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Journal of Indonesian Dental Association
The first edition of JIDA will be launched by Indonesian Dental Association (PBPDGI) on October 2018. JIDA, a biannually published scientific journal, is an open access, peer-reviewed journal that supports all topics in Oral and Dental Sciences, including to Biochemistry, Conservative Dentistry/Endodontics, Dental Material, Dental Radiology Microbiology, Histology, Oral Anatomy, Oral Biology, Oral Surgery, Oral Medicine, Oral Pathology, Orthodontics, Pedodontics, Periodontology, Pharmacology, Prosthodontics, and Public Health.
Articles
5
Articles
Titanium Dioxide Addition to Heat Polymerized Acrylic Resin Denture Base Effect on Staphylococcus aureus and Candida albicans

Putranti, Dwi Tjahyaning, Fadilla, Afrina

Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

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Abstract

  Introduction: Heat-polymerized acrylic resin is used to manufacture almost all denture bases. One of the disadvantages of acrylic resin is its porosity and surface roughness; food scraps attach easily. If the dentures are not cleaned, they become a place for microbial species development and cause denture stomatitis, with Staphylococcus aureus and Candida albicans as the major etiologic agents. Adding nanoparticles of titanium dioxide to heat-polymerized acrylic resin may provide antimicrobial activity to the acrylic resin base.   Objectives: This study aimed to determine whether adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base materials affected the amounts of Staphylococcus aureus and Candida albicans.   Methods: The samples in this study used heat-polymerized acrylic resin without the addition of TiO2 nanoparticles and with the addition of 2%, 3%, and 4% TiO2 nanoparticles on test bar sizes of 10 mm × 10 mm × 1 mm. There were 48 total test samples: 24 of Staphylococcus aureus and 24 of Candida albicans. The obtained data were analyzed by a one-way ANOVA test.   Results: The results showed that adding TiO2 nanoparticles influenced the amount of Staphylococcus aureus (p<0.05) and Candida albicans (p<0.05). A least significant difference test showed differences of effect in the amounts of Staphylococcus aureus when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base and there were differences in effect to amounts of Candida albicans when TiO2 concentrations of 2%, 3%, and 4% were added to heat-polymerized acrylic resin denture base.   Conclusion: Adding TiO2 nanoparticles to heat-polymerized acrylic resin denture base material affects the amounts of Staphylococcus aureus and Candida albicans.

Antibiofilm Effect of Clitoria ternatea Flower Juice on Porphyromonas gingivalis in vitro

Widyarman, Armelia Sari, Sumadi, Stephani, Agustin, Tri Putriany

Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

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Abstract

  Introduction: Clitoria ternatea flower contains flavonoid such as anthocyanin that gives the blue color to its flower and has antimicrobial activity.   Objectives: The aim of this study was to examine the effect of flower juice of Clitoria ternatea against Porphyromonas gingivalis biofilm viability in vitro.   Methods: This study was experimental laboratory research using biofilm assay method. P. gingivalis was cultured in BHI broth in 37°C for 24h under anaerobic condition. Fresh flowers of Clitoria ternatea were extracted using mortar and pestle and diluted into 6 different concentrations: 100%, 50%, 25%, 12.5%, 6.25%, and 3.125% with phosphate buffer saline (PBS). Chlorhexidine (0.2%) was used as positive control and biofilm without treatment as negative control. The flower juice was distributed into 96 well-plates that contained biofilm of P. gingivalis and incubated for 1h, 3h, 6h, and 24h in 37°C, anaerobic atmosphere. Biofilm was measured using crystal violet dye with microplate reader (490 nm). Data were statistically analysed using one-way ANOVA test and Post Hoc test with p<0.05 was set as significant different.   Result: Result showed that Clitoria ternatea flower juice significantly reduced the Porphyromonas gingivalis biofilm viability in all concentration and all incubation time. The most effective concentration to inhibit Porphyromonas gingivalis biofilm was 100% in 1h incubation time which biofilm was diminished (Optical Density=0.00). One way ANOVA test and Post Hoc test showed a significant biofilm reduction in all concentration and all incubation time after treatment with the flower juice compared to control (p<0.05).   Conclusion: Clitoria ternatea flower juice has antibiofilm effect against Porphyromonas gingivalis. This result showed this flower juice may be useful for combating periodontal pathogens. However, further studies using other bacteria are still needed to confirm this result.

The Comparison of Metronidazole, Clindamycin, and Amoxicillin Againts Streptococcus sanguinis

Lim, Kevin, Widyarman, Armelia Sari

Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

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Abstract

  Introduction: Viridans streptococci group such as Streptococcus sanguinis (S. sanguinis), an anaerobic Gram-positive bacteria is a well-known for its involvement in dry socket (alveolar osteitis)-associated infection. Systemic amoxicillin, clindamycin and metronidazole have all been shown to be effective to inhibit this bacterium. However, there has been a lack of studies identifying which are the most effective amongst these antibiotics toward Streptococcus sanguinis.   Objectives: The purpose of this study is to evaluate the effectiveness of metronidazole, clindamycin, and amoxicillin in inhibiting the growth of Streptococcus sanguinis in vitro.   Methods: This effectiveness was done by using agar well diffusion methods. S. sanguinis ATCC 10556 were cultured in Brain Heart Infusion (BHI) broth at 37°C under anaerobic condition. After 48h, bacterial cells were harvested and counted using microplate reader (490 nm) to achieve optical density of 0.25-0.30 (107 CFU/mL). Subsequently, 100 μL of bacterial suspension was cultured on BHI agar and each antibiotic suspension was added into each agar well, incubated for 72h at 37°C. The inhibition zone diameters were measured with electronic caliper. All experiments were done in triplicate, and repeated two times in separated occasions. The obtained data were statistically analyzed using one-way ANOVA test. A  p<0.05 was considered as significance.   Result: The results showed that there was a significant difference in the effectiveness, clindamycin and amoxicillin in inhibiting the growth of Streptococcus sanguinis (p<0.05), compared to metronidazole. The inhibition zone diameter with mean±SD (mm) are 13.50±2.0, 34.67±2.3 and 32.67±1.7 for metronidazole, clindamycin and amoxicillin, respectively.   Conclusion: Clindamycin and amoxicillin are more effective in inhibiting the growth of Streptococcus sanguinis compared to metronidazole in this study. However, future studies are needed to confirm this result in vivo.

Factors Influencing Requirements Implementation of Clinical Dental Student of Muhammadiyah University of Yogyakarta

Putranti, Wahyu Safitri Dhini, Dewanto, Iwan, Kurniasih, Indri, Astuti, Novitasari Ratna

Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

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Abstract

  Introduction: Institution of Dentist Education (IPDG) which requires professional students to meet a number of requirements tend to get constraints, such as delay in graduation due to students unable to meet all the requirements. This constraint relates to important factors in professional education, namely lecturer factor, patient factor, and student factor.   Objectives: This study aimed to understand the factors that influence the fulfillment of the requirements of clinical dental students of Muhammadiyah University of Yogyakarta (UMY).   Methods: This research was an observational research with cross sectional design. Research subjects amounted to 100, taken by purposive sampling technique. Research data consisted of primary and secondary data. Primary data were obtained from answers to questionnaires and secondary data were obtained from reports management information system in dental and mouth hospital of UMY. Research data were analyzed by Pearson correlation and multiple linear regression.   Results: There was a difference between the questionnaire answers and the reports of management information system regarding the fulfillment of the requirements. Pearson correlation analysis results obtained significance value of the three factors of 0.000 (p<0.05). The result of multiple linear regression analysis showed that lecturer factor had significance value 0.003 (p<0.05), patient factor had significance value equal to 0.013 (p<0.05), and student factor had significance value equal to 0.001 (p<0.05). Simultaneously factor of lecturer, patient factor, and student factor significantly influence to fulfill requirement of clinical dental student with F count equal to 24.836 and significance number 0.000. The contribution of these three factors had an influence of 41.9%, the remaining 58.1% was determined by other variables that were not in this study.   Conclusion: Lecturer factor, patient factor, and student factor have relevance and influence the fulfillment of the requirements of clinical dental students of UMY.

Barriers to the Implementation of Dental Insurance in Indonesia as Perceived by Primary Dentists

Dewanto, Iwan, Koontongkaew, Sitichai, Widyastuti, Niken

Journal of Indonesian Dental Association Vol 1 No 1 (2018): October
Publisher : Indonesian Dental Association

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Abstract

  Introduction: In 2014, the government of Indonesia launched the National Health Insurance (NHI) system. The system faced challenges in terms of its implementation because it changed the funding scheme within the pre-established health services structure. Under the new NHI system, the funding scheme for primary care providers is a capitation system; in the field of dental care, this means primary dentists received payment based on the amount number of participants enrolled within their geographical area.   Objectives: The aim of this study is to describe primary dentists’ perceived constraints toward the implementation of the NHI system and their level of knowledge about managed care in Yogyakarta, Indonesia.   Methods: To determine dentists’ perceptions about the implementation of NHI and their knowledge about managed care, we surveyed dentists in a descriptive study. Two types of questionnaire were administered to 91 dentists who work at community health centers (CHCs) in Yogyakarta, using the total population sampling technique.   Result: Through the surveys, we determined that 78 dentists (71%) perceived constraints related to capitation, 65 dentists (72%) perceived constraints related to benefits packages, 59 dentists (65%) perceived constraints related to workload, and 23 dentists (25%) perceived constraints related to dental health facilities. In terms of their knowledge of managed care, 46 respondents (50.5%) demonstrated a good knowledge of managed care, 43 respondents (47.3%) had moderate knowledge, and 2 respondents (2.2%) had poor knowledge.   Conclusion: The constraints to managed care, as perceived by primary dentists, should be taken into account when implementing the NHI system in Yogyakarta, as these perceptions could affect the system’s success.