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BIOPROSPEK
Published by Universitas Mulawarman
ISSN : 18297226     EISSN : 26155435     DOI : -
Bioprospek: Jurnal Ilmiah Biologi invites the researchers and practitioners in the field biology, zoology, botany, microbiology, environmental sciences, biotechnology, and applied science that related to biology and its prospect either research paper or reviews.
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Articles 46 Documents
Pengaruh Air Rebusan Biji Alpukat (Persea americana L.) Terhadap Kadar Gula Darah Mencit (Mus musculus Mill.) Diabetesi Yang Diberi Diet Beras Warna Wahyuni, Dwi; Sudiastuti, Sudiastuti; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v11i1.127

Abstract

This study was undertaken to investigate the  effect of  the seed avocado cooking water on lowering  blood sugar levels in aloxan-diabetic mouse  which has  diet  of  black or red rice. The seed avocado cooking water was administered orally and the blood glucose level was measured weekly for  3 week. The  research used a design random complete with 24  male then divided into 6 groups to be treated.The hypoglycaemic activity was evaluated by comparing with the initial blood glucoce level. In aloxan-diabetic mouse, the seed avocado cooking water and diet  black or red rice showed their influence to decrease blood sugar levels between 42.72% s.d. 51.06%.The variation of combine the composition diet  showed  no difference in the effect of a decrease in blood sugar levels
PENGARUH REBUSAN DAUN BINAHONG (Anredera cordifolia) TERHADAP KUALITAS SPERMATOZOA MENCIT (Mus musculus L.) DIABETES Meliyana, Yeni; Sudiastuti, Sudiastuti; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

This research aimed to evaluate the effect of decoction of the leaves binahong in improving of sperm quality of diabetic mice induced by alloxan. The quality of mice sperm including number of spermatozoa, motility and morphology of spermatozoa were determined after 30 days. Completed randomized design was use in this research, 30 mice were devided randomly into 5 groups (negative control, positive control, first treatment dose of 29 mg, 57 mg doses of treatment II and 114 mg doses of treatment III). The results of the quality of spermatozoa were presented as mean ± standar error (SE). Analysis of Variances (ANOVA) was used to determined statistical different among the group, followed by Duncan Multiple Range Test (DMRT) with significant level 95%. The results found that there was an increase in blood sugar levels followed by a decline in the number of spermatozoa, motility of spermatozoa (P>0,05) and the increase in abnormal morphology of spermatozoa (P<0,05) in all groups except the negative control after induced. After being given a dose of binahong decoction variation of the results showed that the number and motility of spermatozoa was highest in the group PIII that were given doses of 114 mg a mean number of spermatozoa (21016,00 ± 130,171) and motility of spermatozoa (34,33 ± 0,88). Meanwhile, the lowest morphological abnormal spermatozoa are group PIII with a mean of (79,33 ± 0,88). The conclusion of this study is a decoction of the leaves binahong is able to improve the quality of spermatozoa diabetic mice along with a decoction of the leaves binahong doses in increments.
Pengaruh Air Rebusan Tepung Cacing Tanah (Lumbricus rubellus) Terhadap Titer Antibodi, Jumlah Leukosit Dan Jenis Leukosit Mencit (Mus musculus L.) Yang Diinfeksi Salmonella enterica Serovar Typhi Patimah, Patimah; Kusumawati, Eko; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

Penelitian ini bertujuan untuk mengetahui apakah air rebusantepung cacing tanah berpengaruh terhadap titer antibodi serumdarah mencit, serta jumlah leukosit dan jenis leukosit mencityang diinfeksi bakteri S. Typhi. Penelitian ini bersifateksperimental laboratorik dengan Rancangan Acak Lengkap(RAL), yang terdiri dari 5 kelompok perlakuan dengan 5ulangan. Masing-masing kelompok terdiri dari 5 ekor mencit.Kelompok terdiri atas Perlakuan I diberi air rebusan tepungcacing tanah (TCT) dengan konsentrasi 8%; Perlakuan II diberiair rebusan TCT dengan konsentrasi 16%; Perlakuan III diberiair rebusan TCT dengan konsentrasi 32%; Perlakuan IV diberiair rebusan TCT dengan konsentrasi 64% dan kontrol tanpapemberian air rebusan TCT. Hasil penelitian titer antibodiserum darah mencit akut demam tifoid menunjukkan perubahanpositif demam tifoid menjadi negatif demam tifoid pada tiapkelompok mencit P1, P2, P3 dan P4 (air rebusan tepung cacingtanah 8, 16, 32 dan 64%), pada kelompok kontrol tanpa airrebusan tepung cacing tanah mencit tetap mengalami demamtifoid. Rerata pengukuran jumlah leukosit tertinggi terlihat padakelompok P4 dengan konsentrasi 64% yaitu 8,61/mm3 danterendah pada kelompok kontrol yaitu 3.18/mm3. Mencit dikelompok P1 (Pemberian air rebusan tepung cacing tanah 8%)yaitu 3,14/mm3. Mencit di kelompok P2, P3 dan P4 mengalamikenaikan jumlah leukosit. Data analisis statistik, uji One WayAnova didapatkan perbedaan yang signifikan pada jumlahleukosit antara tiap kelompok mencit (P<0,05). Berdasarkandata analisis statistik jenis leukosit yaitu Neutrofil Segmen,Neutrofil Batang, Eosinofil, Limfosit dan Monosit, pada ujinormalitas didapatkan data berdistribusi normal (P>0,05) danhasil uji homogenitas diperoleh data yang homogen (P>0,05),kemudian dilanjutkan pada uji One Way Anova didapatkantidak ada perbedaan yang signifikan pada jenis leukosit antaratiap kelompok mencit (P<0,05).
Analisis Kandungan Timbal (Pb) pada Udang Putih (Penaeus merguiensis) di Perairan Muara Badak Magfirah, Andi; Hariani, Nova; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v11i1.122

Abstract

Study on Analysis of Lead (Pb) Heavy Metal in White Shrimp (P. merguiensis) on the Muara Badak Estuary was conducted in May until July 2016. This aims to determine how much the concentration of lead (Pb) heavy metals in water, sediment and white shrimp (P. merguiensis) in the Muara Badak estuary. The method is using purposive sampling at 3 stations namely, near the pier and the residents, the transportation lines near of fishermen and the transit of oil tankers. During the research the temperatures ranging between 29.705 ° C 30.025 ° C shows the range was quite good for the aquatic biota growth, pH ranged from 7.135 to 7.425 which included in the category of normal pH, salinity ranged from 3.64 to 22.415 ‰ which showed freshwater, TDS ranged 13300-2320 mg/L which included in the range of high and exceed the quality standards set, and DO range from 7.16 to 7.61 which included in the category of normal. Levels of lead (Pb) heavy metals were measured using Atomic Absorption Spectrophotometry (AAS). The mean levels of lead (Pb) heavy metals in the water from Station 1 to Station 3 are 0.515, 0.273 and 0.216 mg/L which exceeds the quality standards specified by KMNLH no. 51 2004 was 0.008 mg/L, the average concentration of lead (Pb) heavy metals in sediment was 0.844, 0.758 and 0.835 mg/kg which is still safe because it is below the quality standard is 36 mg/kg and the average concentration of lead (Pb) heavy metals on white shrimp (P. merguiensis) is 0.534, 1.079 and 0.921 mg / kg which exceeded the quality standards set by BPOM RI in 2009 at 0.5 mg / kg.
MORTALITAS PREVALENSI DAN INTENSITAS TELUR CACING PARASIT PADA KUKU SISWA SEKOLAH DASAR DI SDN 007 KELURAHAN BUGIS DAN SDN 007 KELURAHAN SUNGAI PINANG LUAR KECAMATAN SAMARINDA KOTA Meilinda, Fanellya; Hariani, NOva; Sudiastuti2, Sudiastuti
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 13 No 1 (2018): Bioprospek: Jurnal Ilmiah Biologi Volume 13 Number 1 2018
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

Worm infections are one of the common diseases that spread around the world. Media transmission is divided into two groups, namely the group of worms STH (Soil Transmitted Helminths) and the group of worms Non-STH (Soil Transmitted Helminths). The parasite entry into the body is divided into three way hand to mouth, vector (Intermediate hospes), and hospes definitive. The aim of this research is to know the prevalence and intensity of parasitic worm eggs, the difference of the number and types of parasitic worm eggs on the students nails at Elementary School 007 in Bugis village and Elementary School in Sungai Pinang Luar village. This research method uses descriptive quantitative and sampling technique using purposive sampling method. The Data collection in the field conducted interviews and cutting student nail samples. For sample analysis techniques used two methods namely floating method and sedimentation method. The results showed that the type and number of the highest eggs were found on the students nails at Elementary School 007 in Bugis village and Elementary School 007 in Sungai Pinang Luar village, are the same type of egg Ascaris lumbricoides, while the fewest are Oxyuris vermicularis. Prevalence and intensity on students nail at Elementary School 007 in Bugis village is 100% and its intensity is 12,25 grains/ind., while on the student’s nail at Elementary School 007 in Sungai Pinang Luar village that dominates is 100% and its intensity is 20.8 grains/ind.
KAJIAN ETNOBOTANI OBAT TRADISIONAL DAN PEMANFAATANNYA OLEH MASYARAKAT SUKU KUTAI DI DESA JAMBUK, PENAWAI DAN MUARA KEDANG, KECAMATAN BONGAN, KABUPATEN KUTAI BARAT Putri, Fiorhentina; Hendra, Medi; Susanto, Dwi
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

This research aims to determine the types of medicinal plants used by the tribal communities of Kutai, herb medicine, the way to processing of medicinal plants and the environment unit of medicinal plants. The method used is descriptive methods, Participatory Ethnobotanical Appraisal technique, interviews and snow ball and also transect walk systematic sampling. Data analysis using Ms Excel 2013.Has been found as many as 113 kinds of medicinal plants, for which 109 species are from 53 families and 4 other species are not identified. Potion used as many 38 types of potions. Medicinal plants was processed with crushed/mashed 51 species; boiled 43 species; use directly 13 species; kneaded 12 species; marinated/brewed 5 species; made the statue 3 species, slice 2 species; dried, squeezed, shredded, torn and plaited each of them 1 species. The most environment unit of medicinal plants was found in the yard (73%), groves or simpung (11%), stall 5%, not obtained 4%, the curb 4% and in the riverside (3%).
Jenis Tumbuhan Sumber Nektar Lebah Apis dorsata Fabr. Dari Desa Bumi Harapan Dan Desa Bukit Raya Kecamatan Sepaku Kalimantan Timur Wibowo, Eko Putra; Syafrizal, Syafrizal; Susanto, Dwi
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v11i1.128

Abstract

This research aims to find out what kind of plants that become the source of nectar for Apis dorsata Fabr. in two different villages, namely Bumi Harapan village and Bukit Raya village, in Sepaku Region, East Borneo, and also to identify the pollen morphology of the nectar found in those plants that contained in honey. Based on the identification results of the nectar found from both villages there are significantly different number in variety of plants between these two samples. In Bumi Harapan, 27 families and 64 species of plants were found as the source of nectar, with 7 dominant families
VARIASI MORFOLOGI POLEN DALAM MADU LEBAH Apis dorsata Fabr. YANG BERASAL DARI DESA HULU KELAY DAN DESA NYAPA INDAH KECAMATAN KELAY KABUPATEN BERAU Mayasari, Fony; Stafrizal, Syafrizal; Susanto, Dwi
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

This research was conducted in November 2015 to March 2016 in Anatomy Laboratory and Plant Systematic. This study aims to determine the morphology of the pollen that contained in the liquid bees Apis dorsata Fabr. and to determine the type of plant food sources of honey bee Apis dorsata Fabr. Based on the results of research that has been conducted on a sample of honey bees Apis dorsata Fabr. from the villages of Hulu Kelay And Nyapa Indah, while the estimating pollen found as many as 22 Famillies and 29 species of plants. The honey samples from the Hulu Kelay’s has found 17 famillies and 23 species of plants. The dominant plant as feed for bees Apis dorsata Fabr. in the village of Hulu Kelay has found Family of Moraceae and species of Antiaris toxicaria totalling 98 pollen. Whereas in honey samples derived from village of Nyapa Indah has found 13 famillies and 15 species of plants. The dominant plant as feed for bees Apis dorsata Fabr. in the village of Nyapa Indah  has found Family of Verbenaceae dan species of Vitex sp. totalling 90 pollen. Based on the morphology of the pollen (shape, size, ornament and arperture) and pollen with unit of monad dominates both samples. The shape are varies from oblate, suboblate, oblate spheroidal, prolate spheroidal, subprolate and prolate. Category size from small to large ornament consisting of psilate, perforate and echinate. Aperture consists of inaperture, monocolpate, monoporate, triporate, tricolpate, tricolporate and pantoporate.
Penetapan Kadar Genistein Dalam Ekstrak Metanol BijiKedelai Glycine Max L. Merr. Varietas Grobogan MenggunakanMetode KLT Dan HPLC Aryani, Retno; Astuti, Pudji; Moeljopawiro, Soekarti; Nugroho, Laurentius Hartanto
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

Soybean (Glycine max L. Merr.) is one of the foodstuffs that are oftenconsumed most of Indonesian. Soy contains phytoestrogens, whichhave chemical structures resemble the hormone estrogen in the body,namely the isoflavones, especially genistein. Genistein is known notonly have various beneficial physiological effects but also act as anendocrine disruptor is because estrogen can play a role as well asantiestrogen activity that affects the metabolism of sex hormones andrelated activities biologi. The objective of this study was to know thequality of genistein in soy extracts Grobogan varieties that have beenpeeled the husk with Thin Layer Chromatography (TLC) and thequantity of soy extract genistein levels Grobogan varieties that havebeen peeled the husk with a method of High Performance LiquidChromatography (HPLC). The method used for extraction ofisoflavones genistein is maceration. Soybean seed samples which havebeen hulled begins with removal of non polar compounds byextraction using n-hexane solvent extraction followed macerationusing 80% methanol. The methanol extract obtained fractionated witha stationary phase of silica gel GF 254, the mobile phase toluene-ethylacetate-aseton- formic acid 20: 4: 2: 1 (v / v). To determine whether ornot a compound genistein compounds in the extract by using acomparison standard genistein further Rf sample extract comparedwith the price of a standard Rf. While the determination of genisteincompound quantitatively with HPLC method genistein standard wear≥ 98% at a concentration of 2, 4, 6, 8, 10 ppm. The identification ofgenistein using Thin Layer Chromatography (TLC), shows the stainsthat have Rf of about 0.43. Results genistein content by HPLC showedpeaks of the chromatograms that have a retention time of about 2,050minutes. HPLC analysis results in three replication showed levels ofgenistein in soya bean varieties Grobogan was 1 g samples of soyextracts that have been flayed husk there was an average of 0.6356 mggenistein. In the soybean seed Grobogan varieties contains isoflavonesgenistein. Levels of genistein in 1 g of sample soy extracts Groboganvarieties that have been flayed epidermis was an average of 0.6356 mggenistein.
Studi Jenis Serbuk Sari yang Terdapat pada Saluran Pernapasan Atas Penderita Ispa di RSUD Dr. Kanujoso Djatiwibowo Balikpapan Sabila, Nabila Dienna; Syafrizal, Syafrizal; Susanto, Dwi
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v11i1.123

Abstract

This research was conducted in May 2016 in RSUD dr. Kanujoso Djatiwibowo Balikpapan by using acetolysis method. This aims to determine the pollen morphology and determine the plant species that becomes the pollen source which triggers the ARI also determine the periodic calendar of otolaringology patients in RSUD dr. Kanujoso Djatiwibowo Balikpapan all the time. The pollen analysis results on a sample of ARI mucus patients in RSUD dr. Kanujoso Djatiwibowo Balikpapan, was found there are 4 plant species and 3 families. The plants which expected dominating as the pollen source that triggers ARI are from Cyperaceae family which is Scirpus lacustris, Gramineae family which are Oryza sativa and Saccharum spontaneum also Liliaceae family which is Lilium regale. Based on the pollen morphology (shape, size, ornament and aperture), pollens with monad unit dominate the sample. The pollen shape are various from prolate, oblate spheroidal, subprolate and oblate. Ornament type is psilate. Aperture consists of triporate, pantoporate and inaperture. Therefore, it can be stated that the species of pollen source that triggers ARI has pollen morphology which are simpler and small. Based on ARI patient data in RSUD dr. Kanujoso Djatiwibowo Balikpapan from 2013 to 2015 is known periodically appear at the end of the year to early next year and the ARI interference occurs at the beginning of the dry season (April - May).