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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 6 Documents
Search results for , issue " Vol 30, No 1 (2013)" : 6 Documents clear
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhyncos) Setelah Penyimpanan dalam Medium Berbeda Dikombinasi Krioprotektan Kuning Telur Berbagai Konsentrasi Atifah, Yusni; Saleh, Dadang Mulyadi; Pramono, Hendro; Sistina, Yulia
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.118

Abstract

The purpose of this study is to asses motility and viability of domestic duck (Anas platyrhyncos) spermatozoa after 6 day storing in preservation medium of different egg yolk cryoprotectant concentration in three different medium Tris, PBS or Ringer lactate. In this experiment, we applied 3 combination of factors as treatments : extender (Tris, PBS or Ringer lactate), egg yolk (EY) concentration (0, 5, 10, 15 or 25%) and storage time (0,1,2,3,4,5,6 days). Spermatozoa was obtained by massage technique, ejaculate then were swup up in the extender, the swup-up sperm then were incubated in preservation medium as each treatments subjected to motility and viability assesment before and after storage at refrigerator temperature (50C). The results showed that sperm motility and viability were highly significantly different (P<0.01) among 107 treatments. Extender factor as well as storage duration time were highly significantly different (P<0.01) affected the motility and viability of the treated sperm. EY concentration was significantly (P<0.05) affected viability, however there were no factor (P>0.05) affected motility of the sperm. The best treatment resulted in promising quality having the highest motility of 46,67 ± 32,15 5 motile was from the treatment of 10% EY in PBS stored for 4 days. The best viability result was from the tretament of 25% EY in PBS after 4 days storage resulted in 50,00 ± 36,05 % of viabie sperm. In conclusion this protocol could be applied for artificial insemination trial.
Kajian Etnobotani Mayarakat Adat Kampung Pulo di Kabupaten Garut Ramdianti, Nurlina; Hidayah, Hexa Apriliana; Widiawati, Yayu
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.125

Abstract

Ethnobotany is important in Indonesia because there are a lot of unknown things about the traditional plant usages by traditional people. One of them is the Kampung Pulo’s traditional people in Garut District of West Java’s province. This research aims to determine the diversity, usage and part of plants used by Kampung Pulo’s traditional people. The methods used are survey and data collection has been exploration, by interview and direct observation. The data were by analyzed by using descriptive analysis  plant diversity, usage for, part used and how they were used by Kampung Pulo’s traditional people. The result of the research showed that there were 93 plants species included into 42 families which is used to Kampung Pulo’s traditional people. The most species used includes Areca catechu L., Arenga pinnata (Wurmb) Merr., Cocos nucifera L., Carica papaya L., Sauropus androgynus (L.) Merr., Gigantochloa verticillata (Willd) Munro, Oryza sativa L., Curcuma domestica L., Kaempferia galanga L. and Zingiber Officinale Roscc.. Kampung Pulo’s traditional people use the plants in a wide variety way such as for foods, shelter, tools, drugs, cosmetics, rituals, dye and traditional ceremonies. Plant parts that often use continously are fruits, leaves, stems, flowers, tubers, saffrons and roots.
Keragaman dan Pemanfaatan Simplisia Nabati yang Diperdagangkan di Purwokerto Utami, Mei; Widiawati, Yayu; Hidayah, Hexa Apriliana
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.120

Abstract

A study to know the kinds of plant simplicia sold in Purwokerto and their usage as traditional medicine. A survey and sampling of simplicia plant has been conducted in Purwokerto.  An interview has also been done carried out for different respondants. The data recorded included simplicia type their local names, their pictures and classification, simplicia parts and their uses.  The result of this research showed that there are 50 types of simplicia included in 26 families used as traditional medicines in Purwokerto. The simplicia is used by boiling in a container made of clay soil, fried, poured by oil water, or cooked. Parts that are used as simplicia comprised are rhizome, leave, stem, bark, thallus, fruit, fruit peel, flower, herbals (root, stem and leaves) and seeds.  The uses of simplicia included carminative (remove stomach gas), anti inflammation, analgesic (pain healing), anti pyretic (heat lowering) cholagogum (making fluency of bile into duodenum), stomachic (increase appetite), anti spasmodic, choleritic (increase bile juice production), antelminthic (eradicate worm), sliming and tonic (body freshener).
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhynchos) Setelah Penyimpanan Refrigerator dalam Ekstender Dikombinasi Berbagai Konsentrasi Krioprotektan Gliserol Amelia, Arie; Saleh, Dadang Mulyadi; Pramono, Hendro; Sistina, Yulia
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.116

Abstract

The economic value of ducks has increased one Day Old Duck in large quantities and short time, effects on artificial insemination technologies improvement. Spermatozoa used for artificial insemination, could be derived from sperm preservation biotechnology, by means to maintain or extend their viability and motility of those spermatozoa. Extenders and cryoprotectant are needed for maintaining sperm motility and viability during storage or preservation. This study aims to assess motility and viability of local ducks spermatozoa after six days srotrage in 3 different extenders (Ringers lactate, Tris or PBS) in combination with 5 different concentrations (0, 2, 4, 6, and 8%) of cryoprotectant glycerol. Results showed that sperm motility and viability was very highly significant (P<0.01) different among treatments, extender factor as well as duration storage time also very highly significantly (P<0.01) affected motility and viability of treated sperm. However, glycerol concentration factor was not significant (P>0.05) affected motility and viability of the sperm. The best treatment, resulted in the highest percentage motility and viability of sperm with progressivity score >1, was the 8% glycerol in PBS storage for up to 2 days (75.00 ± 21.79% and 71.67±14.43%). This protocol could be applied in artificial insemination trial.
Aktivitas Protease dan Kadar Protein Tubuh Ikan Lele Dumbo (Clarias gariepinus) pada Kondisi Puasa dan Pemberian Pakan Kembali Hanum, Wieka Mahalida; Susilo, Untung; Priyanto, Slamet
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.121

Abstract

Three treatments and four replications in randomised completely design were conducted to reveal the protease activity and body protein content on catfish, Clarias gareipinus, under starvation and refeeding conditions. The result showed that the protease activity has no significant different (P>.05) in first week, but has significant different (P<.05) in second week after treatment.  Body protein content has significant different (P<.05) in first and second weeks after treatment. We concluded that the protease activity and body protein content decreased at deprivation, but increased after two weeks refeeding.
Aktivitas Amilase dan Laju Metabolisme Ikan Lele Dumbo (Clarias gariepinus) pada Kondisi Puasa dan Pemberian Pakan Kembali Pratiwi, Esti Dwi; Susilo, Untung; Priyanto, Slamet
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.123

Abstract

The study to determine amylase activity and metabolic rate of catfish, Clarias gariepinus under starvation and refeeding conditions has been conducted with three treatments and four replications in randomized completely design. The result showed that the amylase activity was significant different (P<.05) only at second week after treatment  and metabolic rate was significant different (P<.050 at first and second week after treatment. Conclutions, the amylase activity and metabolic rate of catfish have been decreased at starvation, especially at second week, but it recovery at refeeding condition.

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