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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 7 Documents
Search results for , issue " Vol 26, No 3 (2009)" : 7 Documents clear
Komunitas Biota Hewan Bentik Pada Danau Paparan Banjir Di Kalimantan Timur Lukman, Lukman; Chrismadha, T; Fakhrudin, Muhammad; Sudarso, J
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.3.164

Abstract

Lake Semayang and Melintang are floodplain lakes of River Mahakam, which are economically important as inland fisheries resources in Kutai Kartanegara Regency, East Kalimantan.  Benthic community in both lakes are still in rare exposed. Therefore, the aim of this research is to evaluate their community characteristics based on structure, diversity, and distribution pattern. The research was conducted in July 2006 at ten sampling stations of both lakes. Water quality in both lakes  in terms of temperature was between 28 and 32oC, while pH, turbidity and conductivity were low,  namely 3.74 – 5.39,  0.2 – 7.6 NTU, and 0.011 – 0.034 mS/cm, respectively. Dissolved oxygen was between 0.96 mg/L and 6.35 mg/L, and total organic matters (TOM) was high (26.8 – 57.5 mg/L).  Benthic community organism was arranged by mollusks, oligochaete and dipterans, consisting of 15 species with the abundance of 8 – 433 ind/m2. Aulodrilus piquet was the dominant species of oligochaete and Melanoides tuberculata was the dominant species from mollusks. The significant high number of A. piqueti was probably due to its preference on the habitat with the abundance of aquatic plants. Shannon Index Diversity of benthic organism community was low (<1.50), and it seemed to be related to the extreme condition of floodplain area environment. The distribution of benthic organisms did not show homogenous pattern which was also related to floodplain area condition.
Identifikasi dan Karakterisasi Isolat 23 Ducc Serta Kemampuannya dalam Memproduksi Enzim Inulinase Wijanarka, Wijanarka; Endang, K; Hermin, Hermin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.3.159

Abstract

Inulinase has a capacity to convert inulin into fructose hence it could be used in high fructose syrup production. Inulinase is not readily available in Indonesian market. This enzyme could be obtained from thermotolerant yeast found in Dahlia variabilis. Therefore this study was conducted to identify inulinolytic yeast in D. variabilis. The results showed  that an inulinolytic yeast isolate 23 DUCC from D. variabilis tubers was found. The inulinolitic yeast produces inulinase (E.C.3.2.1.7). Based on the identification and determination, the isolate is known to be Kluyveromyces marxianus.  The best optimation condition for this yeast was as follows: concentration of carbon source (inulin) was 0.75%, pH was 5.5 and time of fermentation was 53 hours. In such condition K. marxianus  produced inulinase up to 0.6481 IU.
Peningkatan Kualitas Akuakultur Menggunakan Teknologi Biofilter Mikroalga Imobil Riffiani, Rini
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.3.165

Abstract

Immobilized microalgae Chlorella pyrenoidosa was applied initially to nutrient and heavy metal removal of wastewater. Immobilized microalgae using alginate was then developed for aquaculture application, such as controlling fish water culture quality to uptake concentration of ammonium, nitrate and to increase oxygen level in water. During immobilization, algal cells maintained their respiratory and photosynthetic activities as that in the normal condition. The objective of this research was to examine the role of immobilized C. pyrenoidosa on controlling water quality by measuring ammonium, nitrate, and dissolved oxygen contents. Five aquariums consisting of 40 litres of water were filled with 20 Nila tilapia (Oreochromis niloticus) with the average weight of 1.6 to 1.7 g. The immobilized algae cells were packaged in two nillon porous bags (pore size of 2x3 mm in diameter) and each immobilized cell had 4 millimeter in diameter. Each aquarium was added with 3000, 4000, 5000, and 6000 immobilized cells. The treatment had two replications. The results showed that the aquarium filled with 4000 beads of immobilized cells gave the best yield.
Perancangan Primer Oligonukleotida untuk Polimerisasi in Vitro Gen Sukrosa Sintase Riupassa, Pieter Agustinus
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.3.182

Abstract

The most important problems in using polymerase chain reaction (PCR) are the efficiency of energy, cost and time due to gene amplification. Oligonucleotide primer design of sucrose synthase gene was conducted as a model of preliminary experiment to amplify gene using PCR. In plant cells, this gene plays an important role in carbohydrate metabolism, a sucrose molecule break down into glucose. This design involved some computer software as bioinformatics tools. Five data sequences of legumes were downloaded from gene bank using accession number of AF030231, AJ311496, X92378, X69773, and D10266 belongs to soybean, pea, alnus bean, fava bean, and mung bean, respectively. After sequences alignment, some conservative regions were determined as the basis to construct forward and reverse primer candidates. Furthermore, the candidates were tested for compatibility. The results showed that the oligonucleotide primers can amplify sucrose synthase gene with ± 1462 bp fragment size using 5’-AACTTTgTgCTTgA-3’ and 5’-TCCTTTgACTCCTTC-3’ for forward and reverse primer, respectively. Even the PCR process weren’t applied, those primers might be universal primers to amplify sucrose synthase gene of legume plants.
Isyarat Sentuhan Antena Semut (Dolichoderus Sp) Pada Tubuh Aphid (Aphis Sp) Untuk Memperoleh Embun Madu Sunjaya, Yayan; Buana, Harry; Halimah, Mimi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.3.183

Abstract

Ant, Dolichoderus sp, interact mutually with aphid (Aphis sp).  The ants specifically communicate with the aphid by means of antennal sign.  A hungry ant will approach an aphid and touch it dorsal part using ant antennae; in respond to this, will expel honey dew.  To further study the pattern of communication between the ant and the aphid, their movement was recorded using Handycam JVC GR-SXM 300 AC.  The records were processed using Adobe Premier Pro 1.5.  The results showed that the ant antennae moved in a specific pattern which could be classified into 4 main steps.     The first step consisted of 8 movements which formed 2 movement patterns.  The second step consisted of 3 main repeated movements.  The third step consisted of 3 main repeated movements.  The fourth step consisted of 6 main movements which were repeated several times before the final movement.  Such pattern of antennae movement was recognized by the aphid as tactile signal of ant identity.
The Dynamic Or Testicular Activity Of The Hard-Lipped Barb (Osteochilus Hasselti C.V.) Under Extended Photoperiod Wulandari, A; Soeminto, Soeminto; Wijayanti, Gratiana E
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.3.184

Abstract

Hormonal manipulation has been widely used in aquaculture to promote fish reproduction.  However some concern has been raised in accordance to it effect of human health as well as environmental issues.  Therefore alternative approaches in promoting fish reproduction need to be continuously studied.  Photoperiod manipulation offers a favorable alternative since it is one of the most crucial environmental cues necessary for fish reproduction.  An experimental study has been conducted to evaluate the effect of various photoperiods on testicular activities in the hard-lipped barb (Osteochilus hasselti C.V.).  Four groups of fish were reared under different photoperiod i.e. 14L:10D, 16L:8D, 18L:6D and the control group were reared under natural photoperiod for eight weeks period. Forth nightly three fish of each group were stripped to aspirate milt for measurement of milt volume and sperm concentration, they were decapitated for gonado-somatic index calculation and spermatogenic activities.  The results showed that the gonado somatic index (GSI) of the control group, 14L:10D and 18L:6D increased throughout the experimental period, meanwhile GSI of the fish reared under 16L:8D increased up to 4th week then decreased until the end of the study.  The fluctuation of the GSI values was followed by the fluctuation of milt volumes and sperm concentration.  This can be concluded that rising daily photoperiod up to 16 hours could improve reproduction in male hard-lipped barb.
Konstruksi Mutan Pseudomonas sp. untuk Meningkatkan Produksi Indole Acetic Acid (IAA) melalui Mutagenesis dengan Transposon Wahyudi, Aris Tri; Panjaitan, Mutiha; Rachmania, Nisa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.3.158

Abstract

Pseudomonas sp. is one of bacterial groups having ability to promote plant growth and health. Of a hundred successfully isolated Pseudomonas sp. from soybean rhyzosphere, 98 were found to produce indole acetic acid (IAA) ranging from 0.33 to 16.02 ppm. These isolates are bacilli, motile, Gram negative, and showing positive oxidase assay. One of them, i.e. Pseudomonas sp. CRB17, can promote plant growth by means of significant stimulation of primary root length and lateral root number. This isolate was then subject to mutagenesis using transposon Mini-Tn5Km1 to increase IAA production. Mutagenesis was done by conjugation between E coli S17-1 (l pir) carrying transposon mini-Tn5Km1 (donor) and Pseudomonas sp. (recipient), resulting in conjugation frequency of approximately 3.1 x 10-5 cell per recipient. The resulted CRB17 mutants were then tested for their ability to produce IAA, one of which showed an increment of IAA production up to 77.5%, while some others showed no significant change or even had a reduction to 55.3%.  Sequence analyisis of 16S rRNA gene of Pseudomonas sp. CRB17 indicated that it has a high homology with that of Pseudomonas plecoglossicida (identical value of 99%). The results recommends that mutagenesis using transposon can be applied to increase IAA production, especially in Pseudomonas sp. CRB17.

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