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INDONESIA
Indonesian Aquaculture Journal
ISSN : 02150883     EISSN : 25026577     DOI : -
Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.
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Articles 9 Documents
Search results for , issue " Vol 4, No 1 (2009): (June 2009)" : 9 Documents clear
ASSESSMENT OF GENETIC VARIATION OF PEARL OYSTER, Pinctada maxima, BASED ON THE ANALYSIS OF MITOCHONDRIAL CYTOCHROME OXIDASE SUBUNIT I GENE Sudradjat, Achmad; Susilowati, Rini; Imron, Imron
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.663 KB) | DOI: 10.15578/iaj.4.1.2009.19-24

Abstract

Pearl oyster, Pinctada maxima is one of economical ly important species in aquaculture, particularly in pearl industry. Information on genetic variation of pearl oyster is required in order to be able to make a sound management of it’s natural populations and to utilize it to improve the quality of pearl culture. Five populations from different geographic locations of pearl oyster, Pinctada maxima, (Sumbawa, Bali, Selat Sunda, Belitung, and South Sulawesi) were analyzed for genetic variation within a 750-base pair region of the Mitochondrial Cytochrome Oxidase subunit I (MtCOI) gene using Restriction Fragment Length Polymorphism (RFLP) technique. The analysis of 25 pearl oyster samples, their haplotype diversity ranged from 0.0970 to 0.1939 and the number of haplotype in each population ranged from three to five haplotypes. Clustering of populations based on Nei’s genetic distances and constructed using unweighted pair-group method with Arithmetic mean (UPGMA) showed that the populations were clustered into two groups: Belitung, Selat Sunda, Bali and Sumbawa in one group, while South Sulawesi in the second group.
ELECTRON MICROSCOPIC STUDY ON ENLARGED CELLS OF RED SEA BREAM, Pagrus major INFECTED BY THE RED SEA BREAM IRIDOVIRUS (RSIV, GENUS Megalocytivirus, FAMILY Iridoviridae) Mahardika, Ketut
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1450.737 KB) | DOI: 10.15578/iaj.4.1.2009.53-63

Abstract

Most histopathologycal studies of the red sea bream iridovirus (RSIV) disease in red sea bream have been performed by studying enlarged cells as well as necrotized cells in the spleen and other organs. These enlarged cells have been named as inclusion body bearing cells (IBCs). However, few information is available about detail of ultrastructural features of IBCs produced in the target organs of RSIV-infected fish. In the present study, details of ultrastructural features of IBCs that were produced in the spleen tissue of naturally RSIV-infected red sea bream were investigated under electron microscope. Under electron microscope, RSIV-infected red sea bream had the presence of two types of IBCs: typical IBCs allowing virus assembly within viral assembly site (VAS), and atypical IBCs which degenerate organelles without virus assembly. Other infected-cells were observed as necrotized cells forming intracytoplasmic VAS with large numbers of virions, but without the formation of the distinct inclusion body. Morphogenesis steps on RSIV-infected red sea bream were observed as filamentous-filed virions, partially-filled virions and complete virions with 145-150 nm in size. These findings confirmed that RSIV-infected red sea bream were characterized by formation of typical and atypical IBCs as well as necrotized cells.
THE EFFECT OF INBREEDING ON THE EARLY PERFORMANCE OF FRESHWATER PRAWN, Macrobrachium rosenbergii Imron, Imron
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.18 KB) | DOI: 10.15578/iaj.4.1.2009.25-31

Abstract

Inbreeding depression has often been considered to be responsible for the deterioration of performance in aquaculture species. Despite a crucial impact that may result from inbreeding depression, comprehensive information reviewing this subject is limited. This study was aimed to gain information on the effect of inbreeding on the early performance of freshwater prawn. The study was performed by comparing performance of inbred and outbred populations. Inbred population was established by brother-sister mating (inbreeding rate of 25%) while the outbred population was formed by mating unrelated individuals. Several fitness and productivity related traits including survival, the rate of larval development, stage dispersion and growth of larvae were evaluated. Results suggest that inbred families performed poorer than that of the outbred in survival. However, inbreeding depression did not seem to occur in other traits including the rate of larval development, larval stage dispersion and growth. This study implies that to maintain genetic quality of farmed prawn stocks, inbreeding rate in farmed population must be controlled not to exceed that level. Implications that these findings may have on aquaculture practices and possible alternatives for the solutions are discussed.
ARE COPEPODS VIABLE OPTIONS AS LIVE FOOD IN AQUACULTURE HATCHERIES? Iba, Wa; Albasri, Hatim
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.76 KB) | DOI: 10.15578/iaj.4.1.2009.65-73

Abstract

This present paper overviews the use of copepods in aquaculture. Some culture methods and nutritional values also described to decide whether copepods are viable and reliable to be used as live food in aquaculture hatcheries. Copepods have been known to have higher nutritional value than Artemia and rotifers. In aquaculture, they have been used to fed various species of marine finfish with better results in terms of growth, larval survival and pigmentation compared to some fish larvae fed on other live feeds. However, culturing copepods in intensive systems to harvest high number of copepods is not well established yet due to lack of funding and knowledge. Meanwhile extensive and semi intensive systems are possible to transfer parasites and diseases from wild environment. Furthermore, nutritional value can not be controlled in such systems.
THE EFFECTS OF DIFFERENT LEVELS OF DIETARY PROTEIN AND LIPID ON THE GROWTH OF RED SNAPPER, Lutjanus sebae Giri, Nyoman Adiasmara; Suwirya, Ketut; Marzuqi, Muhammad; Astuti, Ni Wayan Widya
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (873.112 KB) | DOI: 10.15578/iaj.4.1.2009.33-40

Abstract

Red snapper, Lutjanus sebae is favored in mariculture activities because it has a relatively good market and price. Technology for big scale seed production of this species has been developed and is now adequate to supply seed for grow-out activities. However, the availability of artifical diets for L. sebae is still a major constraint for grow-out production. Data on optimum dietary protein and lipid requirements for this fish as a basic information in feed development is not available yet. The objective of the present study was to find out dietary protein and lipid requirements for juvenile of L. sebae. A 70-day feeding experiment was conducted in 24 fiberglass tanks, 200 L volume. Each tank was equipped with a flow-through water system. Twenty five hatchery-produced juveniles of L. sebae (43.1 g BW) were randomly selected and stocked in each tank. The fish were fed with the experimental diets twice everyday at a level of 3% of biomass for the first 4 weeks, and then 2% of biomass afterward. Twelve experimental diets were prepared in form of dry pellet containing casein and fish meal as the main protein sources. Experimental diet had 4 levels of crude protein (32%, 37%, 42%, and 47%) and each protein level consisted of 3 levels of lipid (7%, 12%, and 17%). The experiment employed factorial method with completely random design using 12 combination treatments and 2 replications for each treatment. Result of the experiment showed that there was no significant effect of dietary protein and lipid on growth, feed consumption, and feed efficiency of tested fish. Growth and feed efficiency of fish fed on diet containing 42% and 47% crude protein were significantly higher than that of fish fed on diet containing 32% and 37% crude protein. High lipid content in the diet (17%) resulted in poor growth and poor feed efficiency. This data indicates that Lutjanus sebae has limited ability to utilize dietary lipid as an energy source. Result of the present study recommends that dietary protein and lipid requirement for good growth of L. sebae should be 42% and 12%, respectively.
CLONING OF ProAV PROMOTER ISOLATED FROM TIGER PRAWN Penaeus monodon Parenrengi, Andi; Alimuddin, Alimuddin; Sukenda, Sukenda; Sumantadinata, Komar; Yamin, Muhammad; Tenriulo, Andi
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.086 KB) | DOI: 10.15578/iaj.4.1.2009.1-7

Abstract

Promoter is a specific DNA sequence involved in the transcription of a particular gene. It is usually located in the upstream of the gene they regulate. Isolation and characterization of promoter is essentially needed in order to establish the sequence analysis and transcription factor that are used in the regulation of gene expression. The research was conducted to analyze the characteristics of Penaeus monodon anti viral gene promoter (ProAV) towards generation of auto-transgenic tiger prawn, P. monodon. ProAV promoter was isolated by PCR (Polymerase Chain Reaction) method and the purified DNA fragment was cloned into pGEM-T Easy cloning vector. The promoter sequence was characterized by using BLAST-N and Genetyx version 7 softwares. The results showed the success in isolating a promoter from tiger prawn of 368 bp in length. BLAST-N analysis showed that the sequence of isolated promoter has high similarity (95%-98%) compared to the other promoters in the GeneBank. The study revealed the existence of important transcription factors (TATA box, MRE, TCF-1, and other potential regulatory elements) are identified in the promoter sequence.
THE POTENTIAL OF EXTRACT OF LEAVES AND FLOWERS OF Lantana camara Linn. AS AN ANTIBACTERIAL FOR CATFISH (Clarias gariepinus) INFECTED BY Aeromonas hydrophila Nur, Indriyani; Fitriani, Afiyfah; Asnani, Asnani
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (36.924 KB) | DOI: 10.15578/iaj.4.1.2009.41-45

Abstract

Freshwater catfish culture has been hampered by bacterial diseases. One of the agents of the bacterial disease is Motile Aeromonas Septicemia (MAS). The application of synthetic antibiotics has had some disadvantages such as bacterial resistance and undegradable in water. One of the potential antibacterial herbs is Lantana camara. Information of Lantana as an antibacterial on catfish is still limited. Therefore, the experiment of utilization of Lantana as an antibacterial for catfish should be conducted. The experiment was carried out to evaluate the potential of Lantana extract as an antibacterial of A. hydrophila for catfish. The completely randomized design was applied consisting of four treatments using two parts of the plant, leaves and flowers. The treatments were: A = 1,000 ppm of leaves; B = 2,000 ppm of leaves; C = 1,000 ppm of flowers; D = 2,000 ppm of flowers), and control. Lantana extracts were diluted into each culture media which had been infected with A. hydrophila. Several factors were observed in this experiment such as prevalence with of MAS disease, survival rate, percentage of haematocrites and total of leukocytes of fish blood. The results showed that the fish treated with 2,000 ppm of flowers extract had a lower in prevalence of MAS disease and higher in survival rate than those treated with 1,000 ppm; 2,000 ppm of leaves; and 1,000 ppm of flowers, respectively. However, percentage of haematocrytes and total of leucocytes was not influenced by the extracts from different parts of Lantana plant. In conclusion, 2,000 ppm of Lantana flowers extract might be useful as an antibacterial of A. hydrophila for catfish culture.
IDENTIFICATION OF GROWTH HORMONE GENE OF Pangasionodon hypophthalmus Dewi, Raden Roro Sri Pudji Sinarni; Sudrajat, Agus Oman; Alimuddin, Alimuddin; Sumantadinata, Komar
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.557 KB) | DOI: 10.15578/iaj.4.1.2009.9-17

Abstract

Identification of growth hormone (GH) gene in a target fish is the first step in the construction of “all fish genes transfer vector” to generate transgenic fish. The research was done to identify and characterize the GH gene of Pangasionodon hypophthalmus. There were several activities performed in identifying the GH gene: RNA extraction, cDNA synthesis, PCR amplification, and DNA fragment isolation. The characterizations were done using the nucleotide sequencing engine ABIPRISM 3100. The results were then analyzed using BLASTN/P and GENETYX version 7 program. The full-length GH gene of P. hypophthalmus was 1151 bp in length, coding for an open reading frame (ORF) of 603 bp. The 5’ and 3’ untranslated regions of the GH gene were 22 bp and 526 bp long, respectively. The GH gene of P. hypophthalmus had some common characteristics that are owned by GH genes, such as single tryptophan residue (W) on the 104th amino acid, 5 cysteine residues (C) on the amino acid 71, 135, 173, 190, and 198 and a motif of Asn-Xaa-Thr on C terminus which is the potential location for N-linked glycosilation. Polyadenylation signal (aataaa) was on the 14 bp at the upstream of polyadenylation location. Growth hormone of P. hypophthalmus consisted of over 200 amino acids from GH cDNA deduction. The highest proportion of amino acid composition was leusin (14%) while the lowest was tryptophan (0.5%).
Streptococcus agalactiae INFECTION ON TILAPIA (Oreochromis niloticus) IN CIRATA RESERVOIR, WEST JAVA Lusiastuti, Angela Mariana; Gardenia, Lila; Mufidah, Tatik; Aryati, Yani
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
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Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.069 KB) | DOI: 10.15578/iaj.4.1.2009.47-51

Abstract

Streptococcosis is one of bacterial diseases in the culture of Tilapia, Oreochromis niloticus and has caused significant economic losses. Streptococcus iniae, is known as pathogen to marine and freshwater fishes whereas Streptococcus agalactiae is known as pathogen to Tilapia. The isolation and characterization of four isolates of S. agalactiae, were described from an infected Tilapia from Cirata Reservoir, West Java, in July 2008. Conventional and rapid identification systems were used to determine isolates of S. agalactiae from brain and kidney tissues. In this paper, we have characterized S. agalactiae and this was the first isolation of this bacteria from fish. The isolates were gram positive, catalase-negative, oxidase-negative,  haemolytic cocci colonies on blood agar. All of the of isolates were biochemically characterized with the API 20 Strep System (bioMerieux). Bacterial chromosomal DNA used in PCR assay was extracted by heating method. The forward primer is Sdi 61: 5’-AGGAAACCTGCCATTTGCG-3’ and the reverse primer is Sdi 252: 5’-CAATCTATTTCTAGATCGTGG-3’ with gene target 16S intergenic spacer and it has 192 bp in length. These primers were designed by Alpha DNA (Montreal, Quebec). The biochemical patterns of four isolates were rather different although almost all traits were similar with the exception of pyroglutamic acid (pyra) and L-arginin (ADH), for which we observed negative and positive reaction in this study. Therefore, some of the biochemical characteristics of the four isolates did not fit 100% with the typical patterns of S. agalactiae. However, the PCR result showed that this PCR assay is an effective tool for rapid and specific detection of S. agalactiae, the main pathogens involved in warm-water streptococcosis, obtained from pure culture of naturally infected fish. Therefore, it could be a useful alternative for culture-based routine diagnosis of warm-water streptococcal infections in fish.

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