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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 9 Documents
Search results for , issue " Vol 32, No 1 (2015)" : 9 Documents clear
Molecular Analysis of Synedrela Nodiflora (L.) Gaertn. Resistance Against Fomesafen using RAPD Markers Dwiati, Murni; Susanto, Agus Hery
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.290

Abstract

Both morphological and protein studies reveal that differences are observed between resistant Synedrella nodiflora against fomesafen and its susceptible wildtype. These are, however, more or less influenced by environmental factors, so that molecular analysis employing DNA markers is necessarily required. The methods involved total genomic DNA extraction using modified CTAB protocol following Doyle & Doyle (1990), RAPD marker amplification and visualization of RAPD markers prior to data analysis. Ten RAPD markers were used, but only seven of them showed polymorphism.  Calculation of genetic distance and variation was carried out employing PopGen software.Based on the RAPD markers used in this study, it can be concluded that genetic distance between susceptible and resistant S. nodiflora is higher than that within susceptible samples supporting our previous morphological and protein data, although genetic variation among susceptible individuals seems to be significantly high.
Karakteristik Makro Algae Berzat Kapur di Perairan Tanjung Sira Lombok-Barat Kadi, Achmad
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.295

Abstract

Coastal waters of Tanjung Sira has calcareousalgae of the genus Halimeda limestone, Padina, Amphiroa, Galaxaura, Corallina, Hydrolithon, Mesophyllum, Peysonallia, Porolithon and Sporolithon. The substrate that used as habitat are  sand, coarse sand, rocks and dead coral rubble. Calcium carbonate contained on calcareous algae fungsioning as adhesive and encrusting dead coral, shells of mollusks that have decayed and massive objects in the waters of the sea. The research aims was to determine the growth characteristics of calcareousalgae in the reef flats, local distribution, calcium carbonate contain and its contribution as a frame work coastal reef waters. The research method using transect (Buckland et al., 1993). Identification of the type of aragonite and calcite according to Cordero (1977). Analysis of calcium carbonate according to Hillis (1980). The results showed that the green and brown calcareousalgae found in the reef flats, has thallus and tubers. Red calcareousalgae grew as encrusting on dead reefs and massif substrate. There are 16 species of calcareous algae that found in reseach area, 10 species containing aragonite mineral and 6 species containing calcite mineral. The content of calcium carbonate on each species obtained 100-450 g/m² consists of aragonite and calcite minerals. Calcareousalgae contribute in the new formation of  coral reef ecosystems. The other benefit of calcareaousalgae in the coastal waters is an additional food for herbivorous fish. The content of calcium carbonate on calcareousalgae species is used in pharmaceutical field as drug ingredients and supplements for humans.
Kajian Perubahan Bioekologi pada Restorasi Ekosistem Mangrove di Segara Anakan Cilacap Ardli, Erwin Riyanto; Widyastuti, Ani; Yani, Edy
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.291

Abstract

Mangroves are coastal ecosystems that have a very large role for humans and ecosystems in the vicinity. Mangrove condition in Indonesia, including in Segara Chicks Cilacap experiencing enormous pressure resulting in damage to the mangrove ecosystem. Mangrove restoration is the process of return of mangrove ecosystems of the conditions are broken into previously conditioned as well. The general objective of this study was 1) determine the conditions and amendments BioEkologi mangrove ecosystem restoration in the area of results Segara Chicks. Specific objectives in the study the first year is to determine: 1) the community structure of mangrove ecosystems (vegetation and fauna associations) at a restoration site in the region Segara Chicks, 2) the spatial variation community mangrove ecosystem in the area of restoration, and 3) the condition of the environmental factors that support the mangrove restoration in the region Segara Chicks. The method used was survey method with the technique of sampling cluster random sampling. The data obtained were analyzed multivatiate covering biodiversity analysis, cluster analysis, multi-dimensional sclae (MDS), and Bio-env using PRIMER-E program. The study shows the restoration of mangrove vegetation in the region have relatively low diversity (H <1), and in areas that have not been restored only dominated shrub species (Acanthus and Derris). Environmental factors generally favor mangrove Vegetasia life, and have the same tendency for each restoration location with a level of similarity> 95%.
Respon Pemberian Hormon 2,4-D dan BAP terhadap Pertumbuhan Subkultur Kalus Kedelai (Glycine max (L.) Merrill) secara In Vitro Ayuningrum, Kiki; Budisantoso, Iman; Kamsinah, Kamsinah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.296

Abstract

The purpose of this study was to evaluate the response of administration of a combination of 2,4-D and BAP on the growth of soybean callus subculture and determine the combination of 2,4-D and BAP most good for the growth of soybean callus subculture. The study design used completely randomized design (CRD) with a pattern factorials. The factor one e.i 2,4-D consists of 4 levels, namely: 0, 5, 10, and 15 ppm. A factor of 2 e.i BAP consists of 4 levels, namely: 0, 2, 4, dan 6 ppm. Every combination treatment repeated three times. Parameters measured include the percentage is growing callus, type of callus, dry weight and wet weight of soybean callus. The results showed that administration of the hormone 2,4-D and BAP can spur the growth of soybean callus subculture, the combination of BAP 2 ppm and 10 ppm of 2,4-D is the best combination for a percentage of callus and growing callus types, whereas the wet weight and the weight dried callus is not driven by a combination of the hormone 2,4-D and BAP
Penentuan Jenis Kelamin Benih Ikan Betutu (Oxyeleotris marmorata Blkr.) dengan Teknik Truss Morphometrics Atang, Atang; Suryaningsih, Suhestri; Abulias, Muh. Nadjmi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.292

Abstract

A study on The Gender Determination Of Betutu Germ (Oxyeleotris marmorata Blkr.) by Truss Morphometrics used survey method with Simple Random Sampling design, Betutu’s germ as sample were taken from Penjalin reservoir,  Brebes. The 26 distance of 13 pile points were based or Truss Morphometrics it were recorded as variables. The result of this research indicated that the size of Betutu’s A2 (origin anterior tip of snout at upper jaw – most posterior aspect of neurocranium), A4 (origin posterior point maxillary – origin of pelvic fin), B5 (origin of pelvic fin – origin of dorsal fin), B6 (origin of pelvic fin – origin of anal fin), D1 (insertion of anal fin – origin of lower caudal fin) and D3 (insertion of dorsal II fin – origin of lower caudal fin) can differentiate the gender of Betutu’s germ.
Transport Nutrien penyebab Eutrofikasi dari Daerah Tangkapan Air Waduk Mrica Banjarnegara Piranti, Agatha Sih; Soedarmadji, Soedarmadji; Waluyo, Gentur; Suwardi, Suwardi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.297

Abstract

The transport of nutrien causing eutrophication in water from catchment area especially fosforus (P) through runoff is determined by type of landuses, slop, soils type, and land cover (Jeje,2006) . The research was conducted using a survey method. The sampling technique was done by a purposive sampling on any flooding in the rivers that pass through areas with specific land use (settlement, forest, plantations, agricultural fields and rice field). The main parameters were river discharge and the content of nutrients causing eutrophication that was phosphorus (P) in rivers. The P loadingfrom cacthment area to Mrica reservoir was 11,526,706.14 kg/year and the load allocation (LA) was 3,833,587.70 kg/year. The reservoir operational of power generating dan flushing activity were able to manage the LA up to 1,147,329.09kg/year, so the TMDL of Mrica reservoir become 4,980,916.78kg/year. It was concluded that the highest transport of P was from paddy field and then followed by respectivelly agricultural (potato) field, plantation (perhutani) field, settlement area, and forest area.
Pengaruh Limbah Organik terhadap Status Tropik, Rasio N/P serta Kelimpahan Fitoplankton di Waduk Panglima Besar Soedirman Kabupaten Banjarnegara Widyastuti, Endang; Sukanto, Sukanto; Setyaningrum, Nuning
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.293

Abstract

The increased of organic substance pollutans in waters as one of stimulate factors for phytoplankton growth. The input of organic matter from antrophogenic  activity tend to influence the trophic state of reservoir. The aims of this research are to examine the influence of organic waste to Panglima Soedirman reservoir concerning the trophic state, Redfield ratio N and P, also the relation with abundance of phytoplankton. This research used survey methods with purposive sampling technique on 7 obsevation stations with 3 repeating conducted on May-July 2013. The result of the research showed that the input of organic matter increased in BOD5, COD and ammonia in reservoir and trophic state. Redfield ratio was shown 19,13-65,82 which means P as a limiting factor. Relationship between N/P with the abundance of phytoplankton with degree was strong correlated with chlorophyta and chrysophyta.
Keanekaragaman dan Kemerataan Spesies Anggota Ordo Anura di Lereng Selatan Gunung Merapi Tahun 2012 Yudha, Donan Satria; Yonathan, Yonathan; Eprilurahman, Rury; Indriawan, Septiana; Cahyaningrum, Eka
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.289

Abstract

Merapi volcano as one of national park is an ecotourism site which is very potential to visit. Information about species diversity for a national park is very important especially if its area fluctuated susceptibly. Two years after 2010 Merapi eruption, there are no research about species diversity and evenness of Anuran in that place. The research is carried out during June-November 2012 which is dry season. The objective of this research is to study the species diversity and evenness of Anuran in southern slope of Mount Merapi. The research is carried out in 6 locations; those are Kali Kuning, Telogo Muncar, Telogo Nirmolo, Petak Pitu, Bukit Turgo, and Bukit Plawangan. We used Visual Encounter Survey (VES) method combined with transect in Kali Kuning, Telogo Muncar, and Petak Pitu. VES method combined with time search in Telogo Nirmolo and Bukit Turgo. VES method combined with track exploration in Bukit Plawangan. Species diversity is analyzed with Shanon-Wiener diversity index. Species evenness is analyzed with Pielou evenness index. Total individuals each species is counted to know species abundance. Species diversity of anuran in southern slope of Mount Merapi is consisted of 12 species’ which is distributed in 6 locations. The highest diversity and evenness of anuran is at Bukit Turgo (H’=1.31; E=0.94). The lowest diversity and evenness of anuran is at Petak Pitu (H’=0.49; E=0.3). Species diversity of anuran in southern slope of Mount Merapi is low (Bukit Turgo and Kali Kuning) and very low (Bukit Plawangan, Telogo Muncar, Telogo Nirmolo, and Petak Pitu). Species evenness of anuran in southern slope of Mount Merapi is stable (Telogo Nirmolo and Bukit Turgo), still labil (Bukit Plawangan, Kali Kuning, and Telogo Muncar), and oppressed (Petak Pitu).
Variasi Morfologi Bambu Tali [Gigantochloa apus (Schult.F.) Kurz.] pada berbagai ketinggian tempat di Sub Daerah Aliran Sungai Pelus Hakim, Tria Fauzi Prabandani; Widodo, Pudji; Sudiana, Eming
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.294

Abstract

A study on the morphological variation of tali bamboo [Gigantochloa apus (Schult.f.) Kurz] has been conducted at various altitudes of Sub Watershed Pelus Banyumas, Central Java. This study was aimed at determining the morphological variations in relation to altitudes above sea levels. The method used was survey with stratified sampling.  The variables measured included culm diameter, culm internode length, culm sheath length and width, ovate-triangular blade length and width, leaf blade length and width, stem color and hairs, and altitude. The results of this study indicated that the altitudinal difference effect some morphological characteristics of tali bamboos such as: 1) green young seedling with black hairs found at 200-299 m and 300-399 m asl. 2) morphological variations are effected by altitude. Tali bamboo grows optimally at the altitude of 400 to 499 m asl. The results of regression analysis showed that the altitude has a relatively close correlation with somes of morphological character of tali bamboo.

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