Serambi Biologi adalah sebuah jurnal yang mempublikasikan hasil penelitian ilmiah, short communication dan artikel review. Artikel ilmiah yang dipublikasi adalah artikel dalam bidang biologi (biodiversitas, biosistematika, ekologi, fisiologi, genetika dan bioteknologi, biokimia) yang meliputi semua bentuk mahluk hidup mulai dari mikroba, fungi, tumbuhan, hewan, manusia dan virus. Serambi Biologi dipublikasi oleh Universitas Negeri Padang dimana setiap tahunnya akan terbit sebanyak 4 kali. Penerbitan pada jurnal ini tidak dikenakan biaya.
Primers are oligonucleotides which have an important role in the PCR process. To get the primer can be done by doing a primary design. Beta-carotene encoding genes are genes that play a role in the formation of beta-carotene in carotenoid biosynthetic pathways in several plants including cassava. This study aims to obtain primers who can identify beta-carotene encoding genes in cassava plants. Primers are designed using primary 3 plus software online. The results obtained were five specific primers, namely PSY, CRTISO, LCY α, LCY β, and BCH primers which could identify beta-carotene coding genes such as PSY, CRTISO, LCY α, LCY β, and BCH on cassava. Primers designed according to the criteria for primary length, GC content and temperature melting for beta-carotene encoding genes in cassava.
Background this study was to examine the influence of extract of bitter against implantation and ratio of male female in mice. The purpose of this research is to know the influence of extract of bitter (Andrographis paniculata Ness.) against implantation and ratio male female mice (Mus musculus L.).This type of research is research experiments using Completely Randomized Design (CRD), which consists of 4 treatments and 6 replicates. This research procedure is the preparation of the implementation phase, research and observations. Data were analyzed with ANOVA (analysis of Variance) and if the results of the data analysis shows the influence of then continued with test List Significant Different (LSD) on p<0.05.The results showed that the sambiloto extract could reduce the number of implants, the number of birth fetuses, and the ratio of female tillers but did not cause a decrease in the number of corpus luteum, body weight, and ratio of male mice (Mus musculus L.). the ratio of male and female tillers is different because the female fetus has a weaker survival compared to the male, so the sambiloto content causes a decrease in the number of female tiller ratio.
Abstract. Petai plants (Parkia speciosa Hassk.) Are thought to originate from Malaysia. The most important part of the petai plant to use is the seeds. Petai seeds contain hexathionine, tetrathiane, trithiolane, pentathiopane, pentathiocane, and tetrathiepane which cause the petai to emit a smell of smell. One way to reduce the smell of smell in petai is to do fermentation. Benefits of fermented food products are for preservation, enhancing taste, and for producing new products. This study aims to find out whether from color, texture, aroma can be accepted by the community. This research was conducted at the Research Laboratory and Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University from October 2018 to March 2019. The data of this study were obtained from questionnaires tested using hedonic tests (preferences) and presented in the form of percentages. The results of this study showed that for color 74% while the criteria did not like 64%, for texture as much as 72% of those who liked and who did not like 52%, and for aroma 58%, while those who did not like 46%, for flavor taste of tempai petai seeds 66% while those who don't like 32%. The physical characteristics of tempeh are in accordance with the criteria of SNI 3144: 2009, namely: having a normal odor (typical tempe), normal color (white), compact texture, and normal taste (typical taste of tempe and not foreign).Keywords: Petai, Organoleptic, Fermentation
Sclerotium rolfsii is a fungus that has a wide range of host and high pathogenicity that can cause stem rot, root rot, and wilt in plants. Diseases caused by S. rolfsii can be overcome by using synthetic or chemical fungicides. The use of synthetic or chemical fungicides on an ongoing basis can have a negative impact on human health. Natural pesticides derived from plant leaf extracts are explored to treat plant diseases. One of them is Hyptis suaveolens (L.) Poit. The compounds contained in this plant are alkaloids, flavonoids, tannins, and suaveolic acid. This study aims to examine the effect of H. suaveolens (L.) Poit. extract and the most effective concentration in inhibiting the growth of S. rolfsii. H. suaveolens (L.) Poit. leaf extract was prepared by maceration method using 96% ethanol. The leaf extract solution was evaporated using a vacuum rotary evaporator. The concentration used is 0% (control), 5%, 10%, 15%, and 20% with 4 repetitions. The data obtained were analyzed using one way ANOVA and Duncan test. The results showed that H. suaveolens (L.) Poit. leaf extract could inhibit the growth of S. rolfsii and the most effective concentration was at concentration 15% with 56% inhibition percentage.
Cases of bacterial resistance to antibiotics are a serious problem in the world of health. Exploration to find new sources of antibiotics needs to be done, one of them is by utilizing endophytic bacteria Andaleh. The purpose of this study was to amplify the 16S rRNA coding gene, three isolates of Andaleh endophytic bacteria producing antimicrobial compounds with ATB A4.1, B.J.T.A.2.1, B.J.T.A.3 isolate code. This research is a descriptive research, carried out from November 2017 to June 2018 at the Research Laboratory of the Biology Department FMIPA UNP. The results showed that the amplification of the 16S rRNA gene from the three isolates succeeded in producing a single DNA band located between DNA bands 2027bp and 564 bp from the marker λ HindIII.
Background this study was to examine the influence of extract of bitter against levels of uric acid in mice. The purpose of this research is to know the influence of extract of bitter (Andrographis paniculata Ness.) against the levels of uric acid house mouse (Mus musculus l.) males. This type of research is research experiments using Completely Randomized Design (CRD), which consists of 4 treatments and 5 replicates. This research procedure is the preparation of the implementation phase, research and observations. This research procedure is the preparation of the implementation phase, research and observations. Observation data were analyzed by ANOVA (Analisis of Varians) test and if there were significant differences then continued with the DNMRT (Duncan New Multiple Range Test) further test on significance 5 %. The results showed that the levels of uric acid house mouse (Mus musculus l.) males at the basal data changes after the and before the given sambiloto. The male MICE are naturally conditioned in a State of hiperurisemia with penginduksian compound containing high levels of purin. Based on the results of the study it can be concluded that the administration of bitter extract (Andrographis paniculata Ness.) significantly affected the decrease of uric acid levels in male mice (Mus musculus L.) in treatment 3 (P3) at a dose of 0.6 mg / BB mice.
ABSTRAK Threo Wanda Marten. 2018. Pengaruh Sumber Mineral dan Jenis Isolat Dari Pseudomonad Fluoresen Terhadap Produksi Siderofor Pseudomonad fluoresen merupakan agen hayati yang dapat diisolasi dari daerah permukaan akar tanaman. Kelompok bakteri dapat menghasilkan senyawa siderofor. Siderofor merupakan senyawa yang memiliki afinitas besi yang sangat tinggi, larut dalam air dan cepat berdifusi. Produksi siderofor tersebut tergantung pada nutrisi yang terdapat pada dalam media tumbuh.Penelitian ini bertujuan untuk menentukan sumber mineral terbaik untuk produksi siderofor dari isolat pseudomonad fluoresen. Penelitian ini adalah penelitian eksperimen. Menggunakan rancangan acak lengkap faktorial (RALF) dengan dua faktor (jenis isolat dan sumber mineral) dan 3 kali ulangan. Isolat yang digunakan PfPj1 dan PfKd7 sedangkan sumber mineral yaitu Zn dan Fe. Deteksi siderofor diukur menggunakan spektrofotometer pada panjang gelombang 410 nm. Data yang diperoleh dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji lanjut DNMRT dengan taraf nyata 0,05.Hasil penelitian didapatkan kedua Isolat yang ditumbuhkan pada medium dengan penambahan mineral menghasilkan produksi siderofor yan tidak berbeda nyata. Mineral yang ditambahkan kedalam medium tumbuh kedua isolat menghasilkan kandungan siderofor yang berbeda. Interaksi antara isolat dengan sumber mineral memberikan pengaruh yang nyata terhadap produksi siderofor dari pseudomonad fluoresen. Kata Kunci : pseudomonad fluoresen, sumber mineral, siderofor
Endophytic bacteria can produce active compounds that function as antitumor, antioxidant and antimicrobial. The steps taken can be done for the use of endophytic bacteria is identification of bacteria. Endophytic bacteria have a 16S rRNA gene. The most widely used technique for identification is DNA sequencing. For the sequencing process it takes a primer that can read 1500 bp sequences. The purpose of this study was to obtain primers who could read the internal area of the 16S rRNA gene and be able to be used in the sequencing process. This research uses BioEdit software and Primer Designer. The isolate used as a DNA template in the sequencing process is B.J.T.A.2.1. DNA template were amplified using the PCR method. The results of PCR products were analyzed using 1% agarose gel electrophoresis. The sequencing process is carried out with NIF (forward) and NIR (reverse) primers. The results showed that the primer could recognize the 16S rRNA internal region of the antimicrobial compound endophytic bacteria. Both primers are able to start the sequencing process and produce a sequence of 1130 bp. However, good sequence readings were only ± 100 bp due to contamination of DNA template.
Catfish (Clarias gariepinus L.) is a type of consumption fish from fresh water with elongated body and slippery skin. Efforts to increase catfish cultivation are characterized by high stocking density followed by increased use of artificial feed that is rich in protein. Catfish disease is one of the problems that are often encountered in the effort to enlarge catfish. Therefore, an alternative cure is used with areca leaf liquid fermentation (Areca cathecu L.) and surian leaves (Toona sinensis ROXB.). This study was an experimental study, using a factorial complete randomized design consisting of two factors, namely the dosing factor (A) and the length of the observation day (B). Each treatment was repeated 6 times. The data of this study were analyzed using Analysis Of Variants (ANOVA), the results were significantly different continued using the DNMRT advanced test with a level of 5%. Based on the research conducted, it can be concluded that the use of areca leaf (Areca cathecu L.) and surian leaf (Toona sinensis ROXB.) liquid fermentation influences the wound healing in catfish (Clarias gariepinus Var.) and the most optimal dose to assist the healing process is at a dose of 15 ml.
Drought-tolerant hybrid rice breeding certainly requires parental lines who are also tolerant to drought. The selection of superior rice elders usually comes from local rice. Polyetilene glycol (PEG) has been widely used as an osmotic solution to detect plant drought tolerance in the germination phase, especially PEG 6000. This study aims to determine the effect of PEG 8000 on rice germination and find out local drought tolerant rice varieties using drought susceptibility index (DSI) values through several germination variables, namely germination percentage, shoot length and seminal root, weight of shoots and seminal roots. The experiment was conducted at the Integrated Research Laboratory, Biology Department, FMIPA State University of Padang, using factorial randomized block design with 2 factors 3 replications with the first factor being 6 rice varieties while the second factor was 3 levels of PEG 8000 concentrations (PEG 8000 0%, 10% and 20%). If the DSI value is ≤ 0.5 then the variety is tolerant, if 0.5 1.0 then the variety is intolerant (sensitive). The classified tolerant variety is Harum and Baroto; while sensitive varieties is Keriting and Batang Palo. The classified moderat variety is Situbagendit and Randam Kaus.