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JURNAL SELULOSA
Jurnal Selulosa (JSel) is a journal that provides scientific information resources aimed at researchers and engineers in academia, research institutions, government agencies, and industries. Jurnal Selulosa publishes original research papers, review articles and case studies focused on cellulose, cellulose derivatives, pulp technology, paper technology, environment, biorefinery and other related topics. Formerly known as Berita Selulosa, and the first publication was in 1965. Since 2011, the journal renamed to Jurnal Selulosa.
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164
Articles
Produksi β-Glukosidase Aspergillus niger BIO 2173 dengan Fermentasi Padat Menggunakan Substrat Dedak

Sugiwati, Sri, Suhartono, Maggy Thenawidjaja, Hanafi, Muhammad, Lioe, Hanifah Nuryani

JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

Production of β-Glucosidase Aspergillus niger BIO 2173 on Solid State Fermentation Using Rice Bran as SubstrateAbstractβ-Glucosidase (EC 3.2.1.21) is a part of the cellulase enzyme complex which acts synergistically with exoglucanase and endoglucanase to hydrolyze cellulose into glucose. The purpose of this study was to obtain the maximum fermentation conditions for production of b-glucosidase Aspergillus niger BIO 2173 with solid state fermentation using rice bran as fermentation substrate. The factors that affect the production of b-glucosidase which consist of initial pH of the fermentation medium, incubation period, ratio of water content to fermentation substrate, incubation temperature and addition of the Mandel’s mineral salts solution were examined in the study. The results showed that maximum fermentation conditions for β-glucosidase production were at initial of fermentation pH of 2,0, incubation period of 7 days, ratio of water content to substrate of 1:1, and incubation temperature of 32oC. Addition of Mandel’s mineral salts solution to the fermentation substrate at maximum fermentation conditions increased the activity and specific activity of β-glucosidase crude extract up to 5,24 ± 0,57 U/mL and 2,46 ± 0,04 U/mg, respectively.Abstrakβ-Glukosidase (EC 3.2.1.21) merupakan bagian dari enzim multi kompleks selulase, yang bekerja secara sinergis dengan eksoglukanase dan endoglukanase menghidrolisis selulosa menjadi glukosa. Tujuan dari penelitian ini adalah mendapatkan kondisi fermentasi maksimum untuk produksi β-glukosidaseAspergillus niger BIO 2173 dengan fermentasi media padat menggunakan substrat dedak. Pengujian dilakukan terhadap faktor-faktor yang mempengaruhi produksi b-glukosidase, yaitu pH awal medium fermentasi, waktu inkubasi, perbandingan kandungan air terhadap substrat medium fermentasi, suhu inkubasi dan penambahan larutan garam mineral Mandels. Hasil penelitian menunjukkan bahwa kondisi fermentasi maksimum untuk produksi b-glukosidase adalah pada pH awal medium fermentasi 2,0; waktu inkubasi 7 hari, perbandingan kandungan air terhadap substrat medium fermentasi 1:1, dan suhu inkubasi 32oC. Penambahan larutan garam mineral Mandels ke dalam substrat fermentasi pada kondisi fermentasi maksimum menyebabkan peningkatan aktivitas dan aktivitas spesifk ekstrak kasar b-glukosidase masing-masing sebesar 5,24 ± 0,57 U/mL dan 2,46 ± 0,04 U/mg protein. Kata kunci: β-glukosidase, Aspergillus niger, dedak padi, fermentasi padat, ekstrak kasar

Potensi Penggunaan Dua Spesies Agave untuk Pembuatan Pulp dan Kertas

Jamil, Arini Hidayati, Tjahjono, Heronimus Judi, Parnidi, Parnidi, Marjani, Marjani

JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

Potential of Two Agave Species for Pulp and Paper MakingAbstractBoth of Agave sisalana and A. cantala are the most favorite agave species for fiber producer plant in Indonesia. Nevertheless, research study for using fiber of A. sisalana and A. cantala for pulp and paper purpose is hard to find. This study aims to observe the characteristics of A. sisalana and A. cantala fibers as a raw material of pulp including fiber morphology, chemical components, pulp characteristics, optical and physical properties of the handsheets. The observation of all characters was based on Indonesian National Standard (SNI). The pulping method used both of soda process with 18% active alkali and kraft process with 18% active alkali and 25% sulfidity. The result of the study for the handsheets from A. sisalana and A. cantala fibers shows that the brightness, dirt, and tear index were fulfilled to SNI standard for NBKP while the tensile index and burst index just fulfilled to SNI standard for LBKP. The handsheet of A. sisalana fiber has a higher tear index, tensile index, and burst index than the A. cantala ones. Soda pulping of both agave species resulting higher pulp yield, cellulose polymerization degrees and physical properties than kraft pulping.Keywords : Agave sisalana, A. cantala, characteristics of the fiber, pulp AbstrakAgave sisalana dan A. cantala merupakan dua spesies agave yang paling banyak dibudidayakan di Indonesia sebagai tanaman penghasil serat alam. Namun penelitian mengenai fungsi serat A. sisalana dan A. cantala sebagai bahan baku pulp dan kertas masih sulit ditemukan. Tujuan dari penelitian ini adalah mempelajari karakteristik serat A. sisalana dan A. cantala sebagai bahan baku pulp yang meliputi morfologi serat, komposisi komponen kimia, karakeristik pulp, sifat optik dan fisik lembaran pulp yang dihasilkan. Pengamatan seluruh karakter dilakukan berdasarkan Standar Nasional Indonesia. Pemasakan menggunakan metode soda dengan alkali aktif 18% dan kraft dengan alkali aktif 18% dan sulfiditas 25%. Hasil pengamatan pada lembaran pulp yang dihasilkan dari serat A. sisalana dan A. cantala menunjukkan bahwa derajat cerah, noda, dan kekuatan sobek memenuhi Standar Nasional Indonesia (SNI) Pulp Kraft Putih Kayujarum (NBKP) sementara kekuatan tarik dan kekuatan retak memenuhi SNI Pulp Krat Putih Kayudaun (LBKP). Lembaran pulp A. sisalana memiliki kekuatan sobek, tarik, dan retak lebih tinggi dibanding lembaran pulp A. cantala. Metode pemasakan soda pada serat kedua spesies agave tersebut menghasilkan rendemen pulp, derajat polimerisasi selulosa, dan kekuatan fisik lebih baik dibanding metode kraft.Kata kunci : Agave sisalana, A. cantala, karakteristik serat, pulp 

Pembuatan dan Karakterisasi Dissolving Pulp Serat Panjang dari Bambu Duri (Bambusa blumeana)

Purwita, Chandra Apriana, Sugesty, Susi

JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

Preparation and Characterization of Long Fiber Dissolving Pulp from Spiny Bamboo (Bambusa blumeana)The need for long fiber dissolving pulp in Indonesia can only be met from imports. Bamboo is a nonwood plant and known as source of long fiber. This research aims to study the potential of spiny bamboo to be used as raw material for producing long fiber dissolving pulp. This research was conducted using two different types of raw materials preparation to produce bamboo chip and decorticated bamboo. The pulping process is carried out by pre-hydrolysis kraft and bleaching performed with two different bleaching sequences, i.e Do ED1 D2 and Do EpD1 D2 . Based on the experimental results, spiny bamboo has good potential to be used as raw material for dissolving pulp. Spiny bamboo belongs to long fibers with an average fiber length of 2.46 mm. The dissolving quality depends on the preparation of the raw material and the bleaching sequence. The yield of spiny bamboo dissolving pulp ranged from 37.97 - 40.76% with alpha cellulose content of 94.88 - 98.67%, and viscosity of 16.43 - 25.75 cP. Decorticated bamboo with bleaching sequence of Do EpD1 D2 produced the highest quality of dissolving pulp with the highest brightness and alpha cellulose were 89.61% ISO and 98.67%, respectively.AbstrakKebutuhan dissolving pulp serat panjang di Indonesia hanya dapat dipenuhi melalui impor. Bambu adalah tanaman nonkayu dan dikenal sebagai sumber serat panjang. Penelitian ini bertujuan untuk mempelajari potensi bambu duri untuk dijadikan bahan baku pembuatan dissolving pulp serat panjang. Penelitian ini dilakukan dengan dua jenis persiapan bahan baku yang berbeda untuk menghasilkan serpih bambu dan bambu dekortikasi. Proses pemasakan dilakukan dengan proses pra-hidrolisis kraft dan pemutihan dilakukan dengan dua urutan pemutihan yang berbeda, yaitu  DoED1D2 dan DoEpD1D2. Berdasarkan hasil penelitian, bambu duri memiliki potensi yang baik untuk digunakan sebagai bahan baku pembuatan dissolving pulp. Bambu duri tergolong serat panjang dengan panjang serat rata-rata 2,46 mm. Kualitas dissolving pup yang dihasilkan tergantung dari persiapan bahan baku dan urutan pemutihan. Rendemen dissolving pulp bambu duri berkisar 37,97 - 40,76%, dengan kandungan selulosa alfa 94,88 - 98,67%, dan viskositas 16,43 - 25,75 cP. Bambu dekortikasi dengan urutan pemutihan DoEpD1D2 menghasilkan dissolving pulp paling unggul dengan derajat cerah dan selulosa alfa tertinggi berturut-turut 89,61 %ISO dan 98,67%.Kata kunci: dissolving pulp, bambu duri (Bambusa blumeana), serpih bambu, bambu dekortikasi, prahidrolisis kraft 

Serat Bambu Petung (Dendrocalamus asper) Teralkalisasi sebagai Penguat Komposit Polimer

Refiadi, Gunawan, Bayu, Novan, Judawisastra, Hermawan, Mardiyati, Mardiyati

JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

The alkalized of petung bamboo fiber (Dendrocalamus asper) as reinforcement of polymer compositeAbstractPetung bamboo (Dendrocalamus asper) has good fiber characteristic and it has potential to substitute glass fiber as reinforcement in polymeric composite. However, like other natural based materials, bamboo fiber has a high variability in mechanical properties and inherently hygroscopic characteristic. Therefore it is required to optimize the petung bamboo qualities. The fiber optimization was carried out by alkalization process using NaOH solution with varying concentration of 0, 3, 5, and 10% v/v. Bamboo fiber processed in chemomechanical was used in this investigation. Characterization of density, mechanical and chemicals was conducted by pycnometry method, tensile strength testing, Fourier Transform Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The data variability was analyzed by Weibull distribution. Result indicates that the optimum condition of alkalization process of bamboo fiber using NaOH solution of 5% v/v which has fiber diameter of 175.098 ± 58.017 mm, tensile strength of 384 MPa, and characteristics strength of 390.5 MPa.Keywords: petung bamboo; alkalization; characteristics AbstractBambu petung memiliki karakteristik serat yang baik sehingga berpotensi sebagai pengganti serat gelas untuk penguat komposit polimer. Namun, seperti umumnya serat alam, serat bambu memiliki variabilitas sifat mekanis tinggi dan sifat higroskopik yang inheren. Oleh karena itu diperlukan upaya optimalisasi kinerja serat bambu petung. Optimalisasi kinerja serat dilakukan melalui proses alkalisasi dengan variasi larutan NaOH pada konsentrasi 0, 3, 5, dan 10% v/v. Serat bambu hasil dari proses kimia-mekanik digunakan dalam penelitian ini. Karakterisasi densitas, sifat mekanik, dan sifat kimia serat masing-masing dilakukan dengan metode piknometri, uji tarik, Fourier Transform Spectroscopy (FTIR), Scanning Electron Microscopy (SEM). Analisis variabilitas data menggunakan distribusi Weibull. Hasil menunjukkan bahwa kondisi optimum alkalisasi serat bambu petung yaitu menggunakan larutan NaOH 5% v/v yang menghasilkan serat berdiameter 175,098 ± 58,017 mm, kekuatan Tarik 384 MPa, dan kekuatan karakteristik 390,5 MPa.Kata kunci: bambu petung; alkalisasi; karakteristik 

Pemanfaatan Lumpur Primer Industri Kertas sebagai Absorben

Wardhana, Krisna Adhitya, Saepulloh, Saepulloh, Biantoro, Reynaldo

JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

The Utilization of Primary Sludge from Paper Mills as AbsorbentAbstractThe primary sludge from paper mill contains cellulose fiber that has high absorption capacity and potential to be used as an absorbent for hydrophobic compounds. The utilization of primary sludge from paper mill using raw material of waste paper and virgin pulp to be used as an absorbent has been conducted. The stages process of absorbent fabrication was consisted of three stages such as drying, mechanical and chemical processes. Mechanical process conducted were crushing and grinding methods to increase surface area and pore volume of sludge. Meanwhile silanization process as chemical treatment using solution of γ-metacryloxypropyl trimethoxysilane in ethanol to improve the hydrophobic properties of primary sludge was also done. Absorbent products were tested for the maximum sorbency, the sorbent performance, absorption of hydrophobic liquid, absorption of hydrophobic dust, and contact angle test to conduct their performance. The absorbency of absorbent product which is covered by cushion material was also tested. Result indicated that the absorption performance of some absorbent product is higher than commercial absorbent on hydrophobic liquid and hydrophobic dust absorption test. But it was lower than commercial absorbent on maximum sorbency and the sorbent performance test. The use of cushion material as a wrapper could make it easy in the collection and could increase the absorbency.Keywords: primary sludge; mechanical; silanization; absorbent; hydrophobic AbstrakLumpur primer dari industri kertas mengandung serat selulosa yang memiliki daya serap tinggi dan memiliki potensi dimanfaatkan sebagai absorben senyawa hidrofobik. Pemanfaatan lumpur primer industri kertas berbahan baku kertas bekas dan virgin pulp sebagai absorben telah dilakukan. Tahapan proses pembuatan absorben dari lumpur primer meliputi proses pengeringan, proses mekanis, dan proses kimia. Proses mekanis yang dilakukan adalah crushing dan grinding untuk meningkatkan luas permukaan dan volume pori-pori lumpur dilanjutkan dengan proses silanisasi (silanization) sebagai perlakuan kimia menggunakan larutan γ-metacryloxypropyl trimethoxysilane dalam etanol untuk meningkatkan sifat hidrofobik lumpur. Produk absorben diuji daya serap maksimum (maximum sorbency), kinerja absorben, penyerapan cairan hidrofobik, penyerapan debu hidrofobik, dan pengujian sudut kontak. Daya serap produk absorben yang ditutup bahan bahan cushion juga diuji. Hasil menunjukkan kemampuan absorbsi cairan hidrofobik dan debu hidrofobik dari beberapa perlakuan produk absorben lebih tinggi dari absorben komersial, sedangkan hasil maximum sorbency dan uji kinerja absorben menunjukkan hasil lebih rendah dari absorben komersial. Penggunaan bahan cushion sebagai pembungkus dapat memudahkan dalam pengumpulan dan dapat meningkatkan penyerapan.Kata kunci: lumpur primer; mekanis; silanisasi; absorben; hidrofobik 

HUBUNGAN SPECIFIC GRAVITY KAYUDAUN TERHADAP SERAT DAN KUALITAS PULP (THE RELATIONSHIP OF HARDWOOD SPECIFIC GRAVITY TO FIBER AND PULP QUALITY)

Haroen, Wawan Kartiwa

JURNAL SELULOSA Vol 7, No 02 (2017): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

The demand for hardwood pulp increased due to the limited hardwood in several countries and its advantage as raw material for paper or paper boards. The hardwood has a specific grafity ranging from low to high that causes the quality of pulp produced not uniform. The observation of specific gravity, fiber morphology, and chemical content of hardwood; and the quality of hardwood pulp have been done. FAO data were used to evaluate the fiber length, fiber wall, lignin, extractives, Kappa Number, and the quality of sulphate pulp. The sulfidity, active alkali, solid to liquor ratio, temperature, and cooking time of pulping process were 25%, 17%, 1 :4, 170°C, and 3.5 hours, respectively. The specific gravity of hardwood in the range of 0.30 to 0.99 was grouped into seven classes and analyzed by statistical regression. The specific gravity of the hardwood was associated withfiber morphology, chemical content, pulping, and pulp quality. The results showed that tha specific gravity of wood affects certain parameters with regression equation model that can be used for the selection ofwood raw materials for sulphate pulping. There is a tendency that the specific gravity can influence the hardwood fiber wall thickness, lignin content, pulp yield, Kappa Number, and the physical properties of pulp for tear and tensile srength. ABSTRAKPermintaan terhadap pulp kayudaun terjadi peningkatan akibat terbatasnya kayudaun di beberapa negara dan keunggulannya sebagai bahan baku kertas atau karton. Kayudaun memiliki specific gravity rendah sampai tinggi, menyebabkan kualitas pulp yang dihasilkan tidak seragam. Pengamatan specific gravity, morfologi serat,dan kandungan kimia kayudaun, serta kualitas pulp telah dilakukan. Evaluasi dilakukan menggunakan data FAO terhadap panjang serat, dinding serat, kadar lignin, ekstraktif, bilangan Kappa, dan kualitas pulp sulfat.Kondisi pemasakan sulfiditas 25%, alkali aktif 17% , ratio 1:4, suhu 170 o C, dan waktu 3,5 jam. Specific gravity kayudaun 0,30-0,99 dikelompokkan kedalam tujuh kelas dan dianalisis dengan statistik regresi. Specific gravity kayu sebagai data tetap dihubungkan dengan morfologi serat, kimia kayu, pulping, dan kualitas pulp. Hasil menunjukkan bahwa specific gravity mempengaruhi parameter tertentu dengan model persamaan regresi yang dapat digunakan untuk seleksi bahan baku hardwood untuk pembuatan pulp sulfat. Terdapat kecenderungan bahwa specific gravity kayudaun dapat mempengaruhi ketebalan dinding serat, kadar lignin, rendemen pulp, bilangan Kappa, sifat fisik pulp untuk sobek, dan tarik.

POTENSI KERTAS SEBAGAI BAHAN BAKU PAPER ROPES (POTENTIAL OF PAPER AS THE RAW MATERIAL FOR PAPER ROPES)

Romas, Mungki Septian, Pramuaji, Ikhwan, Indriati, Lies, Wirawan, Sonny Kurnia

JURNAL SELULOSA Vol 7, No 02 (2017): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

One of the raw materials furniture common in Indonesia is rattan. Because of the availability of local rattan is very limited so that it is necessary to find alternative raw materials that can be used to substitute the rattan. Paper ropes made from spinning paper, is one of alternative that can be utilized. Paper ropes making from various spinning papers have been studied. Some types of paper were used in this experiment. The spinning papers were characterized and treated to modify its surface properties especially to increase their water resistance. Paper ropes making were done by using the twisting machine and then the physical properties of paper ropes resulted were tested. The results showed that paper surface treatment increased tensile strength, and reduced water absorption and porosity of treated papers. The use of water barrier chemicals and adhesive are effective in increasing water resistance of paper surface. Decreased water absorption and porosity are  20% - 43% and 30% - 98%, respectively. Based on this result, paper ropes is potential to become an alternative raw material for furniture.  ABSTRAKSalah satu bahan baku furnitur umum di Indonesia adalah rotan. Karena ketersediaan rotan lokal sangat terbatas sehingga diperlukan untuk mencari bahan baku alternatif yang dapat digunakan untuk menggantikan rotan. Paper ropes yang terbuat dari spinning paper, merupakan salah satu alternatif yang dapat dimanfaatkan. Pembuatan paper ropes dari berbagai jenis spinning paper telah dipelajari pada penelitian ini. Beberapa jenis kertas digunakan dalam percobaan ini. Spinning paper dikarakterisasi dan diperlakukan khusus untuk memodifikasi sifat permukaannya terutama untuk meningkatkan ketahanan airnya. Pembuatan paper ropes dilakukan dengan menggunakan mesin pemilin dan pengujian sifat fisik paper ropes telah dilakukan. Hasil penelitian menunjukkan bahwa perlakuan khusus permukaan kertas meningkatkan kekuatan tarik, dan mengurangi penyerapan air dan porositas. Penggunaan bahan kimia water barrier dan adhesif cukup efektif dalam meningkatkan ketahanan air permukaan kertas.  Penurunan penyerapan air dan porositas masing-masing sebesar 20% - 43% dan 30% - 98%. Berdasarkan hasil ini, paper ropes memiliki potensi sebagai bahan bahan baku alternatif untuk furnitur.

SAKARIFIKASI LUMPUR PRIMER INDUSTRI KERTAS SECARA FED BATCH MENJADI GLUKOSA UNTUK PEMBUATAN BIOETANOL (FED BATCH SACCHARIFICATION OF PRIMARY PAPER MILL SLUDGE TO PRODUCE FERMENTABLE SUGAR)

Aini, Mukharomah Nur, S. Soetopo, Rina, Septiningrum, Krisna, Saepulloh, Saepulloh, Asthary, Prima Besty

JURNAL SELULOSA Vol 7, No 02 (2017): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

Research of fed batch saccharification using paper mill primary sludge from Waste Water Treatment Plant (WWTP) has been conducted. The research was conducted in several stages i.e. sludge characterization, determining optimum dosage of cellulase and glucosidase through batch saccharification, and continued with fed batch saccharification. The characterization was performed on 3 primary sludge type from 3 paper mill, which are print paper mill with virgin pulp raw material, paper tissue mill with virgin pulp raw material and core board mill with waste paper raw material. The characterization results showed that the sludge derived from the tissue paper mill with virgin pulp raw material has the highest content of cellulose which is 47.06%, this sludge was used as further research material. The initial trial of batch saccharification at 6% w/w and temperature of 60oC gave optimum dosage of cellulase 9 FPU/g cellulose and β-glucosidase 80 IU/g cellulose with reducing sugar 20.01 g/L and a saccharification efficiency of 63.84%. Fed batch saccharification was performed on tissue paper mill sludge at cellulase and glucosidase enzyme doses respectively 9 FPU/g cellulose and 80 IU/g, temperature 60°C and initial pH of 4 with two variables, which are cumulative solid content (10%, 14%, and 18% w/v) and feed enzyme system (at the beginning and in step). The best results were obtained at fed batch sakarifikasi with gradual enzyme feeding at 14% solids content resulting in reducing sugar 43.899 g/L and efficiency 60.03%. 

BIODEINKING SORTED WHITE LEDGER (SWL) MENGGUNAKAN SELULASE (BIODEINKING SORTED WHITE LEDGER (SWL) BY CELLULASE)

Purwita, Chandra Apriana, Risdianto, Hendro Kurnia

JURNAL SELULOSA Vol 7, No 02 (2017): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

Biodeinking is a process of separating ink on the fiber surface by enzymes. This research was conducted to study and optimize biodeinking condition of sorted white ledger (SWL) by cellulase. Commercial cellulase (Sigma Aldrich, Singapore) at a dose of 0.25; 0.5; 0.75; 1; 2 U/g dry weight pulp was applied to the deinking of SWL and compared with blank. The deinked pulp was then analyzed for the fiber morphology and made laboratory hand sheets for the characterization of physical and optical properties. Based on the experiment, at the optimum dose of cellulase 0.75 IU/g dry weight pulp was obtained a maximum increasement of ISO brightness by 4.7 points and a decreasement of ERIC by 34.45%. The use of celulase on the biodeinking was not affecting the fibers length and diameter so the tensile and tear index of deinked pulp unchanged. Loss of fines by enzymatic degradation causes the tear index decreased by 8.70 % and porosity increased. The increasement of coarseness resulted in the decreasement of pulp opacity.ABSTRAKBiodeinking merupakan proses pemisahan tinta pada permukaan serat menggunakan enzim. Penelitian ini dilakukan untuk mempelajari dan memperoleh kondisi optimum biodeinking sorted white ledger (SWL) menggunakan selulase. Selulase komersial (Sigma Aldrich, Singapura) dengan dosis 0,25; 0,5; 0,75; 1; 2 U/g kering pulp diaplikasikan pada deinking SWL dan dibandingkan terhadap blanko. Pulp deinked yang diperoleh kemudian dilakukan analisis morfologi serat dan dibuat lembaran tangan laboratorium untuk karakterisasi sifat fisik dan optik. Berdasarkan percobaan, pada dosis optimum selulase 0,75 IU/g kering pulp diperoleh kenaikan derajat cerah ISO maksimum sebesar 4,7 poin dan penurunan ERIC 34,45%. Analisis morfologi serat menunjukkan penggunaan selulase pada proses biodeinking tidak berpengaruh terhadap panjang dan diameter serat sehingga indeks tarik dan retak pulp deinked tidak berubah. Kehilangan fines karena degradasi enzimatik menyebabkan indeks sobek turun sebesar 8,70% dan porositas naik. Naiknya nilai coarseness menyebabkan meningkatnya opasitas pulp deinked.

PENGARUH KONDISI KULTUR PADA AKTIVITAS SELULASE ISOLAT Pycnoporus sp. DAN Phlebiopsis sp. (EFFECTS OF CULTURE CONDITIONS ON CELLULASE ACTIVITIES PRODUCED BY Pycnoporus sp. AND Phlebiopsis sp.)

Agustini, Luciasih, Irianto, Ragil S.B, Turjaman, Maman, Faulina, Sarah Asih, Ariantari, Resti, Stephandra, Sira, Yuniar, Herni, Aryanto, Aryanto, Najmulah, Najmulah, Yani, Ahmad

JURNAL SELULOSA Vol 7, No 02 (2017): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

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Abstract

The effects of media, pH and temperature on cellulase-complex enzyme produced by Pycnoporus sp. FORDACC-03452 and Phlebiopsis sp. FORDACC-02482 cultivated in rice bran and corn cobs media under solid state fermentation with pH 4–7 and temperature 30°C–45°C were investigated. Rice bran media showed a propensity to induce endo-β,1,4-glucanase and cellobiohydrolase productions, while corn cobs media induce β-glucosidase production. However, the mixture of rice bran and corn cobs did not result in better cellulase complex enzyme activities. Cellulase-complex produced by Pycnoporussp. showed superior activities compared to those produced by Phlebiopsissp. Crude enzyme of Pycnoporus sp. showed optimum specifc-activities of endo-β-1,4-glucanase at pH 6, temperature 35°C (0.403 ± 0.010 IU/mg), cellobiohydrolase at pH 6, temperature 40°C (0.540 ± 0.020 IU/mg) and β-glucosidase at pH 4, temperature 30 °C (0.022 ± 0.001 IU/mg). While Phlebiopsis sp. showed optimum specifc-activities of endo-β-1,4-glucanase at pH 6, temperature 35°C (0.202 ± 0.005 IU/mg), cellobiohydrolase at pH 4, temperature 45°C (0.180 ± 0.002 IU/mg) and β-glucosidase at pH 6, temperature 45°C (0.007 ± 0.001 IU/mg). Due to low β-glucosidase activities, the cellulase-complex generated from this study were not able to completely hydrolyse lignocellulosic waste and yielded unsufficient sugars content. Further investigation to optimize cellulase-complex production from these fungal isolates is still required.ABSTRAKPenelitian pengaruh media kultivasi, pH dan suhu inkubasi terhadap produksi enzim selulase-kompleks dari Pycnoporus sp. FORDACC-03452 dan Phlebiopsis sp. FORDACC-02482 yang ditumbuhkan di media dedak padi dan tongkol jagung dengan metode kultur padat pada variasi pH 4–7 dan suhu 30°C–45°C, telah dilakukan. Hasil memperlihatkan bahwa media dedak padi cenderung menginduksi produksi endo-β-1,4-glukanase dan selobiohidrolase, sedangkan media tongkol jagung menginduksi produksi β-glukosidase. Namun, campuran kedua substrat tersebut tidak menghasilkan aktivitas selulase yang lebih baik. Selulase-kompleks yang dihasilkan Pycnoporus sp. menunjukkan aktivitas lebih baik dibandingkan dengan yang diproduksi Phlebiopsis sp. Filtrat kasar Pycnoporus sp. menunjukkan aktivitas-spesifk endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,403 ± 0,010 IU/mg); selobiohidrolase pada pH 6, suhu 40°C (0,540 ± 0,020 IU/mg); dan β -glukosidase pada pH 4, suhu 30°C (0,022±0,001 IU/mg). Sementara, Phlebiopsis sp. menunjukkan aktivitas-spesifk endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,202 ± 0,005 IU/mg); selobiohidrolase pada pH 4, suhu 45°C (0,180 ± 0,002 IU/mg); dan β-glukosidase pada pH 6, suhu 45°C (0,007 ± 0,001 IU/mg). Rendahnya aktivitas β-glukosidase menyebabkan selulase-kompleks dari penelitian ini belum dapat menghidrolisis limbah lignoselulosa dengan sempurna dan kadar glukosa yang diperoleh masih rendah. Oleh karena itu, optimasi produksi selulasekompleks dari Pycnoporus sp. dan Phlebiopsis sp. masih perlu diteliti lebih lanjut.