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INDONESIA
Indonesian Aquaculture Journal
ISSN : 02150883     EISSN : 25026577     DOI : -
Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.
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Articles 9 Documents
Search results for , issue " Vol 7, No 1 (2012): (June 2012)" : 9 Documents clear
DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER Marnis, Huria; Dewi, Raden Roro Sri Pudji Sinarni; Imron, Imron; Iswanto, Bambang
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.545 KB) | DOI: 10.15578/iaj.7.1.2012.11-18

Abstract

Faster growing African catfish can be produced by transgenesis. This study was conducted to investigate the distribution and expression of growth hormone gene (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founder (F0). Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye. Transgene expression levels were analyzed using the method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish founder. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. The expression levels of PhGH had positive correlation with tissue and body size (P<0.05).
DISTRIBUTION ANALYSIS OF ENLARGED CELLS DERIVED FROM GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) INFECTED HUMPBACK GROUPER Cromileptes altivelis Mastuti, Indah; Mahardika, Ketut
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1548.747 KB) | DOI: 10.15578/iaj.7.1.2012.55-60

Abstract

Characteristic of Megalocytivirus infection has been known to produce formation of inclusion body bearing cells (IBCs) on internals organs of fish predominantly on spleen and kidney. Megalocytivirus that infected grouper is known as Grouper Sleepy Disease Iridovirus (GSDIV). This study was conducted to answer the effect of entry sites of GSDIV on distribution of enlarged cells formed on the internal organs of humpback grouper Cromileptes altivelis. Enlarged cells were observed histologically under the light microscope on spleen, head kidney, trunk kidney, liver, gill, heart, stomach, intestine, muscle and brain. Entry sites were designated to intramuscularly injection, intraperitoneally injection, dipped gill and inoculum added feed. Enlarged cells were formed on spleen, head kidney, trunk kidney, liver, gill, heart, stomach, muscle, except on intestine and brain. All the entry sites resulted in formation of enlarged cells on spleen, head kidney, trunk kidney, liver, heart. Spleen and head kidney were the most frequent observed organ. These results suggested that distribution of enlarged cells were not affected by the entry site of GSDIV.
NUTRITIVE COMPOSITION OF RED TILAPIA REARED IN FRESHWATER AND BRACKISHWATER Dewi, Raden Roro Sri Pudji Sinarni; Setyawan, Priadi; Tahapari, Evi; Robisalmi, Adam; Listiyowati, Nunuk
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.372 KB) | DOI: 10.15578/iaj.7.1.2012.19-27

Abstract

The aim of this research was to investigate the nutritive composition (especially fatty acids) in red tilapia that was reared in freshwater and brackishwater. The fatty acid contents were determined by gas chromatography. The fatty acids profile were -3 (linolenic acid, eicosapentaenoic acid/EPA, docosahexaenoic acid/DHA), -6 (linoleic acid, arachidonic acid/AA), and -9 (oleic acid). Red tilapia samples were obtained from Research Institute for Fish Breeding, Sukamandi, West Java (freshwater ponds) and Congot, Yogyakarta (brackishwater ponds; salinity 20 ppt). In this research, red tilapia reared in different ecosystems showed different fatty acid profiles. Red tilapia inhabiting brackishwater ecosystem has EPA (0.26±0.05%), DHA (3.42±0.26%), and linoleic acid (17.20±0.56%) content higher than freshwater ecosystem (EPA = 0%; DHA = 0.67±0.44%; linoleic acid = 9.08±4.76%), except for linolenic acid (0.30±0.15% vs 0.25±0.10%), arachidonic acid (0.77±0.39% vs 0.93±0.13%) and oleic acid (38.67±2.58% vs 37.44±0.74%). The ratio of -6/-3 in red tilapia inhabiting freshwater ecosystem was about 11/1. The culture tilapia in brackishwater ecosystem decrease -6/-3 ratio (4.5:1). So that for human health, it will be better to consume brackishwater red tilapia than freshwater red tilapia.
SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV) Mahardika, Ketut; Mastuti, Indah
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.7.1.2012.61-67

Abstract

RSIV is an isolate virus in the genus Megalocytivirus (family Iridoviridae) that has been reported to be pathogen in more than 31 marine fish species in East Asia. The aim of study was to know the susceptibility of several cultured cells to RSIV. RSIV inoculum was inoculated onto cultured cells and then incubated in 25oC. Routine observation of cytopatic effect (CPE) was carried out for 7 days and harvested cells were prepared for virus titration and electron microscopy (EM). The result showed that RSIV grew and propagated in GF (grunt fin), KF-1 (koi fin) and BF-2 (barfin flounder) which caused cytophatic effect as cel ls enlargement. However, RSIV did not propagated on EPC (epithelioma papulosum cyprini), FHM (feathed minnow) and EK-1 (eel kidney) cells. The virus titer were 105.3 TCID50/mL in GF cells, 103.8 and 4.3 TCID50/mL in KF-1, 103.6 and 3.8 TCID50/mL in BF-2, and 7 102.1 TCID50/mL in EPC, FHM and EK-1. The EM observation revealed formation of enlarged cells containing hexagonal virus particles with 140-160 nm in diameter. These results indicated that GF was cultured cell to be optimal for replication of isolate RSIV derived from Ise bay, Mie, Japan.
EFFECT OF CALCIUM SUPPLEMENTATION ON GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) MOULTING AND EGG QUALITY Khasani, Ikhsan; Ridzwan, Narita Syawalia; Jones, Clive
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.135 KB) | DOI: 10.15578/iaj.7.1.2012.29-36

Abstract

A breeding program for giant freshwater prawn has been developed in Indonesia to supply good quality prawn seed to industry. To achieve the goal of the program, optimum conditions of both environment and nutrition must be provided. Calcium is the main compound of the prawn carapace, influencing moulting processes, especially during the premating moult. The effect of calcium supplementation in the prawn feed on moulting process and egg quality of giant freshwater prawn, Macrobrachium rosenbergii, was investigated. Experimental units consisted of 300 L plastic tanks stocked with 4 adult prawns comprising 3 female and 1 male. A standard prawn feed containing 30.39% crude protein; 0.82% Ca and 0.47% P was used as the experimental diet. A complete randomized design was‘applied in the study with 5 dosage levels of dicalcium phosphate i.e. 0%, 2%, 4%, 6%, and 8% with 4 replicates. Prawns were reared over 45 days. There were significant differences (P<0.05) in premoult, moult, and egg hatching rate, while no significant differences in intermoult or fecundity were observed (P>0.05). Egg hatching rate increased liniearly from 26.5%±9.9% to 50.8%±10.3% as calcium dosage increased from 0% to 8%. The number of eggs per spawn was not significantly different (P>0.05), ranging from 40,096 to 46,131 for females weighing 30.19 to 32.94 g. The results of this study suggest that giant freshwater prawns require dietary calcium supplementation to support moulting process and egg quality when reared in soft water.
THE DYNAMIC OF LANDSCAPE CHANGE AT COAST AREA, IN LABAKKANG SUBDISTRICT PANGKEP REGENCY, SOUTH SULAWESI Tantu, Andi Gusti; Soemarno, Soemarno; Harahab, Nuddin; Mustafa, Akhmad
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.294 KB) | DOI: 10.15578/iaj.7.1.2012.69-77

Abstract

Development is a changing process to improve human standard of life that strongly related to the activities of natural resource utilization. It is frequently found that these activities change the ecosystems and their resources. Eventually, these changes wil l give ser ious impacts to the envi ronments. The most distinct environmental problems are caused by resident migration to the coastal areas, coastal developments, and land limitation. Coastal reclamation is an example of human effort to respond to the land limitation in the coastal areas as observed in coastal areas of Labakkang Subdistrict. Reclamation activity is doing along the coast of Pundata Baji Village, whereas, in other villages, cutting off of mangrove areas as an alternative land expansion is increasingly practiced by the local communities. This Research was aimed to observe the landscape change from 1980 to 2010 at coast area in Labakkang Subdistrict. Each review considers Landsat Image Map (acquired in 1980); Landsat Image Map (acquired in 1990); Landsat Image Map-7 Enhanced Thematic Mapper Plus (ETM+) acquired in 2000); Spot Image 4 (acquired in 2005); and Spot 4 LAPAN (acquired in 2010). Result of research indicates that at coast area of Labakkang Subdistrict in 1980, there are 248.3 ha of mangrove vegetation, 2,756.63 ha inundation, and 4,157.0 ha open land. In 1990, there are 234.2 ha mangrove vegetation, 2,251.63 ha embankment, 933.2 ha rice field, and 582.0 ha open land. In 2000, there are 218.3 ha mangrove vegetation, 2,848.1 ha embankment, and 3,579.2 ha rice field. In 2005, it has 121.4 ha mangrove vegetation, 3,762.6 ha embankment, and 2,306.2 ha rice field. In 2010, it is found 48.9 ha mangrove vegetation, 5,029.35 ha embankment, and 749.98 ha rice field.
INFLUENCE OF FISH MEAL REDUCTION, ALGAE ADDITION, AND ENZYME USE IN SHRIMP FEEDS ON THE TISSUE COMPOSITION OF Litopenaeus vannamei Kaspers, Sebastian; Kunzmann, Andreas
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (959.051 KB) | DOI: 10.15578/iaj.7.1.2012.37-47

Abstract

At the Leibniz-Center for Tropical Marine Ecology in Bremen, Germany, shrimps (Litopenaeus vannamei) fed feeds with reduced fish meal content, algae and enzyme additions were analysed for their tissue composition. Contents of protein, glycogen, total lipid, linoleic acid (LA), alpha linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of the shrimp abdomen and of the respective feeds were determined. Reduction of fish meal (FM) content in shrimp feed and its substitution with “mash” (grain distillery waste-”Schlempe”), corn gluten, pea or coarse colza meal did not influence the tissue composition. Additional inclusion of an algae mixture out of Spirulina spp., Phaeodactylum spp., and Tetraselmis spp. to shrimp feeds as well as the single inclusions of Phaeodactylum spp. or Spirulina spp. had no effect on the tissue composition. Furthermore no improvement of the quality of the shrimps due to an enhanced digestibility of mash by enzymes (mixture of corolase, phytase, xylanase) could be detected. In contrast the quality of shrimps seemed to be negatively affected by enzymes in this study. These findings could contribute to making shrimp aqua farming more economic as reduction of feed costs without reduction of the quality of the shrimps would be a large benefit.
GENETIC VARIABILITY OF THREE POPULATIONS OF FLYING FISH, Hirundichthy oxycephalus FROM MAKASSAR STRAIT Parenrengi, Andi; Tenriulo, Andi; Ali, Syamsul Alam
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.605 KB) | DOI: 10.15578/iaj.7.1.2012.1-10

Abstract

Flying fish, Hirundichthy oxycephalus is one of economically important marine species to Indonesia, particularly in Makassar Strait and Flores Sea. However, there is a limited published data on genetic variation in molecular marker level of this species. Random Amplified Polymorphic DNA (RAPD) was employed in this study to determine the genetic variability of three populations of flying fish collected from Takalar, Pare-Pare, and Majene in Makassar Strait. Genomic DNA was isolated from preserved muscle tissue using phenol-chloroform technique. Two selected arbitrary primers (CA-01 and P-40) were performed to generate RAPD finger printing of flying fish populations. The two primers generated a total of 81 fragments (loci) and 50 polymorphic fragments with size ranging from 125 to 1,250 bp. There were no significant differences in number of fragment and number of polymorphic fragment among populations. The high polymorphism (63.5±7.4%) was obtained from Takalar population followed by Pare-Pare (58.3±19.6%) and Majene population (57.7±0.8%). Similarity index of individuals was 0.60±0.17 for Takalar, 0.63±0.17 for Majene and 0.75±0.21 for Pare-Pare population. Seven fragments were identified as species-specific markers of H. oxycephalus. The UPGMA cluster analysis showed that the Takalar population was genetically closer to Pare-Pare population (D= 0.0812) than to Majene population (D= 0.1873).
FATTY ACID COMPOSITION AND PROSTAGLANDIN CONTENT OF THE RED SEAWEED Gracilaria sp. FROM INDONESIA Illijas, Muhammad Ikbal; Arifuddin, Arifuddin; Saleh, Luqman; Itabashi, Yutaka
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.974 KB) | DOI: 10.15578/iaj.7.1.2012.49-54

Abstract

High content of polyunsaturated fatty acids (PUFAs) such as arachidonic and eicosapentaenoic acids are typical for the red alga. Analysis of fatty acid composition and prostaglandin content was conducted in the red alga Gracilaria sp. from Indonesia. Total lipid of the alga was extracted with CHCl3-MeOH (2:1, v/v). Analysis of the fatty acids composition was performed on gas chromatography (GC) equipped with omega wax column (30 m x 0,32 mm i.d., Supelco, PA, USA) and analysis of prostaglandins were carried out by HPLC on ODS column (Mightysil RP-18 GP, 250 mm x 4.6 mm, 5 μm). The content of fatty acids high for were palmitic acid (50%) and arachidonic acid (26.9%), whereas prostaglandin E2 was identified and found lower concentration (44.2 μg/gram total lipid).

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