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INDONESIA
Indonesian Aquaculture Journal
ISSN : 02150883     EISSN : 25026577     DOI : -
Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.
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Articles 9 Documents
Search results for , issue " Vol 8, No 2 (2013): (December 2013)" : 9 Documents clear
STUDY OF MICROFLORA PATHOGENIC TO FISH IN BIOREMEDIATED SEWAGE EFFLUENT Khurshid, Almas; Ahmad, I; Kausar, Rehana
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (869.175 KB) | DOI: 10.15578/iaj.8.2.2013.135-161

Abstract

To assess the suitability of bioremediated sewage effluent from Shehzad Town, Islamabad for fish cultivation, it was tested for the presence of microflora pathogenic to fish and also for its water quality, at National Agriculture Research Centre (NARC), Islamabad. There was no significant difference found in the microflora of fresh and bioremediated sewage effluent and no evidence of disease causing species was observed during the study. A total of 15 fungal isolates and 7 bacterial isolates were obtained from bioremediated sewage effluent. Identified isolated microflora was found to be non-pathogenic to fish. Most prominent fungal species were that of Aspergillus, Alternaria, and Penicillium, whereas Pseudomonas, Proteus, and Elizabeth were prominent bacterial species in both fresh and bioremediated sewage effluent.
EFFECTIVITY OF INACTIVE GSDIV (GROUPER SLEEPY DISEASE IRIDOVIRUS) VACCINE IN GROUPER FISH (Cromileptes altivelis and Epinephelus fuscoguttatus) AGAINST GSDIV INFECTION Mahardika, Ketut; Mastuti, Indah; Haryanti, Haryanti
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1197.996 KB) | DOI: 10.15578/iaj.8.2.2013.143-151

Abstract

Grouper sleepy disease iridovirus (GSDIV) has been known as viral agent causing mass mortality and significant economic losses in Indonesian aquaculture industry. The aim of this research was to know the effectivity of formalin-inactivated GSDIV vaccine to prevent GSDIV infection in humpback grouper (Cromileptes altivelis) and tiger grouper (Epinephelus fuscoguttatus). The vaccine was derived from GF cells infected-GSDIV which activated using formalin. Used vaccine was contained titer virus of 102,80-105,80 TCID50/ mL. Result of vaccination test indicated that a vaccinated grouper fish with formalininactive GSDIV vaccine should decreased mortality rate of 5%–85.4% in humpback grouper and tiger grouper that infected with GSDIV. Histopathologically, infected fish from vaccinated and control groups showed formation of enlarged cells as well as necrotized cells especially in spleen and kidney tissues. On the other hand, surviving fish from both vaccinated and control groups did not contained formation of enlarged cells in spleen, kidney, liver, and stomach.
EXPRESSION OF GROWTH HORMONE (PhGH) GENE AND ANALYSIS OF INSULINE-LIKE GROWTH FACTOR I (IGF-I) PRODUCTION IN AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC F-1 Marnis, Huria; Iswanto, Bambang; Suprapto, Rommy; Imron, Imron
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.581 KB) | DOI: 10.15578/iaj.8.2.2013.113-119

Abstract

We have previously produced F-1 transgenic of African catfish from crosses between founder transgenic female and non transgenic male. The aim of this study was to evaluate distribution and expression PhGH growth hormone gene transgenic African catfish organs and to measure the concentration of IGF-I in plasma. Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, gill, and eye. Transgene expression levels were analyzed using the method of quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR). Plasma samples were analyzed for Insuline-like Growth Factor (IGF-I) using Enzyme Linked Immunosorbent Assay (ELISA) method. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish (F-1). Liver exhibited the highest level of PhGH mRNA (23 x 106 copies). The plasma IGF-I levels in transgenic individuals were not significant than non transgenic. The higher level of exogenous PhGH gene expression may not represent the production of IGF-1.
FEED MANAGEMENT AND MOLASSES APPLICATION ON THE INTENSIVE MILKFISH CULTURE (Chanos chanos Forsk.) IN PONDS Pantjara, Brata; Hendrajat, Erfan Andi; Kristanto, Anang Hari
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (862.348 KB) | DOI: 10.15578/iaj.8.2.2013.153-161

Abstract

The problems faced in the intensive milkfish culture were the high and expensive feeds requirement. To overcome the problem, it needs the optimization of feed management with application of molasses for producing of bioflocs in pond. Bioflocs were a biomass set of heterotrophic bacteria, protozoa, plankton, and organic particle. The purpose of this research was to know the influence of feed management and application of molasses on the intensive culture of milkfish in ponds. The study was conducted in brackishwater pond with 2,500 m2 size; conducting at experimental pond installation, Research and Development Institute for Coastal Aquaculture, Maros. The treatments of this research were without reduction commercial feeds as control (A); 10% reduction of commercial feed and molasses application (B); and 20% reduction of commercial feed and molasses application (C). Reduction of commercial feed was as many as 10% and 20% of the feed given doses of each application. The results of this research showed that intensive milkfish culture with biofloc was not significantly increase of the growth and production of milkfish. The highest of milkfish production was obtained in A treatment with 7,260 kg/ha; followed by B (6,339 kg/ha) and the lowest was in C (5,780 kg/ha).
STUDY PROPAGATION OF GSDIV (GROUPER SLEEPY DISEASE IRIDOVIRUS) IN CULTURED GF (GRUNT FIN) CELL Mahardika, Ketut; Mastuti, Indah
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (861.531 KB) | DOI: 10.15578/iaj.8.2.2013.121-126

Abstract

Grouper Sleepy Disease Iridovirus (GSDIV, genus Megalocityvirus) remain unsolved in Indonesian aquaculture. A GSDIV propagating study had been conducted to elaborate the capability of GSDIV replication in GF cell line. GSDIV isolate derived from spleen of diseased grouper fish were infected to GF cells line. GSDIV-infected GF cells were characterized by enlargement cells as CPE (cytophatic effect) which floated in cultured-medium. CPE-GSDIV was positively confirmed by PCR test. GSDIV-viral concentrations were ranging between 102.80-105.80 TCID50/mL. The newly passage GF cells were more sensitive to GSDIV which was shown by the abundance of CPE. GSDIV derived from GF cells were caused 10%-100% mortality of humpback grouper, Cromileptes altivelis.
EFFECT OF TEMPERATURE AND LIGHT ON THE PHOTOSYNTHESIS AS MEASURED BY CHLOROPHYLL FLUORESCENCE OF CULTURED Kappaphycus sp. (SAKOL STRAIN) FROM INDONESIA Lidemen, Lidemen; Laining, Asda; Nishihara, George N; Terada, Ryuta
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (986.166 KB) | DOI: 10.15578/iaj.8.2.2013.163-171

Abstract

Photosynthetic performance of carrageenophyte (Solieriaceae; Rhodophyta) cultured in Indonesia, Kappaphycus sp. (Sakol strain), was investigated at various temperature and light conditions related to their cultivation performance. A “pulse-amplitude modulatedchlorophyll fluorometer” (Diving-PAM) was used to generate a rapid light curves (RLCs) to provide estimates relative electron transport rates (rETR) for over 10 temperatures (i.e., 16oC to 34oC) and at nine photosynthetic active radiation (PAR) levels, which ranged from 0 to 1,000 mol photons m-2 s-1. The initial slope (α), photo-inhibition coefficient (β), and the coefficient of maximum photosynthesis assuming no photo-inhibition (γ) is calculated by fitting the RLCs on a nonlinear model by using a two-level hierarchical Bayesian model. The experimental results showed that Kappaphycus sp. required temperatures ranging from 26oC to 34oC to maintain their high levels of photosynthetic activity. Saturating irradiace (Ek) at the temperature range occured ranging from 120 to 150 μmol photons m-2 s-1. The model equation that have been derived from this series experiment can be used to determine the requirement of temperature and light intensity (irradiance) of any seaweed species.
EFFECT OF INBREEDING ON MORPHOLOGICAL TRAITS OF POPULATIONS OF GIANT FRESH WATER PRAWN, Macrobrachium rosenbergii: IMPLICATIONS FOR SELECTIVE BREEDING Imron, Imron; Wuwungan, Harry; Soelistyawati, Dinar Tri
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.737 KB) | DOI: 10.15578/iaj.8.2.2013.127-134

Abstract

Genetic improvement through selective breeding relies on the genetically-controlled phenotypic variability in the character of interest. Therefore, the extent of phenotypic variability in the population to be selected is an important parameter, as it potentially influences the population’s response to selection. This study was aimed to investigate the effect of inbreeding on the survivability, growth, and phenotypic variability of giant freshwater prawn (GFP), Macrobrachium rosenbergii. Two GFP populations of different inbreeding levels, namely 25% and 0%, respectively, were formed by mating of individual broodstock with known pedigree. Study was conducted for two months starting from newly hatched larvae up to nursery stage (30 day-old post larva). Phenotypic variability profile, expressed in the morphometric mean and coefficient of variation (CV) of twenty one morphometric characters were measured and evaluated. Results showed that in general, the inbred populations had lower values in the mean of all characters (100%), indicating that they suffered from inbreeding depression. Similarly, a lower CV values were observed in sixteen (75%) of the morphometric characters measured, indicating a potential reduced of genetic gain when they are used in selective breeding program. These results suggest the importance of controlling inbreeding level in breeding population that adverse effects resulted resulted from inbreeding can be minimized.
REMOTE SENSING AND GIS APPROACHES TOWARD SUSTAINABLE MANAGEMENT OF MARINE AQUACULTURE IN INDONESIA Radiarta, I Nyoman
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.8.2.2013.173-179

Abstract

The sustainable marine aquaculture (mariculture) has become increasingly important with the dramatic growth of the sector. To ensure long-term sustainability, planning is an important process that will stimulate and guide the evaluation of the sector. Many of the mariculture issues are entirely spatial in nature (e.g. siting, zoning), or have important spatial elements (potential mariculture areas, impact of mariculture, competition for space with other users). Satellite remote sensing integrated with geographic information systems (GIS) can play a major role in all geographic and spatial aspects of the development and management of mariculture. The potential capabilities of evolving GIS and remote sensing for the sustainability of mariculture provide a powerful tool for the efficient and cost effective management. These technologies are also useful for facilitating the decision making process in relation to mariculture. In this paper attempt to simply describe a number of ways in which GIS and remote sensing could be usefully employed as an aid to support sustainable mariculture development.
INDUCED FUCTIONAL MALE OF CORAL TROUT GROUPER (Plectropomus leopardus) USING 17α-METHYLTESTOSTERONE HORMONE Sembiring, Sar Budi Moria; Hutapea, Jhon Harianto; Wardana, Ida Komang; Muzaki, Ahmad; Mastuti, Indah
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.271 KB) | DOI: 10.15578/iaj.8.2.2013.101-106

Abstract

The success of grouper seeds production is depends on the availability of qualified broodstock. The nature of grouper is protogynous hermaphrodite, causing difficulties to mantain female and a bit difficult to get male broodstock, one possibility to accelerate sex revers is by hormone manipulation. The aim of this experiment was to find effectiveness of 17α-methyl testosterone hormone to produce coral trout grouper (Plectropomus leopardus) functional male. The experiment was conducted in floating net cage by using 6 net cages with size of 2 m x 2 m x 2 m at density of 25 fish/cage, size of fish were 377.27±21.49 g. The fishes were treated by hormone implantation at concentration of 50 μg/kg body weight and without hormone implantation as a control with 3 replicates. The results showed that the highest concentration of testosteron in fish blood (1.144±0.135 pg/mL) was detected after four months of hormone treatment, but the concentration of testosteron in fish blood declined after 8th months of treatment. The treated fish with hormone grew faster than control. Based on histological analysis of gonad, female gonado somatic index was higher for treated fish compare to control. Its seems that hormone tratment lead to promote development of female maturity and than sex reverse into male become faster.

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