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Medical Laboratory Technology Journal
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area. Please read these guidelines carefully. Authors who want to submit their manuscript to the editorial office of Medical Laboratory Technology Journal should obey the writing guidelines. If the manuscript submitted is not appropriate with the guidelines or written in a different format, it will BE REJECTED by the editors before further reviewed. The editors will only accept the manuscripts which meet the assigned format.
Articles
55
Articles
Red Pomegranate Extracts on Catalase Levels in Huvecs Culture Which are Exposed Preeclampsia Plasma

Januarsih, Januarsih

Medical Laboratory Technology Journal Vol 4, No 2 (2018): period Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

In a normal pregnancy, there is an increase in the production of free radicals, compared to on not pregnant and when the preeclampsia occurs the production is even more. This situation controlled with anti-oxidants. In general, antioxidants divided into two groups, namely: enzymatic antioxidants / primary antioxidants / antioxidant deterrents and nonenzymatic antioxidants consist of SOD, GPx and CAT. The purpose of this study is to determine the effect of giving Pomegranate Fruit extract to CAT levels in HUVECs who exposed to plasma preeclampsia. This study used a laboratory exploration method with samples: 1 negative control group (HUVECs culture exposed to normal 2% plasma pregnancy). One positive control group (HUVECs culture exposed to 2% preeclamptic plasma) and three treatment groups (HUVECs culture exposed to plasma 2% preeclampsia was given red pomegranate extract at different doses of 14 ppm, 28 ppm, 56 ppm, followed by calculating the amount of CAT which was a marker of intracellular antioxidants. Using the assumption of normality and homogeneity of variance tests that was using the Levene test as a prerequisite for parametric statistical testing, (2) One Way ANOVA Test (F Test), and (3) Pearson correlation test. The result is Red Pomegranate extract can prevent a decrease in CAT levels at a dose of 56 ppm. In conclusion, the Pomegranate Fruit extract can increase CAT levels

The Reduction of Carbohydrate, Fat and The Increment of Protein Content of Some Nigerian Diets by Traditional Fermentation

Elijah, Ehoche E, Adeyemi, Henry Y

Medical Laboratory Technology Journal Vol 4, No 2 (2018): period Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Fermentation is vital to African food processing. Its effects on the percentage carbohydrate, proteins, lipid and moisture composition of laboratory prototypes of the fermented seed from Parkia biglobosa, (Dawadawa) condiment paste, fermented milk (Nono), corn (Zea mays)-based pap (Akamu), soybean (Glycine max) based-cheese paste (wara) and soy-milk (soymilk). The major macro-nutrient and moisture contents of each food product and their respective substrates were determined using standard methods and compared. The result showed that there was a noticeable fall in the carbohydrate content in the Corn (56.23±9.09 %) as it was converted to Akamu (7 .63±2.67 %) just as was noticed in the fermentation of Nono (11.99±2.67 %) from fresh cow milk (42.3±1.60 %). The similar trend was also found in the fermentation of the lipid-containing soy bean seed (41±7) to soy wara (7.6±2 %) and soymilk (5.6±2.2 %). However, there was an increase in the protein content from the fermentation of Parkia biglobosa seed: 31.62±0.83 - 34.17±3.6 % in Dawadawa and 25.25±0.59 - 37.74±1.8 % in Nono. Moisture contents of the various fermented food products also increased as follows: from 9.00 ±0.01-90.0±0.70 in Akamu; 89.0±0.58 into 92.7±0.98 in Nono, 13.0±0.87 -33±0.01 in Dawadawa paste, and 5.0±0.01 - 39±1.41 % in soy milk and 31 ±1.4 % in soy wara. These show that fermenting foods could reduce their carbohydrate and fat content relatively but increase their protein content. These cannot be overemphasized considering the problem of malnutrition which is prevalent around this part of the world.

Colposcopy results in Smear negative, High-risk HPV positive patients

Keskin, Deha Denizhan

Medical Laboratory Technology Journal Vol 4, No 2 (2018): period Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Cervix cancer is an HPV (Human papillomavirus) related cancer, and HPV positivity is necessary even if there is no cytology abnormality. We aimed to determine the ratios of 13 high-risk HPV types in cases with high-risk HPV positivity without cervical smear pathology referred to our clinic and to determine the relation of HPV types with age, parity, menopausal status, and abnormal histopathological results. Two hundred forty-one cases included in the study, which referred to us because of HPV positivity and colposcopically biopsied between January 2014 to January 2018. HPV prevalences were investigated. The relationship between HPV types and variables such as age, parity, menopausal status examined. The mean age of 241 patients included in the study was 46,1+8,8. The parity average was 2,4+1,1. Sixty-five of the patients (27%) were postmenopausal. Of the 241 HPV-positive patients, 172 (71,4%) had only high-risk HPV viruses. The frequency ranking of HPV types was as follow; 16, 31, 51, 56, 18, 52, 35, 58, 39, 68, 45, 33 and 59. According to the HPV types, the average ages were as follow; 18 (43,6 years), 33 (40,1 years) and 51 (41,9 years) were younger than the average age. 35 (48,7 years), 39 (48,5 years), 52 (49,1 years) and 68 (51,3 years) were older than the average age. 16 (44,9 years), 31 (47,9 years), 45 (44,3 years), 56 (47,3 years), 58 (46,9 years) and 59 (46,7 years) was similar the average age. There was no significant difference between the parities according to HPV types (2 to 2,7). According to the HPV types, the menopausal state was as follows; 39 (50%), 56 (50%) and 68 (53,8%) mostly observed in the postmenopausal period; A small proportion of 33 cases (12,5%) was postmenopausal. The rate of severe dysplasia according to colposcopic biopsy related with HPV types was; 58 (40%), 56 (30,8%), 18 (28%), 45 (27,3%), 31 (26,1%), 39 (25%), 59 (16,7%), 35 (14,3%), 51 (13,8%), 33 (12,5%), 16 (11,8%), 52 (8,3%). The prevalence of HPV types, the age at which they saw, the menopausal status and the potential for the formation of severe dysplasia are highly variable. We think that routine screening programme, colposcopy indications and vaccination program should cover all HPV types according to data.

Evaluation of the i-STAT Blood Gas Analysis System in Cardiovascular Surgery

Kantekin, Çiğdem Unal, Ercan, Müjgan, Oğuz, Esra Firat, Demirdaş, Ertan, Atılgan, Kıvanç, Sipahi, Mesut, Çiçekçioğlu, Ferit

Medical Laboratory Technology Journal Vol 4, No 2 (2018): period Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

The aim of this study was toinvestigate the compatibility of the parameters measured with the i-STAT blood gas analyser and the conventional blood gas analyser Rapid Point 500 (Siemens Healthcare Diagnostics, USA) in patients who underwent cardiovascular surgery. This clinical study included fifty patients undergoing coronary artery bypass surgery. Fifty whole blood samples were portioned and measured on the i-STAT and RP500 laboratory analyzers. The compatibility between pH, pCO2, pO2, Hb, Na+, K+, iCa2+ and glucose values was investigated.There was a good correlation of the i-STAT analyser with the RP500 analyser, with the exception Hb and Na+. Also all parameters except for Hb and ionized calcium were found to be within acceptable range in terms of clinical decision limits. It is very important that the point-of-care devices give accurate results as well as quick results. For this reason, we absolutely think that the point of care devices should be subjected to external and internal quality control programs, users should be trained regularly and feedback studies should be done.

Comparison Analysis of Total Cholesterol Level Examination Between Photometry and 3 Parameters Point of Care Testing Device

Nursidika, Perdina, Mahargyani, Wikan, Anggraeni, Fitri Kurnia

Medical Laboratory Technology Journal Vol 4, No 2 (2018): period Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Total cholesterol is the composition of many substances including cholesterol, triglycerides, LDL cholesterol, and HDL cholesterol. Cholesterol examination is one of the most frequent tests required in the laboratory to monitor vascular and cardiovascular diseases. Most clinical pathology laboratories use photometer to perform clinical chemistry checks. Cholesterol testing can also be done with Point of Care Testing (POCT) which has a working principle of biosensor technology. This research method is experimental, using 40 samples that can represent normal and pathological levels. All samples will be checked for total cholesterol with a photometer of CHOD-PAP method and 3 POCT Lipid Pro. The results showed linear regression y = 0.955x + 1.8325 with R2 of 0.9955. The linear regression value is calculated by Total Error (TE), while the Total Error Allowable (TEa) cholesterol is 10%. The bias value is 0.31%, TE for normal level = 5.92% and TE for high pathological level = 3.00%, it can be stated the result of examination can be compared or accepted. The% TE value obtained is less than the TEa value of cholesterol. It can be concluded that the total cholesterol results examined by the photometer and LipidPro are comparable. For further research it is advisable to use a total cholesterol sample that has a value of more than 400 mg/dL.

Study of Culture and Sensitivity Pattern In Urinary Tract Infections in A Tertiary Care Center in Nepal

Chapagain, Binod, Bhandari, Parshal, Aryal, Binod

Medical Laboratory Technology Journal Vol 4, No 2 (2018): period Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

The main purpose of this study was to find out the causative agents of urinary tract infections (UTI) and their culture and antibiotic sensitivity in patients visiting Tribhuvan University and Teaching Hospital (TUTH). A retrospective study conducted among 155 patients, aged from 25-50 years with culture-positive UTI, who visited TUTH from 1st April 2017 to 30th September 2017. A culture of midstream urine was done to find out causative agents and their antibiotic sensitivity performed. Data were evaluated using Microsoft Excel 2016. Female were more affected than males. Escherichia coli (E. coli) was the most common microbes causing UTI in 53% patients. Most of the isolates on culture were Multi-Drug Resistant (MDR) strains to comprise 52%. Of the total gram-negative organisms, 33.9% were Extended Spectrum β-lactamase (ESBL) producers, and 3.57% were Metallo β-lactamase (MBL) producers. 29.41% of Staphylococcus were resistant to methicillin. E.coli is the most common organism causing UTI among adults. Multidrug-resistant has appeared alarming with resistant to most of the first line antibiotics.

Potention of Active Charcoal from Musa Paradisiaca and Manihot Utilissima Shell in Degrading River Contamination

Nurlailah, Nurlailah, Thuraidah, Anny, Kustiningsih, Yayuk

Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

River water is one of type water surface which is a lot of finding in South Kalimantan. Generally, this water used by the citizen for cooking and bathing. But along with era growth, this river becomes as disposal of various industrial waste. The contamination of heavy metal like Fe, Mn, Pb, Cd, and turbidity enhance progressively and degrade the water quality. Some natural substance which can be used to improve river water quality is active charcoal from Musa paradisiaca and Manihot utilissima shell which is easy to get. Target research is determined to find optimum dose of active charcoal from Musa paradisiaca and Manihot utilissima shell that capable to degrade heavy metal contamination and turbidity in water river. It used experimentally with pretest and post-test with control group design. A result of research showed the optimum dose of active charcoal Musa paradisiaca shell was 15 gram , it could absorb Pb 28,8% and Mn 24,6%, but 10 gram can degrade turbidity until 83,8 %, while the optimum dose of Manihot utilissima shell to 25,4% Pb and degraded 77,5% of turbidity was 15 gram. Statistical test result with Kruskal Wallis got the p-value less than 0,05 it meant there was a difference between the treatment of active charcoal from Musa paradisiaca and Manihot utilissima shell in degrading the contamination. A conclusion is those active charcoal having a potential to enhance the water river quality. Suggested to use another activator to the potential like stirring, time of contact and the different mass.

Lime Soap as Bacteria Reducer

Dwiyanti, Ratih Dewi, Lutpiatina, Leka

Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).

Blood Glucose Level And Candida spp. Growth In Elderly Diabetes Melitus Patients

Rifqoh, Rifqoh, Aslamiah, Syaidatul, Cahyono, Jujuk Anton, Roebiakto, Erpan

Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Diabetes mellitus is a presdisposing factor against infection, especially in orofacial area. Infectious diseases are more frequent serious in patients with diabetes mellitus, which potentially increases their morbimortality. The more frequent oral mucosa infection of DM patients is candidiasis which caused by Candida spp. This research is aimed to obtain the correlation between fasting blood glucose level and Candida spp. growth in elderly diabetes mellitus patients. This analytical survey research used cross-sectional design through GOD-PAP methode for fasting blood glucose level test and macroscopic method for total colony of Candida spp. growth. The result of fasting blood glucose level test average 186,06 mg/dl the highest level is 492,90 mg/dl and the lowest one is 72,08 mg/dl. Meanwhile, the result of Candida spp. is 131 at the average and the highest growth is 350 and the lowest one is 12 colonies. The result of the research indicates that there is a rising of Candida spp. to level of fasting blood glucose in elderly diabetes mellitus patients. Based on correlational test of spearman, it gains significant value 0,001 < ?± = 0,005 of that shows there is a correlation between fasting blood glucose level and Candida spp. growth in diabetes mellitus elderly patients with the strenght relation (r) = 0,572 which means in medium level. Further researches about the influence to the other presdisposing factors of Candida spp. growth are recommended

Correlation Between Onset of Diabetes Mellitus and Nitric Oxide Levels in Patient with Type 2 Diabetes Mellitus

Orno, Theosobia Grace, Arif, Mansyur, Idris, Irfan

Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

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Abstract

Type 2 diabetes mellitus (DM) is associated with increased risk of endothelial dysfunction if it lasts a long time without control. This study aims to connect the Onset of Diabetes Mellitus (DM) with Nitric Oxide levels in patients of type 2 diabetes mellitus. The study used cross-sectional study method. The samples were 86 subjects, consisting of 38 subjects of Type 2 DM controlled and 48 subjects of Type 2 DM uncontrolled. The results of the Kruskal-Wallis statistical test showed no significant difference between the Onset of DM and Nitric Oxide levels in the categories of 4-6 years (19.4 ?± 10.1), 7-9 years (17.3 ?± 9.3) and 10-12 years (13.3 ?± 8.5) (p=0.06). Furthermore, the Spearman correlation test revealed a negative correlation between the Onset of DM and Nitric Oxide level in patients with Type 2 DM with and without control (r =-0.217). The level of Nitric Oxide (NO) can consider as a predictor of long-term complication in patients with type 2 DM.