Bioscientia Medicina : Journal of Biomedicine and Translational Research
Published by Universitas Sriwijaya
ISSN : 25980580     EISSN : -
BioScientia Medicina is an open access international scholarly journal in the field of biomedicine and translational research aimed to publish a high-quality scientific paper including original research papers, reviews, short communication, and technical notes. This journal welcomes the submission of articles that offering a sensible transfer of basic research to applied clinical medicine. BioScientia Medicina covers the latest developments in various fields of biomedicine with special attention to medical sciences, Traditional Herb, genetics, immunology, environmental health, toxicology, bioinformatics and biotechnology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health.
Articles 36 Documents
The Status of Oxidants and Antioxidants in Children with Nephrotic Syndrome

Lestari, Hertanti Indah, Zulissetiana, Eka Febri, Melizah, Ardesy

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v2i1.35

Abstract

Background: The nephrotic syndrome (NS) resistance and relapse to treatment pose challenges in the management of NS. Several experimental studies on both animals and humans have assessed the association between NS and the balance between the oxidants and anti-oxidants. The study aims to compare the status of oxidants and anti-oxidants of NS patients between the massive proteinuria, the remission, the steroid resistance and the control groups. Methods:  a cross-sectional design to assess the status of oxidants and antioxidants in children with the nephrotic syndrome. The eligible subjects were divided into four groups, the massive proteinuria group, the remission group, the steroid resistant group and the control group. The status of oxidants and anti-oxidant were evaluated with the Malondialdehyde (MDA) and the Total Antioxidant Status (TAS), respectively. Results: The highest mean MDA levels was observed in the steroid resistant group followed by the massive proteinuria group and the remission group. The mean MDA level of the proteinuria group (massive proteinuria and steroid-resistant) is higher than the remission group. The mean TAS levels in the remission group were higher than the massive proteinuria group, but the difference was not statistically significant. Moreover, the mean difference of SAT between the proteinuria group and without proteinuria was not statistically significant. Conclusions: The oxidative stress marker (MDA) was higher in the NS patients with proteinuria than the patients without proteinuria. The difference in the total anti-oxidant status in NS patients with massive proteinuria, remission and steroid resistance were not statistically significant.   Keywords: Nephrotic syndrome, oxidative stress, anti oxidant 

Sleep and Bone Density: A Study on Postmenopausal Indonesian Women

Irmayati, Shafira, Reagan, Muhammad, Legiran, Legiran

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 3 No 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v3i1.68

Abstract

Background: Low bone density has been known as one of the factors that increased fracture risk. Aging, estrogen deficiency, low BMI, and inadequate calcium intake are known factors that contribute to decreased bone density. Other than the known factors, some research show that sleep duration can also lower bone density. Sleep affects bone density in a way that it causes increased level of cortisol, proinflammatory cytokine, and decreased in physical activity. Method: This study is an observational analytic study with cross-sectional design. It is conducted on menopausal patients that has been examined with bone mineral densitometry at Poliklinik Reumatologi Penyakit Dalam RSUP Dr. Mohammad Hoesin Palembang in November 2017 to Oktober 2018. Samples are collected using total sampling technique. Results: From 93 subjects that participated in this study, 20,4% of them had osteopenia, and 51,6% had osteoporosis. Patients with low bone density, mainly slept for 6 to 8 hours at night per day, 30 minutes of nap per day, and has a total sleep duration for less than 8 hours each day. This study shows no significant association between night-time sleep, daytime napping, and total sleep duration with bone density (p value = 0,168, p value = 0,831, p value = 0,984). Analysis on other risk factors show significant association between body mass index and low bone density (p value = 0,002). Conclusion: There are no significant association between sleep duration and bone density in menopausal patients at Poliklinik Reumatologi RSUP Dr. Mohammad Hoesin Palembang.

Antifungi Effect of Keghenyat Leaves Fraction (Acmella uliginosa) In Vitro Study

Hijir, Agustina Septi, Salni, Salni, Marwoto, Joko, Triwani, Triwani, Nita, Sri, Saleh, Mgs Irsan, Maritska, Ziske, Parisa, Nita, Lusiana, Evi, Tamzil, Nia Savitri, Rosdah, Ayeshah Augusta

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 1 No 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v1i1.5

Abstract

Introduction Candida is a yeast fungus classes most commonly found in the oral cavity, gastrointestinal tract, reproductive tract and skin, especially the species Candida albicans. Alternative medicines such as herbs are easily available and affordable by the community, such as keghenyat leaves (Acmella uliginosa). Aim of StudyTo determine the activity of Keghenyat leaves fraction (Acmella uliginosa) compared to Nystatin against Candida albicans in vitro. MethodsIn vitro study was held in Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Science and Medical Faculty, Sriwijaya University in December 2015 and January 2016. Research stages: extraction, fractionation, sensitivity test using Nystatin and Candida albicans, fractions antifungal activity test, the determination of minimum inhibitory concentration (MIC), bioautografi test and determination of compound classes. One final stages of testing the active fraction Keghenyat leaves (Acmella uliginosa) with Nystatin. Data were analyzed using ANOVA and Post Hoc Duncan test and linear regression using SPSS 20. ResultsHexane faction of Keghenyat leaves (Acmella uliginosa) is active against the fungus Candida albicans, has a Minimum Inhibitory Concentration (MIC) of 0.625% (6250μl) against the fungus Candida albicans antifungal contain active compounds that terpenoids and flavonoids, amounting to 84.68 mg / ml equivalent to 1 mg / ml Nystatin against the fungus Candida albicans. ConclusionThere is a significance different MIC between hexane fraction of Keghenyat leaves (Acmella uliginosa) and Nystatin, Nystatin have more high activity.

The Efficacy of Sauropus androgynus Leaves Extract To Improve Cognitive Function in Wistar Rats Induced Alzheimer’s

Hidayat, Rachmat, Adelia Safitri, Raden Ayu, Umar, Tungki Pratama, Maretzka, Arindi

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 3 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v2i3.61

Abstract

Background Sauropus androgynus leaves is the substance which has the potency to prevent degenerative processes. Sauropus androgynus leaves has flavonoid as the main component. Flavonoid has antioxidant and anti neuro-inflammation that can be used to prevent Alzheimer dementia. Research purpose is knowing the effect of Sauropus androgynus leaves for the cognitive function and β-amyloid expression in the hippocampus of wistar rats. Methods Reseacrh was done by in vivo method, where male wistar rats (n=24) were distributed to six groups which consisting of four rats. Group 1: Normal control, group 2: positive control, group 3: standard treatment (B12 vitamin), group 4, 5 and 6 were give Sauropus androgynus leaves extract with the dose of 75 mg/kgBB, 150 mg/kgBB and 300 mg/kgBB, respectively for 28 days. Cognitive function was evaluated by t-maze test, where hippocampal β-amyloid expression was tested by immunohistochemistry. Results Time differences (day 0-28), alternation ratio distinction (day 0-28) and β-amiloid expression were: group 1 (1,84 second; 0,23 unit; 0,518%), group 2 (56,78 second; -0,42 unit; 40,036%), group 3 (34,46 second; -0,25 unit; 33,08%), group 4 (32,83  second; -0,09 unit; 28,88%), group 5 (-3,91 second; 0,42 unit; 14,728%), group 6 (24,25 second; 0,42 unit; 9,4%). Conclusion Sauropus androgynus leaves extract at the dose of 150 mg/kgBB and 300 mg/kgBB can maintain cognitive function by decreasing hippocampal β-amyloid formation.

The Efficacy of Red Ginger Fraction (Zingiber officinale Roscoe var. rubrum) as Insecticidal Aedes aegypti

Anwar, Chairil, Syukur, KM Yahya, Dalilah, Dalilah, Salni, Salni, Novrikasari, Novrikasari

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v2i2.40

Abstract

Aedes aegypti is a vector of Dengue Hemoragic Fever. The use of synthetic chemical insecticide is the most common way to control Ae. aegypti. However, since it can cause resistency if it is used in a wide scale with high frequency and continously, it requires an alternative way. Red ginger (Zingiber officinale Roscoe var. rubrum) is one of natural insecticide which is safe to be used to control Ae. aegypti. This research aims was to determine the activity of active fraction of red ginger to mortality of Ae. aegypti. It was a quasi-experiment research with post test only control design. Twenty Ae. aegypti Liverpool strain were the sample of this research with 5 concentration for each and 4 times repetition. The result showed that concentration 0.5%, 1.0%, 1.5%, 2.0% and 2.5% causing mortality percentage of 32.5%, 33.8%, 51.3%, 58.8% and 65.0% respectively. One way Anova test showed p-value < α (0.00 < 0.05). It was concluded that there was a significant difference of various concentration of active fraction n-heksan of red ginger extract to the mortality of Ae. aegypti. The lethal concentration (LC50) was 2.409%. It was suggested to continue this research with reppellant or bio-assay test method.   Key words: Toxicity, extract, fraction, Aedes aegypti, Insecticidal

Antibacterial Efficacy of Aloe vera Sap Against Staphylococcus aureus and Escherichia coli

Azahra, Safira, Parisa, Nita, Fatmawati, Fatmawati, Amalia, Ella, Larasati, Venny

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 3 No 2 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v3i2.87

Abstract

Abstract Background Aloe vera is a plant that has been used as an alternative drug. This plant contains various compounds, like anthraquinone, saponin, flavonoid, alkaloid, and tannin that has an antibacterial effect against Staphylococcus aureus and Escherichia coli. Both of it were responsible for the infection incident. This study aims to determine the efficacy of Aloe vera sap as an antibacterial against Staphylococcus aureus and Escherichia coli. Methods An experimental study, in vitro using post-test only control group design, has been done at laboratory of Medical Faculty of Sriwijaya University, by examining the antibacterial activity of Aloe vera sap in five different concentration (5%, 10%, 20%, 40%, dan 80%) using well diffusion and solid dillusion method to determine the minimum bactericidal concentration (MBC). And then continued with the phytocemical screening to determine the compound inside the Aloe vera sap. Results Aloe vera sap were able to kill Staphylococcus aureus at 5% and Eshcerichia coli at 80%. Compatibility test showed that Aloe vera sap with concentration of 10%, 20%, 40%, and 80% are compatible with amoxicillin, therefore 80% is compatible with cefotaxime. This ability due to the compound that it contains, which is alkaloid, flavonoid, tannin, quinone, and saponin. Conclusion Aloe vera sap is effective as an antibacterial against Staphylococcus aureus and Escherichia coli.   Keyword: Aloe vera sap, antibacterial, efficacy, Staphylococcus aureus, Escherichia coli.  

Efficacy of Topical Coffee Paste to Collagen Density in Wistar Rats Incision Wound

Harahap, Debby Handayati, Argentina, Fifa, Kurniawati, Yuli, Murti, Citra Tresna, Satrio, Yudha Dwi

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v2i1.14

Abstract

Background :Collagen plays a very important role in humans at every stage of wound healing. Coffee contains many antioxidants that stimulate the formation of collagen dermis by increasing the production of Tissue Inhibitor of Matrix Metalloproteinas-1 in the dermis that serves to inhibit the breakdown of collagen. This study aims to determine the efficacy of topical pasta coffee to collagen density. Methods: Experimental Laboratories Study with pre and post test controlled group design research was undertaken from September to November 2017 at Animal House and Dyatnitalis Laboratory, Palembang. 30 male white rats of wistar strain were divided into 5 groups then performed 2 cm dermal incision and giving various concentration of coffee paste. On the day 0 and 5th after coffee paste, the percentage of collagen density is checked. The efficacy of coffee paste was analyzed by Paired T Test/Wilcoxon while the comparison of efficacy  was analyzed by Independent T Test / Mann Whitney then continued with One Way ANOVA Test and Tukey test. Data analysis using SPSS version 18.0. Results: There was an average difference of percentage level of collagen density before and after vitamin C (p = 0,003), coffee paste 20% (p = 0,043), coffee paste 40% (p = 0,045) and coffee paste 80% (p = 0,011) . In addition, there was an average difference of percentage of collagen density between negative control group with positive control group and 40% coffee paste. From the results of Post Hoc test results showed that there was a difference of percentage of collagen density of negative control group with coffee paste group 40%. Conclusion: There is efficacy of topical coffee paste on collagen density in various concentrations where the highest increase of collagen density is 246,96% after coffee paste 40%. Keyword: Coffee Pasta, Collagen, Injured Incision, Vitamin C

The Efficacy of Combination Extract Andrographis peniculata and Syzygium polyanthum on Glucose Uptake in Skletal Muscle in Diabetic Rats

Karim, Fatmawati, Panserga, Ester Gonzales, Saleh, Mgs Irsan

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 4 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v2i4.63

Abstract

Insulin resistance is impaired insulin signaling cascade in target cells to respond normal or elevated circulating insulin to the final cellular effect, such as translocation of vesicles containing GLUT4 glucose transporters, which is the major mediator of glucose removal from the circulation and a key regulator of whole-body glucose homeostasis. Among the several plants, Andrographis peniculata (sambiloto) and Syzygium polyanthum (Daun salam). In the present study, a combination was made, Andrographis peniculata (sambiloto) and Syzygium polyanthum (Daun salam), and to determine the effects of combination on tretament insulin resistance. Male Wistar rats (weight, 200-300 g) were randomized into five groups (6 rats/group). Group 1: negative group. Group 2: positive group (metformin 63 mg/kgBW ). Group 3,4 and 5: treatment with extract combination, each group 250 mg/kgBW, 500 mg/kgBW and 1000 mg/kgBW. Rats were induced by high fat diet-glucocorticoid for insulin resistance. Insulin and GLUT-4 were assayed by ELISA.  Treatment with extract combination (250, 500 and 1000 mg/kgBW) and metformin for 2 weeks showed a sigificant decrease in fasting plasma insulin compare with the negative control rats with a reduction of 11,2%, 33,6%, 20% and 19,4%, respectively.  Two weeks treatment either extract combination or metformin in diabetic rats, significantly increased GLUT 4 level (p<0,05) with a percentage increase of 6,68%, 15,21%, 12,76% and 1,77%.

The Effect of Active Gambir (Uncaria gambir) Fraction on TNF-α Protein Expression and Lesion Size in Rats Induced Gastritis

Suasti, Ni Wayan Lisa, Oktafitria, Lunsi, Aidia, Ellya, Dhani, Rahmad

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 2 No 3 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v2i3.53

Abstract

Background: Gambir (Uncaria gambir) is empirically used to treat abdominal pain and vomittus caused by gastritis because of its anti-inflammatory effects, especially flavonoid. This study aims to determine the effect of active gambir fraction on TNF-α protein expression and wound size in white rats gastritis model. Methods: The research method used experimental study design in vivo with post test with control group design. Rats were divided randomly in 11 groups and were induced to be gastritis for 1 day. Group 1 (negative control) was given aquadest of 5 mL, group 2 (positive control) was administered ranitidine 10 mg/kgBW, groups 3, 4, and 5 were given n-hexane fraction, groups 6, 7 and 8 were given a water fraction, and groups of 9, 10, and 11 were given ethyl fractions with each group receives dose of 20, 40, and 80 mg/kgBW and all groups were treated for 3 days. Rats were dissected on 5th day for examination of gastric mucosal lesion size and performed ELISA expression of TNF-α expression of gastric mucosal tissue. The results of this study were assayed by SPSS 18. Results: The result of the research using Kruskal-Wallis test showed that there were significant differences (p <0.05) of the lesions size between the sample groups where  control positive, ethyl fraction 20, 40, 80 mg/kgBW, and water fraction 20, 40 mg/kgBB had the gastric ​​mucosal lesion size differed significantly with the negative control group, while the TNF-α protein expression test  using Kruskal-Wallis showed that there was a significant difference (p <0.05) TNF-α levels of all groups against the negative control. Conclusion: Active gambir fraction had a potention to reduce size of mucose gaster lesion and reduce expression of TNF-α protein.   Keywords: Gambir – TNF-α – antiinflamatory – gastiritis

Association of Sociodemographic, Knowledge, Attitude and Practice with Pediculosis Capitis

Amelia, Linda, Anwar, Chairil, Wardiansah, Wardiansah

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 3 No 1 (2019): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Original Source | Check in Google Scholar | | DOI: 10.32539/bsm.v3i1.64

Abstract

Introduction : Pediculosis capitis is an infection of scalp and hair in humans caused by infestation of Pediculus humanus capitis and usually extends rapidly in a dense environment such as boarding schools. Aim of study : The aim of this study was to determine the association of students’ sociodemographic, knowledge, attitude and practice with pediculosis capitis in Pondok Pesantren Tahfidzil Qur'an Yayasan Tijarotal Lan Tabur Palembang. Methods : This research was an analytical observational study with a cross sectional design conducted on November 2018. There were 117 samples that had the inclusion criteria and hadn’t exclusion criteria. Data collected from anamnesis, questionnaires and hair examinations of respondents. The results were analyzed using Chi-Square and Logistic Regression. Results : Out of 117 students, 57 students (48.7%) has been found positive pediculosis capitis. Statistical test showed that  age (p<0.001), gender (p<0.001), education level (p<0.001), knowledge (p=0.035), attitude (p=0.003) and practice (p =0.043) have significant association to the prevalence of pediculosis capitis. However, there was no significant association between father's education (p=1.000), mother's education (p=0.743), father's job (p=0.314), mother's job (p=1.000) and parents' income (p=1.000) to the prevalence of pediculosis capitis. The results of Multiple Logistic Regression analysis showed that gender, knowledge and practice were the most influential factors to the prevalence of pediculosis capitis (p<0.05) with the probability of 96.7%. Conclusion : There was a significant association between age, gender, education level, knowledge, attitude and practice to the prevalence of pediculosis capitis, and the most dominant variables were gender, knowledge and practice.

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