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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 6 Documents
Search results for , issue " Vol 26, No 2 (2009)" : 6 Documents clear
Pemanfaatan Limbah Padat (fly ash) untuk Mencegah Cemaran Mikrobiologis dan Kimiawi Sampah Kota pada Ekosistem Rawa Zulkifli, Hilda
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.113

Abstract

The research on the utilization of solid waste (fly ash) has been conducted to avoid the further effect of microbial and of the municipality solid waste in the wetland ecosystem. The purpose of this research is to utilize the fly ash, the solid waste of the PLTU industry, as protective layer (geosynthetic liners/GCLs) at waste disposal areas in order to avoid microbial and chemical pollution to the environment. During the research, ponds with 6x6x4 m in size was added with GCLs (layer of 30 sacs of fly ash 25 kg/sac at each 2 meters of pile waste) and a control pond. The result shows that fly ash with the composition of SiO2 (42,92 %) and AlO2  (29,45%) can be utilized as a protective layer (geosynthetic liners), because after 3 weeks it can minimize the contamination of bacteria (total decrease of coliform bacteria > 50%) to the environment, and also reduce some toxic compound, especially heavy metal such as Pb (30,1%) and Cu (43,54%), and the content of nitrate (32,1%). Hence fly ash has economic and ecological values as a protection layer at the solid waste disposal area to avoid the environmental contamination of bacteria’s and chemicals.
Status Kerentanan Aedes aegypti (Diptera: Culicidae) pada Tahun 2006-2007 terhadap Malation di Bandung, Jakarta, Surabaya, Palembang dan Palu Ahmad, Intan; Astari, Sita; Rahayu, Resti; Hariani, Nova
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.119

Abstract

Studies on the susceptibility of Aedes aegypti collected in 2006-2007 from five cities in Indonesia, i.e. Jakarta, Bandung, Surabaya, Palembang and Palu were tested by using the WHO standard test to determine their susceptibility status to Malathion. The results showed that despite the fact that Malathion has been used for >32 years, in general Ae. aegypti from all study sites, except from Palembang, were still susceptible to Malathion. This paper offers some possible factors that might have involved in delaying the onset of resistance to Malathion.
Kajian Status Resistensi Tungau Hama Brevipapalpus phoenicis dan Tungau Predator Ambleyseius deleoni sebagai Dasar Evaluasi Aplikasi Pestisida Pratiknyo, Hery; Basuki, Edi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.115

Abstract

The Increasing demand of tea product this decade causes the tea become prospective commodity. In other hand, free residual of pesticide in the tea culture could inhibit the rise of this demand. Therefore the evaluation of pesticide application policy in the field should be conducted, especially in term of the effect of the residual of pesticide on the resistance status of the mite. The research had been done using survey and experimental methods. The aim of the research was to decide the resistance status of the pest mite of tea leaf Brevipalpus phoenicis and it main predatory mite Ambleyseius deleoni on the application of three kind of pesticides (herbicide, fungicide and acaricide), and its effect on predator-prey feeding link. The result shows that both B. Phoenicis and A. deleoni are still resistant to herbicide (Round Up 486 AS) and fungicide (Kocide 77WP), but they are susceptible on acaricide (Kelthane 200EC). Also pesticide application has no effect significantly on predator-prey link (predation level). The conclusion is pest mite B. phoenicis is resistant on fungicide (Kocide 77WP) and Herbicide (Round Up 486 AS) but it is not resistance on Acaricide (Kelthane 200EC). Application of pesticides has no effect significantly on predator-prey link (predation level), so predatory mite A. deleoni is still effective in biological control for its prey.
Pengaruh Pemberian Sitokinin Terhadap Pertumbuhan Palea dan Lemma Padi Melaui Kultur In Vitro Kamsinah, Kamsinah; Hardiyati, Triani; Sugiyono, Sugiyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.117

Abstract

A research has been carried out with the aims to: 1. study the influence of both the kind and concentration of cytokinins on palea and lemma growth in in vitro culture; 2. study the influence of palea and lemma age towards external application of cytokinin in in vitro culture; and3. determine the best palea-lemma age, kind and concentration of cytokinin which resulted the best growth of palea and lemma in in vitro culture. The used experimental method was Completely Randomised Design (CRD) with factorial treatment pattern. The applied treatment consisted of three factors i.e. palea-lemma age (U): (55, 60, and 65 days after planting); kind of cytokinin (S): (BAP and Kinetin); and Cytokinin Concentration (K): (0, 5, 10, and 15 M) with 3 replications. The observed variables were the palea and lemma growth with the parameters were palea-lemma length and width. The results showed that the kind of cytokinin had influenced the growth of palea-lemma in in vitro culture, and kinetin has better influence on the palea-lemma growth. The age of the palea-lemma determined the responsiveness of the palea-lemma towards external application of cytokinin. Older palea-lemma showed less responsive than younger ones towards external application of plant growth regulators. Moreover, the treatment combination (U1S2K2) (10 µM Kinetin applied to 55-day-old rice palea-lemma) had the best effect on increasing the size of palea-lemma of IR 64 rice.
Pemakaian Sel Hela dalam Uji Sitotoksisitas Fraksi Ethanol Biji Mimba (Azadirachta indica) Djajanegara, Ira; Wahyudi, Priyo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.112

Abstract

Traditionally, the neem (Azadirachta indica) seeds have been used as bioinsecticide. It was also used and known for long time as a fungicide and anti bacterial agents. Pre-toxicity experiment using brine shrimp lethality test (BSLT) method indicated that the ethanol fraction of the neem seeds was toxic to the shrimp larvae with LC50 38.79 μg/ml. This experiment was done to further investigate the possible role of ethanol fraction of neem (A. indica) seeds as an anti cancer agent using HeLa cell lines. Experiment was initiated by extracting the seeds using n-hexane by maceration. The macerate was then macerated further using 70% ethanol as solvent. The ethanol fraction was used in cytotoxicity assay using (cervix cancer) HeLa cell lines. Cytotoxicity assay was done by using direct counting method. The amount of living cells were observed and counted, death percentage was determined and probit analysis used to determine the LC50 value. LC50 value for ethanol fraction from the seeds of neem (A. indica) was 10.95 μg/ml for 24 hours; 7.33 μg/ml for 48 hours and 4.37 μg/ml for 72 hours based on cytotoxicity analysis using HeLa cells. This experiment concluded that the neem (A. indica) seeds have cytotoxicity toward HeLa cell lines which confirmed the previous toxicity experiment using brine shrimp lethality test (BSLT) method. Overall, this experiment indicated that neem (A. indica) seeds have anticancer activity.
Floristics Composition of Tomong Forest at Sambas, West Kalimantan Hidayat, Syamsul; Sudarmono, Sudarmono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.110

Abstract

Tomong forest at Sambas regency is a restricted tropical forest along Sambas riverbank area. The forest have been threatened and decreased due to development of oil palm plantation and human settlement. The plant plant diversity and natural ecosystem in this area should be conserved soon. Sambas botanical garden establishment, which is a serious effort to conserve this forest. Plant Inventory has been conducted on July 15-20, 2008, as the first step to prepare establishing the botanic garden. The floristic composition is a basic important data for botanic garden development planning. The result of inventory shows that there were 153 species of 433 individual trees in the plots sampling with total size of 10 ha (3.3% of total area). These species occurred in three-habitat type on the forest area i.e. riparian, freshwater swamp and heat forest. Dipterocarpaceae is the most important family and as an emergent tree in Tomong forest, there were 17 species in 5 genera belongs to this family, with totally 60 individual trees particularly growing on the sites ≥ 50 m asl. Other important families are Leguminosae, Myrtaceae, Euphorbiaceae, and Sapotaceae.

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