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Medical Laboratory Technology Journal
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Core Subject : Health, Science,
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area. Please read these guidelines carefully. Authors who want to submit their manuscript to the editorial office of Medical Laboratory Technology Journal should obey the writing guidelines. If the manuscript submitted is not appropriate with the guidelines or written in a different format, it will BE REJECTED by the editors before further reviewed. The editors will only accept the manuscripts which meet the assigned format.
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Articles 55 Documents
PENGARUH EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella Sativa) TERHADAP EKSPRESI ET-1 AORTA PADA MENCIT MODEL PREEKLAMPSIA Hipni, Rubiati
Medical Laboratory Technology Journal Vol 2, No 1 (2016): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (784.53 KB) | DOI: 10.31964/mltj.v2i1.37

Abstract

Abstract: The objective of this research is to analyze the effects of ethanol extract of black cumin seeds (Nigella Sativa) to the expression of ET-1 Aorta given to the preeclampsia model mouse. The research design utilizes experimental and the kind of design used is posttest only control group design. Mice preeclampsia model. This research is divided into 6 groups, namely: Negative control, Positive control, Model + extract of black cumin seeds 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day. After conducting the surgery to the mouse, an examination is done to the expression of ET-1 Aorta by using Immunohistochemistry method. one way anova Were used as statistical analysis. There was an effect of treatment of giving the ethanol extract black cumin seeds of ET-1 Aorta to the preeclampsia model mouse (p
Perbandingan Penurunan Kadar Formalin pada Tahu yang Direbus dan Direndam Air Panas muntaha, akhmad; haitami, haitami; hayati, Nurul
Medical Laboratory Technology Journal Vol 1, No 2 (2015): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (755.806 KB) | DOI: 10.31964/mltj.v1i2.20

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Tofu is a food with high protein content and the moisture content reaches 85%, so that it knows can not last long. Manufacturers know still use formaldehyde as a preservative. Handling to reduce formaldehyde levels in the know are soaked in hot water and boiled in boiling water. The purpose of this study was to determine the ratio decreased levels of formaldehyde in the know are boiled and soaked in hot water out. This type of research is True Experiment with posttest study design Only Control Group Design. The population in this study is tofu containing formalin. Then examined by spectrophotometry of formaldehyde levels in most groups as a pretest sample, others are given treatment and formalin levels checked by spectrophotometry. Data were analyzed using the Mann-Whitney test. Results of this study the average levels of formaldehyde in the know before the treatment is 68.668 ppm. Decreased levels of formaldehyde in formalin know after boiling for 10 minutes was 64.77%. Decreased levels of formaldehyde in formalin know with the treatment of immersion in hot water for 10 minutes is 33.1%. Based on statistical tests that have been conducted, it was found a significant difference between the reduced levels of formaldehyde in formalin boiled out and the know formalin soaked in hot water with sig. 0,000. Boiling know formalin reduce levels of formaldehyde greater than soaking out in hot water.
Angka Kuman pada Beberapa Metode Pencucian Peralatan Makan Ananda, Brilian Rizky; Khairiyati, Laily
Medical Laboratory Technology Journal Vol 3, No 1 (2017): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (825.774 KB) | DOI: 10.31964/mltj.v3i1.153

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One of the food hygiene and sanitation efforts that must be implemented by the Nutrition Installation is to maintain the hygiene quality of tableware consider the equipment as a source of food contaminants. The purpose of this research was to know the effectiveness of washing the tableware method to decrease the bacterial number. The research method uses true experimental design. The research design used was posttest only control group design and sampling technique was simple random. Instruments in the research is a set of tools for experiment, sampling, and examination. The independent variable in this research is washing method on the tableware using method A, B, and C while the dependent variable is the number of a bacteria of tableware. The results showed that the number of tableware bacteria in Sambang Lihum Psychiatric Hospital exceeded of the standard/not qualified, except on washing method A which fulfilled the requirement that is under 100 colony/cm2 of tool surface. The conclusion of this research is there is a significant difference to the three washing methods which is shown with the p-value of 0,027. Need to do further research on the washing process by adding sterilization process
MRSA dan VRSA pada Paramedis RSUD Ratu Zalecha Martapura Dwiyanti, Ratih Dewi; Muhlisin, Akhmad; Muntaha, Akhmad
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (754.675 KB) | DOI: 10.31964/mltj.v1i1.5

Abstract

Abstact: Staphylococcus aureus strains isolated from hospital generally been resistant to many antimicrobial-resistant strains of Staphylococcus aureus to vancomycin was rarely reported. Strains of Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections that are multiresistant to antibiotics. This study aims to determine the presence of bacteria Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin resistant Staphylococcus aureus (VRSA) on paramedics in the treatment room and the surgical ICU care hospitals Zalecha Martapura queen. This study used a descriptive survey method. The population in this study were nurses who worked in the surgical and ICU care that numbered 38 people. Sampling technique accidental sampling with a sample of 27 people. The survey results revealed 27 positive samples of Staphylococcus aureus 14 people, there is a sensitivity test results of 7 samples were resistant to cefoxitin (MRSA 26%), 4 samples were resistant to oxacilin and 7 samples were resistant to vancomycin (VRSA 26%). Concluded there is MRSA and VRSA in hospital Queen zalecha Martapura, it is advisable to increase the cleanliness of individuals and the use of personal protective equipment to reduce the incidence of nosocomial infections in the scope of the hospital. Keywords: MRSA; VRSA; Paramedic Abstrak: Galur Staphylococcus aureus yang diisolasi dari rumah sakit umumnya telah resisten terhadap berbagai antimikroba, Galur Staphylococcus aureus yang resisten terhadap vankomisin masih jarang dilaporkan. Galur Methicillin-resistant Staphylococcus aureus (MRSA) merupakan penyebab utama infeksi nosokomial yang bersifat multiresisten terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui adanya bakteri Methicillin-resistant Staphylococcus aureus (MRSA) dan Vancomycin resistant Staphylococcus aureus (VRSA) pada paramedis di ruang perawatan bedah dan perawatan ICU RSUD Ratu Zalecha Martapura. Penelitian ini menggunakan metode survey deskriptif. Populasi pada penelitian ini adalah perawat yang bekerja di ruang perawatan bedah dan ruang ICU yang berjumlah 38 orang. Tehnik sampling secara accidental sampling dengan jumlah sampel 27 orang. Hasil penelitian diketahui dari 27 sampel yang positif Staphylococcus aureus 14 orang, hasil uji sensitivitas terdapat 7 sampel resisten terhadap cefoxitin (MRSA 26%), 4 sampel resisten terhadap oxacilin dan 7 sampel resisten terhadap vancomycin (VRSA 26%). Disimpulkan terdapat MRSA dan VRSA di Rumah sakit Ratu zalecha Martapura, disarankan untuk meningkatkan kebersihan individu dan penggunaan alat pelindung diri untuk mengurangi kejadian infeksi nosokomial di lingkup rumah sakit. Kata Kunci : MRSA; VRSA ; Paramedis
Kandungan Asam Oksalat Sayur Bayam Fitriani, Herlena; Nurlailah, Nurlailah; Rakhmina, Dinna
Medical Laboratory Technology Journal Vol 2, No 2 (2016): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (673.028 KB) | DOI: 10.31964/mltj.v2i2.95

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Spinach is one of vegetable that is often used as processed food by the people of Indonesia. Besides containing many nutrients, spinach also contains chemical compounds that are negative, that is oxalic acid. Oxalic acid and its salts are water soluble that can be harmful because these compounds are toxic. This study aimed to determine differences in levels of oxalic acid in spinach when the water is allowed to stand at room temperature. The type of research was pre-experiment with one group pretest-posttest design. Samples of spinach water were divided into 4 treatment and the level of oxalic acid was examined by using permanganometry titration method. Results of research on each treatment showed oxalic acid levels on 0 hour standing was 3753.2 mg/L, 2 hours standing was 3980.0 mg/L, 4 hours standing was 4066.5 mg/L, and the 6 hours standing was 4254.5 mg/L. Repeated ANOVA statistical test results stated there were significant differences in the levels of oxalic acid in spinach water between 0 hour standing and room temperature-standing with a significance value of p
Pengaruh Genjer (Limnocharis flava) terhadap Penurunan Biological Oxygen Demand (BOD) Limbah Industri Karet Thuraidah, Anny; Puspita, Erie Indra; Oktiyani, Neny
Medical Laboratory Technology Journal Vol 2, No 1 (2016): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.547 KB) | DOI: 10.31964/mltj.v2i1.28

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Abstract: The levels of Biological Oxygen Demand (BOD) in the waste can be reduced by utilizing water plants such as velvetleaf (Limnocharis flava) as phytoremediation. This study aims to determine the influence of the many clumps Limnocharis flava to decreased levels of effluent BOD rubber industry with a variety clump Limnocharis flava much as 1, 2, 3, 4, and 5 clumps and clumps Limnocharis flava know the number of the most influential in lowering levels of BOD. This type of research is true experimental design with one group pretest-posttest. The research material was Limnocharis flava. The result showed the levels of initial BOD rubber waste amounted to 299.5 mg / l, decreased levels of BOD of waste rubber after being treated with Limnocharis flava with a variety of clumps. Decreased levels of BOD rubber waste for each successive treatment ranging from 1, 2, 3, 4, and 5 clumps genjer ie 13.65%, 14, 52%, 15.6%, 20.94%, and 29.05%. Based on test results obtained linear regression for each treatment their effect on levels of BOD using genjer rubber waste. Treatment using Limnocharis flava much as 5 clump results BOD levels decrease the total of up to 29.05%. This value has not met the standard the maximum allowable levels of BOD. Need soaking clumps Limnocharis flava more stout and longer so that the rubber waste reduction in BOD levels in accordance with the quality standards required.
Logam Merkuri pada Masker Pemutih Wajah di Pasar Martapura Rakhmina, Dinna; Lisa, Lisa; Kartiko, Jasmadi Joko
Medical Laboratory Technology Journal Vol 3, No 2 (2017): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.601 KB) | DOI: 10.31964/mltj.v3i2.172

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Currently, many brands and types of skin whitening cosmetics can be found on the market. Not all products are safe for consumers. Illegal or non-licensed cosmetics from BPOM are suspect because unlisted cosmetics tend to contain harmful chemicals such as mercury (Hg). Mercury (Hg) was including harmful heavy metals. The use of mercury has been banned since 1998 through PERMENKES RI No. 445 of 1998, where mercury in small concentrations can be toxic. Negative effects of mercury such as allergies, skin irritation and permanent damage to the nervous system, kidney, brain and disrupt the development of the fetus, especially when used in high doses. The aim of this study was to know whether there is mercury content on bleach mask that sold in Martapura market in March 2017. This study used a descriptive method and the sample was taken by purposive sampling technique. There were 10 samples of face bleach mask that cost around Rp5.000,00 - Rp20.000,00. Based on the results of 10 samples taken at Martapura market, 3 samples contained mercury of face bleach mask. The conclusion of this study is that mercury still used as bleach on the facial in the Martapura market. Therefore, BPOM needs more supervision and intensive counseling about the risk of bleaching cosmetics containing mercury, so the consumer can be careful in choosing cosmetics.
Hubungan Asupan Serat, Kolesterol, Natrium dan Olahraga Dengan Kadar Kolesterol dan Hipertensi pada Lansia magdalena, magdalena
Medical Laboratory Technology Journal Vol 1, No 2 (2015): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.1 KB) | DOI: 10.31964/mltj.v1i2.16

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Hypercholesterolemia is an excess of cholesterol in the blood, can be factors for heart disease and stroke. The cause of hypertension is heredity, age, sex, obesity (overeating), lack of exercise, stress, excessive salt intake, another effect: smoking, alcohol consumption, taking drugs. The purpose of this study was to determine the relationship of fiber intake, cholesterol, sodium and exercise with cholesterol and hypertension in the elderly group S. Parman Banjarmasin. Type of research is descriptive analytic research using cross sectional design of the study, which was conducted in a group of elderly Regional Health Center S. Parman Banjarmasin. This research was conducted in January through June 2015 with a sample size of 50 people. The data obtained is the intake of cholesterol, sodium, exercise and cholesterol and hypertension. The statistical test used is Chi Square test (p
Analisis Kadar Siklamat pada Es Krim di Kota Banjarbaru Nurlailah, Nurlailah; Alma, Nurhayati Aslami; Oktiyani, Neni
Medical Laboratory Technology Journal Vol 3, No 1 (2017): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.806 KB) | DOI: 10.31964/mltj.v3i1.148

Abstract

Sweeteners are one of the components which are often added to foodstuffs. Synthetic sweeteners are widely circulated in the community is cyclamate. Consumption of cyclamate that exceeds the dose will lead to bladder cancer. It will also cause lung, liver, and lymph tumors. The purpose of this study was to determine the presence of cyclamate in ice cream that exceeded the required threshold. The type of this research is the descriptive survey. The sample of this research is ice cream that produced by household companies from all ice cream traders in North Banjarbaru with 11 ice cream sellers. The variables in this study were cyclamate content found in ice cream. The results showed that 11 samples of ice cream were examined, 9 samples containing cyclamate with the highest level of 7.37 g / kg as cyclamic acid. The conclusion of this study is ice cream containing cyclamate found as much as 82% (9 samples), while 18% (2 samples) others negative, from 9 samples containing cyclamate positive, 89% did not qualify the requirements of PERMENKES Number 208 in 1985 that exceeds 2 gr/Kg as cyclamate acid. More specific checks are required for the analysis of cyclamate levels by other methods such as High Performance Liquid Chromatography.
Ketepatan Hasil dan Variasi Waktu Pendidihan Pemeriksaan Zat Organik Haitami, Haitami; Rakhmina, Dinna; Fakhridani, Syahid
Medical Laboratory Technology Journal Vol 2, No 2 (2016): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.144 KB) | DOI: 10.31964/mltj.v2i2.117

Abstract

The principle of organic substances determination in water samples is oxidized by an excess amount of KMnO4, then it is reduced excess amount of oxalic acid, the excess of oxalic acid is titrated back with KMnO4. Based on the procedures in ISO 06-6989.22-2004, a length of boiling time for organic substances determination in water samples is for 10 minutes. This study aimed to determine the effect of boiling time length to the accuracy of the organic substances determination result in water samples. This study was true experiment study with comparative study design. Samples were organic substances, specifically oxalic acid in distilled water in a concentration of 54 mg/L, which was examined for the organic substances level with a boiling time of 5, 10 and 15 minutes. The result on each boiling time length consecutively were 52,8 mg/L; 54,5 mg/L; dan 55,1 mg/L. The conclusion of the study was there was effect of boiling time length to the accuracy of organic substances determination result in water samples with the significance value of 0.002 (