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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 7 Documents
Search results for , issue " Vol 34, No 3 (2017)" : 7 Documents clear
Perubahan Kadar Estradiol dan Histologi Uterus Mencit (Mus musculus) Betina dengan Induksi Progesteron Sintetik Narulita, Erlia; Prihatin, Jekti; Anam, Khoirul; Oktavia, Fikri Ainur Risma Hardiyanti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.487

Abstract

The purpose of this research was to determine the effect of progesterone to estradiol levels and histology of uterus, to test the effect of estrous cycle to uterus histology of females’ mice after treated by progesterone in birth control pills. This research used Completely Randomized Design (CRD). Females Mus musculus Balb-C and synthetic progesterone (cyproterone acetate) contained in ‘Diane-35’ birth control pills were used in this study. The research consisted of two treatment groups, i.e. group K had no treatment and group P treated by a solution of Diane-35 at a dose of 2.6 mg per 5 mL distilled water. Results on the effects of progesterone on endometrial thickness suggested that there were significant differences between treatment groups. Meanwhile, the effect of progesterone on estradiol levels indicated that there were no significant differences among treatment groups. This means that the thickness of the endometrium was strongly influenced by the presence of a synthetic progesterone hormone and induced endometrium growth thicker. The levels of estradiol are not significantly different due to a negative feedback mechanism of Follicle Stimulating Hormone (estrogen effect) and Luteinizing Hormone (progesterone effect). The greatest thickness of endometrium of estrous cycle was treated by progesterone, which was 0.2500 mm2.
Ekspresi mRNA BRLF1 Virus Epstein-Barr dari Biopsi Jaringan Tumor Formalin-Fixed Paraffin Embedeed sebagai Petanda Biologi Molekul Diagnosis Karsinoma Nasofaring Herkilini, Arin; Hendrati, Pancrasia Maria; Gumilas, Nur Signa Aini; Sulistyo, Hidayat
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.508

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor that grows in the nasopharynx with a predilection in the fossa Rosenmuller. Epithelial malignancies are often found in populations of China and Southeast Asia, including Indonesia. The NPC incidence in year 2008 as many as 84,400 cases and 51,600 of these cases resulted in death. A total of 120 new cases per year NPC found in hospitals Prof. dr. Margono Soekarjo (RSMS), Purwokerto. The NPC is difficult to be diagnose caused its primary tumor lies closed to the skull base as well as various structures of vital organs. Therefore, methods that can detect early NPC required for inspection.The etiology of NPC is multifactorial consisting of genetic factors, factors of infection Epstein-Barr Virus (EBV) and environmental factors.EBV has two phases in the cycle of infection that is the phase of lytic and latent phase.  BRLF1 has an important function as  mediator transition from latent e NPC. The research aimed  to analysis mRNA BRLF1 expression as a biomarker of  NPC diagnosis by RT-PCR and to determine the positivity of RT-PCR method to detect the expression of mRNA BRLF1. The research design was cross sectional study. Samples were FFPE tumor biopsy of NPC WHO III  and the total samples were 22 individu from Department of Pathology Anatomy,  Prof. Dr. Margono SoekarjoHospital, Purwokerto with informed consent. The positivity of mRNA BRLF1 from FFPE tumor biopsy of NPC WHO III  was in 63.6%indicating a high expression.
Pertumbuhan Rumput Gajah Kerdil (Pennisetum purpureum ‘Mott’) di Lahan Terbuka Bekas Penambangan Batu Kapur Kawasan Karst Gombong Jawa Tengah Sarwanto, Doso; Tuswati, Sari Eko
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.502

Abstract

The negative impact of limestone mining on a karst area around Gombong is the destruction of ecosystem. Ecosystem’s destruction will decrease the variety, productivity, quality and continuity of indigenous livestock forages. The aim of this research is to know the growth rate of Dwarf Elephant grass (Pennisetum purpureum Mott) in the post mining limestone with various methods of planting. The method used in this research is an experimental method in an open field that was used for limestone mining by using Completed Random Design. The treatment consists of 4 kinds of planting methods with 4 repetitions, while the research treatment used in this experiment is the RG (the field with Dwarf Elephant Grass); RGL (the field with Dwarf Elephant Grass + peanut legume); RGK (the field with Dwarf Elephant Grass + goat’s compost 1,5 kg/m2) and RGKL (the field with Dwarf Elephant Grass + peanut legume + goat’s compost 1,5 kg/m2). The parameter used for the research is the growth rate which includes the plant’s height, the length of the leaves, and also the width of Dwarf Elephant Grass’s leaves (Pennisetum purpureum ‘Mott’) until the 8th week.The result of this research shows that the use of goat’s compost 1,5 kg/m2 and planting of peanut can give significant results to the growth of Dwarf Elephant Grass in open field that was used for limestone mining.
Ekstraksi Na-Alginat dari Rumput Laut Padina sp. Menggunakan Konsentrasi Kalium Hidroksida yang Berbeda Septiani, Elza; Pratama, Ginanjar; Putri, Raja Marwita Sari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.500

Abstract

The objective of this study was to obtain the best sodium alginate extract from Padina sp. with different potassium hydroxide concentration. Padina sp. was collected in coastal Senggarang, Tanjungpinang, Riau Islands Province. The raw material of Padina sp. was extraction by potassium hydroxide with different concentration (0.6%, 0.8%, 1%).  We analyzed content of moisture, ash, yield, viscosity and pH. The best results obtained in the treatment with  a solution of KOH concentration 0,8% with content of moisture 10,87%, ash 31,24%, yield 17,82%, viscosity 275,50 cP and pH 8,21. 
Keanekaragaman Jenis Amfibi untuk Mendukung Kegiatan Ekowisata di Desa Braja Harjosari Kabupaten Lampung Timur Arista, Angga; Winarno, Gunardi Djoko; Hilmanto, Rudi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.458

Abstract

Amphibian life in nature is an interesting case which can be an ecotourism interest. The life cycle as well as the uniqe behaviours of the amphibi, is an interesting case to support the ecotourism activities. This research intends to determine the richness of amphibian minds, the diversity of amphibian kinds, and the amphibian distribution in order to support the ecotourism activities and to determine the society perception in utilizing the amphibi for the ecotourism activities. A combination of line transect method and Visual Encounter Survey method were used to know the species richness diversity and the distribution. One score one indicator quisioner was used to know the society perception against the amphibi to support ecotourism. The research was conducted in Braja Harjasari Village, East Lampung Regency during March to April 2017. Data analyzing was used a Shannon-wiener and Margalef index. The sosciety perception was analyzed descriptively and showed in a chart. The result showed that the amphibian richness was 0,6 which mean low, the diversity was 1,0 which meant medium, the shannon-wiener and margalef index was 0,9 which mean low community was stable and each kind of the amphibi was distributed equally. The society perception against the amphibi to support the ecotourism activities was mostly positive.
Uji Antagonisme Lentinus cladopus LC4 terhadap Ganoderma boninense Penyebab Penyakit Busuk Pangkal Batang Kelapa Sawit Angraini, Erni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.512

Abstract

The growth of oil palm can not be separated from disease attack. One of the diseases that attack is the Base Stem Rot disease caused by Ganoderma boninense. Efforts to control the pest and disease one of them by using Lentinus Cladopus LC4 biological controller so that the purpose of this study is to determine the potential of L.cladopus LC4 as biological controlling agent G. boninense. The method used is L. cladopus LC4 antagonism test against G. boninense. The results showed that L. cladopus LC4 had antagonistic potency against G. boninense pathogen, although the mechanism did not show any inhibition zone. Therefore, L.cladopus LC4 may be considered for the prevention and control of plant diseases in the field.
Biodisel dari Mangrove Jenis Nyamplung (Callophylum inophyllum) sebagai Alternatif Pengganti Bahan Bakar Minyak Fosil Suyono, Suyono; Hartanti, Ninik Umi; Wibowo, Agus; Narto, Narto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.505

Abstract

Resources of fossil fuel continue to decrease and cannot be renewed so that replacement alternative is needed. Biofuels (biodiesel) become one of the alternatives because it can be renewed and environmentally friendly. First-generation biodiesel from food sources and second generation of food waste can disrupt the availability of food. Those are inefficient because it requires extensive land for its provider. Seed of nyamplung (Callophylum inophyllum) is very potential as biodiesel raw material because of its oil content 40-70% is high compared to other types of plants, such as jatropha range of 40-60% and palm of 46-54%, raw materials saving and having twice of fuel as much as kerosene. The general purpose of this research is to produce third generation biodiesel from nyamplung  as material to overcome fossil fuel crisis especially for fishermen and farmers in coastal area of Brebes Regency and surrounding areas. To be able to achieve the goal / general target is done by assessing oil content in nyamplung seed, biodiesel synthesis, intensive culture techniques and models of development of mass culture. The biodiesel yield produced from nyamplung seed oil in this study was 82.87%, in the range of biodiesel yields of previous researchers through a single stage transesterification of 79.03-91,00%. Cetana number and alkyl ester figures in the resulting biodiesel respectively 79.5 and 99.71% have met the Indonesian National Standard (SNI). The resulting density was 893.1 kg / m3, fog point  was 20oC and total glycerol  was 0.26%, was approaching the SNI by an insignificant difference, while the acid number generated was 1.86 mg KOH/gr of biodiesel and the biodiesel viscosity was 15.7 mm2 /s has not fulfilled the SNI significantly. To improve the number of acids and viscosity is still needed refinement biodiesel synthesis process through the diversification of the number of esterification stages, alternative types and catalytic levels or optimization of transesterification reaction temperature.

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