, WORM INFECTION, PARENTS, INCOME AND KNOWLEDGE ON ANEMIA PREVALENCE AMONG 6-9 YEAR OLD STUDENTS OF LILIBA INPRES ELEMENTARY SCHOOL Tangkelangi, Marni
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
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Abstract

Anemia in school–age children will not only cause harm to health but also will have impact on students learning achievement. Thus, anemic children will indirectly affect the national development. The aim of this study is to observe the correlation between malnutrition, worm infection, parents income and knowledge on anemia prevalence among 6–9 years old children. The method of this research was a cross sectional study with a simple random sampling technique, consisted of 222 participants from Liliba Inpres Elementary School. This study was done by measuring children Body Mass Indeks (BMI) ––age to measure the z score and compare to WHO Children growth standard, by using microscope examination with direct method for identifying helminthiasis, by using questionaries to measure the parent knowledge and parent income and also by measuring haemoglobin values using POCT Device. The results showed that there are correlations between malnutrition and worm infection on anemia prevalence (p value 0.000). However, there are no correlations between parent’s knowledge (p value 0.469) and parent’s income on anemia prevalence among 6–9 years old children on Liliba Inpres Elementary School (p value 0.606). In conclusion, these findings confirm that malnutrition and worm infection was correlated with anemia prevalence on Liliba Inpres Elementary School Students so that they are advised to manage their nutritional intake and to practice personal hygiene.
SHAKING RED CAP-BLOOD COLLECTION TUBE WITHOUT ADDITIVE SUBSTANCES IS RECOMMENDED TO ACCELERATE THE BLOOD CLOTTING PROCESS Nugraha, Gilang; Rohayati, Rohayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
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Abstract

Many blood collection tube manufacturers do not recommend shaking the red tube. Shaking the red tube to avoid the intensity of the changed of blood that interacts with the glass surface of the tube will trigger the contact path in the coagulation cascade. Generally, the blood takes 30-60 minutes to clots at room temperature without shaking. The aim of this study was to determine the effectiveness of shaking the red-cap blood collection tube in producing serum volume. The method of this study was experimental As much as 5 mL of blood was taken and put into 3 tubes with a volume of 1mL each tube. The first and the second tube were shaken 8 times. The first tube was incubated for 10 minutes while the second tube was incubated for 25 minutes. Meanwhile, the third tube (as a control) was not shaken but was incubated for 40 minutes. The tube were centrifuged at 3000 g for 10 minutes. The serum volume was measured using micropipette and collected into Eppendorf tube. The results showed that there were a difference in the number of serums formed after tube shaking by time variation (P = 0.002), the results of the Post Hoc Test using Bonferroni test while showed that the second tube did not have a difference in serum volume with control (P = 0.751). It can be concluded that the red-cap blood collection tube, which was shaken 8 times and incubated for 25 minutes long could accelerate the coagulation process.
MICROBIAL PATTERN OF DIABETIC FOOT ULCER PATIENT IN JEMURSARI ISLAMIC HOSPITAL SURABAYA PERIOD 2012-2016 Donastin, Adyan; Aisyah, Aisyah
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
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Diabetic foot ulcers (DFU) are complications in people with diabetes mellitus (DM) in the form of wounds or tissue damage resulting in vascular insufficiency and or neuropathy that can develop into an infection. Early detection of germs of diabetic foot ulcers may be used as a recommendation of empirical therapy before the definitive treatment based on culture results and appropriate antibiotics treatment, which may reduce hospitalization time and amputation events. According to Riskesdas in 2013, state that the number of antibiotic used without prescriptions in Indonesia about 86.1%. The study aims to retrospectively analyze the bacterial culture and drug susceptibility test results for patients with diabetic foot ulcers (DFU) in Jemursari Islamic Hospital Surabaya during 2012–2016 to help clinicians choose a more appropriate empirical antibiotic treatment for DFU. This study used cross–sectional designed with retrospective approaches, which analyzed descriptively and samples were taken by the total sampling of 11 samples. This research was conducted at Islamic Hospital of Jemursari Surabaya in May–September 2017 by using medical record data which are outpatient and inpatients who treatment at Jemursari Islamic Hospital. The result was found 6 types of bacteria consisting of Staphylococcus aureus (18%), Staphylococcus non–haemolytic (18%), Klebsiella pneumonia (27%), Enterobacter aerogenes (18%), Burkholderia cepacia (9%), Escheria coli (9%). The most sensitive antibiotics in the Gram–positive bacteria in this study are Amikacin, Teicoplanin and Oxacillin and the most resistant to Amoxicillin and Ampicillin whereas the most sensitive antibiotics in the Gram–negative bacteria in this study were Meropenem and the most resistant to Ciprofloxacin and Trimethroprim–sulfamethoxazole.
COMPARISON OF GLUCOSE REDUCTION IN URINE USING BENEDICT METHOD HEATED BY METHYLATED FLAME WITH 100°C WATERBATH Fadhilah, Fitri; Vanawati, Noviana
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1075

Abstract

The high prevalence of Diabetes Melitus (DM) is a global problem that must be solved by health workers around the world. This study aims to determine the differences in the results of urine reduction examination using benedict method heated by spirtus flame and waterbath 100°C. This research method is a laboratory experiment. The results of this study indicated that the urine reduction examination by heating methylated flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the methylated flame and waterbath 100oC did not affect the results. However, there are a difference in the process of urine reduction examination by heating flame which was need a longer time up to 3-5 minutes. Additionally, the solution in the tube could be exploded. It was also time consuming which could only carry out one by one sample. Meanwhile, the heating of urine by using 100oC waterbath is relatively faster, which only took 2 minutes. The urine was not also exploded when it was boiled and the heating process could perform 6-8 samples at the same time (depending on the tube rack). In conclusion, the heating method of urine by using waterbath was better than spirtus (methanol) flame since it could carry out large sample in one time and it was safer for the laboratory personnel.
N-TERMINAL PRO-BRAIN NATRIURETIC PEPTIDE (NT-proBNP) IN STAGE 1 AND STAGE 2 HYPERTENSION PATIENTS Djami, Supriati Wila
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1159

Abstract

The increased levels of NT-proBNP in the blood occur when heart function, especially the left ventricular muscle chambers of the heart increases. Therefore, NT-proBNP is used as a biomarker to detect heart failure.The level of N Terminal ? Pro Brain Natriuetic Peptide was independently associated with an increased risk of hypertension. This study aimed to determine the difference of NT-proBNP serum levels and the correlation between the levels of NT-proBNP in patients with stage 1 and stage 2 hypertension. This research was conducted at RSUP dr. Wahidin Sudirohusodo in August - September 2018. The study used a cross-sectional design with the total of 72 hypertensive patients, who had met the inclusive criteria. NT-proBNP levels were measured using the ELISA (Enzyme Linked Immunosorbent Assay) method.  The collected data was processed using Mann Whitney Different Test and Spearman's rho Correlation Test. The study results indicated that the level of NT-proBNP in the hypertensive patients with stage 2 was higher and significantly different (p = <0.001) compared to stage 1 hypertensive patients. NT-proBNP levels were higher in the hypertensive group of >6 years than in the hypertensive group <6 years. There were significant differences between the two groups statistically (p=0.010). It can be Conclude that there is a significant difference in the levels of NT-proBNP with a degree of hypertension where NT-proBNP levels were higher in patients with stage 2 hypertension compared to stage 1 hypertension, although  there was not statistically significant correlation between levels of NT-proBNP with Hypertension degree. Further research was needed to determine the relationship of NT-proBNP levels with the degree of hypertension, which can confirm the diagnosis, especially in patients with hypertension. Also, it is suggested to consider the accuracy of the data length of a patient suffering from undiagnosed hypertension.
THE EFFECT OF BEETROOT (Beta vulgaris L.) JUICE ON CHOLINESTERASE ACTIVITY IN FARMERS EXPOSED TO ORGANOPHOSPHATE PESTICIDES Wulandari, Devyana Dyah; Santoso, Andreas Putro Ragil; Wulansari, Devyani Diah
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1215

Abstract

A decrease in cholinesterase (chE) enzyme activity is an indicator of pesticide poisoning, especially organophosphate pesticides. The chE activity reduction will result in an increase in the amount of acetylcholine and will bound to muscarinic and nicotinic receptors in the central and peripheral nervous system which can lead to the onset of Alzheimer's disease. However, antioxidant products can slow the progression of Alzheimer's disease by protecting neurons from oxidative stress. Red Beetroot (Beta vulgaris L.) contains betalains and phenolic compounds which act as an antioxidants that are capable of preventing such illness. This study aims to prove the concept that antioxidant compounds in red beetroot juice can increase the chE activity in subjects exposed to organophosphate pesticides. This research is an observational study with an experimental approach. This research used 25 farmers who were exposed to organophosphate pesticides as respondents. They were given 500 ml of beetroot juice twice a day for 2 consecutive months. Cholinesterase levels were measured before and after consuming red beet juice. Measurement of cholinesterase levels was carried out using a kinetic photometric test. This method was developed based on recommendations from the German Society of Clinical Chemistry (DGKC). The results showed that the average of chE (U/L) Level Before Treatment was 8.102 and 8.380 after treatment with a p value 0,62. It can be concluded that there was an increase in the level of cholinesterase but it was not statistically significant. This may be caused by a different response to activity of cholinesterase after consuming beetroot juice in each subjects, age difference, smoking habits, and personal protective equipment.
IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY Riscahyani, Nosy Maria; Ekawati, Evy Ratnasari; Ngibad, Khoirul
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1291

Abstract

Ascorbic acid mostly comes from vegetables and fruits, especially fresh fruits. Vitamin C is a vitamin that can be formed by several types of plants. One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues. The purpose of this study was to determine the levels of vitamin C contained in papaya using iodimetry and UV-Vis spectrophotometry. The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I2 until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H2SO4 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was 0.1313%. In conclusion, vitamin C levels analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.

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