cover
Filter by Year
Al-Kimia
ISSN : 23022736     EISSN : -
Al-Kimia is a journal of chemistry that published by Department of Chemistry, Faculty of Science and Technology UIN Alauddin Makassar. The journal is published twice a year on January-June and July-December. This journal encompasses original research articles in all aspects of chemistry and related area. The journal, which was firstly published in July 2013, is a forum for communication of research results, review the results of research, methodologies and new approaches in research related to chemistry in Indonesia.
Articles
112
Articles
Penentuan Sifat Fisikokimia Madu Hutan (Apis dorsata) Asal Daerah Maros, Pangkep dan Gowa Propinsi Sulawesi Selatan

,, Sjamsiah

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (676.587 KB)

Abstract

Abstract: The physicochemical properties of forest honey (Apis dorsata) become one of the supporting qualities of a honey. Good honey can be viewed from the quality of honey which refers to the Indonesian National Standard (SNI). Therefore, this research aims to determine the physicochemical properties of forest honey from the village of Bonto Manurung, Maros district, Tompobulu village, Pangkep district and Paranglompoa village, Gowa district in accordance with SNI. The parameters tested in this research were analysis of water content, ash content, acidity, viscosity, reducing sugar (glucose), Hydorxy Methyl Furfuraldehyde (HMF) and metal contamination tests for As, Pb and Cd on honey. The method used in this study is in accordance with SNI and the instruments used are UV-Vis and ICP-AES. The results showed that the best physicochemical properties of honey were from the village of Bonto Manurung, Gowa district which had a moisture content of 19.19 %b/b, ash content of 0.045 %b/b, viscosity of 14.82 poise , acidity is 42.43 mL N NaOH/kg, reducing sugar is 69 %b/b and HMF levels are 2.99 mg/kg. Then the Tompobulu village of Pangkep district has a water content of 22.29 %b/b, ash content of 0.35 %b/b, viscosity which is 6.71 poise, acidity of 83.06 mL N NaOH/kg, reducing sugar which is 68 5 %b/b and HMF levels are 2.84 mg/kg. Finally, Paranglompoa village in Gowa district has a water content of 24.92 %b/b, ash content of 0.52 %b/b, viscosity of 2.68 poise, acidity of 51.5 mL N NaOH/kg, reducing sugar 53.5 %b/b and HMF level is 3.175 mg/kg. As for the analysis of metallic impurities, all samples examined forest honey is not contaminated by metals arsenic (As), cadmium (Cd) and lead (Pb).

ANALISIS HIDROKSIAPATIT DARI TULANG IKAN TUNA (THUNNUS ALBACORES) DENGAN XRF, FTIR, dan XRD

Chadijah, St

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (776.464 KB)

Abstract

Hydroxyapatite (Hap) with molecular formula Ca5(PO4)3OH is calcium apatite bioceramics which can be found in human teeth and bones. This material is synthesized through a hydrothermal process with calcium precursors derived from fish bones yellow fin tuna (Thunnus albacores) and phosphate precursor NH4H2PO4 0.547 M. On research conducted long stirring time variation and its effect on rendemen and the resulting crystallites products. As for the variation of the long time of stirring used are 1, 2, 3, 4 and 5 hours. The optimum time of formation of hydroxyapatite obtained within 3 hours stirring from which content was 96.4156%. The material was analyzed by FTIR and XRD. Results showed their characterization by FTIR absorption carbonate groups (CO32-) at wave number 1400-2400 cm-1, a phosphate ion (PO43-) at wave number 500-1050 cm1- and hydroxyl ions (OH-) at wave number 3500 cm-1. It is from XRD analysis, that size and the shape of the crystal which was obtain within 2 hours of stirring are 41,9753 nm and hexagonal.

Synthesis of N-Benzenesulfonyl-p-Coumaramide from p-Coumaric Acid

Wela, Nasriadi Dali, Wela, Arniah Dali

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (779.045 KB)

Abstract

Abstrak: N-Benzenasulfonil-p-kumaramida telah disintesis dari asam p-kumarat. Penelitian ini bertujuan untuk mensintesis senyawa N-benzenasulfonil-p-kumaramida dari asam p-kumarat. Senyawa target N-benzenasulfonil-p-kumaramida diperoleh melalui dua tahap reaksi sintesis, yaitu tahap reaksi asilasi dan amidasi. Senyawa produk sintesis diidentifikasi dan dikarakterisasi dengan titik leleh, uji KLT, spektrometer FTIR, 1H-NMR, dan 13C-NMR. Hasil penelitian menunjukkan bahwa senyawa N-benzenasulfonil-p-kumaramida yang diperoleh berbentuk padatan putih (rendemen 78,57%) dengan titik leleh 145-147 oC dan KLT (SiO2, n-heksana : kloroform = 6 : 4 v/v, Rf = 0.45).

Deteksi Bakteri Patogen Salmonella typhi pada Sayuran yang dikonsumsi Mentah Menggunakan Metode nested Polymerase Chain Reaction

Idar, Idar

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1615.908 KB)

Abstract

Salmonella typhi (S. typhi) infection is a zoonotic infection and known as salmonellosis. In the human body, salmonellosis causes an increase in high body temperature or known as typhoid fever that cause high morbidity and mortality in developing countries, especially Indonesia. It was found that out of 22 million cases of typhoid fever, 200 thousand of them ended in death. S. typhi often contaminates food that was consumed raw or not perfectly cooked, for example meat, eggs, dairy products, fruits and vegetables. The conventional method for detecting these bacteria is culture method which time consuming and need BSL 2  facilities. PCR was one of DNA based detection method that could overcome the culture method weakness. In this study conducted detection of Salmonella bacteria in raw vegetables which are usually consumed as fresh by using nested PCR method. The detection procedures were sample preparation; bacterial DNA isolation; amplification by using two sets of primer, ST1-ST2 in first round PCR and ST3-ST4 in second round PCR; and the characterization by using agarose electrophoresis. The results indicated that two of nine raw vegetables, tomatos and cabbages have been contaminated with Salmonella. We conclude that nested PCR could detect Salmonella contamination in raw vegetables.

ANALISIS KOMPOSISI ASAM LEMAK DARI MIKROALGA LAUT Navicula salinicola

Ramdanawati, Liska

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (881.701 KB)

Abstract

Indonesia is an aquatic country with potential biodiversity. One of the potential biodiversity is microalgae. Microalgae potential to be developed as a functional food with the characteristics of its fatty acids. The fattiest acids found in diatoms are myristic acid, palmitic acid, DHA and EPA. One of marine microalgae contains a lot of fatty acids is diatom Navicula salinicola. This study aimed to analyze fatty acid composition of marine microalgae Navicula salinicola. Navicula salinicola was cultivated in Guillard medium with 2.5; 5; and 7.5 % of nitrogen composition and cultivated for 11 days. Dry biomass obtained from harvesting was used for the extraction. Extraction of lipid from microalgae used Bligh & Dyer method with 9,9%; 14.5%, and 17.5% of oil yield. The result of GC-FID analysis that was 6 types of fatty acids that identified are palmitic acid, myristic acid, pentadecanoic acid, stearic acid, palmitoleic acid, eicosapentanoic acid (EPA). The highest fatty acid was palmitic acid with fatty acids percentage were 64.04%; 65.03%; and 53.55% respectively.Keywords: Diatom, Fatty acid, Gas chromatography, Navicula salinicola

Preparation of Creatinine Sensor Material By Molecularly Imprinted Method Using MBAA and AMPSA as Precursors

Karmanto, Karmanto

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1111.048 KB)

Abstract

Synthesis and preparation of creatinine sensor material by molecularly imprinted method using N,N-Methylenebisacrylamide (MBAA) and 2-Acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) as precursors, on nylon membrane had been conducted. The precursor i.e. MBAA and AMPSA is used as cross-linker monomer, and functional monomer respectively, with the creatinine as template.  The performance of creatinine sensor material been studied by instrument response (resistance) pattern analysis to concentration of creatinine.  Result of FTIR spectra and SEM photograph, indicated the formation of molecularly imprinted polymer (MIP) on nylon membrane. Logarithmic pattern is appears as instrument response to concentration change of creatinine. Linear correlation of instrument response and creatinine concentration is observed at concentration ranges 1 to 5 ppm of creatinine. Comparative studies show that performance of nylon coated membrane is better than non-coated membrane. Linearity, limit detection, and limit quantification value of coated membrane is 0.9592; 1.2066 ppm; and 4.0221 ppm respectively. In other hand non-coated membrane give 0.8799; 2.1607 ppm and 7.2044 ppm respectively. Keywords: Creatinine sensor, Molecularly imprinted polymer. Abstrak Sintesis dan preparasi material sensor kreatinin dengan metode molecularly imprinted menggunakan prekursor N,N-Methylenebisacrylamida (MBAA) dan asam 2-Acrylamido-2-methyl-1-propanesulfonat (AMPSA) pada membran nylon telah berhasil dilakukan. Prekursor MBAA dan AMPSA masing-masing digunakan sebagai monomer cross-linker dan monomer fungsional dengan kreatinin sebagai template. Karakteristik kinerja material sensor kreatinin dikaji melalui analisis respon instrument terhadap perubahan konsentrasi kreatinin. Hasil analisis spektra FTIR dan fotografi SEM, menunjukan bahwa membran nilon telah terlapisi oleh material polimer MIP yang terbentuk dengan template molekul kreatinin.  Uji performa sensor menunjukkan bahwa respon sensor terhadap berbagai variasi konsentrasi sampel latih bersifat logaritmik dan memiliki rentang kerja (daerah linier) pada konsentrasi 0-5 ppm. Membran nylon MIP-kreatinin memiliki performa yang lebih baik daripada membran nylon tanpa modifikasi dengan nilai linieritas R2 sebesar 0,9592; limit deteksi 1,2066 ppm dan limit kuantifikasi 4,0221 ppm untuk membran nylon MIP kreatinin. Kata kunci: Sensor kreatinin, Molecularly imprinted polymer.

ANALISIS FLAVONOID TOTAL AKAR TABAR KEDAYAN (Aristolochia foveolata Merr)

Jubaidah, Siti Jubaidah

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (747.394 KB)

Abstract

The Tabar Kedayan (Aristolochia foveolata Merr) plant located in the Malinau district of East Kalimantan has considerable biological active prospects as antioxidant, antibacterial, antiamuba, antiinflammatory, antihepatotoxic and antiviral. One of the secondary metabolites in this plant is the flavonoids that can be used as antioxidants.The aim of this research is to analyze the chemical content and total flavonoid content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total flavonoid content using spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution.The results of this study obtained the highest total phenolic average on ethyl acetate fraction of 1,09%±0,03 then n-hexane fraction of 0,52%±0,05 and the smallest level of ethanol-water fraction of 0,40%±0,03. Keywords: Tabar Kedayan (Aristolochia foveolata Merr), flavonoid, spectrophotometry

Uric Acid Biosensor Based on Biofilm of L. plantarum using Screen-Printed Carbon Electrode Modified by Magnetite

Fatonah, Dian Siska Rahma, Saprudin, Deden, Iswantini, Dyah, Nurhidayat, Novik

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1190.615 KB)

Abstract

Biosensor based on biofilm of L. plantarum has been successfully done for determination of uric acid in human urine compared with colorimetric enzymatic produced relative error of less than 5%. L. plantarum has uricase activity to react with uric acid, to maintain the stability of bacteria forming themselves into biofilms. Magnetite is known to increase sensitivity of the biosensor. The combination of magnetite-polyethylene glycol (Fe3O4-PEG) was used to modify the surface of Screen-Printed Carbon Electrode modified (SPCE) and the resulting modified electrode (biofilm/Fe3O4/PEG/SPCE) displayed good electrocatalytic activity to the oxidation of UA. The composition of biofilms with optical density 1, magnetite 100 mgmL-1 and PEG 3% v / v were able to increase the current up to 48% in 4mM of UA. The biosensor with an optimum composition produced good linearity with a concentration range, limit of detection, limit of quantitation, sensitivity, and repeatability were found to be 0.1 - 4.3 mM, 70 µM,  234 µM, 25.392 µA mM-1, 2.38%, respectively. This biosensor stable up to 49 days of measurement with the remaining activity was 90.70% and selective for interference compounds such as salt, urea, glucose, ascorbic acid. This method has a good stability, sensitivity, and potential application in clinical analysis. Keyword: biofilm, biosensor, L. plantarum, magnetite, uric acid.

Penurunan Konsentrasi BOD5, COD dan Padatan Tersuspensi pada Air Limbah dengan Menggunakan Teknologi Lahan Basah Buatan (Constructed Wetland)

De Rozari, Philiphi, Ledoh, Sherlly M.F

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (761.959 KB)

Abstract

Constructed wetlands are a promising solution technology to effectively treat domestic wastewater in developing countries at low cost. This paper reports the findings of the effectiveness of sand planted with Thypa latifolia with variation of the length of outflow measured from the bottom of the media in removing BOD5, COD, and suspended solids. The experimental design consisted of 12 vertical flow (VF) mesocosms. There were 3 treatments and one control based on the with variation of the length of outflow measured from the bottom of the media (7, 14, and 21 cm).  During the five months, the mesocosms were loaded with syntethic wastewater. The influent had a 2-day hydraulic retention time. Samples were monitored for BOD5, COD and TSS. The results showed the concetration of BOD5, COD and TSS reduced significantly after the wastewater were treated with constructed wetland systems.  The trend showed that the constructed wetland systems planted with Thypa latifolia had a better performance in comparison with the control. There were no significant differences of BOD5 COD, and suspended solid outflow among  7, 14 and 21  of constructed wetland systems. This indicated that the length of outflow measured from the bottom of the media did not influence the performance of constructed wetland systems in removing BOD5, COD and TSS.

Total Phenolic and Flavonoid Content, and Antioxidant Capacity of Red Ginger (Zingiber officinale var. Rubrum) Ethanolic Extract and Fraction

Pratoko, Dwi Koko

Al-Kimia Vol 6, No 2 (2018)
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Original Source | Check in Google Scholar | Full PDF (840.13 KB)

Abstract

Determination of total phenolic, total flavonoid, antioxidant capacity from ethanolic extract and fractions of red ginger (Zingiber officinale var. Rubrum) has been done. Red ginger contain the highest oleoresins among other ginger varieties. Oleoresins are component that had the most responsibility for pharmacological effect of red ginger, one of them is as antioxidant. Phenolic and flavonoid are highly contribute to the antioxidant effect of red ginger. The aim of this research is to determine the antioxidant capacity of extract and fractions of red ginger, and to investigate the correlation between antioxidant capacity with both total phenolic and total flavonoid. Ethanolic extracts are fractinationed with n-hexane, chloroform, ethyl acetate, n-butanol, and methanol solvent. Antioxidant capacity, total phenolic, and total flavonoid of all the samples are measured. Highest antioxidant capacity, total phenolic, and total flavonoid obtained from ethyl acetate fraction with TEAC 2143,893 ± 0,890 µmol/g, GAE 229,878 ± 1,330 mg/g, and QE 46,564 ± 1,804 mg/g. One way ANOVA and post hoc analysis show significant result with p value <0,01. Pearson correlation shows high positive correlation.