Abstrak Traditional products derived from the local wisdom of the Tarakan City coastal communities, namely in the form of cold protective skin powder from the sun which is made from mangrove raw Sonneratia alba. This product has potential to be developed to improve the economic value of local communities. They can compete with products other sunscreen products produced by industry (commercial) through cream preparation technology (sunscreen).This research was carried out for seven months (February-August 2018) in the Environmental Laboratory of FPIK UBT, Samarinda Pharmacy laboratory (AKFARSAM), and the UNMUL Mathematics and Biochemistry laboratory. The research procedure used maserasi method with 70% ethanol solvent followed by concentrating the extract using a rotary evaporator to obtain extracts of active ingredients and carried out qualitative phytochemical testing. The obtained active ingredient was is then formulated into a sunscreen cream preparation. The level of protection of mixed sunscreen cold powder and commercial cream were tested by using UV-VIS spectrophotometer to see the value of Sun Protection Factor (SPF) at absorbance of 290-320 nm. Qualitative phytochemical test showed that on S.alba contains of positive alkaloids, phenols, flavonoids, steroids, and tannins, and negative saponins. ANOVA statistical analysis (95%) based on the calculated F value
GIMacro II prawn larvae is able to grow well at a salinity of 8-15 ppt. This condition can be improved by improving the methods of adaptation to changes in salinity prawn larvae production activities, by determining the pattern of changes in salinity are right. Salinity media through osmotic pressure affect the physiological activity, where the cells in body organs prawns should be in liquid media with ionic composition and concentration of the same with the environment. Having obtained the optimum salinity on larval rearing prawns GIMacro II in different salinity media is expected to produce a population of prawns GIMacro II with superior durability specific to environmental conditions, which can then be directed to improve the utilization of marine resources to the salinity of the best. This research aims were to study the growth and survival of larvae prawns GIMacro II reared on media of different salinities. The study used completely randomized design with three treatments and three replications. The treatments were larval rearing prawns GIMacro II at different salinities (10 ppt, 12 ppt, and 14 ppt). The results showed that the culture of prawn larvae GIMacro II at different salinity affect significantly on growth and survival of larvae prawns GIMacro II. The highest development of GIMacro II prawn larvae obtained from 12 ppt salinity treatment (7.13 ± 0.03%) and the highest survival rate was obtained also from 12 ppt salinity treatment (67.67 ± 4.51%)
Isolate D2.2 is a bacterial isolate with 97% homology level with Bacillus sp. This isolate is a biocontrol bacterium capable of inhibiting the growth of Gram positive bacteria and Gram negative bacteria. The use of biocontrol bacteria is one solution of disease problem in cultivation organism. Prior to being applied to the field, D2.2 isolates need to go through a series of tests, one of which is growth kinetics testing under various environmental conditions, such as different degrees of pH and salinity. This is because environmental factors can affect the rapid growth of bacteria. The purpose of this study was to study the bacterial growth kinetics of D2.2 at different pH and salinity. The growth kinetics was observed by measuring optical density (OD) through a method of turbidimetry using a spectrophotometer at a wavelength of 625 nm to the stage of death. The results showed that the fastest growth rate was found at 20 ppt salinity with 0.179 h-1 and generation time of 5,588 hours. While in all pH treatments, generation time and growth rates achieved all treatments have the same pattern.
Catfish (Pangasius sp.) Is one of the most cultivated freshwater fish, because it is one of the fish that has a high economic value. However, the use of phytopharmaca is used to improve feed efficiency and fish growth. One of the phytopharmaca which can be used as an antimicrobial is temulawak (Curcuma xanthorrhiza Roxb). This study aims to determine the optimal dose of the effect of temulawak extract on artificial feed in increasing feed consumption, efficiency of feed utilization, and growth rate of catfish (Pangasius sp.). The test fish is maintained with a stocking density of 1 tail / l with a maintenance time of 42 days. This study used an experimental method with 4 treatments and 3 replications. The treatments in this study were Treatment A (addition of 0 ml ginger extract), B (addition of 6 ml temulawak extract), C (addition of 12 ml curcuma extract), and D (addition of 18 ml ginger extract). The data observed included absolute weight, crime scene, EPP, SGR, REP, SR and water quality. The results showed that the addition of temulawak extract to artificial feed had a very significant effect on absolute weights, crime scenes, EPP, SGR, REP but did not differ significantly for survival. The optimum dose of addition of curcuma extract to total feed consumption is 11 ml capable of producing a maximum crime scene of 168.1 g, efﬁciency of feed utilization is 10.5 ml capable of producing a maximum EPP of 70.3% and specific growth rate is 10.8 ml resulting in maximum SGR 2.46% / day
Contamination of pathogenic and spoilage bacteria on fermentation process of seafood and fisheries product is a major concern on food safety. The aims of this study were isolating and applying the bacteriocin- and protease-producing lactic acid bacteria from the gastrointestinal tract of mud crab for starter culture of masin, a traditional fermented shrimp from East Java. This study consisted of characterization of lactic acid bacteria, the application on the fermentation process and microbial analysis. Ninety-four isolates were isolated from mud crab was screened for the bacteriocin and protease producing as well as characterized by pH, salinity and biochemical. Isolate IKP-29 was exhibited strong protease and bacteriocin activity. Application of Isolate IKP-29 on masin fermentation showed that sharp reduction of Escherichia coli, Vibrio sp. and lactic acid bacteria counted. This study suggested that the application of lactic acid bacteria which producing bacteriocin and protease improved the food safety of traditional fermented fish.
Vaname shrimp (Litopenaeus vannamei) has high economical value as an export commodity However, there are obstacles that cause a decline in the level of shrimp exports in the world. One of the obstacle is shrimp disease which is treated using antibiotics. This method might ca-used pathogenic resistance and become a residue when consumed by humans. Avi-cennia sp. fruit extracthas benefits as natu-ral antibacterial ingredient that are safe to treat the shrimp which is infected by the Vibrio parahaemolyticus. In this study, shrimp is infected with Vibrio parahaemo-lyticus immersed using Avicennia sp. with a concentration of 300 mg L-1, 350 mg L-1, and 400 mg L-1 for 21 days plus the control treatment. The concentration of 400 mg L-1 showed better results compare to other con-centration on all observed parameters; fas-ter recovery time, higher survival rate and relative percent survival (RPS), also mild damage on hepatopancreas test. Keywords: Litopenaeus vannamei, antibiotics, Avicennia sp., fruit extract Vibrio parahaemolyticus