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e-Journal BUDIDAYA PERAIRAN
ISSN : 23373768     EISSN : -     DOI : -
Core Subject : Social,
e-Journal BUDIDAYA PERAIRAN merupakan jurnal ilmiah yang mempublikasikan hasil-hasil penelitian maupun ulasan (review) dibidang budidaya perairan baik budidaya air tawar, payau mapun laut. Artikel jurnal dapat ditulis dalam bahasa inggris atau bahasa Indonesia. Jurnal diterbitkan 3 kali setahun yaitu bulan Januari, Mei dan September
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Articles 5 Documents
Search results for , issue " Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013" : 5 Documents clear
Evaluation of The Use of Garlic (Allium sativum) in Enhancing Nonspecific Immune Response and Growth of Nile Tilapia (Oreochromis niloticus) Marentek, Gercyana; Manoppo, Henky; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
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Abstract

The objective of this research was to examine the effect of garlic (Allium sativum) at different doses on nonspecific immune response and growth of nile tilapia (Oreochromis niloticus). Experimental fish was nile tilapia measuring 8-15 cm with an average weight of 10.4 g per individual. Before used in the experiment, fish were reared in fiber tank for two weeks for acclimatization. After acclimatization, the fish were moved into glass aquarium (50 cm x 30 cm x 30 cm) at the density of 15 fish/aquarium. Each aquarium was equipped with aerator. Five doses of garlic as treatments used in this research consisted of A= 0 g/kg diet, B= 10 g/kg, C= 20 g/kg, D= 30 g/kg and E= 40 g/kg. Fish was fed experimental diet at a dose of 5% of body weight per day, twice a day at 08.00 and 17.00. Data collected at the end of the experiment included immune parameters (TLC and phagocytosis activity) and growth of fish. ANOVA was used to evaluate the effect of treatments on immune response and growth of fish while Duncan Test was used to evaluate the different effect between treatments. Research result showed that after four weeks of feeding, TLC of fish fed diet supplemented with garlic significantly different (p<0,01) as compared to that of control fish. The highest number of TLC was observed at treatment C (15.413x107 cell.ml-1) followed by treatment B (15.39x107 cell.ml-1), D (13,920 x 107 cell.ml-1), E (13,582 x 107 cell.ml-1) and A (12,195 x 107 cell.ml-1). TLC of fish in treatment C was different significantly compared to control as well as to other treatments. Phagocytosis activity of fish in treatment C also increased significantly (p<0,01) compared to control. The highest PA was achieved in treatment C and then treatment B, D, E and A. Growth of fish in treatment C was different significantly compared to control fish as well as other treatments. As conclusion, oral administration of garlic at 20 g/kg diet could enhance nonspecific immune response and growth of nile tilapia. Key words: Garlic, Immune response, Total leukocyte count , Phagocytosis activity, Growth
Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
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Abstract

This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus
Teknik Budidaya Lobster (Cherax quadricarinatus) Air Tawar di Balai Budidaya Air Tawar (BBAT) Tatelu Lengka, Kedis; Kolopita, Magdalena
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
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Abstract

The aimed of this field practice was to study the hatchery technique of freshwater lobster at Board of Freshwater Aquaculture, Tatelu (BBAT). During this practice, all the hatchery activities were conducted according to BBAT standar procedures. The number of broodstocks used in this practice was 225 females and 53 males. These broodstocks were propagated in mating pond for two weeks. Broodstocks having eggs were moved into hatching tank individually. Afterward, broodstock was removed into another tank while larvae were kept in hatching tank for 10 days. Larvae were cultivated in concrete tank for one month. Data collected included surviral rate, weight and length of lobster, and water quality. Survival rate of larvae was 50%. Water quality in broodstock tank was temperature 28,4 0C, pH 7,5; DO 5,6 mg/l, in hatching pond was temperature 27,23 0C, pH 7,5; DO 5,2 mg/l. In general, lobster hatchery consisted of tank preparation, boroodstock culture, broodstock seletion, hatching, larvae rearing, feeding, water quality contol, pest and disease prevention, harvest. Key Words: Hatchery, lobster, broodstocks.
Purification of carp (Cyprinus carpio) kidney cathepsin C Pangkey, Henneke; Lantu, Sartje
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
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Abstract

Pemurnian enzim cathepsin C diperoleh melalui penggunaan colom kromatografi S-Sepharose FF, Sephacryl S-200 HR, Concanavalin A-Agarose, Affi-gel 501 dan DEAE-Sephacel. Dari 160 g ginjal ikan mas diperoleh 0,33 mg enzim cathepsin C, dengan hasil pemurnian 150 kali. Berat molekul cathepsin C adalah 170 kDa yang terdiri dari 5 subunit, dengan berat molekul berkisar antara 10 sampai 20 kDa. Cathepsin C adalah oligomerik protein dan aktif sebagai eksopeptidase (pH 5) maupun endopeptidase (pH 7)
Kesesuaian areal budidaya rumput laut berdasarkan kapasitas perairan Desa Arakan Kabupeten Minahasa Selatan Burase, Handy; Rompas, Robert; Ngangi, Edwin L.A.
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
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Abstract

The purpose of this research was to provide data on waters physical and chemical parameters, proper areal map, carrying capacity, and potential production of seaweed. Research method used was spacial approach through direct measurement in the field and through laboratory analysis. Ten observation stations were established purposively at Desa Arakan waters. Samples were collected four times at two weeks interval. It was found that water temperature was 31–31,25 ºC, flow rate 5,97–11,09 cm/det., dissolved oxygen 5,34–6,45 mg/l, pH 7,7–8,1, salinity 32,5-33 ppt, nitrate 5,02–5,25 mg/l, phosphate 0,01–0,14 mg/l, water depth 1,3–16,5 m, velocity 1,3–4,5 m, tide 0–283 cm with Mean Sea Level 75,6 cm. bottom substrate consisted of coral sand and muddy sand. Proper culture area based on SIG analysis was 134.64 ha in which very proper areal was 68.40 ha, proper 66.23%. while proper areal was 66.23 ha. Based on carrying capacity analysis, proper sustainable site was 126.57 ha. This area could support 1.054 long line measuring 20x60 cm each. Seaweed potential was 3363,7 ton per ha or 4.4 ton/ha/year or 4.4 ton/ha/planting season. Keywords: marine seaweed, water quality, SIG, sea water, SIG, carrying capacity, production

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