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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 7 Documents
Search results for , issue " Vol 23, No 1 (2006)" : 7 Documents clear
Pengaruh Elisitasi dengan Verticillium dahliae Kleb dan Rhizoctonia Solani Kuhn terhadap kandungan Gosipol dalam kalus Gossypium hirsutum L pada beberapa tingkat Subkultur Rahayu, Suci; Esyanti, Rizkita Rachmi; Siregar, Arbayah H
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.140

Abstract

An experiment on gossypol content hasbeen conducted on callus derived from Gossypium hirsutum L. subcultures 5, 6, and 7 after elicitation with Verticillium dahliae Kleb and Rhizoctonia solani Kuhn. Callus was cultured on solid Linsmaier and Skoog  (LS)  medium  supplemented  with 10 –5 M NAA and 10 –6 M 2,4-D, and subcultured for 5, 6, and 7 times. Callus was then elicited with 40  μg dry weight /ml homogenate of fungi derived from V. dahliae and R. solani, and harvested 0, 2, 4, and 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively using High Performance Liquid Chromatography (HPLC). The maximum gossypol content elicited with R. solani was obtained on callus subculture 5, which was harvested on 4 days after elicitation, i.e. 177,995 ±  0,248 μg/g dry weight,  whilst that with V. dahliae  was obtained on subculture 5, harvested four days after elicitation, i.e.108,021± 0,507 μg/g dry weight. The gossypol content of control callus on subculture 5, 6, and 7 was 37,885 ± 0,779 μg/g dry weight  23,170 ±  0,003 μg/g dry weight, 12,284 ± 0,221 μg/g dry weight, respectively. The subculture level, elicitor type, and harvesting time gave significant effect on gossypol content of G. hirsutum callus culture.
Aktivitas Harian Monyet Hitam Sulawesi (Macaca nigra) di Cagar Alam Tangkoko-Batuangus, Sulawesi Utara Saroyo, Saroyo; Mansjoer, Sri Supraptini; Tarumingkeng, Rudy C; Solihin, Dedy Duryadi; Watanabe, Kunio
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.145

Abstract

This study was conducted on Rambo II Group at Tangkoko-Batuangus Natural Preservation, North Sulawesi, from January to February 2004. Data were collected using scan sampling with two minute interval. The results showed that the daily activities of the monkey group consisted of feeding and foraging (34.55%), moving (18.70%), resting (28.65%), and social behaviour (18.10%). It could be concluded that there was no difference between the daily activities of Rambo II Group and those of Rambo Group, the original group before being fragmented into Rambo I and Rambo II Groups.
Seleksi In Vitro Embrio Somatik Kacang Tanah pada Medium dengan Polietilen Glikol untuk Simulasi Kondisi Cekaman Kekeringan Rahayu, Enni Suwarsi; Ilyas, Satriyas; Sudarsono, Sudarsono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.141

Abstract

This study was aimed to evaluate the effectiveness of in vitro selection for identifying PEG insensitive somaclonal variant of peanut somatic embryos (SE). In one of the experiments,  evaluation on responses of four peanut cultivars against selective medium containing polyethylene glycol 6000 (PEG 6000) was conducted and sub-lethal concentration of PEG was determined. In the other experiment, in vitro selection on selective medium containing sub-lethal level of PEG was conducted to identify PEG insensitive SE of peanut. Secondary SE of peanut cv. Badak, Kelinci, Singa, and Zebra were cultured on liquid MS medium supplemented with 16 µM of picloram and 5%, 10%, 15%, or 20% of PEG 6000. Survival of explant, average number of proliferated SE/explant, and total number of proliferated SE after in vitro selection were recorded monthly, up to three months. Sub-lethal level of PEG was defined as one inhibiting more than 95% of the total number of proliferated SE. In vitro selection on medium supplemented with sub-lethal level of PEG 6000 was conducted on at least 4000-5000 SE of peanut cv. Kelinci and Singa. The PEG insensitive SE was identified after subsequent three months of in vitro selection. The results showed that supplementation of PEG 6000 on medium for induction of SE inhibited proliferation of peanut SE. Sub-lethal level was obtained at 15% concentration of PEG 6000. The frequencies of obtaining PEG insensitive SE of peanut cv. Kelinci was 8% to 10%  and for peanut cv. Singa was 10% to 12%. The R0 plants of peanut cv. Kelinci (62 R0 plants) and Singa (48 R0 plants) regenerated from PEG insensitive SE were obtained and grown in the glasshouse to produce R1 and R2 seeds.
Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries) Herdis, Herdis; Surachman, Maman; Rizal, Muhammad; Boediono, Arief; Yulnawati, Yulnawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.142

Abstract

This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations.  Cement was collected from three mature rams using an artificial vagina once a week.  After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively.  The extended-cement was stored at 5oC.  Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days.  The  results showed that addition of trehalose did not significantly improved  the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5oC for 4 days.  In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.
Variasi Sekuens Gen Mitokondrial Sitokrom C Oksidase I dari Siput Lola (Trochus niloticus) Nuryanto, Agus; Solihin, Dedy Duryadi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.143

Abstract

A study on sequence variation in the mitochondrial DNA of Trochus niloticus has been conducted. Polymerase chain reactions (PCR) technique was applied to amplify cytochrome c oxidase I gene of 533 bp and ABI 310 aotomatic DNA sequencer was used to sequence the PCR product.  This study was aimed to know the genetic variation of T. niloticus and the suitability of partial sequence of cytochrome c oxidase I gene as a molecular marker in the genetic study.  The results showed that from two T. niloticus specimens, 12 polymorphic sites were detected yielding 2 haplotypes. This indicated a highly genetic variation in thecytochrome c oxidase I (COI) gene of T niloticus.  In addition, cytochrome c oxidase I gene was proved to be a suitable marker for the genetics studies of various species of organisms.
Beberapa Aspek Pertumbuhan Lamun Enhalus acoroides (Linn. F) Royle di Pulau Barrang Lompo Makassar Supriadi, Supriadi; Soedharma, Dedi; Kaswadji, Richardus F
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.139

Abstract

A study has been conducted to investigate some aspects related to seagrass Enhalus acoroides growth in Barrang Lompo Island Makassar by using a modified tagging method.  Tagging was applied monthly to 20 seagrass stands for seven months with weekly observations. The results showed that Enhalus acoroides growth had a quadratic pattern and was strongly affected by exposed environmental condition, turbidity, nutrient, and temperature.
Pengaruh Alkalinitas terhadap kelangsungan Hidup dan Pertumbuhan Ikan Lalawak (Barbodes sp.) Yulfiperius, Yulfiperius; Toelihere, Mozes R.; Affandi, Ridwan; Sjafei, Djadja Subardja
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.144

Abstract

This experiment was conducted to determine the effect of alkalinity on the survival rate and daily growth rate of Barbodes sp. The fish were reared in 12 aquariums with four different levels of alkalinity, i.e. 48, 78, 108 and 138 ppm. Each aquarium was filled with 10 fish of 12.25 to 12.64 gram in average body weight. The treatments were applied for 75 days. During the experiment, the fish were given commercial diet (pellet) of 5% rate of biomass weight at the frequency of three times a day. The results showed that the survival rate for all treatments was 100%, and the alkalinity suited for daily growth rate of fish lalawak was 78 ppm of CaCO3.

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